FastQCFastQC Report
Fri 27 May 2016
SRR2139634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601866
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145300.053.1149441
GTATCAA261050.035.773861
TCAACGC327300.027.7758184
ATCAACG328100.027.7340983
CAACGCA335950.027.0482065
TATCAAC340300.026.8660582
AACGCAG346050.026.283426
TGGTATC63950.025.2170932
GTGGTAT69250.024.0263941
ACGCAGA397100.022.872277
CGCAGAG404950.022.4499598
GTACATG316350.019.891521
TACATGG312900.019.497292
GCAGAGT467950.019.2998929
GAGTACT279350.019.26513712-13
ACATGGG316250.018.7370473
AGAGTAC423500.018.40935110-11
CAGAGTA459850.018.03029810-11
GTACTTT315350.016.9985714-15
CATGGGG217150.016.9150284
Produced by FastQC (version 0.11.5)