FastQCFastQC Report
Fri 27 May 2016
SRR2139633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9389857
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124150.053.0327451
GTATCAA237150.038.8142361
TCAACGC299300.030.110654
ATCAACG301050.030.0066243
TATCAAC307100.029.5417142
CAACGCA307700.029.2889635
AACGCAG315250.028.6281996
ACGCAGA361600.024.9231597
CGCAGAG372150.024.2164888
GAGACCG72200.023.8563688-89
CGAGACC71950.023.65902588-89
GACCGAG75750.023.14470190-91
AGACCGA74200.023.03550190-91
ACCGAGG77600.022.80041792-93
ATCTCCG75050.022.0791274-75
ACGAGAC81350.021.37269686-87
GCAGAGT422850.021.2320299
GCCCACG80250.021.11671482-83
CCCACGA79700.020.99876884-85
GTGGTAT60750.020.9720131
Produced by FastQC (version 0.11.5)