FastQCFastQC Report
Fri 27 May 2016
SRR2139630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8656330
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.052.1014981
GTATCAA202050.038.4689251
TATCTCG86450.034.6633594-95
TATGCCG38750.031.55935988-89
CCGTCTT42150.030.81966692-93
TCAACGC254150.030.0683754
ATCAACG256550.029.7867433
CAACGCA261600.029.2125745
TATCAAC265050.029.0632292
CTCGTAT41350.029.00413784-85
AACGCAG266850.028.6215866
TGCCGTC44350.028.57255690-91
GCCGTCT43550.027.97829290-91
TCTCGTA41900.027.67277582-83
ATGCCGT43950.027.18423588-89
TCGTATG43950.026.85219284-85
CGTATGC45600.026.84948286-87
GCTATCT141400.026.81027294-95
ACGCAGA308100.024.7191567
CGCAGAG315350.024.1508548
Produced by FastQC (version 0.11.5)