FastQCFastQC Report
Fri 27 May 2016
SRR2139636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8211202
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.053.6531331
GTATCAA195400.038.5000571
ATCAACG244550.030.128693
TCAACGC244000.030.1089864
CAACGCA251350.029.2974015
TATCAAC252950.029.2314432
AACGCAG256950.028.6588926
ACGCAGA297250.024.7589867
CGCAGAG305050.024.182218
GTGGTAT50500.021.852121
TGGTATC47650.020.9963992
GCAGAGT350800.020.9550199
GTACATG251850.019.8796351
GAGTACT204650.019.63836312-13
TACATGG252100.019.314882
ACATGGG250100.018.9876883
AGAGTAC317450.018.9635310-11
CAGAGTA340600.018.85964410-11
GTACTTT226500.017.658814-15
AGTACTT224000.017.41478512-13
Produced by FastQC (version 0.11.5)