FastQCFastQC Report
Fri 27 May 2016
SRR2139637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11666924
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140200.051.3225561
GTATCAA257350.037.071241
TCAACGC323750.028.8868714
ATCAACG328650.028.4948123
CAACGCA333900.028.0723675
TATCAAC341200.027.663252
AACGCAG344750.027.2011496
ACGCAGA400350.023.4133247
CGCAGAG412700.022.651168
GTGGTAT71300.022.2612271
TGGTATC68400.021.5961692
GCAGAGT469300.019.7299489
GTACATG342050.019.3285311
GAGTACT272800.018.77785312-13
TACATGG347200.018.7126832
AGAGTAC424500.018.44763210-11
ACATGGG343700.018.3452993
CAGAGTA462650.017.67649510-11
GTACTTT300400.016.9612414-15
AGTACTT301950.016.48854612-13
Produced by FastQC (version 0.11.5)