##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8597007
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48449501087995	34.0	31.0	34.0	31.0	34.0
2	32.612208760560506	34.0	31.0	34.0	31.0	34.0
3	32.668601526089255	34.0	31.0	34.0	31.0	34.0
4	36.19811743784785	37.0	35.0	37.0	35.0	37.0
5	36.11484729511096	37.0	35.0	37.0	35.0	37.0
6	36.1524408436564	37.0	36.0	37.0	35.0	37.0
7	36.1060348095564	37.0	36.0	37.0	35.0	37.0
8	36.080068912355195	37.0	36.0	37.0	35.0	37.0
9	37.79527561161693	39.0	38.0	39.0	35.0	39.0
10-11	37.72255594301598	39.0	37.5	39.0	35.0	39.0
12-13	37.67011408737948	39.0	37.0	39.0	35.0	39.0
14-15	39.01000557519611	40.0	38.0	41.0	36.0	41.0
16-17	38.978397656300615	40.0	38.0	41.0	35.5	41.0
18-19	38.97579436657432	40.0	38.0	41.0	35.0	41.0
20-21	38.945280510702425	40.0	38.0	41.0	35.0	41.0
22-23	38.84113057870548	40.0	38.0	41.0	35.0	41.0
24-25	38.71594237101103	40.0	38.0	41.0	34.0	41.0
26-27	38.64962839740888	40.0	38.0	41.0	34.5	41.0
28-29	38.593664436862866	40.0	38.0	41.0	34.0	41.0
30-31	38.52475138987432	40.0	38.0	41.0	34.0	41.0
32-33	38.41737138454534	40.0	38.0	41.0	34.0	41.0
34-35	38.30744126528667	40.0	38.0	41.0	34.0	41.0
36-37	38.44600715167425	40.0	38.0	41.0	34.0	41.0
38-39	38.514092533042955	40.0	38.0	41.0	34.0	41.0
40-41	38.44436293691588	40.0	38.0	41.0	34.0	41.0
42-43	38.40434688586129	40.0	38.0	41.0	34.0	41.0
44-45	38.368191999538965	40.0	38.0	41.0	34.0	41.0
46-47	38.27574371699016	40.0	38.0	41.0	33.0	41.0
48-49	38.19548707589516	40.0	38.0	41.0	33.0	41.0
50-51	38.12318475213466	40.0	37.5	41.0	33.0	41.0
52-53	38.046234763207124	40.0	37.0	41.0	33.0	41.0
54-55	37.916951652723	40.0	37.0	41.0	33.0	41.0
56-57	37.75369950016187	40.0	36.5	41.0	33.0	41.0
58-59	37.620790539632765	39.0	36.0	41.0	33.0	41.0
60-61	37.464433391017224	39.0	36.0	41.0	32.5	41.0
62-63	37.23348611323097	39.0	35.0	40.5	32.0	41.0
64-65	36.89760754087178	38.5	35.0	40.0	31.5	41.0
66-67	36.58891776132998	38.0	35.0	40.0	31.0	41.0
68-69	36.299770738396106	37.0	35.0	40.0	31.0	41.0
70-71	35.9820827539042	37.0	35.0	39.0	31.0	41.0
72-73	35.662999003031075	36.0	34.0	39.0	31.0	40.5
74-75	35.33233214440026	36.0	34.0	39.0	30.0	40.0
76-77	34.3564680199858	35.0	33.5	37.0	29.5	39.0
78-79	34.67562580806959	35.0	34.0	37.0	30.0	39.0
80-81	34.52632138570033	35.0	34.0	37.0	30.0	39.0
82-83	34.29367853077287	35.0	34.0	36.0	30.0	38.5
84-85	34.039449801976474	35.0	34.0	36.0	30.0	37.0
86-87	33.823560181733	35.0	34.0	35.5	30.0	37.0
88-89	33.62714398485829	35.0	33.0	35.0	30.0	36.5
90-91	33.467034682909244	35.0	33.0	35.0	29.0	36.0
92-93	33.34810886354451	35.0	33.0	35.0	29.0	36.0
94-95	33.295113793026324	35.0	33.0	35.0	29.0	36.0
96-97	33.31545312185402	35.0	33.0	35.0	29.5	35.0
98-99	33.36358090126323	35.0	33.0	35.0	30.0	35.0
100-101	31.890117344518607	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	7.0
20	40.0
21	268.0
22	1276.0
23	4270.0
24	10992.0
25	22615.0
26	39877.0
27	62572.0
28	89015.0
29	118700.0
30	150807.0
31	187940.0
32	234696.0
33	303094.0
34	414683.0
35	590458.0
36	972631.0
37	1853183.0
38	2397687.0
39	1087487.0
40	54703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.704637405161755	21.61526931585252	15.68525135378526	23.994841925200472
2	13.8565913118587	21.96857687754099	41.110266441049006	23.064565369551296
3	16.948225928887336	26.80990335472605	31.511796083427186	24.73007463295943
4	12.088080431722393	19.078327966736328	39.1628085405547	29.670783060986583
5	12.859697899478029	38.11166352565068	35.89582233628777	13.132816238583525
6	29.10834306734228	38.86490250210364	18.864962988277547	13.161791442276533
7	25.763713553299834	33.17065266675399	23.490631505898683	17.5750022740475
8	24.22515184645075	36.783010645449046	21.35720024422453	17.63463726387567
9	25.19374475326122	16.982549857177037	21.637704843092486	36.18600054646925
10-11	23.69581910570536	27.195305233182854	29.26171776786874	19.847157893243047
12-13	24.63275814236718	24.785878091464845	29.276171344055474	21.305192422112505
14-15	21.851198286565978	26.161792750021245	26.987766494713362	24.999242468699418
16-17	21.296216380446868	29.33213609482185	27.650696067910154	21.72095145682113
18-19	21.35639430750659	28.389534682190522	29.4745752183958	20.77949579190709
20-21	22.150820462366074	27.483732783295245	29.422927626734698	20.94251912760398
22-23	21.89885771750456	27.39007094921248	29.26058667333017	21.450484659952792
24-25	21.677871970662476	27.577556887851056	29.306605451796635	21.437965689689833
26-27	21.661741070622977	27.704449102594626	29.220931173405624	21.412878653376772
28-29	21.585512005191205	27.703766633085507	29.279248099808182	21.431473261915105
30-31	21.786669756284567	27.603284644182057	29.209322820682402	21.400722778850973
32-33	21.66781106963049	27.77628196652425	29.051675444586788	21.504231519258468
34-35	21.830563063778584	27.699118094289314	29.0959250209577	21.374393820974394
36-37	21.719356516420795	27.683884632731203	29.060832020184257	21.535926830663744
38-39	21.591788544928583	27.791094130073486	29.110091677066052	21.507025647931883
40-41	21.841877960649512	27.68075836283578	28.955331657399046	21.522032019115663
42-43	21.779614069593414	27.746913983617194	28.747256587546705	21.726215359242687
44-45	21.834579994873916	27.873322220804326	28.544421871660514	21.747675912661247
46-47	21.954219338863744	27.846294925551074	28.243864751019487	21.955620984565698
48-49	21.888408944072648	27.975903167629667	28.19695481164619	21.93873307665149
50-51	21.90322553194743	28.051944752524555	28.076480851298413	21.9683488642296
52-53	22.036193313612927	27.99983411118549	27.976980940294055	21.986991634907525
54-55	22.012742350654854	27.94131885560742	27.98728577705068	22.058653016687042
56-57	22.034194276554103	28.05068341386591	27.920575615389232	21.994546694190753
58-59	21.927983615751835	28.071264295010906	28.03719496990025	21.96355711933701
60-61	22.064548123145343	28.089327563728272	27.93503275397327	21.911091559153114
62-63	22.02227524110113	28.191186679501307	27.89534907727365	21.89118900212391
64-65	22.00055793219861	28.07284152421453	27.91007007737762	22.01653046620924
66-67	22.053118872249364	28.15033756692049	27.699647998603353	22.09689556222679
68-69	21.995145106931556	28.086126143020135	27.822125727599804	22.0966030224485
70-71	21.99994646993002	27.89457983892547	27.904353436915585	22.201120254228925
72-73	22.048078040346095	27.926328352262892	27.797875883030272	22.227717724360744
74-75	21.94052550983257	28.004725898438075	27.78573837509465	22.2690102166347
76-77	22.06042173313654	27.936113797246794	27.733452697335082	22.27001177228159
78-79	22.012398766092872	27.92605057101163	27.712428424767864	22.349122238127634
80-81	22.018212588526932	27.9771983437496	27.68045041593641	22.324138651787056
82-83	22.113102109025697	27.921005130143712	27.725683505853603	22.240209254976993
84-85	22.16701389348878	27.924840323802314	27.7018014748652	22.206344307843707
86-87	22.066171113749427	27.90900579430266	27.792957014819603	22.231866077128313
88-89	22.098028941798812	27.858091595119706	27.783623172546466	22.260256290535015
90-91	22.141929242477552	27.868983589739027	27.8293053610994	22.15978180668402
92-93	22.200663929366975	27.795769976428534	27.83051928536851	22.173046808835977
94-95	22.019441830317426	27.788210036865557	27.925226319659558	22.267121813157463
96-97	22.01745902487963	27.834194888949526	27.93724892515436	22.21109716101648
98-99	22.182498606048107	28.411786506678517	28.029300719342277	21.376414167931106
100-101	22.409590332262404	31.279411077553064	25.316104038434585	20.99489455174995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2452.0
1	2017.5
2	2268.5
3	3089.5
4	3980.5
5	5959.0
6	7755.0
7	9745.5
8	11547.0
9	11927.0
10	11028.5
11	10048.5
12	11117.0
13	15142.0
14	24864.0
15	38097.0
16	48388.0
17	53829.0
18	54521.5
19	51306.5
20	46243.0
21	41294.0
22	38818.0
23	41036.0
24	47555.0
25	59233.0
26	75309.0
27	92953.0
28	113008.5
29	134874.0
30	155775.5
31	176340.5
32	197974.5
33	219631.0
34	240306.0
35	259054.5
36	278826.5
37	295235.5
38	307111.5
39	318433.5
40	328677.5
41	336974.5
42	340008.5
43	342419.5
44	345786.5
45	348408.0
46	349722.0
47	347206.5
48	341322.5
49	334921.0
50	327633.0
51	316003.5
52	300223.0
53	282825.5
54	264210.5
55	244208.0
56	222603.5
57	199737.0
58	176831.5
59	153257.5
60	128977.0
61	105051.5
62	82766.0
63	63524.0
64	46999.0
65	34448.0
66	26062.5
67	19901.0
68	15231.0
69	11175.0
70	8017.0
71	5782.0
72	4056.5
73	2782.5
74	1773.5
75	1078.0
76	697.5
77	451.5
78	292.0
79	199.0
80	143.5
81	98.0
82	73.5
83	57.0
84	40.0
85	25.0
86	15.0
87	10.5
88	6.5
89	4.0
90	3.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1631955167653114E-5
2	0.022507833249408776
3	1.1631955167653114E-5
4	1.1631955167653114E-5
5	1.1631955167653114E-5
6	1.1631955167653114E-5
7	1.1631955167653114E-5
8	0.0
9	0.0
10-11	3.5477463261342E-4
12-13	0.0017680571854832735
14-15	2.9079887919132785E-5
16-17	1.1631955167653114E-5
18-19	1.1631955167653114E-5
20-21	1.1632317789338493E-5
22-23	5.81660545464973E-6
24-25	0.008179673093488951
26-27	0.026537038664348943
28-29	0.3294596254328928
30-31	0.31572289039029633
32-33	0.3171556368294196
34-35	0.16930291705069905
36-37	0.02923845939685856
38-39	0.028094932316110353
40-41	0.010782360485270275
42-43	0.008544973082439716
44-45	0.003115003274962902
46-47	0.0020205133681533115
48-49	0.001823704002803853
50-51	0.0018252205772901371
52-53	2.855862153742235E-4
54-55	1.875180017281659E-5
56-57	1.8891760226534874E-4
58-59	4.1253669831267415E-4
60-61	0.0028473949855134777
62-63	0.0025612709891157594
64-65	0.0026229675499862444
66-67	0.0065003501578914675
68-69	0.009966172777847237
70-71	0.013950753437938498
72-73	0.013149082687203712
74-75	0.010195885542768044
76-77	0.004729710854243865
78-79	0.0019921173663285754
80-81	0.0018879167178419373
82-83	3.147800313563836E-4
84-85	3.6195840345070875E-5
86-87	0.0
88-89	7.726113438809145E-4
90-91	0.0024350666484526697
92-93	0.014783124289998329
94-95	3.507429944737713E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	159.0
20-21	533.0
22-23	1174.0
24-25	2618.0
26-27	3404.0
28-29	5516.0
30-31	13643.0
32-33	18811.0
34-35	27950.0
36-37	37370.0
38-39	43642.0
40-41	49034.0
42-43	60776.0
44-45	73231.0
46-47	72404.0
48-49	63635.0
50-51	56446.0
52-53	53242.0
54-55	57969.0
56-57	61544.0
58-59	63729.0
60-61	64120.0
62-63	66034.0
64-65	73363.0
66-67	77026.0
68-69	76246.0
70-71	74423.0
72-73	73449.0
74-75	75905.0
76-77	76156.0
78-79	81525.0
80-81	82597.0
82-83	81563.0
84-85	84684.0
86-87	89063.0
88-89	96236.0
90-91	103729.0
92-93	109083.0
94-95	123960.0
96-97	186485.0
98-99	765041.0
100-101	5369489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.22627725141962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2732923614836	77.83929935559745
2	6.90026124356035	11.899676781889205
3	1.6168846750881987	4.182538388331795
4	0.5944753146660193	2.050375732060684
5	0.2516354344287544	1.08487933676676
6	0.1342177868714773	0.694386006171117
7	0.06793962127378184	0.41007264342166905
8	0.041875954695862326	0.28886461438186495
9	0.02668621962078307	0.20709480346325235
>10	0.09078816091243722	1.1889555599156942
>50	0.0015883505843667365	0.08760992448486993
>100	3.3416700935810323E-4	0.05371103316196615
>500	2.0709805085573573E-5	0.01253582035382332
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1631955167653114E-5
2	0.0	1.1631955167653114E-5	0.0	0.0	1.1631955167653114E-5
3	0.0	1.1631955167653114E-5	0.0	0.0	1.1631955167653114E-5
4	0.0	3.4895865502959345E-5	0.0	0.0	1.1631955167653114E-5
5	0.0	3.4895865502959345E-5	0.0	0.0	2.326391033530623E-5
6	0.0	3.4895865502959345E-5	0.0	0.0	3.4895865502959345E-5
7	0.0	3.4895865502959345E-5	0.0	0.0	3.4895865502959345E-5
8	0.0	3.4895865502959345E-5	0.0	0.0	4.652782067061246E-5
9	0.0	1.0468759650887803E-4	0.0	2.326391033530623E-5	4.652782067061246E-5
10-11	0.0	1.0468759650887803E-4	0.0	3.4895865502959345E-5	4.652782067061246E-5
12-13	0.0	1.1050357409270458E-4	0.0	5.2343798254439014E-5	5.2343798254439014E-5
14-15	0.0	1.8611128268244983E-4	0.0	9.887161892505148E-5	9.305564134122491E-5
16-17	5.815977583826557E-6	1.8611128268244983E-4	0.0	1.221355292603577E-4	1.1631955167653114E-4
18-19	1.1631955167653114E-5	1.919272602662764E-4	0.0	1.919272602662764E-4	1.337674844280108E-4
20-21	1.1631955167653114E-5	1.9774323785010296E-4	0.0	2.442710585207154E-4	1.5121541717949048E-4
22-23	1.1631955167653114E-5	2.3845508093688886E-4	0.0	4.187503860355121E-4	1.8611128268244983E-4
24-25	2.326391033530623E-5	3.373266998619403E-4	0.0	0.0010003481444181678	2.3263910335306228E-4
26-27	2.326391033530623E-5	3.7222256536489966E-4	0.0	0.0029487006350000645	2.3263910335306228E-4
28-29	2.326391033530623E-5	3.7222256536489966E-4	0.0	0.013033605765355316	2.3263910335306228E-4
30-31	2.907988791913279E-5	3.8385452053255276E-4	0.0	0.01845991285106549	2.3845508093688886E-4
32-33	3.4895865502959345E-5	3.8385452053255276E-4	0.0	0.02084446366043438	2.6753496885602166E-4
34-35	3.4895865502959345E-5	4.303823412031652E-4	0.0	0.02424681054697292	2.9079887919132786E-4
36-37	3.4895865502959345E-5	4.769101618737777E-4	0.0	0.029504454282752127	2.9079887919132786E-4
38-39	3.4895865502959345E-5	4.8272613945760426E-4	0.0	0.038693698865198085	2.9079887919132786E-4
40-41	3.4895865502959345E-5	5.350699377120433E-4	0.0	0.05817140779343323	2.9079887919132786E-4
42-43	3.4895865502959345E-5	5.641498256311761E-4	0.0	0.06772705896366026	3.0824681194280756E-4
44-45	3.4895865502959345E-5	6.339415566370948E-4	0.0	0.08224373901289134	3.256947446942872E-4
46-47	3.4895865502959345E-5	6.979173100591869E-4	0.0	0.0988774349026353	3.3151072227811376E-4
48-49	4.07118430867859E-5	6.979173100591869E-4	0.0	0.11359185818971648	3.5477463261342E-4
50-51	4.652782067061246E-5	7.153652428106666E-4	0.0	0.12841096907330657	3.6059061019724656E-4
52-53	4.652782067061246E-5	7.211812203944931E-4	0.0	0.14801081353080203	3.7803854294872626E-4
54-55	4.652782067061246E-5	7.269971979783197E-4	0.0	0.19153758976816	3.954864757002059E-4
56-57	4.652782067061246E-5	7.328131755621462E-4	0.0	0.23687313503408802	3.954864757002059E-4
58-59	4.652782067061246E-5	7.328131755621462E-4	0.0	0.2724145740488521	3.954864757002059E-4
60-61	4.652782067061246E-5	7.328131755621462E-4	0.0	0.29272978374915826	3.954864757002059E-4
62-63	4.652782067061246E-5	7.502611083136259E-4	0.0	0.3089854410959535	3.954864757002059E-4
64-65	4.652782067061246E-5	8.723966375739836E-4	0.0	0.31777338322511545	3.954864757002059E-4
66-67	4.652782067061246E-5	8.840285927416367E-4	0.0	0.3235021211451846	4.07118430867859E-4
68-69	4.652782067061246E-5	8.898445703254633E-4	0.0	0.3300160160390704	4.187503860355121E-4
70-71	4.652782067061246E-5	9.072925030769429E-4	0.0	0.3358029137349778	4.7109418428995116E-4
72-73	4.652782067061246E-5	9.18924458244596E-4	0.0	0.3399497057522461	5.11806027376737E-4
74-75	4.652782067061246E-5	9.421883685799023E-4	0.0	0.34344510828012587	5.11806027376737E-4
76-77	4.652782067061246E-5	0.0010003481444181678	0.0	0.34697540667350857	5.11806027376737E-4
78-79	4.652782067061246E-5	0.001011980099585821	0.0	0.35007532272568814	5.11806027376737E-4
80-81	4.652782067061246E-5	0.001011980099585821	0.0	0.35133157388379466	5.176220049605636E-4
82-83	4.652782067061246E-5	0.001023612054753474	0.0	0.35179103611291695	5.234379825443901E-4
84-85	4.652782067061246E-5	0.0010352440099211271	0.0	0.35210509890244357	5.350699377120432E-4
86-87	4.652782067061246E-5	0.0010817718305917397	0.0	0.352227234431704	5.467018928796964E-4
88-89	4.652782067061246E-5	0.001105035740927046	0.0	0.35227376225237456	5.641498256311761E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10930	0.0	53.046757	1
GTATCAA	20170	0.0	37.78739	1
TCAACGC	25095	0.0	29.611109	4
ATCAACG	25205	0.0	29.498943	3
CAACGCA	25825	0.0	28.85768	5
TATCAAC	26175	0.0	28.6089	2
AACGCAG	26470	0.0	28.186993	6
ACGCAGA	31030	0.0	23.989908	7
CGCAGAG	31960	0.0	23.265053	8
GTGGTAT	5505	0.0	22.424028	1
TGGTATC	5220	0.0	22.329956	2
GCAGAGT	36625	0.0	20.125608	9
GTACATG	26465	0.0	20.120457	1
TACATGG	26535	0.0	19.645931	2
GAGTACT	21295	0.0	19.275652	12-13
ACATGGG	26900	0.0	18.943674	3
AGAGTAC	33205	0.0	18.598022	10-11
CAGAGTA	36000	0.0	18.055998	10-11
GTACTTT	23880	0.0	17.20723	14-15
AGTACTT	23475	0.0	17.146719	12-13
>>END_MODULE