FastQCFastQC Report
Fri 27 May 2016
SRR2139635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10659627
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144250.055.0710561
GTATCAA273500.038.6026761
TCAACGC343900.030.1894114
ATCAACG347600.029.843353
CAACGCA352250.029.4860385
TATCAAC355000.029.3433512
AACGCAG361000.028.806846
ACGCAGA414750.025.0632887
CGCAGAG424600.024.491928
TGGTATC60500.022.590242
GTGGTAT64300.022.2515411
GCAGAGT487200.021.344979
GTACATG341600.020.9672491
TACATGG345050.020.1634712
GAGTACT291150.020.06694612-13
ACATGGG345000.019.7566623
AGAGTAC445700.019.4279610-11
CAGAGTA479450.018.94222610-11
GTACTTT322300.018.10791614-15
CATGGGG244950.018.0278914
Produced by FastQC (version 0.11.5)