FastQCFastQC Report
Fri 27 May 2016
SRR2139629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6788545
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88650.054.752851
GTATCAA164650.038.8859371
TATCTCG69300.036.38603694-95
ATCAACG198800.031.6375063
TCAACGC205400.030.6840954
CAACGCA211350.029.8002365
TATCAAC214450.029.6332112
AACGCAG214100.029.4376726
ACGCAGA220000.028.6088837
GCCGTCT32800.025.769190-91
ATGCCGT33650.024.64430688-89
TCGTATG33550.024.56958484-85
ATCTCGT55800.024.26443994-95
TCTCGTA33750.024.18841482-83
CGCAGAG261050.024.110158
CTATCTC112800.022.79250594-95
GTACATG214400.021.6101251
TATGCCG29800.021.3917688-89
GCTATCT110750.021.31419292-93
ACATGGG208400.021.2962843
Produced by FastQC (version 0.11.5)