##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10659627
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91394792707099	34.0	31.0	34.0	31.0	34.0
2	33.04899327152817	34.0	33.0	34.0	31.0	34.0
3	33.10749353612467	34.0	33.0	34.0	31.0	34.0
4	36.471277559711986	37.0	37.0	37.0	35.0	37.0
5	36.436200159724166	37.0	37.0	37.0	35.0	37.0
6	36.464572259423335	37.0	37.0	37.0	35.0	37.0
7	36.43990816939467	37.0	37.0	37.0	35.0	37.0
8	36.42797745174386	37.0	37.0	37.0	35.0	37.0
9	38.218342255315314	39.0	39.0	39.0	37.0	39.0
10-11	38.158424211278685	39.0	39.0	39.0	37.0	39.0
12-13	38.12878504097751	39.0	39.0	39.0	37.0	39.0
14-15	39.63180634744536	41.0	40.0	41.0	37.0	41.0
16-17	39.60698057258476	41.0	40.0	41.0	37.0	41.0
18-19	39.61933335003185	41.0	40.0	41.0	37.0	41.0
20-21	39.61233693846937	41.0	40.0	41.0	37.0	41.0
22-23	39.553382138677314	41.0	40.0	41.0	37.0	41.0
24-25	39.487322999737	41.0	39.0	41.0	36.5	41.0
26-27	39.435801692483906	41.0	39.0	41.0	36.5	41.0
28-29	39.26940234174146	41.0	39.0	41.0	36.0	41.0
30-31	39.18385741528803	41.0	39.0	41.0	36.0	41.0
32-33	39.075928694569924	41.0	39.0	41.0	35.0	41.0
34-35	38.97858496739079	40.0	39.0	41.0	35.0	41.0
36-37	39.06968463599708	40.5	39.0	41.0	35.0	41.0
38-39	39.094067705354924	41.0	39.0	41.0	35.0	41.0
40-41	39.08685818242015	41.0	39.0	41.0	35.0	41.0
42-43	39.04456351743416	40.5	39.0	41.0	35.0	41.0
44-45	38.976351322941056	40.0	39.0	41.0	35.0	41.0
46-47	38.888460093056054	40.0	38.0	41.0	35.0	41.0
48-49	38.81186376393822	40.0	38.0	41.0	35.0	41.0
50-51	38.721721442301956	40.0	38.0	41.0	35.0	41.0
52-53	38.616727113174235	40.0	38.0	41.0	35.0	41.0
54-55	38.4958399521076	40.0	38.0	41.0	34.5	41.0
56-57	38.32380255954405	40.0	37.0	41.0	34.0	41.0
58-59	38.16372349537308	40.0	37.0	41.0	34.0	41.0
60-61	37.97530976566376	39.5	36.5	41.0	34.0	41.0
62-63	37.72699891294706	39.0	36.0	41.0	34.0	41.0
64-65	37.43141295159583	39.0	35.5	40.0	33.0	41.0
66-67	37.11043005940396	38.0	35.0	40.0	33.0	41.0
68-69	36.802071090467464	37.5	35.0	40.0	33.0	41.0
70-71	36.47894210337992	37.0	35.0	39.5	32.5	41.0
72-73	36.141519604013574	36.5	35.0	39.0	32.0	40.5
74-75	35.775023100410266	36.0	35.0	39.0	32.0	40.0
76-77	34.83992957355921	35.0	34.0	37.0	30.5	39.0
78-79	35.07724158257901	35.0	34.0	37.0	31.0	39.0
80-81	34.897384376518225	35.0	34.0	37.0	31.0	39.0
82-83	34.628692430981914	35.0	34.0	36.0	31.0	38.5
84-85	34.37585999972656	35.0	34.0	36.0	31.0	37.0
86-87	34.122480342221536	35.0	34.0	35.5	31.0	37.0
88-89	33.817830313462984	35.0	34.0	35.0	31.0	36.5
90-91	33.7368168463297	35.0	34.0	35.0	31.0	36.0
92-93	33.64739945446872	35.0	34.0	35.0	31.0	36.0
94-95	33.538888997711595	35.0	33.5	35.0	30.0	35.5
96-97	33.502870382665684	35.0	33.0	35.0	30.0	35.0
98-99	33.50445267347597	34.5	33.0	35.0	30.0	35.0
100-101	32.42731681601345	33.5	31.0	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	12.0
20	37.0
21	170.0
22	894.0
23	3141.0
24	8054.0
25	16720.0
26	29977.0
27	47293.0
28	67415.0
29	90839.0
30	117948.0
31	148694.0
32	189149.0
33	250042.0
34	366151.0
35	557262.0
36	1044931.0
37	2461934.0
38	3508684.0
39	1659920.0
40	90359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.37999126423221	21.163307448743033	15.359594297134683	24.097106989890076
2	13.70830815140268	21.66583889180867	41.423430012558285	23.202422944230367
3	16.784119214711513	26.80423812322086	31.342503262779246	25.06913939928838
4	11.900147697517285	18.715491278116374	39.310945676883165	30.073415347483174
5	12.957051768430105	37.990458200026566	35.70831391426189	13.344176117281433
6	29.24188507962382	38.567992642141974	18.864924316279826	13.32519796195438
7	25.91964782247479	33.12168421700428	23.367897404261164	17.59077055625977
8	24.430022216539303	36.45848362784961	21.264498397973814	17.846995757637277
9	25.46310111866246	16.880419114967204	21.51824575113243	36.1382340152379
10-11	23.83986641071435	27.06078476669368	29.07402877470059	20.025320047891384
12-13	24.840259419959065	24.693612637665463	29.133022196742907	21.333105745632565
14-15	22.03505489499442	25.989082729372072	26.961560745482206	25.01430163015131
16-17	21.40305290575041	29.16122393917004	27.565916886783636	21.869806268295914
18-19	21.426863381510756	28.19583445047697	29.49107220084457	20.886229967167704
20-21	22.297516206935615	27.28489237553418	29.429210502183402	20.988380915346802
22-23	22.046542990105138	27.149397363383887	29.27323335705739	21.530826289453593
24-25	21.76757151657013	27.367951029639393	29.334871066290123	21.52960638750035
26-27	21.658710069054802	27.51066488207593	29.27997414539909	21.55065090347018
28-29	21.59976017742799	27.5111820761088	29.324472739972883	21.564585006490326
30-31	21.8993100752403	27.40309566691037	29.1963294861672	21.50126477168213
32-33	21.707638574769273	27.581560021295005	29.074804545738264	21.635996858197455
34-35	21.837667851154574	27.561601202782143	29.122531112149964	21.478199833913322
36-37	21.822977074747673	27.495154698471335	29.081988576288108	21.59987965049288
38-39	21.667665408550974	27.679760653209645	29.10008520009614	21.552488738143243
40-41	21.901066313392658	27.52834597550333	28.95383578597065	21.61675192513336
42-43	21.810640368496237	27.650544424390006	28.69460194481976	21.844213262293998
44-45	21.920503869215953	27.756978548449435	28.509633384886463	21.81288419744815
46-47	22.030438757607804	27.71035789875998	28.20226794856449	22.056935395067722
48-49	21.94396399151771	27.849957822931753	28.163920823657858	22.04215736189268
50-51	22.00060822857268	27.963722714225277	27.992011014568725	22.043658042633318
52-53	22.16209263637257	27.85974490014492	27.872860066836264	22.10530239664624
54-55	22.135413415519118	27.786156484851755	27.91172413680647	22.16670596282266
56-57	22.088336433773925	27.931994394393815	27.868374185668777	22.111294986163475
58-59	22.04640180180731	27.937623498262937	27.923723670174226	22.092251029755527
60-61	22.156334028980133	27.93857036561354	27.879244341231086	22.025851264175238
62-63	22.12297486417401	28.026727162726132	27.84198916406833	22.008308809031526
64-65	22.119171549513872	27.929781434087133	27.814684895442987	22.136362120956008
66-67	22.162580581982386	28.035378600157134	27.599392721875404	22.20264809598508
68-69	22.095112690821118	27.974629312574873	27.719588337792082	22.210669658811923
70-71	22.102013211448433	27.76666513798239	27.797463468965983	22.3338581816032
72-73	22.150475696914548	27.799011979617084	27.692602376973685	22.35790994649468
74-75	22.04296383986838	27.934277011180125	27.64171448193464	22.38104466701685
76-77	22.19690581466187	27.80558196289546	27.673811642878032	22.32370057956463
78-79	22.176440311951037	27.825437851955925	27.573004630673054	22.42511720541998
80-81	22.165858733758586	27.865775361549016	27.527090194411986	22.44127571028042
82-83	22.231084194387453	27.805144640564816	27.64934342208225	22.31442774296548
84-85	22.27699270530883	27.762497685244664	27.609521522720637	22.35098808672587
86-87	22.180842819630282	27.772531383575465	27.698341345781703	22.34828445101255
88-89	22.25459047402531	27.75075110464755	27.647139610345	22.34751881098214
90-91	22.274775215554353	27.790847689155346	27.670859583671092	22.263517511619206
92-93	22.36193202568958	27.66429650244585	27.717021228518483	22.256750243346087
94-95	22.203793892810335	27.666976024924644	27.750959143344378	22.378270938920643
96-97	22.218758950359714	27.68913517362379	27.808775669277825	22.283330206738675
98-99	22.40914099710548	28.28019014410635	27.84762805936889	21.463040799419282
100-101	22.92464839075817	30.961941207096295	25.073207801231884	21.040202600913656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3377.0
1	2792.5
2	3146.5
3	4249.0
4	5606.0
5	8650.5
6	11282.5
7	13958.0
8	16373.5
9	16775.0
10	15440.0
11	14181.0
12	15765.0
13	21059.5
14	33542.5
15	50404.0
16	63562.5
17	70511.0
18	70879.0
19	66152.0
20	59260.0
21	52604.5
22	49185.0
23	51377.5
24	59520.5
25	73929.5
26	93155.5
27	114779.5
28	139213.5
29	165341.0
30	189590.0
31	213367.0
32	239980.5
33	268486.5
34	293975.5
35	316581.0
36	342934.5
37	364451.5
38	377376.0
39	389497.5
40	402427.5
41	412749.5
42	415839.0
43	420227.0
44	426660.0
45	430706.0
46	430043.0
47	424413.0
48	419746.0
49	416373.5
50	409310.5
51	396635.5
52	379468.0
53	358529.5
54	336532.0
55	314340.5
56	288970.0
57	260911.0
58	233242.5
59	203823.0
60	173167.0
61	142525.0
62	112796.0
63	86737.5
64	65103.5
65	48901.5
66	37490.5
67	28945.0
68	22338.5
69	16599.5
70	12030.0
71	8708.5
72	6105.5
73	4171.5
74	2768.5
75	1774.0
76	1122.5
77	695.5
78	444.5
79	273.5
80	173.5
81	110.5
82	76.0
83	55.0
84	38.5
85	27.5
86	22.0
87	19.5
88	13.0
89	8.5
90	6.5
91	4.5
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.8143573879273636E-5
2	0.004962650194045251
3	2.8143573879273636E-5
4	2.8143573879273636E-5
5	2.8143573879273636E-5
6	2.8143573879273636E-5
7	2.8143573879273636E-5
8	9.381191293091212E-6
9	2.0638620844800668E-4
10-11	8.396166207316635E-4
12-13	0.0
14-15	2.8143573879273636E-5
16-17	0.0013415103549120435
18-19	9.381191293091212E-6
20-21	4.690694216177022E-6
22-23	6.895752315084655E-4
24-25	0.0025898252989242513
26-27	0.007217816236088105
28-29	0.3643621010644853
30-31	0.33418248490706154
32-33	0.3331013353392628
34-35	0.18612542329411202
36-37	0.048334728668565585
38-39	0.04680186686545654
40-41	0.026616179286583674
42-43	0.02603240229183759
44-45	0.014357633432841875
46-47	0.009795453874671109
48-49	0.009573583914428481
50-51	0.008180652495869398
52-53	0.00700471789244678
54-55	8.764486179704681E-4
56-57	0.003960694295839925
58-59	0.002978815904618211
60-61	0.005977707644218336
62-63	0.0015864099584803311
64-65	1.5951633795578004E-4
66-67	7.086794006333231E-4
68-69	0.001643194385069209
70-71	0.004087282661073043
72-73	0.0021694314927293607
74-75	0.0014030372682473102
76-77	5.47816347562703E-4
78-79	1.5984348126314713E-4
80-81	0.0012066884721239663
82-83	0.0013665562228386001
84-85	5.906871321643556E-6
86-87	0.0038611757877979077
88-89	0.0016463291550669564
90-91	0.005349423811230122
92-93	0.013475047262250062
94-95	0.001491774821394731
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	126.0
20-21	526.0
22-23	1186.0
24-25	2775.0
26-27	3954.0
28-29	7131.0
30-31	20532.0
32-33	27921.0
34-35	41282.0
36-37	56302.0
38-39	64306.0
40-41	70855.0
42-43	85074.0
44-45	103623.0
46-47	102472.0
48-49	89079.0
50-51	78455.0
52-53	72503.0
54-55	77965.0
56-57	81342.0
58-59	82702.0
60-61	82744.0
62-63	81814.0
64-65	88552.0
66-67	93056.0
68-69	94115.0
70-71	92236.0
72-73	89462.0
74-75	91297.0
76-77	93364.0
78-79	98092.0
80-81	99010.0
82-83	96699.0
84-85	98409.0
86-87	103039.0
88-89	108673.0
90-91	116755.0
92-93	119226.0
94-95	131866.0
96-97	196253.0
98-99	889490.0
100-101	6725364.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.39626442707977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.45511580828739	75.49677608111266
2	7.3117109123422095	12.341621751447954
3	1.7766359278364257	4.498243066689996
4	0.6685912481354614	2.2570641508508684
5	0.2925241135970821	1.234397122121823
6	0.16823267723009427	0.8518925707671959
7	0.09690046407672351	0.5724626032528131
8	0.05749327078508983	0.3881773827164913
9	0.03703215643366068	0.2812838100012189
>10	0.13193009615158863	1.718886229862511
>50	0.003020556542962149	0.17167225537430042
>100	6.806412967551961E-4	0.09178398615407013
>500	9.437759886870267E-5	0.053445736302370345
>1k	3.774968574710909E-5	0.04229325334569482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.381191293091212E-6	0.0
3	0.0	0.0	0.0	9.381191293091212E-6	0.0
4	0.0	4.6905956465456064E-5	0.0	9.381191293091212E-6	0.0
5	0.0	4.6905956465456064E-5	0.0	9.381191293091212E-6	0.0
6	2.8143573879273636E-5	4.6905956465456064E-5	0.0	9.381191293091212E-6	0.0
7	2.8143573879273636E-5	4.6905956465456064E-5	0.0	9.381191293091212E-6	0.0
8	2.8143573879273636E-5	4.6905956465456064E-5	0.0	9.381191293091212E-6	0.0
9	2.8143573879273636E-5	9.381191293091213E-5	0.0	1.8762382586182425E-5	0.0
10-11	2.8143573879273636E-5	9.381191293091213E-5	0.0	4.6905956465456064E-5	1.8762382586182425E-5
12-13	2.8143573879273636E-5	9.381191293091213E-5	0.0	5.628714775854727E-5	2.345297823272803E-5
14-15	2.8143573879273636E-5	1.3602727374982258E-4	0.0	7.50495303447297E-5	3.752476517236485E-5
16-17	4.6905956465456064E-5	1.500990606894594E-4	0.0	8.912131728436652E-5	4.6905956465456064E-5
18-19	4.6905956465456064E-5	1.54789656336005E-4	0.0	1.2664608245673136E-4	5.628714775854727E-5
20-21	5.1596552112001664E-5	1.594802519825506E-4	0.0	1.7824263456873301E-4	7.97401259912753E-5
22-23	5.628714775854727E-5	1.594802519825506E-4	0.0	4.456065864218326E-4	8.912131728436652E-5
24-25	5.628714775854727E-5	1.594802519825506E-4	0.0	0.0013884163113774995	9.381191293091213E-5
26-27	5.628714775854727E-5	1.594802519825506E-4	0.0	0.004690595646545606	1.0319310422400333E-4
28-29	5.628714775854727E-5	1.594802519825506E-4	0.0	0.02039940046682684	1.0319310422400333E-4
30-31	5.628714775854727E-5	1.7824263456873304E-4	0.0	0.027988784222937633	1.0319310422400333E-4
32-33	5.628714775854727E-5	1.8293323021527865E-4	0.0	0.030502943489486074	1.0319310422400333E-4
34-35	5.628714775854727E-5	2.0638620844800666E-4	0.0	0.03412877392426583	1.2195548681018576E-4
36-37	5.628714775854727E-5	2.2514859103418909E-4	0.0	0.039199307818181636	1.2195548681018576E-4
38-39	5.628714775854727E-5	2.2514859103418909E-4	0.0	0.04800824644239428	1.3133667810327697E-4
40-41	6.566833905163849E-5	2.4391097362037151E-4	0.0	0.06916283280831495	1.3133667810327697E-4
42-43	7.50495303447297E-5	2.5798276056000834E-4	0.0	0.07757307080257123	1.3133667810327697E-4
44-45	8.912131728436651E-5	2.86126334439282E-4	0.0	0.08962790161419344	1.3133667810327697E-4
46-47	1.0319310422400333E-4	3.001981213789188E-4	0.0	0.10368092617124408	1.3133667810327697E-4
48-49	1.0319310422400333E-4	3.048887170254644E-4	0.0	0.11623296012140012	1.4071786939636819E-4
50-51	1.1257429551709454E-4	3.0957931267201E-4	0.0	0.12771084766849722	1.500990606894594E-4
52-53	1.1257429551709454E-4	3.0957931267201E-4	0.0	0.1453474872995087	1.594802519825506E-4
54-55	1.1257429551709454E-4	3.0957931267201E-4	0.0	0.18720167225363515	1.6886144327564183E-4
56-57	1.1257429551709454E-4	3.142699083185556E-4	0.0	0.23039736756267362	1.8293323021527865E-4
58-59	1.1257429551709454E-4	3.189605039651012E-4	0.0	0.2619228609031067	2.0169561280146105E-4
60-61	1.1257429551709454E-4	3.2834169525819244E-4	0.0	0.2827631773607088	2.0638620844800666E-4
62-63	1.2195548681018576E-4	3.4241348219782926E-4	0.0	0.29488836710702915	2.1107680409455226E-4
64-65	1.2664608245673136E-4	4.0339122560292215E-4	0.0	0.30389900134404324	2.1576739974109787E-4
66-67	1.4071786939636819E-4	4.127724168960133E-4	0.0	0.31003899104537147	2.2514859103418909E-4
68-69	1.4071786939636819E-4	4.2215360818910453E-4	0.0	0.31584594845579494	2.4391097362037151E-4
70-71	1.4071786939636819E-4	4.3622539512874135E-4	0.0	0.3211650839189777	2.5329216491346273E-4
72-73	1.7355203892218743E-4	4.4091599077528696E-4	0.0	0.32541945417039453	2.6267335620655394E-4
74-75	1.8762382586182426E-4	4.4560658642183256E-4	0.0	0.3288951855444848	2.6736395185309955E-4
76-77	1.8762382586182426E-4	4.549877777149238E-4	0.0	0.3319393821190929	2.7674514314619076E-4
78-79	1.8762382586182426E-4	4.596783733614694E-4	0.0	0.3350117222675803	2.8143573879273637E-4
80-81	1.8762382586182426E-4	4.596783733614694E-4	0.0	0.3362312771356821	2.86126334439282E-4
82-83	1.8762382586182426E-4	4.596783733614694E-4	0.0	0.33671440848727635	2.908169300858276E-4
84-85	1.8762382586182426E-4	4.596783733614694E-4	0.0	0.3370333689912414	2.955075257323732E-4
86-87	1.8762382586182426E-4	4.596783733614694E-4	0.0	0.33717877745628433	3.048887170254644E-4
88-89	1.8762382586182426E-4	5.441090949992903E-4	0.0	0.33720692103016364	3.142699083185556E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14425	0.0	55.071056	1
GTATCAA	27350	0.0	38.602676	1
TCAACGC	34390	0.0	30.189411	4
ATCAACG	34760	0.0	29.84335	3
CAACGCA	35225	0.0	29.486038	5
TATCAAC	35500	0.0	29.343351	2
AACGCAG	36100	0.0	28.80684	6
ACGCAGA	41475	0.0	25.063288	7
CGCAGAG	42460	0.0	24.49192	8
TGGTATC	6050	0.0	22.59024	2
GTGGTAT	6430	0.0	22.251541	1
GCAGAGT	48720	0.0	21.34497	9
GTACATG	34160	0.0	20.967249	1
TACATGG	34505	0.0	20.163471	2
GAGTACT	29115	0.0	20.066946	12-13
ACATGGG	34500	0.0	19.756662	3
AGAGTAC	44570	0.0	19.42796	10-11
CAGAGTA	47945	0.0	18.942226	10-11
GTACTTT	32230	0.0	18.107916	14-15
CATGGGG	24495	0.0	18.027891	4
>>END_MODULE