##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9039338
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47987717684636	34.0	31.0	34.0	31.0	34.0
2	32.60565895422873	34.0	31.0	34.0	31.0	34.0
3	32.66085071716535	34.0	31.0	34.0	31.0	34.0
4	36.192379021561095	37.0	35.0	37.0	35.0	37.0
5	36.109700732509395	37.0	35.0	37.0	35.0	37.0
6	36.14533420478358	37.0	36.0	37.0	35.0	37.0
7	36.09862160259966	37.0	36.0	37.0	35.0	37.0
8	36.0710370604573	37.0	35.0	37.0	35.0	37.0
9	37.78497883362697	39.0	38.0	39.0	35.0	39.0
10-11	37.7110481431273	39.0	37.5	39.0	35.0	39.0
12-13	37.66000502470425	39.0	37.0	39.0	35.0	39.0
14-15	38.99744013333719	40.0	38.0	41.0	36.0	41.0
16-17	38.96701550489649	40.0	38.0	41.0	35.0	41.0
18-19	38.96029471405981	40.0	38.0	41.0	35.0	41.0
20-21	38.92856406609357	40.0	38.0	41.0	35.0	41.0
22-23	38.827169668125435	40.0	38.0	41.0	35.0	41.0
24-25	38.70063423137313	40.0	38.0	41.0	34.0	41.0
26-27	38.63422968550892	40.0	38.0	41.0	34.5	41.0
28-29	38.579219177884795	40.0	38.0	41.0	34.0	41.0
30-31	38.50754806303137	40.0	38.0	41.0	34.0	41.0
32-33	38.39854931831445	40.0	38.0	41.0	34.0	41.0
34-35	38.29029650927242	40.0	38.0	41.0	34.0	41.0
36-37	38.433922648405954	40.0	38.0	41.0	34.0	41.0
38-39	38.50208749903736	40.0	38.0	41.0	34.0	41.0
40-41	38.4302527474904	40.0	38.0	41.0	34.0	41.0
42-43	38.39051908703569	40.0	38.0	41.0	34.0	41.0
44-45	38.35190052134747	40.0	38.0	41.0	34.0	41.0
46-47	38.26021491781	40.0	38.0	41.0	33.0	41.0
48-49	38.17767374464246	40.0	38.0	41.0	33.0	41.0
50-51	38.106548163421266	40.0	37.0	41.0	33.0	41.0
52-53	38.02586940231292	40.0	37.0	41.0	33.0	41.0
54-55	37.89441671373419	40.0	37.0	41.0	33.0	41.0
56-57	37.73058686061496	40.0	36.5	41.0	33.0	41.0
58-59	37.59149039680602	39.0	36.0	41.0	33.0	41.0
60-61	37.43361796921359	39.0	36.0	41.0	32.0	41.0
62-63	37.20343547852386	39.0	35.0	40.5	32.0	41.0
64-65	36.86197533872202	38.5	35.0	40.0	31.5	41.0
66-67	36.54923270270194	38.0	35.0	40.0	31.0	41.0
68-69	36.25780020240681	37.0	35.0	40.0	31.0	41.0
70-71	35.93855313958181	37.0	35.0	39.0	31.0	41.0
72-73	35.61901188725771	36.0	34.0	39.0	31.0	40.5
74-75	35.290669237572374	36.0	34.0	39.0	30.0	40.0
76-77	34.31413047759921	35.0	33.0	37.0	29.0	39.0
78-79	34.634998126785916	35.0	34.0	37.0	30.0	39.0
80-81	34.48850305279986	35.0	34.0	37.0	30.0	39.0
82-83	34.25979103936392	35.0	34.0	36.0	30.0	38.5
84-85	34.00933371083235	35.0	34.0	36.0	30.0	37.0
86-87	33.796156795180906	35.0	34.0	35.5	30.0	37.0
88-89	33.600037254156184	35.0	33.0	35.0	29.5	36.5
90-91	33.44328056802169	35.0	33.0	35.0	29.0	36.0
92-93	33.3214026299397	35.0	33.0	35.0	29.0	36.0
94-95	33.269021345937546	35.0	33.0	35.0	29.0	36.0
96-97	33.29335357735824	35.0	33.0	35.0	29.5	35.0
98-99	33.34575362094972	35.0	33.0	35.0	30.0	35.0
100-101	31.87459067862544	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	34.0
21	261.0
22	1266.0
23	4247.0
24	11105.0
25	23478.0
26	41685.0
27	65380.0
28	94256.0
29	125989.0
30	160041.0
31	201022.0
32	250651.0
33	322679.0
34	439626.0
35	630047.0
36	1036097.0
37	1956780.0
38	2489473.0
39	1129847.0
40	55372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82019223312506	21.50465238766958	15.66747649744666	24.007678881758697
2	13.976699930665884	21.964441535052455	40.82638609033264	23.23247244394902
3	17.014400503045742	26.678261912350433	31.441465231354908	24.865872353248918
4	12.13402044862361	18.967663225743216	39.0165451293607	29.88177119627247
5	12.918480636356405	38.05803456603067	35.833546199240054	13.189938598372866
6	29.39757639304741	38.669240896760456	18.73559974586632	13.197582964325813
7	25.907386791752536	33.10052496106739	23.402203059804055	17.58988518737602
8	24.318539698371715	36.68951199744937	21.238778768976225	17.75316953520269
9	25.230984835393922	16.899943336558497	21.530249228428012	36.338822599619576
10-11	23.789315241678953	27.09133864922816	29.194768934694764	19.924577174398124
12-13	24.733497452338696	24.71438618966504	29.19891726814595	21.353199089850317
14-15	21.89127487399103	26.090377319176028	26.967139678891254	25.051208127941692
16-17	21.36573496864483	29.256058352945757	27.60458785809315	21.77361882031627
18-19	21.37750021074552	28.29969960189563	29.478253827879875	20.84454635947898
20-21	22.18555111365882	27.39824670860283	29.425360932305267	20.99084124543308
22-23	21.903000508558897	27.340796807181718	29.23409304055432	21.522109643705065
24-25	21.72173650519018	27.456119741332763	29.279529429523716	21.542614323953345
26-27	21.656857283916498	27.63708749411376	29.21754463936249	21.488510582607248
28-29	21.642718341103212	27.646142802866564	29.246310087173722	21.4648287688565
30-31	21.8059833820519	27.531348109661423	29.188094440367728	21.47457406791895
32-33	21.695687295772455	27.648378194739625	29.04079353837999	21.615140971107934
34-35	21.853784138895644	27.620008967629534	29.0733561018919	21.452850791582918
36-37	21.778653576569308	27.59681894891772	29.040507998580495	21.58401947593248
38-39	21.662414804738823	27.730281094848124	29.077903605921644	21.5294004944914
40-41	21.958551389532545	27.636175228966426	28.8624894148596	21.542783966641434
42-43	21.80335765286937	27.72134834936317	28.66532323250071	21.80997076526675
44-45	21.914538994123347	27.824107613418704	28.458176062521662	21.80317732993629
46-47	22.0137553097265	27.813619088635654	28.17370636688074	21.998919234757107
48-49	21.928936505418715	27.934052193374708	28.152140020371448	21.984871280835133
50-51	21.970432502992736	28.0400591439381	28.019940837232248	21.969567515836914
52-53	22.13540274690533	27.94372618887478	27.869652070904934	22.051218993314958
54-55	22.124746604934277	27.859389257248644	27.918280803653296	22.097583334163787
56-57	22.115721964579812	27.97911993142114	27.850134712350393	22.05502339164866
58-59	22.048153638978196	27.969231669953924	27.922266789732554	22.060347901335327
60-61	22.098548279605676	27.976144135890696	27.926805465947336	21.99850211855629
62-63	22.08385379108356	28.085743302998665	27.83371186206255	21.996691043855222
64-65	22.07750040003915	27.966406032745727	27.850517409859137	22.105576157355983
66-67	22.119680298745877	28.03767952452626	27.67765485490284	22.164985321825025
68-69	22.117603845554477	27.9776560260242	27.74718563054126	22.15755449788006
70-71	22.060378953455118	27.820910398156506	27.823535252725158	22.295175395663218
72-73	22.117202464010155	27.85553223148266	27.728494600189375	22.29877070431781
74-75	22.06388008363694	27.92122207013052	27.68687979795882	22.328018048273723
76-77	22.17702902152757	27.863848786165374	27.655849776630564	22.303272415676485
78-79	22.116220029864735	27.85313339545369	27.627493486435455	22.403153088246125
80-81	22.143296402671293	27.891540746644523	27.58442998718117	22.380732863503017
82-83	22.205531329984858	27.82376893331137	27.688404105954938	22.282295630748834
84-85	22.223932665887368	27.833824159300825	27.688537739178383	22.253705435633425
86-87	22.146774912392623	27.782205870786765	27.770279032779577	22.300740184041036
88-89	22.196656044017622	27.76169806149112	27.725347787673876	22.31629810681738
90-91	22.208076334599323	27.803443007292227	27.741709398453136	22.24677125965531
92-93	22.264247517155216	27.698511089229328	27.799111786554153	22.238129607061303
94-95	22.114340320131976	27.69208000193765	27.864581746792283	22.32899793113809
96-97	22.115175959879938	27.763379680886867	27.871319266575128	22.250125092658067
98-99	22.266655842450046	28.309148172131632	27.987523349607642	21.436672635810687
100-101	22.54706737264164	31.192699186255897	25.185555990383474	21.074677450718994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2574.0
1	2126.5
2	2398.5
3	3323.0
4	4335.0
5	6338.5
6	8136.5
7	10298.0
8	12170.5
9	12463.5
10	11555.5
11	10641.0
12	11906.5
13	16408.5
14	27141.0
15	41503.0
16	52505.5
17	58189.5
18	58862.5
19	55492.5
20	50249.5
21	45322.0
22	42570.5
23	44265.5
24	51235.5
25	63490.5
26	79649.5
27	97806.5
28	118863.0
29	141707.5
30	162268.0
31	182483.0
32	204634.5
33	226392.5
34	247479.5
35	267278.0
36	287876.0
37	305034.5
38	318507.5
39	331387.5
40	342004.5
41	351530.0
42	355863.0
43	358984.0
44	362326.0
45	364239.5
46	365928.5
47	365139.0
48	361537.0
49	356177.5
50	349720.5
51	337713.5
52	321524.5
53	302884.5
54	282547.5
55	262398.5
56	239658.0
57	215751.0
58	191617.0
59	165757.5
60	138437.5
61	112120.0
62	88719.0
63	68006.5
64	50486.5
65	37515.0
66	28582.5
67	22173.0
68	17094.5
69	12579.5
70	9148.5
71	6644.0
72	4673.5
73	3406.5
74	2403.5
75	1584.0
76	1075.5
77	707.0
78	456.0
79	273.5
80	159.0
81	107.0
82	89.0
83	64.0
84	42.5
85	27.5
86	16.0
87	16.0
88	12.5
89	9.0
90	9.5
91	9.0
92	6.5
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.106275702933113E-5
2	0.02146174863690239
3	1.106275702933113E-5
4	1.106275702933113E-5
5	1.106275702933113E-5
6	1.106275702933113E-5
7	1.106275702933113E-5
8	0.0
9	0.0
10-11	3.540082249385962E-4
12-13	0.0018308862883543021
14-15	1.6594135543996695E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.1064045277171207E-5
24-25	0.008266238843620688
26-27	0.026632529210272454
28-29	0.3291815483581537
30-31	0.3157364237411488
32-33	0.3168500563453717
34-35	0.16886682736973227
36-37	0.02591579819121477
38-39	0.024294463502413436
40-41	0.009219217572009462
42-43	0.006805981758604279
44-45	0.002132375338872847
46-47	0.0018284375856176026
48-49	0.001739721645704286
50-51	0.0016534515094158767
52-53	1.481480164610224E-4
54-55	1.1934651813992483E-5
56-57	1.9240719133903076E-4
58-59	2.6061228371150196E-4
60-61	0.0019248677081199045
62-63	9.364301324241582E-4
64-65	0.0012428246296770987
66-67	0.0063238575430633985
68-69	0.009676196569113454
70-71	0.013743385955501387
72-73	0.013415846500474825
74-75	0.010488133127976645
76-77	0.004929644340349245
78-79	0.002027942918743529
80-81	0.0019904618418006568
82-83	2.935137154863707E-4
84-85	6.906232356734526E-6
86-87	0.0
88-89	5.950896180169333E-4
90-91	0.002422417584250174
92-93	0.015247729599917976
94-95	4.0862939089925885E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	156.0
20-21	628.0
22-23	1267.0
24-25	2421.0
26-27	3867.0
28-29	6172.0
30-31	15320.0
32-33	21588.0
34-35	31684.0
36-37	42601.0
38-39	49249.0
40-41	55080.0
42-43	67022.0
44-45	80612.0
46-47	79043.0
48-49	69611.0
50-51	61497.0
52-53	57270.0
54-55	62227.0
56-57	65538.0
58-59	67328.0
60-61	66816.0
62-63	67924.0
64-65	74817.0
66-67	79372.0
68-69	79611.0
70-71	78184.0
72-73	76502.0
74-75	79861.0
76-77	79614.0
78-79	84630.0
80-81	85641.0
82-83	84919.0
84-85	86820.0
86-87	92281.0
88-89	99858.0
90-91	108234.0
92-93	112649.0
94-95	129929.0
96-97	195277.0
98-99	803615.0
100-101	5632603.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.3738039756872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9709729557611	76.81164208627001
2	7.03342040074629	12.009397091438261
3	1.6716939568196785	4.281566165305925
4	0.6130112372988211	2.093404048321721
5	0.27081654602939004	1.156031935704291
6	0.1476209038240338	0.7561774863472103
7	0.0829508877247707	0.49572829797566687
8	0.05604729381997215	0.38279765407632377
9	0.0340193248457198	0.2613923253687337
>10	0.11662328516738589	1.5326532701224884
>50	0.0023660417585473483	0.13415712758510584
>100	4.150443570108991E-4	0.058782984451385425
>500	4.212184725999658E-5	0.026269527032859168
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.531378514665565E-5	0.0	0.0	0.0
3	0.0	5.531378514665565E-5	0.0	0.0	0.0
4	0.0	7.743929920531791E-5	0.0	0.0	0.0
5	0.0	8.850205623464905E-5	0.0	1.106275702933113E-5	0.0
6	2.212551405866226E-5	8.850205623464905E-5	0.0	1.106275702933113E-5	0.0
7	2.212551405866226E-5	8.850205623464905E-5	0.0	1.106275702933113E-5	0.0
8	2.212551405866226E-5	8.850205623464905E-5	0.0	1.106275702933113E-5	0.0
9	2.212551405866226E-5	1.4381584138130468E-4	0.0	2.212551405866226E-5	0.0
10-11	2.212551405866226E-5	1.4381584138130468E-4	0.0	2.7656892573327824E-5	0.0
12-13	2.212551405866226E-5	1.5487859841063583E-4	0.0	3.318827108799339E-5	0.0
14-15	2.212551405866226E-5	2.4338065464528486E-4	0.0	3.318827108799339E-5	0.0
16-17	4.425102811732452E-5	2.54443411674616E-4	0.0	6.084516366132122E-5	0.0
18-19	4.425102811732452E-5	2.821003042479438E-4	0.0	8.850205623464905E-5	0.0
20-21	4.425102811732452E-5	3.2635133236526834E-4	0.0	1.1615894880797687E-4	0.0
22-23	4.425102811732452E-5	4.148533885999174E-4	0.0	3.871964960265895E-4	5.531378514665565E-6
24-25	4.425102811732452E-5	4.5357303820257633E-4	0.0	0.0011892463806530964	1.106275702933113E-5
26-27	4.425102811732452E-5	4.5357303820257633E-4	0.0	0.0035843332775032863	1.106275702933113E-5
28-29	4.425102811732452E-5	4.646357952319075E-4	0.0	0.016157156641338117	1.106275702933113E-5
30-31	4.425102811732452E-5	5.310123374078942E-4	0.0	0.022374426091822212	1.106275702933113E-5
32-33	4.425102811732452E-5	5.420750944372253E-4	0.0	0.025134583970640327	1.106275702933113E-5
34-35	4.425102811732452E-5	6.195143936425433E-4	0.0	0.029266523721095505	2.212551405866226E-5
36-37	4.425102811732452E-5	7.135478283918579E-4	0.0	0.03552804419969692	2.212551405866226E-5
38-39	4.425102811732452E-5	7.356733424505201E-4	0.0	0.04556749620381492	2.212551405866226E-5
40-41	4.425102811732452E-5	8.13112641655838E-4	0.0	0.06764875923435987	2.212551405866226E-5
42-43	4.425102811732452E-5	8.573636697731626E-4	0.0	0.07824688046845908	2.212551405866226E-5
44-45	4.978240663199009E-5	9.569284830371427E-4	0.0	0.09355773619705338	2.212551405866226E-5
46-47	5.531378514665565E-5	0.0010398991607571263	0.0	0.11133005536467383	2.7656892573327824E-5
48-49	5.531378514665565E-5	0.0010509619177864575	0.0	0.12687876036939874	3.318827108799339E-5
50-51	7.190792069065234E-5	0.0011284012169917753	0.0	0.14213430231284635	3.318827108799339E-5
52-53	7.743929920531791E-5	0.0011284012169917753	0.0	0.1631037582619435	3.318827108799339E-5
54-55	7.743929920531791E-5	0.0011394639740211062	0.0	0.20764794944054532	3.318827108799339E-5
56-57	7.743929920531791E-5	0.0011615894880797686	0.0	0.25414471723482407	3.318827108799339E-5
58-59	7.743929920531791E-5	0.0011671208665944342	0.0	0.29088413332923274	3.318827108799339E-5
60-61	7.743929920531791E-5	0.0012279660302557554	0.0	0.3116599910303166	3.318827108799339E-5
62-63	8.850205623464905E-5	0.0012832798154024112	0.0	0.329963322535345	3.318827108799339E-5
64-65	8.850205623464905E-5	0.0014824094419303715	0.0	0.3389186243505885	3.318827108799339E-5
66-67	9.956481326398018E-5	0.0015211290915330305	0.0	0.34464360111326736	3.8719649602658956E-5
68-69	9.956481326398018E-5	0.001565380119650355	0.0	0.3505677075024742	4.425102811732452E-5
70-71	9.956481326398018E-5	0.0015985683907383482	0.0	0.35608249188159574	4.425102811732452E-5
72-73	9.956481326398018E-5	0.0016040997692530138	0.0	0.36054078296441616	4.425102811732452E-5
74-75	9.956481326398018E-5	0.0016317566618263418	0.0	0.36410852210637545	4.425102811732452E-5
76-77	9.956481326398018E-5	0.0016815390684583318	0.0	0.3671175920183536	4.425102811732452E-5
78-79	9.956481326398018E-5	0.0017091959610316596	0.0	0.36963436924252635	4.425102811732452E-5
80-81	9.956481326398018E-5	0.0017147273395463252	0.0	0.3708291470016941	4.978240663199009E-5
82-83	9.956481326398018E-5	0.0017202587180609908	0.0	0.3713048455539554	5.531378514665565E-5
84-85	9.956481326398018E-5	0.0017257900965756564	0.0	0.37160353999374734	5.531378514665565E-5
86-87	9.956481326398018E-5	0.0017479156106343186	0.0	0.37169204204998196	5.531378514665565E-5
88-89	9.956481326398018E-5	0.0017921666387516431	0.0	0.37169757342849663	5.531378514665565E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12705	0.0	48.13728	1
GTATCAA	22295	0.0	36.748516	1
TCAACGC	27670	0.0	29.184895	4
ATCAACG	27920	0.0	28.923567	3
CAACGCA	28400	0.0	28.480204	5
TATCAAC	28710	0.0	28.2982	2
AACGCAG	29365	0.0	27.58827	6
ACGCAGA	33695	0.0	24.01808	7
CGCAGAG	34460	0.0	23.497341	8
GCAGAGT	39240	0.0	20.635025	9
GTACATG	26540	0.0	20.036034	1
GAGTACT	23060	0.0	19.733759	12-13
GTGGTAT	6575	0.0	19.647718	1
TACATGG	26680	0.0	19.544827	2
AGAGTAC	35430	0.0	18.948135	10-11
ACATGGG	27200	0.0	18.804207	3
TGGTATC	6500	0.0	18.751945	2
CAGAGTA	38520	0.0	18.291485	10-11
GTACTTT	25615	0.0	17.698336	14-15
AGTACTT	25420	0.0	17.496542	12-13
>>END_MODULE