##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8859656
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92519325806781	34.0	31.0	34.0	31.0	34.0
2	33.05525485413881	34.0	33.0	34.0	31.0	34.0
3	33.113427993141045	34.0	33.0	34.0	31.0	34.0
4	36.4735332839108	37.0	37.0	37.0	35.0	37.0
5	36.43974416162433	37.0	37.0	37.0	35.0	37.0
6	36.46731295210559	37.0	37.0	37.0	35.0	37.0
7	36.44195948465719	37.0	37.0	37.0	35.0	37.0
8	36.42864485934894	37.0	37.0	37.0	35.0	37.0
9	38.22254577378625	39.0	39.0	39.0	37.0	39.0
10-11	38.16254462927229	39.0	39.0	39.0	37.0	39.0
12-13	38.13554437102299	39.0	39.0	39.0	37.0	39.0
14-15	39.64271654565369	41.0	40.0	41.0	37.0	41.0
16-17	39.61586296352816	41.0	40.0	41.0	37.0	41.0
18-19	39.62669114918232	41.0	40.0	41.0	37.0	41.0
20-21	39.6209463334433	41.0	40.0	41.0	37.0	41.0
22-23	39.56585817131867	41.0	40.0	41.0	37.0	41.0
24-25	39.5019524752895	41.0	39.5	41.0	37.0	41.0
26-27	39.45073602728908	41.0	39.0	41.0	36.5	41.0
28-29	39.277335882990656	41.0	39.0	41.0	36.0	41.0
30-31	39.19339186402975	41.0	39.0	41.0	36.0	41.0
32-33	39.08723033519284	41.0	39.0	41.0	35.0	41.0
34-35	38.99096659959045	40.0	39.0	41.0	35.0	41.0
36-37	39.08765917312448	40.5	39.0	41.0	35.0	41.0
38-39	39.109763247440206	41.0	39.0	41.0	35.0	41.0
40-41	39.10756266272614	41.0	39.0	41.0	35.0	41.0
42-43	39.06566769238437	40.5	39.0	41.0	35.0	41.0
44-45	38.99886658774851	40.0	39.0	41.0	35.0	41.0
46-47	38.91154796269407	40.0	38.5	41.0	35.0	41.0
48-49	38.83585894711078	40.0	38.0	41.0	35.0	41.0
50-51	38.745609343935605	40.0	38.0	41.0	35.0	41.0
52-53	38.64140926016486	40.0	38.0	41.0	35.0	41.0
54-55	38.5219800750036	40.0	38.0	41.0	34.5	41.0
56-57	38.35157458536047	40.0	37.5	41.0	34.0	41.0
58-59	38.19314505493625	40.0	37.0	41.0	34.0	41.0
60-61	38.00513608089986	40.0	36.5	41.0	34.0	41.0
62-63	37.75830191597193	39.0	36.0	41.0	34.0	41.0
64-65	37.46444743594448	39.0	35.5	40.5	33.0	41.0
66-67	37.14556848203591	38.0	35.0	40.0	33.0	41.0
68-69	36.83963926276183	37.5	35.0	40.0	33.0	41.0
70-71	36.5147611935424	37.0	35.0	39.5	32.5	41.0
72-73	36.17300442989334	36.5	35.0	39.0	32.0	40.5
74-75	35.80641051186082	36.0	35.0	39.0	32.0	40.0
76-77	34.86656742801367	35.0	34.0	37.0	30.5	39.0
78-79	35.097924837692545	35.0	34.0	37.0	31.0	39.0
80-81	34.91303706596557	35.0	34.0	37.0	31.0	39.0
82-83	34.63817984111023	35.0	34.0	36.0	31.0	38.5
84-85	34.385409310938954	35.0	34.0	36.0	31.0	37.0
86-87	34.1355129691391	35.0	34.0	35.5	31.0	37.0
88-89	33.83511413326623	35.0	34.0	35.0	31.0	36.5
90-91	33.75185305568738	35.0	34.0	35.0	31.0	36.0
92-93	33.66512355687588	35.0	34.0	35.0	31.0	36.0
94-95	33.55355422724037	35.0	33.5	35.0	30.5	35.5
96-97	33.51467981220481	35.0	33.0	35.0	30.0	35.0
98-99	33.52129268529768	34.5	33.0	35.0	30.5	35.0
100-101	32.47901936730027	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	2.0
20	26.0
21	128.0
22	698.0
23	2364.0
24	6423.0
25	13473.0
26	23853.0
27	38399.0
28	55074.0
29	75268.0
30	97264.0
31	123302.0
32	157519.0
33	207307.0
34	303297.0
35	460168.0
36	863372.0
37	2050380.0
38	2961499.0
39	1353315.0
40	66520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.41455275792937	21.046950591975712	15.374979654961695	24.163516995133218
2	13.776509339624655	21.467393911564592	41.44260834710717	23.313488401703587
3	16.84201211469432	26.568012701173206	31.307136824982106	25.282838359150368
4	11.95280312301135	18.747605719139823	39.092305410572465	30.207285747276362
5	12.987775820590736	37.916345265853494	35.76368783701937	13.332191076536398
6	29.478431099002282	38.38408362222723	18.788318369995036	13.349166908775445
7	26.04117497139279	33.041414484132225	23.197474754657463	17.719935789817526
8	24.460026942358365	36.41579722912461	21.2347546264499	17.889421202067123
9	25.408707536065815	16.900005824167042	21.44365750466498	36.247629135102166
10-11	23.868230193361732	27.03758239732746	29.033262444020387	20.060924965290422
12-13	24.839677712019	24.616830408952765	29.046489851635286	21.497002027392945
14-15	22.036222974182905	25.950693218419886	26.859351779389566	25.153732028007642
16-17	21.460890549749973	29.12750143842321	27.472898526473337	21.93870948535348
18-19	21.488936165625873	28.16042657193912	29.373735736534645	20.97690152590036
20-21	22.32499613266868	27.247661640198846	29.311871248258292	21.115470978874185
22-23	22.0460342986048	27.195227427324692	29.149078666083593	21.60965960798692
24-25	21.846099098168942	27.331509352918985	29.235528588428945	21.586862960483128
26-27	21.742009543808184	27.53214797007874	29.163608713012266	21.562233773100807
28-29	21.65613090360796	27.480225282758568	29.21114694182051	21.65249687181296
30-31	21.984781520148243	27.392038284821652	29.10021682492916	21.522963370100946
32-33	21.842669429301544	27.500964132892076	29.044918360228245	21.611448077578135
34-35	21.80458348354518	27.523551879542254	29.132712812542394	21.539151824370176
36-37	21.8670099281312	27.520595202684717	28.976117196596817	21.636277672587266
38-39	21.72263745341061	27.621010163716175	29.022247542581457	21.634104840291755
40-41	21.933943814086206	27.499259140714077	28.874887471080797	21.691909574118924
42-43	21.899415146752755	27.59944981522934	28.641625842268432	21.85950919574947
44-45	21.91602890776381	27.7352272997057	28.465278178753433	21.88346561377706
46-47	22.10286949159857	27.65164370927756	28.109992293961138	22.135494505162725
48-49	21.996756477274722	27.777608458353814	28.102791392055458	22.122843672316
50-51	22.003075012926033	27.916356081363176	27.986552226263207	22.094016679447588
52-53	22.219274786825554	27.83192155899125	27.85325235804274	22.09555129614045
54-55	22.1686000899898	27.760666998182288	27.84486047148223	22.225872440345682
56-57	22.160206597322258	27.892290100257384	27.775187060120228	22.17231624230013
58-59	22.071530207097098	27.956474192085203	27.893618078899458	22.07837752191824
60-61	22.122379011649006	27.89631986500092	27.82721925755125	22.154081865798826
62-63	22.241401241797963	28.006095641112832	27.777981385624866	21.97452173146434
64-65	22.073060771621943	27.912112512287308	27.76218735099436	22.252639365096385
66-67	22.140363502487638	27.92999078319733	27.63012079335056	22.299524920964476
68-69	22.068815207132836	28.042210188343102	27.754768021291166	22.134206583232892
70-71	22.09394145606057	27.820591959734607	27.798768058787243	22.28669852541758
72-73	22.14707435337993	27.862201584361877	27.516679510272773	22.47404455198542
74-75	22.037580380302337	27.957957104624164	27.490206813893643	22.514255701179852
76-77	22.16523112518732	27.663564283810633	27.6738380413866	22.497366549615442
78-79	22.158881288491138	27.649845715525736	27.60353178439871	22.58774121158442
80-81	22.33013677081815	27.706849330527483	27.396517017115997	22.56649688153837
82-83	22.43195327689777	27.832057963093803	27.453314881997066	22.282673878011362
84-85	22.252480788670418	27.59153650055916	27.45631889804898	22.69966381272144
86-87	22.13923204256741	27.796098883747977	27.547374960615574	22.517294113069042
88-89	22.43900036464121	27.570158869508564	27.48854663691932	22.50229412893091
90-91	22.439624454763916	27.775121638145468	27.51885320129831	22.266400705792307
92-93	22.292860245969155	27.687622326043943	27.548755289622434	22.47076213836447
94-95	22.390757005634867	27.476312300681847	27.57946468716186	22.553466006521422
96-97	22.393740070325237	27.69615966535182	27.644290908579944	22.265809355743
98-99	22.64753510134338	28.098124613092857	27.78188370866022	21.472456576903546
100-101	23.039566997003025	30.90988059318437	24.98276790144031	21.0677845083723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2329.0
1	1988.5
2	2456.5
3	3513.0
4	4748.5
5	7184.0
6	9223.5
7	11111.5
8	12795.5
9	13199.5
10	12208.5
11	11162.0
12	12405.5
13	16672.5
14	27002.0
15	41141.0
16	52420.0
17	58384.0
18	58728.5
19	54929.0
20	49141.5
21	43385.5
22	40225.0
23	41712.5
24	48320.0
25	59893.5
26	75223.5
27	92361.5
28	111546.5
29	132358.5
30	151571.5
31	171115.5
32	192964.0
33	214919.5
34	236297.0
35	256247.5
36	277856.5
37	295632.5
38	308055.0
39	319941.0
40	331647.0
41	341974.5
42	346035.0
43	349735.5
44	354272.0
45	356451.5
46	356930.5
47	355903.5
48	355594.5
49	353869.5
50	348523.5
51	337586.0
52	321295.5
53	303642.5
54	284557.0
55	264023.0
56	241869.5
57	218266.0
58	194786.5
59	169408.0
60	142323.0
61	116129.0
62	92205.5
63	71173.0
64	52889.5
65	39004.5
66	29487.5
67	22723.5
68	17454.0
69	12896.5
70	9330.0
71	6805.0
72	4862.0
73	3363.0
74	2289.0
75	1465.0
76	939.5
77	605.0
78	368.5
79	228.0
80	152.0
81	106.5
82	75.5
83	49.5
84	35.0
85	25.5
86	18.5
87	15.0
88	11.5
89	6.5
90	4.5
91	4.0
92	3.5
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2574239902768235E-5
2	0.005271085017296382
3	2.2574239902768235E-5
4	2.2574239902768235E-5
5	2.2574239902768235E-5
6	2.2574239902768235E-5
7	2.2574239902768235E-5
8	1.1287119951384118E-5
9	2.2574239902768234E-4
10-11	7.844548366211961E-4
12-13	2.2574239902768235E-5
14-15	2.8217799878460292E-5
16-17	0.001450394913752859
18-19	1.6930679927076178E-5
20-21	0.0
22-23	8.466099648702017E-4
24-25	0.0025006802075959716
26-27	0.007413640963861408
28-29	0.3650477588052535
30-31	0.3355001321136022
32-33	0.33444823633088017
34-35	0.1863162263245265
36-37	0.04719500385655982
38-39	0.045465281362969394
40-41	0.025419169551575027
42-43	0.025059190132537084
44-45	0.01325803877032687
46-47	0.008843668971880861
48-49	0.0088317178898337
50-51	0.007543256994006063
52-53	0.006148768015723365
54-55	5.930804992441587E-4
56-57	0.003346217400108632
58-59	0.0025474841729779033
60-61	0.0049640571045148196
62-63	0.0012216321584968231
64-65	5.0819909360150666E-5
66-67	7.183538511014096E-4
68-69	0.0015289932428216844
70-71	0.0039130980749978615
72-73	0.002054670487340752
74-75	0.0013273971775800615
76-77	5.052642130991567E-4
78-79	2.110124684544868E-4
80-81	0.0011835412351959291
82-83	0.0011061218137865493
84-85	0.0
86-87	0.003663533669581701
88-89	0.0018433338997744797
90-91	0.004989435381195052
92-93	0.013305734719890985
94-95	0.0013963665191848373
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	105.0
20-21	474.0
22-23	962.0
24-25	2182.0
26-27	3275.0
28-29	6028.0
30-31	16599.0
32-33	22757.0
34-35	32914.0
36-37	45563.0
38-39	52438.0
40-41	56659.0
42-43	69001.0
44-45	82885.0
46-47	81764.0
48-49	71324.0
50-51	62862.0
52-53	58779.0
54-55	62840.0
56-57	66391.0
58-59	39202.0
60-61	67862.0
62-63	68060.0
64-65	73149.0
66-67	78591.0
68-69	77271.0
70-71	73798.0
72-73	71791.0
74-75	73652.0
76-77	75409.0
78-79	78808.0
80-81	79625.0
82-83	77784.0
84-85	79640.0
86-87	83520.0
88-89	88008.0
90-91	94168.0
92-93	96531.0
94-95	107854.0
96-97	161322.0
98-99	769228.0
100-101	5648581.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.69956745809992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.09530969742777	77.21129071073116
2	7.061630024542977	12.103572773049294
3	1.5994166605002007	4.112079479704376
4	0.5692283785129729	1.9513050329374948
5	0.257498010993302	1.103373408172352
6	0.14057643196574043	0.722840364856018
7	0.08245021889890515	0.4946163667523279
8	0.04919599842697737	0.33728606286890594
9	0.030151727507779634	0.2325591004978087
>10	0.11151031188082093	1.461176189691312
>50	0.0024935747170551762	0.13979630993535588
>100	4.3399166195930356E-4	0.059664793053518954
>500	8.397873836340895E-5	0.047067851447460556
>1k	2.0994225217006925E-5	0.023371556302598403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.2574239902768235E-5	0.0	1.1287119951384118E-5	0.0
5	0.0	3.386135985415235E-5	0.0	1.1287119951384118E-5	0.0
6	0.0	4.514847980553647E-5	0.0	2.2574239902768235E-5	1.1287119951384118E-5
7	0.0	4.514847980553647E-5	0.0	2.2574239902768235E-5	1.1287119951384118E-5
8	0.0	4.514847980553647E-5	0.0	2.2574239902768235E-5	1.1287119951384118E-5
9	0.0	1.354454394166094E-4	0.0	3.386135985415235E-5	1.1287119951384118E-5
10-11	0.0	1.354454394166094E-4	0.0	3.386135985415235E-5	1.1287119951384118E-5
12-13	0.0	1.354454394166094E-4	0.0	3.386135985415235E-5	1.1287119951384118E-5
14-15	0.0	1.6930679927076175E-4	0.0	3.386135985415235E-5	2.2574239902768235E-5
16-17	0.0	1.9188103917352998E-4	0.0	5.6435599756920584E-5	2.2574239902768235E-5
18-19	0.0	2.0881171910060615E-4	0.0	1.0158407956245705E-4	3.9504919829844406E-5
20-21	0.0	2.4267307895475853E-4	0.0	2.0881171910060615E-4	4.514847980553647E-5
22-23	0.0	2.991086787116791E-4	0.0	6.095044773747424E-4	5.079203978122853E-5
24-25	0.0	3.103957986630632E-4	0.0	0.0016761373127805413	6.77227197083047E-5
26-27	0.0	3.1603935863875525E-4	0.0	0.004729303259629945	6.77227197083047E-5
28-29	0.0	3.216829186144473E-4	0.0	0.019978202313949887	7.336627968399675E-5
30-31	0.0	4.063363182498282E-4	0.0	0.0276421567609397	7.900983965968881E-5
32-33	0.0	4.063363182498282E-4	0.0	0.03020997654987959	1.0158407956245705E-4
34-35	0.0	4.5712835803105674E-4	0.0	0.03350017201570806	1.0158407956245705E-4
36-37	0.0	5.361381976907455E-4	0.0	0.038308485114997695	1.0158407956245705E-4
38-39	0.0	5.7564311752059E-4	0.0	0.04656501335943518	1.0158407956245705E-4
40-41	0.0	6.546529571802788E-4	0.0	0.06597321611584017	1.0158407956245705E-4
42-43	0.0	6.77227197083047E-4	0.0	0.07458528863874625	1.0158407956245705E-4
44-45	0.0	7.393063568156596E-4	0.0	0.08660607138697032	1.0158407956245705E-4
46-47	0.0	7.78811276645504E-4	0.0	0.10016754600855834	1.0158407956245705E-4
48-49	0.0	7.900983965968882E-4	0.0	0.11254951659522672	1.1287119951384117E-4
50-51	0.0	8.860389161836532E-4	0.0	0.12376327026692685	1.1287119951384117E-4
52-53	0.0	8.916824761593452E-4	0.0	0.1394918719191806	1.1851475948953323E-4
54-55	0.0	9.142567160621135E-4	0.0	0.17404174609036738	1.241583194652253E-4
56-57	0.0	9.368309559648817E-4	0.0	0.21215270660621588	1.2980187944091736E-4
58-59	0.0	9.481180759162659E-4	0.0	0.24232882179624132	1.4108899939230146E-4
60-61	0.0	9.763358757947261E-4	0.0	0.5702027257040228	1.4673255936799352E-4
62-63	0.0	9.819794357704182E-4	0.0	0.5769129185151207	1.4673255936799352E-4
64-65	0.0	0.001072276395381491	0.0	0.5834086560471423	1.5237611934368556E-4
66-67	0.0	0.0010892070753085671	0.0	0.5893908296213758	1.5801967931937763E-4
68-69	0.0	0.0011174248751870276	0.0	0.5957059732341752	1.636632392950697E-4
70-71	0.0	0.001145642675065488	0.0	0.6013156718500131	1.6930679927076175E-4
72-73	0.0	0.001162573354992564	0.0	0.6055257675918795	2.031681591249141E-4
74-75	0.0	0.0011907911548710245	0.0	0.609143289536298	2.031681591249141E-4
76-77	0.0	0.0012133653947737928	0.0	0.6124165543221994	2.031681591249141E-4
78-79	0.0	0.0012246525147251767	0.0	0.6154358589091946	2.031681591249141E-4
80-81	0.0	0.0012302960747008688	0.0	0.6166774421038469	2.2009883905199027E-4
82-83	0.0	0.0012302960747008688	0.0	0.6169539765426558	2.3702951897906646E-4
84-85	0.0	0.0012302960747008688	0.0	0.6172361545414404	2.3702951897906646E-4
86-87	0.0	0.001264157434555021	0.0	0.6173320950610273	2.3702951897906646E-4
88-89	0.0	0.0012867316744577893	0.0	0.6173828871008085	2.3702951897906646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11845	0.0	52.894478	1
GTATCAA	22060	0.0	38.88659	1
TCAACGC	27360	0.0	30.709272	4
ATCAACG	27660	0.0	30.46894	3
CAACGCA	28030	0.0	29.96009	5
TATCAAC	28620	0.0	29.598524	2
AACGCAG	28785	0.0	29.218992	6
ACGCAGA	33185	0.0	25.306053	7
CGCAGAG	34115	0.0	24.616192	8
GCAGAGT	38720	0.0	21.555578	9
GTGGTAT	5605	0.0	21.13129	1
GTACATG	27300	0.0	20.529251	1
GAGTACT	23215	0.0	20.148628	12-13
TGGTATC	5420	0.0	20.110691	2
TACATGG	27550	0.0	19.860088	2
AGACCGA	8425	0.0	19.541594	90-91
ACATGGG	27460	0.0	19.424	3
GAGACCG	8440	0.0	19.39485	88-89
CGAGACC	8370	0.0	19.318552	88-89
AGAGTAC	35505	0.0	19.247427	10-11
>>END_MODULE