FastQCFastQC Report
Fri 27 May 2016
SRR2139639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8597007
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109300.053.0467571
GTATCAA201700.037.787391
TCAACGC250950.029.6111094
ATCAACG252050.029.4989433
CAACGCA258250.028.857685
TATCAAC261750.028.60892
AACGCAG264700.028.1869936
ACGCAGA310300.023.9899087
CGCAGAG319600.023.2650538
GTGGTAT55050.022.4240281
TGGTATC52200.022.3299562
GCAGAGT366250.020.1256089
GTACATG264650.020.1204571
TACATGG265350.019.6459312
GAGTACT212950.019.27565212-13
ACATGGG269000.018.9436743
AGAGTAC332050.018.59802210-11
CAGAGTA360000.018.05599810-11
GTACTTT238800.017.2072314-15
AGTACTT234750.017.14671912-13
Produced by FastQC (version 0.11.5)