##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8169973
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92237526366366	34.0	31.0	34.0	31.0	34.0
2	33.053565538099086	34.0	33.0	34.0	31.0	34.0
3	33.11210171686002	34.0	33.0	34.0	31.0	34.0
4	36.471986137530685	37.0	37.0	37.0	35.0	37.0
5	36.437077699032784	37.0	37.0	37.0	35.0	37.0
6	36.466807540245235	37.0	37.0	37.0	35.0	37.0
7	36.442280164206174	37.0	37.0	37.0	35.0	37.0
8	36.42982871546822	37.0	37.0	37.0	35.0	37.0
9	38.223308326722744	39.0	39.0	39.0	37.0	39.0
10-11	38.163787077386914	39.0	39.0	39.0	37.0	39.0
12-13	38.13586464728831	39.0	39.0	39.0	37.0	39.0
14-15	39.64309478134138	41.0	40.0	41.0	37.0	41.0
16-17	39.616566419497346	41.0	40.0	41.0	37.0	41.0
18-19	39.63026126279732	41.0	40.0	41.0	37.0	41.0
20-21	39.62645994110824	41.0	40.0	41.0	37.0	41.0
22-23	39.56898128066702	41.0	40.0	41.0	37.0	41.0
24-25	39.505304457067524	41.0	39.5	41.0	37.0	41.0
26-27	39.45571693169643	41.0	39.0	41.0	36.5	41.0
28-29	39.284080601468254	41.0	39.0	41.0	36.0	41.0
30-31	39.195726778181424	41.0	39.0	41.0	36.0	41.0
32-33	39.08699537493401	41.0	39.0	41.0	35.0	41.0
34-35	38.990425868333574	40.0	39.0	41.0	35.0	41.0
36-37	39.084374009941556	40.5	39.0	41.0	35.0	41.0
38-39	39.10913632975952	41.0	39.0	41.0	35.0	41.0
40-41	39.10209302022426	41.0	39.0	41.0	35.0	41.0
42-43	39.05876335039026	40.5	39.0	41.0	35.0	41.0
44-45	38.99133250294288	40.0	39.0	41.0	35.0	41.0
46-47	38.905737100737184	40.0	38.5	41.0	35.0	41.0
48-49	38.82932642849367	40.0	38.0	41.0	35.0	41.0
50-51	38.7405583790902	40.0	38.0	41.0	35.0	41.0
52-53	38.635871423033564	40.0	38.0	41.0	35.0	41.0
54-55	38.51577662424966	40.0	38.0	41.0	34.5	41.0
56-57	38.3473194787843	40.0	37.5	41.0	34.0	41.0
58-59	38.189651303862505	40.0	37.0	41.0	34.0	41.0
60-61	38.00337306793256	40.0	36.5	41.0	34.0	41.0
62-63	37.75734384331792	39.0	36.0	41.0	34.0	41.0
64-65	37.46505778456205	39.0	35.5	40.5	33.0	41.0
66-67	37.14869961479841	38.0	35.0	40.0	33.0	41.0
68-69	36.84184859503895	37.5	35.0	40.0	33.0	41.0
70-71	36.51979584331842	37.0	35.0	39.5	32.5	41.0
72-73	36.179774331020035	36.5	35.0	39.0	32.0	41.0
74-75	35.8161360314473	36.0	35.0	39.0	32.0	40.0
76-77	34.87434316937633	35.0	34.0	37.0	30.5	39.0
78-79	35.108116619095284	35.0	34.0	37.0	31.0	39.0
80-81	34.923323841795074	35.0	34.0	37.0	31.0	39.0
82-83	34.64763492765212	35.0	34.0	36.0	31.0	38.5
84-85	34.39456911266872	35.0	34.0	36.0	31.0	37.0
86-87	34.14469870363064	35.0	34.0	35.5	31.0	37.0
88-89	33.841437002739355	35.0	34.0	35.0	31.0	36.5
90-91	33.755468165811024	35.0	34.0	35.0	31.0	36.0
92-93	33.67093445180548	35.0	34.0	35.0	31.0	36.0
94-95	33.559406018797276	35.0	33.5	35.0	30.5	35.5
96-97	33.5185313766049	35.0	33.0	35.0	30.0	35.0
98-99	33.524143274046104	35.0	33.0	35.0	30.5	35.0
100-101	32.470716921577214	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	24.0
21	154.0
22	697.0
23	2295.0
24	6083.0
25	12323.0
26	22367.0
27	35420.0
28	50730.0
29	69181.0
30	89893.0
31	114292.0
32	144734.0
33	190897.0
34	277978.0
35	425167.0
36	798986.0
37	1886107.0
38	2724632.0
39	1256254.0
40	61756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50749898439077	21.08746259014565	15.401348327589332	24.00369009787425
2	13.783398337064881	21.559144582473582	41.447740390482636	23.209716689978897
3	16.827754510327022	26.691275479123373	31.36313914378909	25.117830866760514
4	11.910528957684438	18.73120021326876	39.24322638520348	30.115044443843324
5	12.967141996674897	37.906502261390585	35.77102396788827	13.355331774046256
6	29.322520404902196	38.53017629311627	18.844933759267015	13.302369542714523
7	26.006267095374735	33.09815099756144	23.31751891958517	17.57806298747866
8	24.420239822089986	36.406693143294355	21.269898933570527	17.903168101045132
9	25.388395937355302	16.9351485547829	21.506609159983743	36.16984634787806
10-11	23.821019249118702	27.048547006015305	29.10737717744995	20.02305656741604
12-13	24.82482500248165	24.703729131050984	29.118296963772096	21.35314890269527
14-15	22.000639781913563	26.01367703669468	26.98885179709623	24.996831384295525
16-17	21.365222629016188	29.175613692643203	27.609495012574897	21.849668665765712
18-19	21.39829242988838	28.160665666908034	29.56060926524595	20.880432637957632
20-21	22.249097651445716	27.25000832334532	29.534613610725952	20.966280414483013
22-23	21.99004394737968	27.170048382329654	29.361033633458543	21.478874036832117
24-25	21.72941178559122	27.357841506938215	29.40728624123874	21.505460466231828
26-27	21.679921531396747	27.512992780183076	29.322907022200273	21.484178666219897
28-29	21.608754784798865	27.47038622799294	29.381268684400563	21.539590302807635
30-31	21.87955858486945	27.413784819198344	29.255850692112805	21.4508059038194
32-33	21.724695411425813	27.54061751180651	29.149808776276043	21.584878300491642
34-35	21.768832448907233	27.553434694193996	29.179326897911253	21.49840595898752
36-37	21.80030521515366	27.49620028362716	29.111648376595667	21.59184612462352
38-39	21.66650000724922	27.64502029438794	29.13697780040557	21.55150189795727
40-41	21.88569398640207	27.575415780501487	28.932404774508385	21.606485458588054
42-43	21.821901711508975	27.66547236592852	28.70893584269032	21.803690079872183
44-45	21.891806905064954	27.787729893231468	28.50645565639858	21.814007545305003
46-47	22.001738369207875	27.754590178592693	28.21223278639299	22.031438665806448
48-49	21.947419853172875	27.874364490947702	28.140396885653306	22.037818770226117
50-51	21.989301266095023	27.983968558545484	27.982348349944935	22.044381825414554
52-53	22.145223527231302	27.90271719371184	27.854717237940513	22.09734204111634
54-55	22.149581707849713	27.839008636863756	27.84086494336011	22.170544711926425
56-57	22.09896075524141	27.972333759227208	27.794583594797786	22.134121890733596
58-59	22.013992752677595	27.996284246643405	27.927649655307896	22.062073345371108
60-61	22.09401170432931	27.849465051616257	27.903737384999623	22.15278585905481
62-63	22.203940295573364	27.95726602489937	27.86438655660651	21.97440712292076
64-65	22.046240297701246	27.868335549487007	27.83192976933964	22.253494383472106
66-67	22.12409613695514	27.88783248202212	27.66954535665389	22.31852602436885
68-69	22.041207750601664	28.07589712737744	27.77699211115064	22.10590301087025
70-71	22.01968729130288	27.829864703480933	27.87202147719972	22.278426528016464
72-73	22.083879095001897	27.85518169390107	27.585371440023476	22.475567771073557
74-75	22.01484450031434	27.9893218926279	27.52563451851855	22.47019908853921
76-77	22.131988469551256	27.723435656783522	27.695705177190753	22.44887069647446
78-79	22.0887977300616	27.719074099414186	27.640225238200795	22.551902932323422
80-81	22.30570369973159	27.716994932316908	27.426436034345002	22.5508653336065
82-83	22.352510107346678	27.877696241335403	27.50140900341494	22.268384647902977
84-85	22.223324343127256	27.630592114668012	27.49771750421714	22.648366037987593
86-87	22.129791470856777	27.80097030692964	27.586044128896468	22.48319409331711
88-89	22.3942371230027	27.582272464887943	27.52646858410165	22.497021828007707
90-91	22.40613216578399	27.80506947224512	27.52907928902701	22.259719072943877
92-93	22.256457480483284	27.730848066495184	27.599987674665083	22.412706778356455
94-95	22.321834118049562	27.502598523973216	27.636687248497093	22.538880109480132
96-97	22.371706840939947	27.713233398407638	27.6800843709048	22.234975389747618
98-99	22.607053493451474	28.179420829750395	27.760245246015298	21.453280430782833
100-101	22.987428547159357	30.950996044393907	25.009189206928973	21.052386201517763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2410.0
1	2032.5
2	2389.0
3	3255.5
4	4340.0
5	6748.5
6	8845.0
7	10891.0
8	12614.0
9	12826.5
10	11672.0
11	10587.0
12	11818.5
13	15959.5
14	25975.0
15	39783.0
16	50691.5
17	56490.0
18	57060.5
19	53356.5
20	47560.5
21	41868.5
22	38510.5
23	39649.5
24	45769.5
25	56792.5
26	71029.5
27	87130.5
28	105945.5
29	126109.5
30	145130.0
31	163954.0
32	184273.5
33	204514.0
34	222490.5
35	239072.0
36	258356.0
37	274357.5
38	284260.5
39	293498.5
40	303489.0
41	312201.5
42	315690.5
43	319715.5
44	324545.0
45	326914.5
46	327431.0
47	324839.5
48	321424.5
49	318471.0
50	314117.5
51	304099.5
52	288516.0
53	272384.0
54	256537.0
55	238951.5
56	219783.5
57	199501.5
58	178543.5
59	155253.5
60	130445.0
61	107252.0
62	85789.0
63	66258.5
64	49386.5
65	36720.0
66	27828.5
67	21498.5
68	16557.5
69	12315.5
70	8888.0
71	6296.0
72	4521.5
73	3280.5
74	2300.5
75	1497.0
76	958.0
77	569.0
78	343.0
79	224.0
80	149.0
81	107.0
82	76.0
83	45.0
84	24.5
85	15.0
86	10.0
87	7.5
88	7.5
89	7.5
90	5.5
91	4.5
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005410054598711648
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.937586207445239E-4
10-11	8.139561783129517E-4
12-13	0.0
14-15	1.8359913796532744E-5
16-17	0.0015422327589087504
18-19	1.2239942531021829E-5
20-21	1.2240212206110975E-5
22-23	7.22219593805339E-4
24-25	0.0024914147195475053
26-27	0.007145579705818871
28-29	0.3662460226769815
30-31	0.3370708047962476
32-33	0.3354258488504701
34-35	0.1865979011771699
36-37	0.047615736490542675
38-39	0.045901005959729395
40-41	0.026110305874756254
42-43	0.0257102285255647
44-45	0.013871299125637943
46-47	0.009319388737955155
48-49	0.009161519467159807
50-51	0.007786847844143889
52-53	0.006785218227689152
54-55	7.955535979044058E-4
56-57	0.0033676578293935187
58-59	0.0028361153761376125
60-61	0.005510196469642846
62-63	0.0015972255782161367
64-65	4.8192197091215366E-5
66-67	8.408684878905209E-4
68-69	0.001761577812260722
70-71	0.004430270100454425
72-73	0.0022831999641559075
74-75	0.0015321106175193522
76-77	7.518341468470776E-4
78-79	1.9179216574737975E-4
80-81	0.0010963240775738034
82-83	0.001206517668772593
84-85	1.5248403739914992E-5
86-87	0.003655755547493355
88-89	0.0016084251495542593
90-91	0.005118578962229081
92-93	0.013727703814079234
94-95	0.0015232494707115374
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	429.0
22-23	915.0
24-25	2008.0
26-27	2999.0
28-29	5474.0
30-31	14795.0
32-33	20816.0
34-35	30467.0
36-37	40888.0
38-39	47398.0
40-41	52422.0
42-43	63524.0
44-45	77986.0
46-47	75748.0
48-49	65436.0
50-51	57795.0
52-53	54587.0
54-55	58012.0
56-57	60708.0
58-59	35710.0
60-61	61390.0
62-63	61677.0
64-65	65906.0
66-67	70797.0
68-69	70947.0
70-71	68645.0
72-73	66797.0
74-75	68323.0
76-77	70780.0
78-79	73659.0
80-81	74151.0
82-83	72380.0
84-85	74158.0
86-87	77931.0
88-89	82657.0
90-91	87763.0
92-93	90534.0
94-95	100223.0
96-97	149466.0
98-99	709537.0
100-101	5204031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.8919256311553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2300859206718	77.50035829591096
2	6.906153863788492	11.86365708131674
3	1.6125174031220213	4.1550667460370105
4	0.5739670364485268	1.9719653603748586
5	0.2624520235260367	1.1271254843222283
6	0.14013545562363006	0.7221902479627738
7	0.08014721171934992	0.4818798843980004
8	0.04931315558959218	0.3388481514031074
9	0.03509679150654724	0.2713077905375267
>10	0.10831656804575658	1.404351510751351
>50	0.0013929757335816573	0.07612412251918778
>100	3.992146469613528E-4	0.07163219076958408
>500	2.2379577711018164E-5	0.015493133696756149
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2239942531021829E-5	0.0
2	0.0	1.2239942531021829E-5	0.0	1.2239942531021829E-5	0.0
3	0.0	1.2239942531021829E-5	0.0	1.2239942531021829E-5	0.0
4	0.0	6.119971265510914E-5	0.0	2.4479885062043657E-5	0.0
5	0.0	6.119971265510914E-5	0.0	2.4479885062043657E-5	0.0
6	3.671982759306549E-5	6.119971265510914E-5	0.0	2.4479885062043657E-5	0.0
7	3.671982759306549E-5	6.119971265510914E-5	0.0	3.671982759306549E-5	1.2239942531021829E-5
8	3.671982759306549E-5	7.343965518613097E-5	0.0	3.671982759306549E-5	1.2239942531021829E-5
9	4.8959770124087314E-5	1.4687931037226195E-4	0.0	4.8959770124087314E-5	1.2239942531021829E-5
10-11	4.8959770124087314E-5	1.4687931037226195E-4	0.0	6.119971265510914E-5	1.2239942531021829E-5
12-13	4.8959770124087314E-5	1.4687931037226195E-4	0.0	6.119971265510914E-5	1.2239942531021829E-5
14-15	4.8959770124087314E-5	1.5911925290328376E-4	0.0	7.343965518613097E-5	1.2239942531021829E-5
16-17	1.1627945404470737E-4	1.5911925290328376E-4	0.0	1.1015948277919645E-4	2.4479885062043657E-5
18-19	1.2239942531021828E-4	1.713591954343056E-4	0.0	1.8359913796532742E-4	2.4479885062043657E-5
20-21	1.2239942531021828E-4	1.8359913796532742E-4	0.0	2.8151867821350204E-4	2.4479885062043657E-5
22-23	1.2239942531021828E-4	1.9583908049634926E-4	0.0	5.875172414890478E-4	2.4479885062043657E-5
24-25	1.2239942531021828E-4	2.5091882188594747E-4	0.0	0.0016952320405465232	3.059985632755457E-5
26-27	1.2239942531021828E-4	2.6927873568248023E-4	0.0	0.004645058190522784	3.671982759306549E-5
28-29	1.2239942531021828E-4	2.6927873568248023E-4	0.0	0.019914386497972517	3.671982759306549E-5
30-31	1.2851939657572919E-4	3.059985632755457E-4	0.0	0.027252232045320103	3.671982759306549E-5
32-33	1.5299928163777285E-4	3.182385058065675E-4	0.0	0.02965126078140038	3.671982759306549E-5
34-35	1.5911925290328376E-4	3.916781609926985E-4	0.0	0.03285200575326259	4.8959770124087314E-5
36-37	1.6523922416879466E-4	4.5287787364780766E-4	0.0	0.03736854454720964	4.8959770124087314E-5
38-39	1.713591954343056E-4	4.5287787364780766E-4	0.0	0.045526466244135685	4.8959770124087314E-5
40-41	1.713591954343056E-4	5.752772989580259E-4	0.0	0.0639842995809166	4.8959770124087314E-5
42-43	1.713591954343056E-4	5.936372127545587E-4	0.0	0.07242373995605615	4.8959770124087314E-5
44-45	1.713591954343056E-4	6.548369254096678E-4	0.0	0.0839415258777477	4.8959770124087314E-5
46-47	1.713591954343056E-4	7.099166667992661E-4	0.0	0.09736262286301313	4.8959770124087314E-5
48-49	1.713591954343056E-4	7.16036638064777E-4	0.0	0.10915580749165266	4.8959770124087314E-5
50-51	1.7747916669981652E-4	8.017162357819297E-4	0.0	0.12068583335587524	4.8959770124087314E-5
52-53	1.8359913796532742E-4	8.139561783129515E-4	0.0	0.13733827516933042	5.5079741389598224E-5
54-55	1.8359913796532742E-4	8.261961208439735E-4	0.0	0.17194059270452913	6.119971265510914E-5
56-57	1.8359913796532742E-4	8.56795977171528E-4	0.0	0.20902149860225977	6.119971265510914E-5
58-59	1.8359913796532742E-4	8.56795977171528E-4	0.0	0.23812196196976415	7.343965518613097E-5
60-61	1.8971910923083833E-4	8.690359197025498E-4	0.0	0.5664890202207522	7.343965518613097E-5
62-63	1.9583908049634926E-4	8.812758622335716E-4	0.0	0.573135309015097	7.343965518613097E-5
64-65	1.9583908049634926E-4	0.001077114942729921	0.0	0.5802711955106828	7.343965518613097E-5
66-67	2.14198994292882E-4	0.0010832349139954318	0.0	0.5866971653394693	7.343965518613097E-5
68-69	2.3255890808941473E-4	0.001132194684119519	0.0	0.5929089361739629	7.343965518613097E-5
70-71	2.4479885062043657E-4	0.0011750344829780956	0.0	0.5989493478130221	9.179956898266371E-5
72-73	2.4479885062043657E-4	0.0011933943967746283	0.0	0.603092568359773	1.2239942531021828E-4
74-75	2.4479885062043657E-4	0.0012239942531021828	0.0	0.606733951262752	1.2239942531021828E-4
76-77	2.5091882188594747E-4	0.0012851939657572919	0.0	0.610014255861066	1.2851939657572919E-4
78-79	2.5703879315145837E-4	0.0013219137933503575	0.0	0.6130375216662283	1.3463936784124012E-4
80-81	2.5703879315145837E-4	0.0013280337646158684	0.0	0.6142064361779409	1.4075933910675105E-4
82-83	2.5703879315145837E-4	0.0013341537358813793	0.0	0.6146960338791818	1.5911925290328376E-4
84-85	2.5703879315145837E-4	0.0013341537358813793	0.0	0.6150265123275194	1.5911925290328376E-4
86-87	2.5703879315145837E-4	0.001346393678412401	0.0	0.6151305518390331	1.6523922416879466E-4
88-89	2.5703879315145837E-4	0.0014075933910675104	0.0	0.6151427917815641	1.8359913796532742E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11150	0.0	53.904125	1
GTATCAA	21095	0.0	39.262363	1
TCAACGC	27175	0.0	30.05022	4
ATCAACG	27345	0.0	29.89439	3
TATCAAC	28050	0.0	29.404991	2
CAACGCA	27930	0.0	29.192043	5
AACGCAG	28690	0.0	28.50839	6
ACGCAGA	32755	0.0	24.944237	7
CGAGACC	5775	0.0	24.627966	88-89
CGCAGAG	33460	0.0	24.39304	8
GAGACCG	5860	0.0	24.319567	88-89
AGACCGA	6040	0.0	23.601387	90-91
GACCGAG	6165	0.0	23.405989	90-91
ACCGAGG	6410	0.0	22.909727	92-93
TGGTATC	5250	0.0	22.863754	2
ACGAGAC	6325	0.0	22.848118	86-87
ATCTCCG	6075	0.0	22.580997	74-75
GTGGTAT	5570	0.0	22.473799	1
CCCACGA	6325	0.0	22.04338	84-85
GCCCACG	6440	0.0	21.666325	82-83
>>END_MODULE