FastQCFastQC Report
Fri 27 May 2016
SRR2139631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8169973
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111500.053.9041251
GTATCAA210950.039.2623631
TCAACGC271750.030.050224
ATCAACG273450.029.894393
TATCAAC280500.029.4049912
CAACGCA279300.029.1920435
AACGCAG286900.028.508396
ACGCAGA327550.024.9442377
CGAGACC57750.024.62796688-89
CGCAGAG334600.024.393048
GAGACCG58600.024.31956788-89
AGACCGA60400.023.60138790-91
GACCGAG61650.023.40598990-91
ACCGAGG64100.022.90972792-93
TGGTATC52500.022.8637542
ACGAGAC63250.022.84811886-87
ATCTCCG60750.022.58099774-75
GTGGTAT55700.022.4737991
CCCACGA63250.022.0433884-85
GCCCACG64400.021.66632582-83
Produced by FastQC (version 0.11.5)