##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11666924
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47501989384691	34.0	31.0	34.0	31.0	34.0
2	32.60249788204671	34.0	31.0	34.0	31.0	34.0
3	32.65972907683293	34.0	31.0	34.0	31.0	34.0
4	36.18991381104394	37.0	35.0	37.0	35.0	37.0
5	36.10597420536896	37.0	35.0	37.0	35.0	37.0
6	36.14034144732579	37.0	36.0	37.0	35.0	37.0
7	36.090438576611966	37.0	36.0	37.0	35.0	37.0
8	36.06137127489645	37.0	35.0	37.0	35.0	37.0
9	37.77349136756184	39.0	38.0	39.0	35.0	39.0
10-11	37.69864970406938	39.0	37.5	39.0	35.0	39.0
12-13	37.65036148345528	39.0	37.0	39.0	35.0	39.0
14-15	38.99217167267054	40.0	38.0	41.0	35.5	41.0
16-17	38.962157634694464	40.0	38.0	41.0	35.0	41.0
18-19	38.953626722862	40.0	38.0	41.0	35.0	41.0
20-21	38.91897373847122	40.0	38.0	41.0	35.0	41.0
22-23	38.81652193655969	40.0	38.0	41.0	35.0	41.0
24-25	38.69487201713573	40.0	38.0	41.0	34.0	41.0
26-27	38.62508803254841	40.0	38.0	41.0	34.5	41.0
28-29	38.566594931035254	40.0	38.0	41.0	34.0	41.0
30-31	38.49855572090771	40.0	38.0	41.0	34.0	41.0
32-33	38.39531089520683	40.0	38.0	41.0	34.0	41.0
34-35	38.28739185721997	40.0	38.0	41.0	34.0	41.0
36-37	38.43257534658283	40.0	38.0	41.0	34.0	41.0
38-39	38.49994659341991	40.0	38.0	41.0	34.0	41.0
40-41	38.42602664117592	40.0	38.0	41.0	34.0	41.0
42-43	38.3868369064142	40.0	38.0	41.0	34.0	41.0
44-45	38.35552952382238	40.0	38.0	41.0	34.0	41.0
46-47	38.25979562427164	40.0	38.0	41.0	33.0	41.0
48-49	38.17704262125858	40.0	38.0	41.0	33.0	41.0
50-51	38.10646306039855	40.0	37.0	41.0	33.0	41.0
52-53	38.02365706338799	40.0	37.0	41.0	33.0	41.0
54-55	37.89047429399976	40.0	37.0	41.0	33.0	41.0
56-57	37.72483670034134	40.0	36.5	41.0	33.0	41.0
58-59	37.5851286378736	39.0	36.0	41.0	33.0	41.0
60-61	37.42441986364161	39.0	36.0	41.0	32.0	41.0
62-63	37.18867403614625	39.0	35.0	40.5	32.0	41.0
64-65	36.84409126077274	38.0	35.0	40.0	31.5	41.0
66-67	36.53029478908803	38.0	35.0	40.0	31.0	41.0
68-69	36.23392710290374	37.0	35.0	40.0	31.0	41.0
70-71	35.91241004820051	37.0	35.0	39.0	31.0	41.0
72-73	35.592089693825145	36.0	34.0	39.0	30.5	40.5
74-75	35.26196820476326	36.0	34.0	38.5	30.0	40.0
76-77	34.28922332135097	35.0	33.0	37.0	29.0	39.0
78-79	34.60507974012971	35.0	34.0	37.0	30.0	39.0
80-81	34.45983341601101	35.0	34.0	37.0	30.0	39.0
82-83	34.23606233564472	35.0	34.0	36.0	30.0	38.5
84-85	33.9895826563963	35.0	34.0	36.0	30.0	37.0
86-87	33.78177529057049	35.0	34.0	35.5	30.0	37.0
88-89	33.59410609451619	35.0	33.0	35.0	29.5	36.5
90-91	33.4429766245846	35.0	33.0	35.0	29.0	36.0
92-93	33.32533788934923	35.0	33.0	35.0	29.0	36.0
94-95	33.275180156842524	35.0	33.0	35.0	29.0	36.0
96-97	33.30024139831754	35.0	33.0	35.0	29.5	35.0
98-99	33.35479857609755	35.0	33.0	35.0	30.0	35.0
100-101	31.890401975795974	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	2.0
19	8.0
20	57.0
21	373.0
22	1795.0
23	5908.0
24	15230.0
25	31512.0
26	54976.0
27	86214.0
28	122538.0
29	163015.0
30	206411.0
31	258171.0
32	322500.0
33	412832.0
34	562292.0
35	800425.0
36	1309655.0
37	2475729.0
38	3167378.0
39	1577869.0
40	92032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.997606995024654	21.578109597210783	15.4533059750726	23.970977432691967
2	14.097343641146272	22.090716847039676	40.697831528745915	23.114107983068134
3	17.00338075487097	26.663761587140257	31.501918972452113	24.830938685536655
4	12.216470823793184	18.91603620183937	39.09524200943848	29.772250964928965
5	12.979534189011824	38.063710211117396	35.84578141910792	13.110974180762861
6	29.40593323636973	38.75256376553848	18.78116771340099	13.060335284690794
7	26.117507781187516	33.01692880238068	23.40656973677974	17.458993679652067
8	24.410420811039895	36.76454365902646	21.218379516175773	17.606656013757867
9	25.31332383011356	16.90554570386725	21.473091062656366	36.30803940336283
10-11	23.76972495567154	27.079242159195395	29.31227218101013	19.838760704122944
12-13	24.78176294237069	24.610013852640865	29.308781806313572	21.299441398674873
14-15	21.99286247018976	26.11497386007097	26.830111096573333	25.06205257316594
16-17	21.51318640256791	29.265744306971758	27.47357090324626	21.747498387214076
18-19	21.561287276749212	28.357680224881896	29.23206665270126	20.84896584566763
20-21	22.36587463575344	27.456420699593405	29.139489247376343	21.038215417276813
22-23	22.126869234866746	27.274254684841807	28.99122565642133	21.60765042387011
24-25	21.904226808587808	27.498140382573144	29.007622394981453	21.59001041385759
26-27	21.845866919488756	27.61777908829194	28.957633497394013	21.578720494825287
28-29	21.817647897198345	27.61026637385956	28.999693989394704	21.5723917395474
30-31	22.049459431848582	27.51303749548067	28.87506491656533	21.56243815610542
32-33	21.874909041662523	27.68094652465952	28.76026841357345	21.683876020104503
34-35	21.98231789920993	27.608242672198763	28.80839058396597	21.601048844625335
36-37	21.989767423695053	27.57654144996214	28.755479180092042	21.678211946250766
38-39	21.823625065884993	27.755765299976954	28.80661159882391	21.61399803531414
40-41	22.105280408958812	27.6178130722935	28.630732886736894	21.646173632010797
42-43	21.988651772920903	27.724827381708817	28.377676440568365	21.908844404801915
44-45	22.05348450330331	27.794138325275096	28.23532816970334	21.917049001718254
46-47	22.16784673804871	27.737676234344555	28.005015845022108	22.08946118258463
48-49	22.07899862883638	27.88494079377038	27.95760904626053	22.07845153113271
50-51	22.128188284996153	27.957910590019026	27.8467553185617	22.06714580642312
52-53	22.266285743648094	27.84859126222426	27.729248285795403	22.15587470833224
54-55	22.261610788582715	27.737846902433937	27.77632278978701	22.224219519196335
56-57	22.185777969564814	27.854145960151527	27.783711385665256	22.176364684618406
58-59	22.12785509656726	27.897441359815655	27.79125335636144	22.183450187255648
60-61	22.25686866019955	27.860938144520603	27.73292351482633	22.149269680453518
62-63	22.224465455705417	27.98616430503821	27.712975979771702	22.07639425948467
64-65	22.215611661323397	27.884265350560227	27.700444613162638	22.199678374953734
66-67	22.25345357519815	27.984900563873378	27.494609272640545	22.267036588287926
68-69	22.181098809829454	27.947502461692697	27.59698808667002	22.27441064180783
70-71	22.189426793395086	27.740948743804584	27.67856753648923	22.391056926311105
72-73	22.287018342671573	27.73752628967239	27.548346773538224	22.427108594117808
74-75	22.15492952268847	27.867546990275905	27.53677485762585	22.440748629409775
76-77	22.326019681102053	27.75893603731021	27.51216414999844	22.40288013158929
78-79	22.295216850708293	27.73603469925707	27.448122161538812	22.52062628849583
80-81	22.259915686982627	27.82062780462023	27.389622766644734	22.529833741752412
82-83	22.33487831088122	27.74774335902956	27.4887595023094	22.42861882777982
84-85	22.364726029287876	27.729422247347074	27.487785677139975	22.41806604622507
86-87	22.277098466440876	27.727891215858808	27.554296166832586	22.440714150867734
88-89	22.300518510242547	27.706061770805036	27.54883934577522	22.444580373177196
90-91	22.332961894483585	27.74513145945454	27.571398277374897	22.350508368686977
92-93	22.4221666456713	27.6172723463396	27.573537378949958	22.38702362903915
94-95	22.257019044973493	27.60834626772018	27.641413386912394	22.493221300393927
96-97	22.26710103298607	27.626670467168076	27.687770051722705	22.418458448123154
98-99	22.408697983022122	28.193782651916937	27.79460655618472	21.60291280887622
100-101	22.70742183304376	31.04856819926305	24.973941194568823	21.270068773124372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2944.0
1	2442.5
2	2739.5
3	3708.0
4	4813.5
5	7288.5
6	9530.0
7	11913.5
8	14181.5
9	14842.0
10	13969.5
11	12833.0
12	14068.5
13	18882.0
14	30675.5
15	47175.0
16	60434.5
17	67332.5
18	68191.5
19	64949.0
20	59754.5
21	54660.0
22	52249.5
23	55544.0
24	65221.5
25	81556.5
26	103581.5
27	128336.5
28	156575.0
29	186887.5
30	213849.0
31	240120.5
32	269263.5
33	299256.0
34	326294.0
35	350691.0
36	379368.5
37	403654.5
38	419981.5
39	434417.0
40	448655.5
41	462183.0
42	468568.5
43	473712.0
44	479397.0
45	482314.0
46	481643.0
47	476463.0
48	471263.0
49	465889.0
50	456691.0
51	442189.5
52	423950.5
53	402219.0
54	377179.5
55	350592.0
56	321582.0
57	290484.0
58	258905.5
59	224871.5
60	189142.5
61	154676.5
62	123365.0
63	95523.5
64	71173.0
65	53010.0
66	40221.5
67	30654.0
68	23408.5
69	17380.0
70	12553.5
71	9073.5
72	6468.5
73	4529.5
74	3108.0
75	2079.5
76	1356.0
77	843.0
78	544.5
79	360.5
80	237.0
81	155.0
82	106.0
83	70.5
84	48.5
85	35.5
86	26.0
87	20.5
88	14.0
89	8.0
90	5.0
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5713718543122418E-5
2	0.023673763538701376
3	2.5713718543122418E-5
4	2.5713718543122418E-5
5	2.5713718543122418E-5
6	2.5713718543122418E-5
7	2.5713718543122418E-5
8	8.571239514374139E-6
9	8.571239514374139E-6
10-11	3.514208200893397E-4
12-13	0.0016756773250601444
14-15	2.142809878593535E-5
16-17	4.2856197571870694E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008505284551068018
26-27	0.027938801716587424
28-29	0.3301417344157978
30-31	0.31577402024108076
32-33	0.31793714894359093
34-35	0.16888793725886764
36-37	0.02798203943515325
38-39	0.026772491478871143
40-41	0.009955139050579355
42-43	0.007493275084369055
44-45	0.0026308199801352993
46-47	0.0018686521682729402
48-49	0.0016549431647838305
50-51	0.0018123813614700785
52-53	2.086897774054546E-4
54-55	1.870030627361615E-5
56-57	1.367659770014333E-4
58-59	2.8556595459844E-4
60-61	0.0021957512741363275
62-63	0.0019451398764152225
64-65	0.0019940707995082336
66-67	0.0069346825715923
68-69	0.010866743811028676
70-71	0.015345844555108863
72-73	0.014670795198620884
74-75	0.011339383977465995
76-77	0.005550432975741355
78-79	0.0023736807552319347
80-81	0.002156888172986089
82-83	3.48739880208941E-4
84-85	0.0
86-87	0.0
88-89	6.247961957409234E-4
90-91	0.002637673421977245
92-93	0.015901095654778992
94-95	4.307621360800323E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	234.0
20-21	764.0
22-23	1595.0
24-25	3821.0
26-27	5082.0
28-29	8832.0
30-31	24179.0
32-33	33478.0
34-35	48069.0
36-37	65995.0
38-39	74246.0
40-41	81172.0
42-43	97040.0
44-45	115554.0
46-47	113709.0
48-49	100412.0
50-51	90363.0
52-53	84687.0
54-55	91467.0
56-57	95949.0
58-59	99163.0
60-61	99054.0
62-63	100189.0
64-65	109091.0
66-67	114350.0
68-69	114090.0
70-71	112761.0
72-73	110320.0
74-75	112496.0
76-77	113445.0
78-79	119261.0
80-81	120783.0
82-83	118339.0
84-85	122012.0
86-87	128352.0
88-89	137558.0
90-91	146980.0
92-93	152538.0
94-95	172916.0
96-97	254052.0
98-99	1008571.0
100-101	6963955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.06665713749047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84690330414608	76.42975718239053
2	7.040805491969512	11.978755735142608
3	1.755251578870933	4.479401527495574
4	0.6309212158680453	2.146814350040625
5	0.2770452544946217	1.1783656837831387
6	0.14170477304205195	0.7232610805868481
7	0.08783220105346973	0.5230114212852646
8	0.059283456566563746	0.40344363789385485
9	0.036437349071374074	0.27896431324082716
>10	0.1204921314775608	1.5924585561349145
>50	0.002668183206943217	0.15171566501664258
>100	6.042263639379291E-4	0.08502069992507985
>500	5.0833868872049896E-5	0.029030147064120604
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.428495805749656E-5	0.0	0.0	0.0
3	0.0	5.142743708624484E-5	0.0	8.57123951437414E-6	0.0
4	0.0	5.999867660061898E-5	0.0	8.57123951437414E-6	0.0
5	0.0	7.714115562936726E-5	0.0	8.57123951437414E-6	0.0
6	1.714247902874828E-5	7.714115562936726E-5	0.0	8.57123951437414E-6	8.57123951437414E-6
7	1.714247902874828E-5	7.714115562936726E-5	0.0	1.714247902874828E-5	8.57123951437414E-6
8	1.714247902874828E-5	1.1142611368686382E-4	0.0	1.714247902874828E-5	8.57123951437414E-6
9	1.714247902874828E-5	2.0570974834497937E-4	0.0	5.999867660061898E-5	8.57123951437414E-6
10-11	1.714247902874828E-5	2.0570974834497937E-4	0.0	7.714115562936726E-5	8.57123951437414E-6
12-13	1.714247902874828E-5	2.0570974834497937E-4	0.0	8.142677538655434E-5	8.57123951437414E-6
14-15	1.714247902874828E-5	3.04279002760282E-4	0.0	8.57123951437414E-5	1.285685927156121E-5
16-17	6.856991611499312E-5	3.128502422746561E-4	0.0	8.57123951437414E-5	1.714247902874828E-5
18-19	6.856991611499312E-5	3.4713520033215266E-4	0.0	1.1571173344405089E-4	2.571371854312242E-5
20-21	6.856991611499312E-5	3.7713453863246214E-4	0.0	2.142809878593535E-4	3.428495805749656E-5
22-23	6.856991611499312E-5	4.414188349902682E-4	0.0	6.042723857633769E-4	3.857057781468363E-5
24-25	6.856991611499312E-5	5.057031313480743E-4	0.0	0.0015642512113732804	4.28561975718707E-5
26-27	6.856991611499312E-5	5.399880894055709E-4	0.0	0.004718467352662964	4.28561975718707E-5
28-29	6.856991611499312E-5	5.399880894055709E-4	0.0	0.023103776110995494	4.28561975718707E-5
30-31	6.856991611499312E-5	6.214148647921251E-4	0.0	0.03230928734943332	4.7141817329057775E-5
32-33	6.856991611499312E-5	6.257004845493122E-4	0.0	0.03562635704149611	5.999867660061898E-5
34-35	6.856991611499312E-5	7.585546970221114E-4	0.0	0.04029768257683002	5.999867660061898E-5
36-37	6.856991611499312E-5	8.785520502233494E-4	0.0	0.047073247412942776	5.999867660061898E-5
38-39	6.856991611499312E-5	8.956945292520977E-4	0.0	0.060024390319162096	5.999867660061898E-5
40-41	6.856991611499312E-5	0.001045691220753645	0.0	0.08914089094949106	5.999867660061898E-5
42-43	6.856991611499312E-5	0.0010885474183255158	0.0	0.10154347452679044	5.999867660061898E-5
44-45	7.285553587218019E-5	0.0012128303912839408	0.0	0.11995449700366609	6.856991611499312E-5
46-47	7.714115562936726E-5	0.0013113996456992434	0.0	0.14176830156774828	8.57123951437414E-5
48-49	8.142677538655434E-5	0.0013242565049708045	0.0	0.15987504504186364	8.57123951437414E-5
50-51	9.856925441530261E-5	0.0014313969989004816	0.0	0.1770903796064841	8.57123951437414E-5
52-53	1.0285487417248969E-4	0.0014399682384148556	0.0	0.2032069464067821	1.1142611368686382E-4
54-55	1.0285487417248969E-4	0.0014485394779292296	0.0	0.2652755773501224	1.285685927156121E-4
56-57	1.0285487417248969E-4	0.001474253196472352	0.0	0.3309312720302283	1.285685927156121E-4
58-59	1.0285487417248969E-4	0.001474253196472352	0.0	0.37641455451325473	1.328542124727992E-4
60-61	1.0285487417248969E-4	0.0014828244359867263	0.0	0.3987597759272281	1.4571107174436037E-4
62-63	1.1142611368686382E-4	0.0015171093940442228	0.0	0.41186091552494897	1.4571107174436037E-4
64-65	1.1142611368686382E-4	0.001705676663360454	0.0	0.4214692750205624	1.4571107174436037E-4
66-67	1.285685927156121E-4	0.0017356760016607633	0.0	0.4277177086265411	1.4999669150154743E-4
68-69	1.285685927156121E-4	0.001774246579475447	0.0	0.43355472273582996	1.6285355077310867E-4
70-71	1.285685927156121E-4	0.0017871034387470083	0.0	0.43923745453386	1.6285355077310867E-4
72-73	1.285685927156121E-4	0.0017999602980185694	0.0	0.44345450437493206	1.7999602980185695E-4
74-75	1.285685927156121E-4	0.001829959636318879	0.0	0.447071567449998	1.7999602980185695E-4
76-77	1.285685927156121E-4	0.0019413857500057427	0.0	0.4506200606089489	1.7999602980185695E-4
78-79	1.285685927156121E-4	0.0019928131870919877	0.0	0.45380856170829603	1.7999602980185695E-4
80-81	1.285685927156121E-4	0.0020013844266063615	0.0	0.45515424631205276	1.885672693162311E-4
82-83	1.285685927156121E-4	0.0020056700463635486	0.0	0.4556428069643721	1.9713850883060522E-4
84-85	1.285685927156121E-4	0.002022812525392297	0.0	0.4560499408413049	2.0142412858779228E-4
86-87	1.328542124727992E-4	0.0020442406241782327	0.0	0.4561399388562058	2.0570974834497937E-4
88-89	1.3713983222998625E-4	0.0021599523576222835	0.0	0.45616993819450613	2.0570974834497937E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14020	0.0	51.322556	1
GTATCAA	25735	0.0	37.07124	1
TCAACGC	32375	0.0	28.886871	4
ATCAACG	32865	0.0	28.494812	3
CAACGCA	33390	0.0	28.072367	5
TATCAAC	34120	0.0	27.66325	2
AACGCAG	34475	0.0	27.201149	6
ACGCAGA	40035	0.0	23.413324	7
CGCAGAG	41270	0.0	22.65116	8
GTGGTAT	7130	0.0	22.261227	1
TGGTATC	6840	0.0	21.596169	2
GCAGAGT	46930	0.0	19.729948	9
GTACATG	34205	0.0	19.328531	1
GAGTACT	27280	0.0	18.777853	12-13
TACATGG	34720	0.0	18.712683	2
AGAGTAC	42450	0.0	18.447632	10-11
ACATGGG	34370	0.0	18.345299	3
CAGAGTA	46265	0.0	17.676495	10-11
GTACTTT	30040	0.0	16.96124	14-15
AGTACTT	30195	0.0	16.488546	12-13
>>END_MODULE