FastQCFastQC Report
Fri 27 May 2016
SRR2139632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8859656
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.052.8944781
GTATCAA220600.038.886591
TCAACGC273600.030.7092724
ATCAACG276600.030.468943
CAACGCA280300.029.960095
TATCAAC286200.029.5985242
AACGCAG287850.029.2189926
ACGCAGA331850.025.3060537
CGCAGAG341150.024.6161928
GCAGAGT387200.021.5555789
GTGGTAT56050.021.131291
GTACATG273000.020.5292511
GAGTACT232150.020.14862812-13
TGGTATC54200.020.1106912
TACATGG275500.019.8600882
AGACCGA84250.019.54159490-91
ACATGGG274600.019.4243
GAGACCG84400.019.3948588-89
CGAGACC83700.019.31855288-89
AGAGTAC355050.019.24742710-11
Produced by FastQC (version 0.11.5)