##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8211202
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.941383368719954	34.0	31.0	34.0	31.0	34.0
2	33.07193855418488	34.0	33.0	34.0	31.0	34.0
3	33.130141725900785	34.0	33.0	34.0	31.0	34.0
4	36.483146194674056	37.0	37.0	37.0	35.0	37.0
5	36.448244483572566	37.0	37.0	37.0	35.0	37.0
6	36.47665615825795	37.0	37.0	37.0	35.0	37.0
7	36.45222843135512	37.0	37.0	37.0	35.0	37.0
8	36.44130092037682	37.0	37.0	37.0	35.0	37.0
9	38.237485327970255	39.0	39.0	39.0	37.0	39.0
10-11	38.17949132173341	39.0	39.0	39.0	37.0	39.0
12-13	38.15106874730399	39.0	39.0	39.0	37.0	39.0
14-15	39.67027196505457	41.0	40.0	41.0	37.0	41.0
16-17	39.64274402212977	41.0	40.0	41.0	37.0	41.0
18-19	39.65136334485499	41.0	40.0	41.0	37.0	41.0
20-21	39.64352405751811	41.0	40.0	41.0	37.0	41.0
22-23	39.586085645203674	41.0	40.0	41.0	37.0	41.0
24-25	39.52371138155764	41.0	39.5	41.0	37.0	41.0
26-27	39.47555926789675	41.0	39.0	41.0	37.0	41.0
28-29	39.30903177737021	41.0	39.0	41.0	36.0	41.0
30-31	39.22563912294298	41.0	39.0	41.0	36.0	41.0
32-33	39.12314434422745	41.0	39.0	41.0	35.5	41.0
34-35	39.02951394834044	40.5	39.0	41.0	35.0	41.0
36-37	39.11987497438959	40.5	39.0	41.0	35.0	41.0
38-39	39.14352779490612	41.0	39.0	41.0	35.0	41.0
40-41	39.134417300600674	41.0	39.0	41.0	35.0	41.0
42-43	39.090329514697345	41.0	39.0	41.0	35.0	41.0
44-45	39.0229983927437	40.0	39.0	41.0	35.0	41.0
46-47	38.93539689164524	40.0	38.5	41.0	35.0	41.0
48-49	38.85612447999564	40.0	38.0	41.0	35.0	41.0
50-51	38.76381836111476	40.0	38.0	41.0	35.0	41.0
52-53	38.655012946528245	40.0	38.0	41.0	35.0	41.0
54-55	38.52854973991998	40.0	38.0	41.0	35.0	41.0
56-57	38.35477135772316	40.0	37.5	41.0	34.0	41.0
58-59	38.18993231325847	40.0	37.0	41.0	34.0	41.0
60-61	37.992921660657515	39.5	36.5	41.0	34.0	41.0
62-63	37.74139909471332	39.0	36.0	41.0	34.0	41.0
64-65	37.43956336441673	39.0	35.5	40.0	33.5	41.0
66-67	37.113548376317	38.0	35.0	40.0	33.0	41.0
68-69	36.80097651715137	37.5	35.0	40.0	33.0	41.0
70-71	36.47182790095742	37.0	35.0	39.5	32.5	41.0
72-73	36.13055304784287	36.5	35.0	39.0	32.0	40.5
74-75	35.76192351885893	36.0	35.0	39.0	32.0	40.0
76-77	34.82960261220896	35.0	34.0	37.0	30.5	39.0
78-79	35.061800166152864	35.0	34.0	37.0	31.0	39.0
80-81	34.8833944827771	35.0	34.0	37.0	31.0	39.0
82-83	34.61753493226689	35.0	34.0	36.0	31.0	38.5
84-85	34.370292576343715	35.0	34.0	36.0	31.0	37.0
86-87	34.12195145717561	35.0	34.0	35.5	31.0	37.0
88-89	33.82391560626334	35.0	34.0	35.0	31.0	36.5
90-91	33.74720421706738	35.0	34.0	35.0	31.0	36.0
92-93	33.659624133678065	35.0	34.0	35.0	31.0	36.0
94-95	33.55326653014997	35.0	33.5	35.0	30.5	35.5
96-97	33.518451641536075	35.0	33.0	35.0	30.0	35.0
98-99	33.52025091851619	34.5	33.0	35.0	30.5	35.0
100-101	32.45070975418444	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	23.0
21	155.0
22	707.0
23	2339.0
24	5984.0
25	12489.0
26	22187.0
27	35191.0
28	50746.0
29	68758.0
30	88573.0
31	112352.0
32	143730.0
33	189117.0
34	275277.0
35	423736.0
36	806410.0
37	1914232.0
38	2718889.0
39	1268223.0
40	72077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69470966484801	20.87048178098207	15.150867108339828	24.283941445830084
2	13.878629992838665	21.442448689280894	41.207184231423156	23.471737086457285
3	16.970698558067028	26.466509158222916	31.062134645362434	25.500657638347622
4	12.034245908028058	18.538739770070148	38.97750633281372	30.449507989088076
5	13.166090218238505	37.787521921278255	35.577991036632895	13.468396823850352
6	29.692943784099768	38.20870274746688	18.732560405300077	13.365793063133284
7	26.307165822291505	32.86933700311769	23.12683895167576	17.696658222915044
8	24.617860407996346	36.32927753199562	21.12101506223024	17.931846997777793
9	25.525718882231978	16.839384327597053	21.30072577831336	36.334171011857606
10-11	24.044372192949975	26.876940924173258	28.927577416686745	20.15110946619002
12-13	24.949532358356304	24.471940892453993	28.966757851043003	21.611768898146703
14-15	22.15116278361716	25.787689279558496	26.748967660151056	25.312180276673292
16-17	21.66019087824795	28.94022212376469	27.339638377230425	22.05994862075693
18-19	21.67302058821595	28.026584901942492	29.159969514816463	21.14042499502509
20-21	22.497197215038625	27.134788640084658	29.117201442279555	21.25081270259716
22-23	22.23352621713495	27.029774178579302	28.952422942900956	21.784276661384794
24-25	22.003154847924673	27.219556232188964	29.008119539976178	21.76916937991019
26-27	21.935916936795145	27.36070317092564	28.926003449594536	21.777376442684684
28-29	21.904548477354616	27.35822220188914	28.973757874114835	21.763471446641404
30-31	22.130561314580333	27.23999135350669	28.881490898292807	21.74795643362017
32-33	21.961506371180164	27.39772721210641	28.788540368201478	21.85222604851195
34-35	22.05895598829567	27.4004122192701	28.77742768863661	21.76320410379762
36-37	22.05106827806531	27.31451937412979	28.7674029040558	21.8670094437491
38-39	21.94225314112021	27.467703888157306	28.786999128704245	21.803043842018237
40-41	22.159790130260006	27.363842774432655	28.623086107020253	21.853280988287086
42-43	22.073276786091277	27.443005364090478	28.428695985884193	22.055021863934048
44-45	22.178783860180054	27.561953350698154	28.212474033141703	22.04678875598009
46-47	22.268183782924762	27.53506190749072	27.917620954509342	22.279133355075174
48-49	22.195908055964537	27.625484942836685	27.89614861657535	22.28245838462343
50-51	22.197592657409135	27.77283758931435	27.77165661606521	22.257913137211315
52-53	22.41362146951334	27.67702051891468	27.601709396458023	22.307648615113955
54-55	22.39762253418397	27.577859602730964	27.6481316361403	22.376386226944767
56-57	22.372748014993032	27.720004731680483	27.62400442808864	22.28324282523785
58-59	22.269757082854007	27.75890874555231	27.667895074774606	22.30343909681907
60-61	22.37652833765299	27.738352626730713	27.626037760967343	22.259081274648963
62-63	22.34066468644019	27.849365792147484	27.57742351419435	22.232546007217977
64-65	22.343496537948834	27.721193612483713	27.576053959801172	22.35925588976628
66-67	22.401391240880763	27.805636344707434	27.396772784109853	22.39619963030195
68-69	22.349726485658408	27.752726930086887	27.50667656273788	22.390870021516825
70-71	22.343867376511923	27.567672583557695	27.563429777285144	22.52503026264524
72-73	22.390180132595724	27.570786742708552	27.47043825303244	22.56859487166328
74-75	22.31966616694388	27.72910178727067	27.392073192235415	22.559158853550034
76-77	22.462004141388128	27.621054045573008	27.395347156908045	22.52159465613082
78-79	22.407500635718055	27.60589681932371	27.356354528978827	22.630248015979404
80-81	22.385195992786848	27.663309500393417	27.299154436084283	22.652340070735445
82-83	22.47836401020673	27.585122082430935	27.382639326088615	22.553874581273725
84-85	22.510507818691618	27.561464894077663	27.387534001242535	22.540493285988177
86-87	22.43797805721464	27.56286273607959	27.459364536874542	22.539794669831224
88-89	22.48364506374366	27.5417429674221	27.410788448177676	22.563823520656562
90-91	22.501345586339	27.58153938227055	27.428376811423018	22.488738219967438
92-93	22.57114466573824	27.47452997129034	27.455867216375434	22.49845814659598
94-95	22.41051304728978	27.463201157280725	27.525156655661004	22.601129139768492
96-97	22.41364668059331	27.50700179876639	27.57672296029324	22.502628560347063
98-99	22.630831196712073	28.091414084557165	27.60295389243747	21.67480082629329
100-101	23.15786754088572	30.77974483444285	24.807984712649287	21.254402912022137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2145.0
1	1797.5
2	2109.5
3	2948.0
4	4037.5
5	6284.5
6	8200.5
7	10011.5
8	11426.0
9	11528.0
10	10453.0
11	9536.0
12	10889.5
13	14974.0
14	24244.0
15	36583.0
16	46145.5
17	51027.5
18	51282.0
19	47813.5
20	42548.5
21	37425.0
22	34596.5
23	36282.5
24	42431.0
25	53117.0
26	67309.5
27	83188.0
28	100407.5
29	119419.0
30	137073.5
31	153334.0
32	171577.0
33	191431.0
34	211028.0
35	228965.5
36	249102.0
37	265829.0
38	278008.0
39	290172.0
40	302350.5
41	312059.0
42	316569.0
43	322121.5
44	327900.5
45	332058.0
46	333513.0
47	331735.0
48	330029.0
49	327616.0
50	323339.5
51	315664.0
52	303021.0
53	287021.0
54	270247.5
55	253446.5
56	234158.0
57	211968.0
58	189987.0
59	166243.0
60	140126.5
61	115015.5
62	92024.5
63	71348.0
64	53067.5
65	39373.0
66	29951.0
67	23073.5
68	17693.0
69	13142.5
70	9696.0
71	6953.0
72	4813.0
73	3262.5
74	2172.0
75	1399.5
76	894.5
77	536.0
78	330.5
79	202.5
80	136.5
81	94.5
82	61.0
83	35.0
84	21.0
85	17.5
86	12.0
87	7.5
88	7.5
89	7.0
90	5.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4356969905258696E-5
2	0.005382890349062171
3	2.4356969905258696E-5
4	2.4356969905258696E-5
5	2.4356969905258696E-5
6	2.4356969905258696E-5
7	2.4356969905258696E-5
8	1.2178484952629348E-5
9	2.0703424419469892E-4
10-11	8.342262192551104E-4
12-13	0.0
14-15	1.2178484952629348E-5
16-17	0.001266562435073452
18-19	0.0
20-21	0.0
22-23	8.525626816262997E-4
24-25	0.002844264403793457
26-27	0.007633096717488186
28-29	0.3631670797092445
30-31	0.33404219603648483
32-33	0.33252606135038915
34-35	0.18651259143845064
36-37	0.049749483724517304
38-39	0.04793540657574814
40-41	0.027815994613738425
42-43	0.027187053125731383
44-45	0.0152630974047051
46-47	0.010419182722878052
48-49	0.010084828402171887
50-51	0.008953830546031837
52-53	0.007751437921145393
54-55	0.0012112316191487556
56-57	0.004212690028808166
58-59	0.0033640752742273112
60-61	0.006365947146049461
62-63	0.0018835415122689608
64-65	1.9887741244301903E-4
66-67	6.160673266062935E-4
68-69	0.0015727403966898639
70-71	0.003988502383006636
72-73	0.001981554719279273
74-75	0.001425017316119508
76-77	4.5793916038381407E-4
78-79	2.130073292883976E-4
80-81	0.0013589076283653477
82-83	0.001351468539501736
84-85	0.0
86-87	0.0037930240452386456
88-89	0.0019041632314007553
90-91	0.00489781249677646
92-93	0.013725662101506662
94-95	0.001539948118337394
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	93.0
20-21	402.0
22-23	814.0
24-25	1671.0
26-27	2674.0
28-29	5053.0
30-31	14532.0
32-33	20386.0
34-35	29373.0
36-37	40524.0
38-39	46657.0
40-41	51020.0
42-43	61892.0
44-45	75546.0
46-47	74705.0
48-49	64939.0
50-51	57478.0
52-53	53731.0
54-55	57838.0
56-57	60465.0
58-59	61453.0
60-61	61754.0
62-63	61299.0
64-65	66225.0
66-67	69984.0
68-69	70382.0
70-71	68953.0
72-73	67226.0
74-75	68296.0
76-77	70228.0
78-79	73670.0
80-81	74280.0
82-83	72905.0
84-85	73726.0
86-87	77517.0
88-89	82247.0
90-91	87660.0
92-93	89924.0
94-95	99710.0
96-97	150283.0
98-99	693847.0
100-101	5249840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.89672658270977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07287949610667	77.3696550259444
2	7.04652460865109	12.105467953352774
3	1.629171738313958	4.198215580866962
4	0.6167602418025269	2.1191074342879044
5	0.2502477892387603	1.0747732965084686
6	0.13596526308089665	0.700738261656359
7	0.07691253122482836	0.46245742657822464
8	0.04168513943160938	0.28644936194552684
9	0.031444344456111335	0.24308696324872275
>10	0.09612851816625452	1.2177188569254098
>50	0.0017675167542210682	0.09913591297981891
>100	4.469278196361084E-4	0.07137382034058469
>500	4.3923543975587515E-5	0.027806065519192985
>1k	2.196140947502092E-5	0.024014039845645808
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4356969905258693E-5	0.0	0.0	0.0
3	0.0	2.4356969905258693E-5	0.0	0.0	0.0
4	0.0	8.524939466840543E-5	0.0	0.0	0.0
5	0.0	8.524939466840543E-5	0.0	1.2178484952629346E-5	0.0
6	2.4356969905258693E-5	8.524939466840543E-5	0.0	1.2178484952629346E-5	1.2178484952629346E-5
7	2.4356969905258693E-5	9.742787962103477E-5	0.0	1.2178484952629346E-5	1.2178484952629346E-5
8	2.4356969905258693E-5	1.0960636457366413E-4	0.0	1.2178484952629346E-5	1.2178484952629346E-5
9	2.4356969905258693E-5	2.4356969905258693E-4	0.0	3.6535454857888044E-5	1.2178484952629346E-5
10-11	2.4356969905258693E-5	2.4356969905258693E-4	0.0	7.916015219209077E-5	1.2178484952629346E-5
12-13	2.4356969905258693E-5	2.4356969905258693E-4	0.0	9.133863714472009E-5	1.826772742894402E-5
14-15	2.4356969905258693E-5	3.409975786736217E-4	0.0	1.0960636457366413E-4	3.6535454857888044E-5
16-17	1.2178484952629347E-4	3.7753303353150975E-4	0.0	1.2178484952629347E-4	3.6535454857888044E-5
18-19	1.2178484952629347E-4	4.140684883893978E-4	0.0	1.4614181943155218E-4	6.089242476314673E-5
20-21	1.2178484952629347E-4	4.810501556288592E-4	0.0	1.8876651676575488E-4	6.089242476314673E-5
22-23	1.2787409200260816E-4	5.662995502972647E-4	0.0	4.384254582946565E-4	7.916015219209075E-5
24-25	1.4614181943155218E-4	6.211027325840967E-4	0.0	0.0014005257695523749	1.0960636457366413E-4
26-27	1.4614181943155218E-4	6.33281217536726E-4	0.0	0.003842312002554559	1.0960636457366413E-4
28-29	1.4614181943155218E-4	6.393704600130407E-4	0.0	0.018596546522665014	1.0960636457366413E-4
30-31	1.4614181943155218E-4	7.307090971577609E-4	0.0	0.02549565824832954	1.0960636457366413E-4
32-33	1.4614181943155218E-4	7.307090971577609E-4	0.0	0.027657339327421246	1.2178484952629347E-4
34-35	1.5223106190786684E-4	8.707616741129983E-4	0.0	0.030653246625768068	1.2178484952629347E-4
36-37	1.583203043841815E-4	0.0010229927360208653	0.0	0.035153196815764606	1.2178484952629347E-4
38-39	1.583203043841815E-4	0.0010412604634498094	0.0	0.04344065582602888	1.3396333447892282E-4
40-41	1.583203043841815E-4	0.0011691345554524174	0.0	0.06318197993424104	1.4614181943155218E-4
42-43	1.583203043841815E-4	0.0011995807678339908	0.0	0.07095794257649489	1.4614181943155218E-4
44-45	1.7049878933681086E-4	0.0013579010722181723	0.0	0.08200991767100602	1.4614181943155218E-4
46-47	1.826772742894402E-4	0.0014918644066970951	0.0	0.09559501763566405	1.4614181943155218E-4
48-49	1.826772742894402E-4	0.0014979536491734097	0.0	0.1071036859158988	1.4614181943155218E-4
50-51	1.826772742894402E-4	0.001638006226128647	0.0	0.11786946661402313	1.4614181943155218E-4
52-53	1.826772742894402E-4	0.0016440954686049618	0.0	0.13466968660617534	1.644095468604962E-4
54-55	1.826772742894402E-4	0.0016745416809865353	0.0	0.17385396194126024	1.8876651676575488E-4
56-57	1.826772742894402E-4	0.0017293448632733674	0.0	0.21485283153428694	1.9485575924206954E-4
58-59	1.826772742894402E-4	0.0017415233482259967	0.0	0.24364276996230272	1.9485575924206954E-4
60-61	2.0094500171838423E-4	0.0018024157729891433	0.0	0.2631161674015571	1.9485575924206954E-4
62-63	2.313912140999576E-4	0.0018389512278470315	0.0	0.2753616340214259	2.1921272914732825E-4
64-65	2.313912140999576E-4	0.0021312348667101356	0.0	0.2842580172793216	2.313912140999576E-4
66-67	2.313912140999576E-4	0.002186038048996968	0.0	0.28951303353638114	2.313912140999576E-4
68-69	2.313912140999576E-4	0.0022225735038548557	0.0	0.29488374540049067	2.313912140999576E-4
70-71	2.3748045657627227E-4	0.002277376686141688	0.0	0.30013267241507396	2.313912140999576E-4
72-73	2.6792666895784564E-4	0.002313912140999576	0.0	0.30455955169535476	2.313912140999576E-4
74-75	2.6792666895784564E-4	0.0023748045657627226	0.0	0.30829225733333565	2.313912140999576E-4
76-77	2.8010515391047497E-4	0.002460053960431128	0.0	0.3116535191802613	2.313912140999576E-4
78-79	2.8010515391047497E-4	0.0024965894152890163	0.0	0.3143936782946029	2.313912140999576E-4
80-81	2.8010515391047497E-4	0.0024965894152890163	0.0	0.31569677618453423	2.313912140999576E-4
82-83	2.8619439638678963E-4	0.0025148571427179604	0.0	0.31615346937025784	2.4356969905258693E-4
84-85	2.9228363886310435E-4	0.002533124870146904	0.0	0.316330057402071	2.4356969905258693E-4
86-87	2.9228363886310435E-4	0.0025818388099574214	0.0	0.31649446694893146	2.4356969905258693E-4
88-89	2.9228363886310435E-4	0.0027158021444363446	0.0	0.31651882391883673	2.4356969905258693E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10420	0.0	53.653133	1
GTATCAA	19540	0.0	38.500057	1
ATCAACG	24455	0.0	30.12869	3
TCAACGC	24400	0.0	30.108986	4
CAACGCA	25135	0.0	29.297401	5
TATCAAC	25295	0.0	29.231443	2
AACGCAG	25695	0.0	28.658892	6
ACGCAGA	29725	0.0	24.758986	7
CGCAGAG	30505	0.0	24.18221	8
GTGGTAT	5050	0.0	21.85212	1
TGGTATC	4765	0.0	20.996399	2
GCAGAGT	35080	0.0	20.955019	9
GTACATG	25185	0.0	19.879635	1
GAGTACT	20465	0.0	19.638363	12-13
TACATGG	25210	0.0	19.31488	2
ACATGGG	25010	0.0	18.987688	3
AGAGTAC	31745	0.0	18.96353	10-11
CAGAGTA	34060	0.0	18.859644	10-11
GTACTTT	22650	0.0	17.6588	14-15
AGTACTT	22400	0.0	17.414785	12-13
>>END_MODULE