##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9389857
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.902735259972545	34.0	31.0	34.0	31.0	34.0
2	33.03850282277994	34.0	33.0	34.0	31.0	34.0
3	33.09742874678496	34.0	33.0	34.0	31.0	34.0
4	36.46263462798209	37.0	37.0	37.0	35.0	37.0
5	36.42628497963281	37.0	37.0	37.0	35.0	37.0
6	36.45524729503335	37.0	37.0	37.0	35.0	37.0
7	36.429744563735106	37.0	37.0	37.0	35.0	37.0
8	36.417099216739935	37.0	37.0	37.0	35.0	37.0
9	38.207127222491245	39.0	39.0	39.0	37.0	39.0
10-11	38.147863220920186	39.0	39.0	39.0	37.0	39.0
12-13	38.119839205218994	39.0	39.0	39.0	37.0	39.0
14-15	39.62064448904813	41.0	40.0	41.0	37.0	41.0
16-17	39.594124117119144	41.0	40.0	41.0	37.0	41.0
18-19	39.606435699712996	41.0	40.0	41.0	37.0	41.0
20-21	39.6024273166487	41.0	40.0	41.0	37.0	41.0
22-23	39.544214952672235	41.0	40.0	41.0	37.0	41.0
24-25	39.47933058530219	41.0	39.0	41.0	36.5	41.0
26-27	39.42960447838708	41.0	39.0	41.0	36.5	41.0
28-29	39.257811917546796	41.0	39.0	41.0	36.0	41.0
30-31	39.17084686435623	41.0	39.0	41.0	36.0	41.0
32-33	39.063727030937144	41.0	39.0	41.0	35.0	41.0
34-35	38.96731403947019	40.0	39.0	41.0	35.0	41.0
36-37	39.06229027381299	40.5	39.0	41.0	35.0	41.0
38-39	39.08846539271235	41.0	39.0	41.0	35.0	41.0
40-41	39.08226866328522	41.0	39.0	41.0	35.0	41.0
42-43	39.03984651633894	40.5	39.0	41.0	35.0	41.0
44-45	38.97391830891164	40.0	39.0	41.0	35.0	41.0
46-47	38.88898437156497	40.0	38.0	41.0	35.0	41.0
48-49	38.81274863252041	40.0	38.0	41.0	35.0	41.0
50-51	38.723575480782515	40.0	38.0	41.0	35.0	41.0
52-53	38.61838901121657	40.0	38.0	41.0	35.0	41.0
54-55	38.498661990549635	40.0	38.0	41.0	34.5	41.0
56-57	38.33048224697093	40.0	37.5	41.0	34.0	41.0
58-59	38.1722595456184	40.0	37.0	41.0	34.0	41.0
60-61	37.985211059289824	39.5	36.5	41.0	34.0	41.0
62-63	37.73500439895042	39.0	36.0	41.0	34.0	41.0
64-65	37.43905716001426	39.0	35.5	40.0	33.0	41.0
66-67	37.11979277253621	38.0	35.0	40.0	33.0	41.0
68-69	36.8124314086762	37.5	35.0	40.0	33.0	41.0
70-71	36.485442445443226	37.0	35.0	39.5	32.5	41.0
72-73	36.14411526898934	36.5	35.0	39.0	32.0	40.5
74-75	35.779421175030095	36.0	35.0	39.0	32.0	40.0
76-77	34.838426247603756	35.0	34.0	37.0	30.5	39.0
78-79	35.074681656707185	35.0	34.0	37.0	31.0	39.0
80-81	34.889934896151644	35.0	34.0	37.0	31.0	39.0
82-83	34.61397627083288	35.0	34.0	36.0	31.0	38.5
84-85	34.36409595623836	35.0	34.0	36.0	31.0	37.0
86-87	34.116750401088716	35.0	34.0	35.5	31.0	37.0
88-89	33.81822439420668	35.0	34.0	35.0	31.0	36.5
90-91	33.73351487273341	35.0	34.0	35.0	31.0	36.0
92-93	33.64817566787077	35.0	34.0	35.0	31.0	36.0
94-95	33.53893314263101	35.0	33.5	35.0	30.0	35.5
96-97	33.499768609070486	35.0	33.0	35.0	30.0	35.0
98-99	33.50810791900816	34.5	33.0	35.0	30.0	35.0
100-101	32.450338806475585	33.5	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	28.0
21	178.0
22	824.0
23	2725.0
24	7149.0
25	15072.0
26	26528.0
27	41764.0
28	60112.0
29	80818.0
30	104273.0
31	132222.0
32	167748.0
33	221221.0
34	321508.0
35	490924.0
36	920903.0
37	2176363.0
38	3111714.0
39	1435560.0
40	72220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.36132134095787	21.180422913878864	15.39256995981301	24.06568578535025
2	13.788498460333171	21.682958673793735	41.27915305064696	23.249389815226134
3	16.747468411386542	26.766217369703792	31.428227592438862	25.058086626470804
4	11.921387497464018	18.795689603300584	39.25219292523687	30.030729973998533
5	12.94887940229109	37.941427210537334	35.77167059555233	13.338022791619252
6	29.313977691881295	38.497122692522936	18.916873583244893	13.272026032350873
7	25.867992636734005	33.15017111552841	23.445260869310548	17.536575378427035
8	24.43944566993938	36.31560097241098	21.365373295887256	17.87958006176239
9	25.45488818493616	16.820764156132668	21.58064545330136	36.143702205629815
10-11	23.79100112430833	27.00754853179092	29.17451785043153	20.026932493469225
12-13	24.76268807927533	24.706558363987867	29.164182159536615	21.36657139720019
14-15	22.018379303068084	26.033994878834932	26.982635675277432	24.964990142819556
16-17	21.391287437540644	29.12082657327077	27.621503804272468	21.86638218491612
18-19	21.40481479110917	28.190365412380615	29.532159009450304	20.87266078705991
20-21	22.264755442532813	27.306490739701378	29.44879545082279	20.979958366943023
22-23	22.01091374568637	27.170981358955125	29.286124683012876	21.531980212345626
24-25	21.72171474267643	27.377780850434423	29.360601338317142	21.539903068572002
26-27	21.704120489732087	27.51930676217917	29.27481036619513	21.501762381893613
28-29	21.607015138083263	27.52592155314446	29.315496830637827	21.551566478134447
30-31	21.884045015034097	27.42733073994948	29.186927589560874	21.501696655455554
32-33	21.709903793048845	27.56246650093615	29.117177096918024	21.610452609096985
34-35	21.78015292191527	27.59038523268011	29.145149570956384	21.484312274448232
36-37	21.8313598177549	27.488693322699064	29.052634403147998	21.627312456398034
38-39	21.688326028303617	27.675687237886077	29.08773653304796	21.54825020076235
40-41	21.904085548382955	27.55275846451715	28.920403856863462	21.62275213023643
42-43	21.84671428619269	27.640029234672696	28.664972089210224	21.84828438992439
44-45	21.891451504097954	27.772791872099887	28.47071281958689	21.86504380421526
46-47	22.03377766437045	27.72623992868768	28.159273133626385	22.080709273315488
48-49	21.985063004149037	27.84466360678268	28.123862009102794	22.046411379965484
50-51	22.01708716579393	27.977298655606564	27.950493090171573	22.055121088427928
52-53	22.17986984056621	27.90612562856815	27.813694499178986	22.10031003168665
54-55	22.14619764149412	27.80121692185636	27.848990297693238	22.203595138956285
56-57	22.13405440718581	27.96321902501568	27.76959993576762	22.133126632030894
58-59	22.083143041967904	27.974928557251026	27.86573500249534	22.076193398285724
60-61	22.108669350796557	27.859231449042767	27.86042611296399	22.171673087196687
62-63	22.281242541137424	27.926243963037866	27.842266230708674	21.950247265116037
64-65	22.06826113721371	27.854719194976983	27.796585010951176	22.280434656858134
66-67	22.102228044632742	27.89339825816825	27.66449493401857	22.339878763180433
68-69	22.058237098262737	28.05415699699535	27.740819419007188	22.146786485734722
70-71	22.065513722611477	27.839568665180934	27.801698421183758	22.293219191023827
72-73	22.131478262788164	27.889955664771744	27.471256003082424	22.50731006935767
74-75	22.02134438929354	27.98645671891974	27.489161398596863	22.503037493189858
76-77	22.166499485659248	27.696673588532008	27.656687082458077	22.48013984335067
78-79	22.11463658438013	27.674856978089306	27.60984540131888	22.60066103621169
80-81	22.327298851007217	27.69972468646447	27.41960129500954	22.553375167518773
82-83	22.414215278224145	27.850581209805814	27.46145750304819	22.27374600892185
84-85	22.212928177254312	27.6211650848711	27.454736059166905	22.711170678707678
86-87	22.154880201522726	27.784692259405748	27.554334293333277	22.506093245738253
88-89	22.438410378093177	27.534938591426627	27.476048909394528	22.55060212108567
90-91	22.442699948527178	27.748694597469452	27.52818130013833	22.28042415386504
92-93	22.30068495743309	27.674414433144616	27.536020433471165	22.488880175951124
94-95	22.360712488107055	27.462304396614307	27.59598305632491	22.58100005895373
96-97	22.401807721212073	27.687881208977434	27.62196406091971	22.288347008890785
98-99	22.658444535470696	28.08665193134879	27.73138375144912	21.523519781731395
100-101	23.025939244578655	30.8464538334986	24.98830526170415	21.139301660218596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2686.0
1	2266.0
2	2781.0
3	3883.0
4	5205.5
5	7926.5
6	10171.5
7	12361.0
8	14243.0
9	14567.5
10	13450.0
11	12462.0
12	13949.0
13	18844.5
14	30459.0
15	46145.5
16	58464.0
17	64736.0
18	65069.5
19	60896.5
20	54541.0
21	48226.5
22	44678.0
23	46283.0
24	53588.0
25	66157.5
26	82247.0
27	100667.0
28	121769.5
29	144724.5
30	166053.0
31	186011.0
32	208523.5
33	232451.0
34	254424.0
35	274442.5
36	297013.5
37	315377.5
38	328032.0
39	340249.0
40	351437.5
41	361697.0
42	365653.5
43	369628.5
44	375307.0
45	379549.5
46	381542.5
47	379595.0
48	376481.0
49	373019.5
50	367197.5
51	354760.5
52	338516.5
53	320021.5
54	299300.5
55	278377.5
56	255460.0
57	231144.5
58	207124.5
59	178786.5
60	148208.0
61	120806.5
62	96076.0
63	73969.5
64	54737.5
65	40303.0
66	30248.0
67	23137.5
68	17797.5
69	13130.5
70	9453.5
71	6731.5
72	4686.5
73	3278.0
74	2215.5
75	1377.0
76	883.5
77	560.5
78	360.5
79	234.5
80	159.5
81	112.0
82	79.0
83	51.5
84	35.5
85	26.5
86	22.0
87	20.5
88	13.0
89	7.5
90	6.0
91	5.5
92	5.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1299578896675422E-5
2	0.005197097250788803
3	2.1299578896675422E-5
4	2.1299578896675422E-5
5	2.1299578896675422E-5
6	2.1299578896675422E-5
7	2.1299578896675422E-5
8	0.0
9	2.3429536786342966E-4
10-11	8.945823136603677E-4
12-13	0.0
14-15	1.597468417250657E-5
16-17	0.0016081182066989945
18-19	0.0
20-21	0.0
22-23	7.66850133786059E-4
24-25	0.002673707927980748
26-27	0.007074920477734004
28-29	0.3618887737252335
30-31	0.33281846356323136
32-33	0.3317354706744593
34-35	0.18552526498802355
36-37	0.04662888106409702
38-39	0.04482973150250977
40-41	0.025034335020980288
42-43	0.02443494330813237
44-45	0.013285162666267353
46-47	0.009053114195747464
48-49	0.008837544238576626
50-51	0.007517304475850958
52-53	0.006076318444802476
54-55	5.845042709321975E-4
56-57	0.0034484038266429633
58-59	0.002512636147219059
60-61	0.004807995394732874
62-63	0.0011274854342602616
64-65	7.223128022540494E-5
66-67	9.300478774058302E-4
68-69	0.0019345621754157806
70-71	0.004268985023370522
72-73	0.002507195808670623
74-75	0.0018172705925410225
76-77	8.062504631141053E-4
78-79	1.876139107563325E-4
80-81	0.001151734548221326
82-83	0.0013040567949170116
84-85	6.695997367133835E-6
86-87	0.0038562072500627057
88-89	0.0017023420588660274
90-91	0.004676597942018536
92-93	0.013338337269284364
94-95	0.001448720320956098
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	126.0
20-21	452.0
22-23	1058.0
24-25	2242.0
26-27	3605.0
28-29	6578.0
30-31	18044.0
32-33	25043.0
34-35	36165.0
36-37	49405.0
38-39	56782.0
40-41	62800.0
42-43	76371.0
44-45	91610.0
46-47	90654.0
48-49	78242.0
50-51	68859.0
52-53	64794.0
54-55	69709.0
56-57	72218.0
58-59	41902.0
60-61	73634.0
62-63	73620.0
64-65	79443.0
66-67	84352.0
68-69	83338.0
70-71	81774.0
72-73	80431.0
74-75	81948.0
76-77	84022.0
78-79	87975.0
80-81	87892.0
82-83	85810.0
84-85	88352.0
86-87	92409.0
88-89	98037.0
90-91	103484.0
92-93	105929.0
94-95	116471.0
96-97	174001.0
98-99	810356.0
100-101	5899920.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.22503045578031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6920670540856	76.44009146326336
2	7.277523084435032	12.404542530272398
3	1.7132441849034181	4.38033863509747
4	0.6012032308056562	2.049502546221023
5	0.2865646704351924	1.221124138269496
6	0.14819668839317998	0.757804036905273
7	0.08394506848901226	0.5007954713012044
8	0.050724303811163364	0.3458384269723721
9	0.03521171304544794	0.27008273850286485
>10	0.1088847529529063	1.4242022725624732
>50	0.0018447227029241894	0.1028758588581646
>100	5.505554693206236E-4	0.08173310476857049
>500	3.9970471150541424E-5	0.021068777005313965
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0649789448337711E-5	0.0
2	0.0	0.0	0.0	1.0649789448337711E-5	0.0
3	0.0	0.0	0.0	1.0649789448337711E-5	0.0
4	0.0	3.194936834501314E-5	0.0	1.0649789448337711E-5	0.0
5	0.0	3.194936834501314E-5	0.0	1.0649789448337711E-5	0.0
6	0.0	3.194936834501314E-5	0.0	1.0649789448337711E-5	0.0
7	0.0	3.194936834501314E-5	0.0	1.0649789448337711E-5	0.0
8	0.0	6.389873669002627E-5	0.0	2.1299578896675422E-5	0.0
9	0.0	1.1714768393171483E-4	0.0	2.1299578896675422E-5	0.0
10-11	0.0	1.1714768393171483E-4	0.0	2.662447362084428E-5	0.0
12-13	0.0	1.224725786558837E-4	0.0	3.194936834501314E-5	0.0
14-15	0.0	1.4377215755255913E-4	0.0	3.7274263069181994E-5	5.3248947241688556E-6
16-17	0.0	1.4909705227672798E-4	0.0	4.79240525175197E-5	1.0649789448337711E-5
18-19	0.0	1.5442194700089683E-4	0.0	6.922363141419514E-5	1.0649789448337711E-5
20-21	0.0	1.5974684172506568E-4	0.0	1.0117299975920827E-4	1.0649789448337711E-5
22-23	0.0	1.7572152589757223E-4	0.0	4.0469199903683307E-4	2.662447362084428E-5
24-25	0.0	1.916962100700788E-4	0.0	0.0014270717860772534	4.2599157793350844E-5
26-27	0.0	1.916962100700788E-4	0.0	0.0043823883579909685	4.2599157793350844E-5
28-29	0.0	1.916962100700788E-4	0.0	0.019936405847288195	4.2599157793350844E-5
30-31	0.0	2.1299578896675424E-4	0.0	0.02746580698726296	4.2599157793350844E-5
32-33	0.0	2.1299578896675424E-4	0.0	0.030037731139036516	4.2599157793350844E-5
34-35	0.0	2.555949467601051E-4	0.0	0.033658659551471334	4.2599157793350844E-5
36-37	0.0	3.0884389400179365E-4	0.0	0.03863743611856922	4.2599157793350844E-5
38-39	0.0	3.1949368345013135E-4	0.0	0.04787080357027801	4.79240525175197E-5
40-41	0.0	3.3014347289846905E-4	0.0	0.0676368127863928	5.324894724168856E-5
42-43	0.0	3.4611815707097566E-4	0.0	0.07693940386951581	5.324894724168856E-5
44-45	0.0	3.9936710431266417E-4	0.0	0.0894529064713126	5.324894724168856E-5
46-47	0.0	4.41966262106015E-4	0.0	0.10406974248915613	5.324894724168856E-5
48-49	0.0	4.472911568301839E-4	0.0	0.1170837851950248	5.324894724168856E-5
50-51	0.0	5.111898935202102E-4	0.0	0.12966118653351164	5.324894724168856E-5
52-53	0.0	5.111898935202102E-4	0.0	0.14679669775588702	6.389873669002627E-5
54-55	0.0	5.165147882443791E-4	0.0	0.18433720556127745	7.454852613836399E-5
56-57	0.0	5.218396829685479E-4	0.0	0.2252749962006876	7.987342086253284E-5
58-59	0.0	5.218396829685479E-4	0.0	0.25725631391404574	8.519831558670169E-5
60-61	0.0	5.324894724168856E-4	0.0	0.599513922309999	8.519831558670169E-5
62-63	0.0	5.644388407618987E-4	0.0	0.6070433234499737	8.519831558670169E-5
64-65	0.0	7.02886103590289E-4	0.0	0.6147484461158461	8.519831558670169E-5
66-67	0.0	7.02886103590289E-4	0.0	0.6216867839414382	8.519831558670169E-5
68-69	0.0	7.348354719353021E-4	0.0	0.6285026491883743	8.519831558670169E-5
70-71	0.0	7.454852613836398E-4	0.0	0.635116168435792	8.519831558670169E-5
72-73	0.0	7.454852613836398E-4	0.0	0.6393228352678852	1.1714768393171483E-4
74-75	0.0	7.614599455561463E-4	0.0	0.6434283291002195	1.1714768393171483E-4
76-77	0.0	7.934093139011595E-4	0.0	0.6473314769330353	1.1714768393171483E-4
78-79	0.0	8.040591033494971E-4	0.0	0.6508672070298834	1.1714768393171483E-4
80-81	0.0	8.09383998073666E-4	0.0	0.6523528526579265	1.224725786558837E-4
82-83	0.0	8.200337875220037E-4	0.0	0.6530131396037235	1.4377215755255913E-4
84-85	0.0	8.360084716945103E-4	0.0	0.653316658603001	1.4909705227672798E-4
86-87	0.0	8.519831558670169E-4	0.0	0.653439131181657	1.4909705227672798E-4
88-89	0.0	8.786076294878611E-4	0.0	0.6534604307605536	1.4909705227672798E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12415	0.0	53.032745	1
GTATCAA	23715	0.0	38.814236	1
TCAACGC	29930	0.0	30.11065	4
ATCAACG	30105	0.0	30.006624	3
TATCAAC	30710	0.0	29.541714	2
CAACGCA	30770	0.0	29.288963	5
AACGCAG	31525	0.0	28.628199	6
ACGCAGA	36160	0.0	24.923159	7
CGCAGAG	37215	0.0	24.216488	8
GAGACCG	7220	0.0	23.85636	88-89
CGAGACC	7195	0.0	23.659025	88-89
GACCGAG	7575	0.0	23.144701	90-91
AGACCGA	7420	0.0	23.035501	90-91
ACCGAGG	7760	0.0	22.800417	92-93
ATCTCCG	7505	0.0	22.07912	74-75
ACGAGAC	8135	0.0	21.372696	86-87
GCAGAGT	42285	0.0	21.232029	9
GCCCACG	8025	0.0	21.116714	82-83
CCCACGA	7970	0.0	20.998768	84-85
GTGGTAT	6075	0.0	20.972013	1
>>END_MODULE