FastQCFastQC Report
Fri 27 May 2016
SRR2139638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9039338
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127050.048.137281
GTATCAA222950.036.7485161
TCAACGC276700.029.1848954
ATCAACG279200.028.9235673
CAACGCA284000.028.4802045
TATCAAC287100.028.29822
AACGCAG293650.027.588276
ACGCAGA336950.024.018087
CGCAGAG344600.023.4973418
GCAGAGT392400.020.6350259
GTACATG265400.020.0360341
GAGTACT230600.019.73375912-13
GTGGTAT65750.019.6477181
TACATGG266800.019.5448272
AGAGTAC354300.018.94813510-11
ACATGGG272000.018.8042073
TGGTATC65000.018.7519452
CAGAGTA385200.018.29148510-11
GTACTTT256150.017.69833614-15
AGTACTT254200.017.49654212-13
Produced by FastQC (version 0.11.5)