##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10601866
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.862327160143316	34.0	31.0	34.0	31.0	34.0
2	33.01110172492276	34.0	31.0	34.0	31.0	34.0
3	33.068364380383606	34.0	33.0	34.0	31.0	34.0
4	36.45068858632999	37.0	37.0	37.0	35.0	37.0
5	36.41156075732329	37.0	37.0	37.0	35.0	37.0
6	36.439268426897684	37.0	37.0	37.0	35.0	37.0
7	36.41452400926403	37.0	37.0	37.0	35.0	37.0
8	36.399980626052056	37.0	37.0	37.0	35.0	37.0
9	38.1817054658114	39.0	39.0	39.0	37.0	39.0
10-11	38.1165741955237	39.0	39.0	39.0	37.0	39.0
12-13	38.09065144758479	39.0	39.0	39.0	37.0	39.0
14-15	39.58422904043496	41.0	40.0	41.0	37.0	41.0
16-17	39.55357005078163	41.0	40.0	41.0	37.0	41.0
18-19	39.55874399846216	41.0	40.0	41.0	37.0	41.0
20-21	39.55006249416563	41.0	40.0	41.0	37.0	41.0
22-23	39.48989612407043	41.0	39.0	41.0	37.0	41.0
24-25	39.421275495730725	41.0	39.0	41.0	36.0	41.0
26-27	39.37089676477822	41.0	39.0	41.0	36.0	41.0
28-29	39.20458319800069	41.0	39.0	41.0	36.0	41.0
30-31	39.11948902673127	40.5	39.0	41.0	36.0	41.0
32-33	39.01436478176669	40.0	39.0	41.0	35.0	41.0
34-35	38.91583450199566	40.0	38.5	41.0	35.0	41.0
36-37	39.00977243342639	40.0	39.0	41.0	35.0	41.0
38-39	39.03904881517116	40.0	39.0	41.0	35.0	41.0
40-41	39.034604060224694	40.0	39.0	41.0	35.0	41.0
42-43	38.99294658587661	40.0	39.0	41.0	35.0	41.0
44-45	38.92444252803051	40.0	38.5	41.0	35.0	41.0
46-47	38.83998340322786	40.0	38.0	41.0	35.0	41.0
48-49	38.76705360798425	40.0	38.0	41.0	35.0	41.0
50-51	38.680079806541045	40.0	38.0	41.0	35.0	41.0
52-53	38.57584168159242	40.0	38.0	41.0	35.0	41.0
54-55	38.4574645930409	40.0	38.0	41.0	34.0	41.0
56-57	38.29030222962406	40.0	37.5	41.0	34.0	41.0
58-59	38.13509587702488	40.0	37.0	41.0	34.0	41.0
60-61	37.94795276383237	39.5	36.5	41.0	34.0	41.0
62-63	37.70254304630181	39.0	36.0	41.0	33.5	41.0
64-65	37.408274446108564	39.0	35.5	40.0	33.0	41.0
66-67	37.08322798078808	38.0	35.0	40.0	33.0	41.0
68-69	36.7761556747524	37.5	35.0	40.0	32.5	41.0
70-71	36.45032334742737	37.0	35.0	39.5	32.0	41.0
72-73	36.10717056899348	36.5	35.0	39.0	32.0	40.5
74-75	35.73924736819296	36.0	35.0	39.0	32.0	40.0
76-77	34.801716684662416	35.0	34.0	37.0	30.5	39.0
78-79	35.04361357104608	35.0	34.0	37.0	31.0	39.0
80-81	34.86237105489117	35.0	34.0	37.0	31.0	39.0
82-83	34.58849779038548	35.0	34.0	36.0	31.0	38.5
84-85	34.332142158531965	35.0	34.0	36.0	31.0	37.0
86-87	34.069991089824725	35.0	34.0	35.5	31.0	37.0
88-89	33.755190136517854	35.0	34.0	35.0	30.0	36.5
90-91	33.67080095155474	35.0	34.0	35.0	30.0	36.0
92-93	33.58238363522038	35.0	33.5	35.0	30.0	36.0
94-95	33.469492960611355	35.0	33.0	35.0	30.0	35.5
96-97	33.43276212241567	35.0	33.0	35.0	30.0	35.0
98-99	33.43512048615107	34.0	33.0	35.0	30.0	35.0
100-101	32.35862833214483	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.0
20	32.0
21	185.0
22	985.0
23	3309.0
24	8224.0
25	17303.0
26	30684.0
27	48472.0
28	70006.0
29	93803.0
30	121427.0
31	153812.0
32	195698.0
33	257959.0
34	371707.0
35	568083.0
36	1066032.0
37	2473687.0
38	3438573.0
39	1602756.0
40	79120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.65216167155496	21.58250458763623	15.413061758473626	24.352271982335182
2	13.626256867517702	21.990488763890205	41.28805786731433	23.095196501277762
3	16.66103086579578	26.824148392759202	31.500250191829455	25.014570549615563
4	11.849040116817203	18.95555526420215	39.1898763453178	30.005528273662847
5	12.899334220913019	37.915083808367676	35.89247740845597	13.29310456226334
6	29.183186165830254	38.5767504113663	18.94500637387856	13.295057048924882
7	25.68120797614382	32.98442302368498	23.623551139351427	17.710817860819773
8	24.091372216928605	36.71651763944197	21.447601771235366	17.744508372394066
9	25.117656452308612	16.923258894375042	21.71927498252426	36.239809670792084
10-11	23.60645675993521	27.145949343639014	29.32298306662989	19.92461082979588
12-13	24.587681074256174	24.820126004233593	29.298578193687792	21.29361472782244
14-15	21.814964716819386	26.232055691713473	27.052925454763173	24.90005413670397
16-17	21.26622316238577	29.250073679210832	27.741952553559447	21.741750604843947
18-19	21.314282911544336	28.31970129783769	29.593906355155436	20.772109435462536
20-21	22.09623158582519	27.44200476127076	29.542526273084825	20.91923737981923
22-23	21.836827361739587	27.361914102232305	29.378209301539105	21.423049234489003
24-25	21.61203462209736	27.52531181498249	29.45490326002468	21.40775030289547
26-27	21.559952812356826	27.66671695497661	29.375341327774656	21.397988904891907
28-29	21.497381314875483	27.64052447803258	29.436833217460073	21.425260989631862
30-31	21.718468578046757	27.58820114625143	29.314776620765205	21.37855365493661
32-33	21.556353984450947	27.7430821672557	29.209288293021274	21.491275555272086
34-35	21.675738198102252	27.71257674896468	29.237455756199132	21.37422929673394
36-37	21.66951239531386	27.640952643824324	29.18752803320134	21.502006927660478
38-39	21.55336752943026	27.794575666588678	29.207429076350284	21.444627727630774
40-41	21.714392849564355	27.681988835326777	29.097361374424313	21.506256940684555
42-43	21.67689333899922	27.791666713351937	28.86198539902339	21.669454548625453
44-45	21.764344315876038	27.8737879500099	28.66975836361431	21.692109370499754
46-47	21.854640472446327	27.877976528660575	28.34472347793473	21.92265952095837
48-49	21.82406538110092	27.987080438924387	28.288851867607605	21.900002312367086
50-51	21.842681837396924	28.096185420096454	28.160065872161976	21.901066870344643
52-53	21.99695203893214	28.01704744760036	28.03749842792651	21.948502085540994
54-55	21.96915409561661	27.95079046779197	28.063608000898594	22.016447435692825
56-57	21.966248063295836	28.042144796307888	28.02533317321277	21.966273967183504
58-59	21.900573658328177	28.092515645476528	28.02186716717003	21.985043529025265
60-61	22.00460810666846	28.099166278387987	27.974530535098186	21.921695079845367
62-63	21.965259520435666	28.176158056308715	27.963421525301996	21.895160897953623
64-65	21.96212410509644	28.083048252124843	27.951029087457226	22.00379855532149
66-67	22.045098971771875	28.155238030176317	27.745451765776767	22.05421123227504
68-69	21.967976111247328	28.13235030123647	27.804421783600127	22.095251803916074
70-71	21.960601814868184	27.93954642397637	27.9306703431891	22.16918141796635
72-73	22.022407646612756	27.95220732950107	27.830226256315672	22.195158767570497
74-75	21.933716029507444	28.06432026850803	27.795879950094882	22.20608375188964
76-77	22.08516497127733	27.95149771982064	27.781431408839946	22.181905900062084
78-79	22.04058100242363	27.95893489420555	27.707206904512798	22.29327719885802
80-81	22.01735641118058	27.998694912021964	27.697933680641846	22.286014996155608
82-83	22.103030983263956	27.9429667809252	27.783970023817684	22.17003221199316
84-85	22.122564737159188	27.919695388489725	27.750875456446916	22.206864417904175
86-87	22.04337856301679	27.8930703498953	27.85631253623606	22.207238550851848
88-89	22.174486626201823	27.883022477166758	27.747256774237684	22.195234122393735
90-91	22.13147746521021	27.931622496693336	27.800699206759255	22.1362008313372
92-93	22.210722550340098	27.820121793229397	27.824280016514802	22.144875639915703
94-95	22.06410399939526	27.789575883765533	27.916015601950594	22.23030451488861
96-97	22.055578156925222	27.833592157797316	27.949905696551664	22.160923988725795
98-99	22.28453234256893	28.38184662481541	28.030815290317797	21.302805742297863
100-101	22.778423323477636	31.12007585386369	25.246595647160554	20.854905175498125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4439.0
1	3639.5
2	3745.5
3	4820.0
4	6214.5
5	9166.5
6	11641.5
7	14077.0
8	15946.5
9	15984.0
10	14752.0
11	13650.5
12	15326.5
13	20968.5
14	34035.0
15	51176.0
16	64118.5
17	70287.5
18	70340.5
19	65958.0
20	59470.0
21	53349.5
22	50281.0
23	53648.5
24	63539.0
25	79324.5
26	99459.0
27	121350.0
28	144770.5
29	168952.0
30	191171.0
31	214001.0
32	240149.0
33	267857.0
34	293921.0
35	316673.0
36	341965.5
37	363027.0
38	377546.0
39	392326.5
40	406301.0
41	418627.0
42	425422.0
43	430563.5
44	435393.5
45	439468.5
46	440177.0
47	435396.0
48	429312.5
49	421923.0
50	412992.0
51	398414.0
52	377960.5
53	355564.0
54	330396.0
55	304451.5
56	278719.5
57	250512.0
58	221349.5
59	190682.0
60	158881.0
61	128984.0
62	101992.5
63	78146.5
64	58057.0
65	43153.5
66	32497.5
67	24895.0
68	19057.5
69	13931.5
70	9939.5
71	7072.0
72	4878.0
73	3210.0
74	2111.0
75	1351.0
76	861.0
77	545.5
78	358.0
79	240.5
80	158.0
81	100.0
82	66.5
83	41.0
84	29.5
85	25.5
86	21.0
87	18.0
88	14.0
89	9.0
90	5.5
91	2.5
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.43230182309416E-6
2	0.004810473929778022
3	9.43230182309416E-6
4	2.8296905469282484E-5
5	9.43230182309416E-6
6	9.43230182309416E-6
7	9.43230182309416E-6
8	0.0
9	2.35807545577354E-4
10-11	7.357195422013446E-4
12-13	0.0
14-15	1.4148452734641242E-5
16-17	0.0015516136498989895
18-19	9.43230182309416E-6
20-21	9.432554055019616E-6
22-23	7.49942763802272E-4
24-25	0.0026700036365826917
26-27	0.007318560567954275
28-29	0.36643658757875036
30-31	0.33726564163696615
32-33	0.33538479917008546
34-35	0.18774593867362
36-37	0.047662669746344755
38-39	0.046266278208597376
40-41	0.025923221046754718
42-43	0.025607821713845107
44-45	0.013892618950328358
46-47	0.009519575290877153
48-49	0.009328853677983319
50-51	0.007903467993180957
52-53	0.006853923611185481
54-55	7.039484985112516E-4
56-57	0.00376110302479717
58-59	0.002816453943401294
60-61	0.00592991442052929
62-63	0.0014994701606235626
64-65	9.1203013347561E-5
66-67	7.151068876224153E-4
68-69	0.0016911427193651548
70-71	0.0042636551436271136
72-73	0.0021563397730055755
74-75	0.0015066107200600476
76-77	5.929394706293419E-4
78-79	1.7865687719189656E-4
80-81	0.0011889727671615979
82-83	0.001320436130622258
84-85	0.0
86-87	0.003932581558846378
88-89	0.0016184861657604671
90-91	0.004915030634135519
92-93	0.014068141682013396
94-95	0.001403377610960124
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	158.0
20-21	655.0
22-23	1233.0
24-25	2666.0
26-27	3975.0
28-29	8113.0
30-31	22643.0
32-33	30163.0
34-35	44437.0
36-37	60223.0
38-39	67844.0
40-41	73313.0
42-43	86497.0
44-45	104676.0
46-47	102906.0
48-49	90172.0
50-51	79621.0
52-53	72585.0
54-55	78071.0
56-57	82318.0
58-59	83055.0
60-61	83173.0
62-63	81997.0
64-65	88639.0
66-67	93349.0
68-69	94445.0
70-71	92237.0
72-73	89487.0
74-75	90612.0
76-77	92686.0
78-79	96595.0
80-81	97536.0
82-83	94561.0
84-85	96427.0
86-87	101747.0
88-89	107409.0
90-91	115731.0
92-93	118779.0
94-95	131930.0
96-97	197923.0
98-99	884328.0
100-101	6656951.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.5461921781808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.91578695654314	76.91953190836801
2	7.112158323113424	12.168361254214181
3	1.6607910745179815	4.262230572855679
4	0.6315303983615201	2.161000832983907
5	0.26781743872153063	1.1455381040770112
6	0.14461322958882178	0.7422666677947638
7	0.08389467669303408	0.5023809094576047
8	0.04639642120234843	0.3175229731644737
9	0.03263656290190634	0.2512740313837589
>10	0.10191637501051384	1.3206244151733018
>50	0.002118068336942265	0.12023914068661334
>100	2.701637753721728E-4	0.04291077671206167
>500	7.031123345632578E-5	0.04611841312861808
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.886460364618832E-5	0.0	0.0	0.0
3	0.0	1.886460364618832E-5	0.0	9.43230182309416E-6	0.0
4	0.0	3.772920729237664E-5	0.0	1.886460364618832E-5	0.0
5	0.0	4.716150911547081E-5	0.0	2.8296905469282484E-5	0.0
6	9.43230182309416E-6	4.716150911547081E-5	0.0	2.8296905469282484E-5	0.0
7	9.43230182309416E-6	4.716150911547081E-5	0.0	2.8296905469282484E-5	0.0
8	1.886460364618832E-5	7.545841458475328E-5	0.0	3.772920729237664E-5	0.0
9	1.886460364618832E-5	1.414845273464124E-4	0.0	5.659381093856497E-5	0.0
10-11	2.35807545577354E-5	1.4620067825795948E-4	0.0	7.545841458475328E-5	0.0
12-13	3.772920729237664E-5	1.5091682916950656E-4	0.0	1.0375532005403578E-4	0.0
14-15	4.716150911547081E-5	2.2637524375425985E-4	0.0	1.3205222552331824E-4	1.886460364618832E-5
16-17	9.90391691424887E-5	2.641044510466365E-4	0.0	1.7921373463878904E-4	3.772920729237664E-5
18-19	1.0847147096558286E-4	2.9240135651591897E-4	0.0	2.546721492235423E-4	4.244535820392372E-5
20-21	1.1318762187712994E-4	3.2541441289674853E-4	0.0	3.6785977110067225E-4	4.716150911547081E-5
22-23	1.17903772788677E-4	3.678597711006723E-4	0.0	9.809593896017927E-4	4.716150911547081E-5
24-25	1.414845273464124E-4	4.0558897839304894E-4	0.0	0.002815542094193607	4.716150911547081E-5
26-27	1.5091682916950656E-4	4.338858838623314E-4	0.0	0.006861999576301002	4.716150911547081E-5
28-29	1.5091682916950656E-4	4.338858838623314E-4	0.0	0.026580226537479348	4.716150911547081E-5
30-31	1.6034913099260072E-4	4.857635438893493E-4	0.0	0.036238903604327764	5.187766002701789E-5
32-33	1.6034913099260072E-4	4.904796948008963E-4	0.0	0.03915348486766386	5.659381093856497E-5
34-35	1.6034913099260072E-4	5.470735057394613E-4	0.0	0.04318579389703662	5.659381093856497E-5
36-37	1.6034913099260072E-4	6.083834675895733E-4	0.0	0.04891120110365477	5.659381093856497E-5
38-39	1.6034913099260072E-4	6.225319203242146E-4	0.0	0.0594423660891394	5.659381093856497E-5
40-41	1.6034913099260072E-4	6.838418821743266E-4	0.0	0.08372111098178378	5.659381093856497E-5
42-43	1.6034913099260072E-4	7.121387876436092E-4	0.0	0.09357315023600563	5.659381093856497E-5
44-45	1.650652819041478E-4	8.441910131669274E-4	0.0	0.10743391776504249	5.659381093856497E-5
46-47	1.7449758372724196E-4	9.479463332209633E-4	0.0	0.12398760746457274	5.659381093856497E-5
48-49	1.7921373463878907E-4	9.526624841325103E-4	0.0	0.13814077635012553	5.659381093856497E-5
50-51	1.7921373463878907E-4	0.001056417804186546	0.0	0.15126110818604951	5.659381093856497E-5
52-53	1.7921373463878907E-4	0.0010752824078327345	0.0	0.17088029597808538	5.659381093856497E-5
54-55	1.8864603646188323E-4	0.0010988631623904698	0.0	0.21468390564453466	5.659381093856497E-5
56-57	1.8864603646188323E-4	0.0011035793133020169	0.0	0.2597797406607478	6.602611276165912E-5
58-59	1.8864603646188323E-4	0.0011082954642135639	0.0	0.2935190842819556	7.545841458475328E-5
60-61	1.980783382849774E-4	0.001117727766036658	0.0	0.3156755612644038	7.545841458475328E-5
62-63	1.980783382849774E-4	0.0011318762187712992	0.0	0.3294655865297675	7.545841458475328E-5
64-65	1.980783382849774E-4	0.0012544961424715234	0.0	0.3386149192981688	7.545841458475328E-5
66-67	2.0279448919652447E-4	0.0012827930479408058	0.0	0.34449124333395653	7.545841458475328E-5
68-69	2.0751064010807155E-4	0.0013016576515869943	0.0	0.34986765537312015	8.017456549630036E-5
70-71	2.0751064010807155E-4	0.0013158061043216354	0.0	0.3553478227323379	8.489071640784744E-5
72-73	2.0751064010807155E-4	0.0013252384061447297	0.0	0.35938956406353373	9.432301823094162E-5
74-75	2.0751064010807155E-4	0.001353535311614012	0.0	0.36298327105813255	9.432301823094162E-5
76-77	2.0751064010807155E-4	0.0014195614243756713	0.0	0.36616667292342686	9.432301823094162E-5
78-79	2.0751064010807155E-4	0.001462006782579595	0.0	0.36896806656488584	9.432301823094162E-5
80-81	2.0751064010807155E-4	0.001462006782579595	0.0	0.3701046589345687	9.90391691424887E-5
82-83	2.0751064010807155E-4	0.0014667229334911422	0.0	0.3706234355348389	1.0375532005403578E-4
84-85	2.0751064010807155E-4	0.0014714390844026892	0.0	0.37098186300411645	1.1318762187712992E-4
86-87	2.0751064010807155E-4	0.0014950198389604246	0.0	0.37108090217325895	1.2261992370022408E-4
88-89	2.0751064010807155E-4	0.0015468974989874425	0.0	0.37109505062599357	1.2261992370022408E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14530	0.0	53.114944	1
GTATCAA	26105	0.0	35.77386	1
TCAACGC	32730	0.0	27.775818	4
ATCAACG	32810	0.0	27.734098	3
CAACGCA	33595	0.0	27.048206	5
TATCAAC	34030	0.0	26.866058	2
AACGCAG	34605	0.0	26.28342	6
TGGTATC	6395	0.0	25.217093	2
GTGGTAT	6925	0.0	24.026394	1
ACGCAGA	39710	0.0	22.87227	7
CGCAGAG	40495	0.0	22.449959	8
GTACATG	31635	0.0	19.89152	1
TACATGG	31290	0.0	19.49729	2
GCAGAGT	46795	0.0	19.299892	9
GAGTACT	27935	0.0	19.265137	12-13
ACATGGG	31625	0.0	18.737047	3
AGAGTAC	42350	0.0	18.409351	10-11
CAGAGTA	45985	0.0	18.030298	10-11
GTACTTT	31535	0.0	16.99857	14-15
CATGGGG	21715	0.0	16.915028	4
>>END_MODULE