##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6788545
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0712971630887	34.0	33.0	34.0	31.0	34.0
2	33.20630252874511	34.0	33.0	34.0	31.0	34.0
3	33.843506966514916	34.0	34.0	34.0	31.0	37.0
4	36.49142798640946	37.0	37.0	37.0	35.0	37.0
5	36.47234893486012	37.0	37.0	37.0	35.0	37.0
6	36.50567433816819	37.0	37.0	37.0	35.0	37.0
7	36.53314060671322	37.0	37.0	37.0	35.0	37.0
8	36.819333156073945	37.0	37.0	37.0	35.0	39.0
9	38.25571783055132	39.0	39.0	39.0	37.0	39.0
10-11	38.21951147116208	39.0	39.0	39.0	37.0	39.0
12-13	38.372756754208744	39.0	39.0	39.0	37.0	40.0
14-15	39.709350972852064	41.0	40.0	41.0	37.0	41.0
16-17	39.6790474983962	41.0	40.0	41.0	37.0	41.0
18-19	39.69043020264284	41.0	40.0	41.0	37.0	41.0
20-21	39.680847435794846	41.0	40.0	41.0	37.0	41.0
22-23	39.628062291618946	41.0	40.0	41.0	37.0	41.0
24-25	39.557902261325616	41.0	40.0	41.0	37.0	41.0
26-27	39.50041866895748	41.0	40.0	41.0	37.0	41.0
28-29	39.34332814502555	41.0	39.5	41.0	36.0	41.0
30-31	39.260696155732944	41.0	39.0	41.0	36.0	41.0
32-33	39.1556974393483	41.0	39.0	41.0	35.5	41.0
34-35	39.071415408262425	40.5	39.0	41.0	35.0	41.0
36-37	39.17264507091011	41.0	39.0	41.0	35.0	41.0
38-39	39.18712053689072	41.0	39.0	41.0	35.0	41.0
40-41	39.17985820040867	41.0	39.0	41.0	35.0	41.0
42-43	39.13079017421427	41.0	39.0	41.0	35.0	41.0
44-45	39.06215228528548	40.5	39.0	41.0	35.0	41.0
46-47	38.97513840740385	40.0	39.0	41.0	35.0	41.0
48-49	38.89896106188293	40.0	39.0	41.0	35.0	41.0
50-51	38.80440429692833	40.0	38.0	41.0	35.0	41.0
52-53	38.69821658358132	40.0	38.0	41.0	35.0	41.0
54-55	38.57422062830635	40.0	38.0	41.0	35.0	41.0
56-57	38.40452132160351	40.0	37.5	41.0	34.5	41.0
58-59	38.243272154805624	40.0	37.0	41.0	34.0	41.0
60-61	38.04558273822781	40.0	36.5	41.0	34.0	41.0
62-63	37.7887882271596	39.0	36.0	41.0	34.0	41.0
64-65	37.49432965829746	39.0	35.5	40.5	33.5	41.0
66-67	37.17313983542897	38.0	35.0	40.0	33.0	41.0
68-69	36.865223951592306	37.5	35.0	40.0	33.0	41.0
70-71	36.531997236662036	37.0	35.0	39.5	33.0	41.0
72-73	36.182248631908806	36.5	35.0	39.0	32.5	40.5
74-75	35.723733162833	36.0	34.5	38.5	32.0	40.0
76-77	34.94272678579818	35.0	34.0	37.0	31.0	39.0
78-79	35.08630033524845	35.0	34.0	37.0	31.5	39.0
80-81	34.872170144486766	35.0	34.0	37.0	32.0	39.0
82-83	34.59914259913848	35.0	34.0	36.0	31.0	37.5
84-85	34.35712326402695	35.0	34.0	36.0	31.0	37.0
86-87	34.10905404218044	35.0	34.0	35.0	31.0	37.0
88-89	33.842802418577804	35.0	34.0	35.0	31.0	36.0
90-91	33.77849065534684	35.0	34.0	35.0	31.0	36.0
92-93	33.701144543608095	35.0	34.0	35.0	31.0	36.0
94-95	33.59884471523142	35.0	33.5	35.0	31.0	35.5
96-97	33.553448480713996	35.0	33.0	35.0	31.0	35.0
98-99	33.517924992296244	34.5	33.0	35.0	30.5	35.0
100-101	32.263518717656915	33.5	31.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	31.0
21	149.0
22	556.0
23	1904.0
24	4874.0
25	10047.0
26	17863.0
27	28356.0
28	40983.0
29	54905.0
30	71161.0
31	90036.0
32	114413.0
33	151320.0
34	221929.0
35	340231.0
36	647374.0
37	1564471.0
38	2335549.0
39	1022262.0
40	70127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.004989096846494	0.0033439381839881926	25.571036368936845	30.420630596032677
2	14.330154233154898	24.251719209568705	38.016591502960836	23.401535054315563
3	15.73123780298102	24.929926063674333	33.38514120259072	25.95369493075393
4	12.751410722448101	23.463635716824655	37.35287527824453	26.432078282482706
5	17.35892114715615	36.500286364793396	32.139424889932215	14.001367598118241
6	27.979519614220667	37.318193709873576	20.34935620952004	14.352930466385722
7	26.246187695034457	32.88774441176193	22.350374395452103	18.515693497751506
8	24.364554273951608	31.855441124176593	22.18623675010039	21.59376785177141
9	23.798995540416286	20.497174202470465	23.749632836347683	31.954197420765567
10-11	24.565318153150045	26.082000889887375	28.670290098717693	20.68239085824489
12-13	24.91128069416937	24.2442084423098	28.48703956444275	22.357471299078078
14-15	22.396521610012634	26.678103092224646	26.5902418236137	24.335133474149018
16-17	21.54471889035568	28.886473346512098	27.531811222466175	22.03699654066605
18-19	21.646026010879503	27.946360810212028	29.28983740843397	21.11777577047449
20-21	22.367126754515347	27.253662601998784	29.192049204688452	21.187161438797414
22-23	22.27824238764843	26.93347590214168	28.96975890761021	21.81852280259968
24-25	21.901404440014698	27.233130942040834	29.17797935781219	21.68748526013228
26-27	21.810714638581132	27.48066342338144	29.01761342773171	21.69100851030571
28-29	21.86877328635513	27.264340902175704	29.057516039244796	21.809369772224375
30-31	22.001494858867517	27.219799503461562	29.031545639928314	21.747159997742603
32-33	21.814310992363385	27.566895597086422	28.883608536923035	21.73518487362716
34-35	22.03017774336805	27.32485284415102	28.837931407085332	21.807038005395604
36-37	21.926135384112467	27.378441287757898	28.888563450453393	21.806859877676242
38-39	21.87112426113574	27.63044972364558	28.80948397048381	21.688942044734873
40-41	22.107132307708984	27.300744422252993	28.619577435059902	21.97254583497812
42-43	21.996610262244975	27.389887527437118	28.61355693485302	21.999945275464892
44-45	22.04798901762889	27.645680946373417	28.26130674776051	22.045023288237175
46-47	22.21253662844901	27.407442971853392	28.04105473877689	22.338965660920714
48-49	21.979470280823097	27.73929054955357	28.077291801202282	22.203947368421055
50-51	22.095964684023485	27.964587560934206	27.780727426604606	22.1587203284377
52-53	22.281273960236778	27.631175053248043	27.539608401135695	22.547942585379488
54-55	22.19812472290358	27.423213403642716	27.847266310111884	22.53139556334182
56-57	22.107752352383798	27.42726548367646	27.950360521502084	22.514621642437664
58-59	22.079259302363006	27.00526199876675	28.099702881866722	22.815775817003516
60-61	22.062713791500013	27.046491441893878	28.390277683937587	22.500517082668527
62-63	21.821191720841814	27.584657068607942	28.30687467676465	22.28727653378559
64-65	21.643838987155544	27.52609717908936	28.094881644007348	22.735182189747746
66-67	21.621186587819547	28.11367057838241	27.70264423813463	22.562498595663406
68-69	21.695830313001913	28.43608786183639	27.389356854031043	22.47872497113065
70-71	21.76993702310514	27.842388163194045	27.38075206405283	23.006922749647988
72-73	21.658125369797894	27.558092461467375	27.36374814943244	23.420034019302292
74-75	21.998607804100406	27.498835657385847	27.371593134361017	23.130963404152734
76-77	22.423547598219827	27.162472887323673	27.14033535865673	23.273644155799772
78-79	22.407767077925424	27.185996499524123	26.75287206182473	23.653364360725725
80-81	22.386953689064374	27.64160275545995	26.55059563091664	23.420847924559034
82-83	22.42947864142327	27.49679795296186	26.64212296428617	23.4316004413287
84-85	22.421527118636767	27.600855754498472	26.710234591177812	23.267382535686952
86-87	22.736728956101928	27.863324090572338	26.563744132699135	22.836202820626603
88-89	22.84335536550543	27.540178355153618	26.872291080229115	22.744175199111837
90-91	22.614580377886153	27.47690769205835	27.076798748414888	22.831713181640612
92-93	22.43232477119632	27.172214471608903	27.059747924172267	23.335712833022505
94-95	22.297651642781823	27.025406199336256	27.593372315837488	23.083569842044433
96-97	21.965050681798125	27.282322637159723	27.87356712163524	22.87905955940691
98-99	22.42910386611045	27.92850689537	27.96672208727876	21.675667151240795
100-101	22.96772694324599	30.17540909964659	24.940195261112386	21.91666869599504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1770.0
1	1502.5
2	1788.5
3	2501.5
4	3424.5
5	5329.5
6	6920.5
7	8440.5
8	9732.5
9	10015.0
10	9364.0
11	8559.0
12	9342.0
13	12141.5
14	19225.5
15	29745.0
16	38827.0
17	43858.0
18	44228.5
19	40947.0
20	36134.0
21	31488.5
22	28569.0
23	28928.0
24	32965.0
25	40475.5
26	50682.5
27	62309.0
28	75973.5
29	90933.0
30	106008.0
31	121475.5
32	137355.0
33	153039.5
34	168671.0
35	183966.0
36	199439.5
37	213033.0
38	224070.5
39	234812.0
40	244975.5
41	253715.0
42	258635.0
43	263249.5
44	268749.5
45	272621.5
46	274705.0
47	274331.0
48	273238.5
49	271910.5
50	268850.0
51	262410.5
52	252801.5
53	240526.5
54	226172.0
55	211140.0
56	193619.5
57	174500.0
58	156085.0
59	136139.5
60	114246.0
61	93561.0
62	74808.0
63	57703.5
64	43069.0
65	32069.5
66	24225.0
67	18507.0
68	14286.0
69	10679.5
70	7732.5
71	5645.5
72	4010.5
73	2813.0
74	1999.5
75	1299.5
76	825.0
77	514.5
78	322.0
79	203.0
80	126.0
81	78.0
82	55.5
83	44.0
84	34.0
85	23.5
86	17.0
87	17.0
88	14.0
89	8.5
90	3.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0020917589851728166
2	0.003697404966749134
3	1.473069707868181E-5
4	2.946139415736362E-5
5	1.473069707868181E-5
6	1.473069707868181E-5
7	1.473069707868181E-5
8	4.419209123604543E-5
9	2.7988324449495437E-4
10-11	6.702467170800223E-4
12-13	0.0
14-15	2.946139415736362E-5
16-17	0.00146570435932884
18-19	1.473069707868181E-5
20-21	5.155868534780237E-5
22-23	9.649524838245237E-4
24-25	0.00514234231924501
26-27	0.05334711313560093
28-29	0.3628764135934408
30-31	0.33710637676595917
32-33	0.33003166064182715
34-35	0.15999755447996863
36-37	0.052399765419568145
38-39	0.04784284946466586
40-41	0.030236866491366088
42-43	0.02836230461039377
44-45	0.01658393169865697
46-47	0.012665227775361616
48-49	0.012519594489789131
50-51	0.010407131704961508
52-53	0.007888561396460143
54-55	0.0021427935480881617
56-57	0.0054626284172897095
58-59	0.005105243322714322
60-61	0.007042492249677596
62-63	0.0022548229137511777
64-65	4.208943616667546E-4
66-67	8.088913335382525E-4
68-69	0.0018400132414943943
70-71	0.004275228724736774
72-73	0.0021629815884818956
74-75	0.0013685460515746354
76-77	6.526148947843104E-4
78-79	3.211276196744998E-4
80-81	0.0010969626333281292
82-83	0.001100469687562456
84-85	4.128339669736416E-4
86-87	0.0033595703672455327
88-89	0.002215394435811658
90-91	0.006193829995293621
92-93	0.012731866605928987
94-95	0.0011219194680452402
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	98.0
20-21	391.0
22-23	775.0
24-25	1892.0
26-27	2673.0
28-29	5813.0
30-31	13160.0
32-33	18602.0
34-35	27619.0
36-37	35642.0
38-39	27252.0
40-41	43795.0
42-43	52875.0
44-45	63679.0
46-47	62224.0
48-49	54397.0
50-51	43432.0
52-53	8747.0
54-55	7866.0
56-57	8087.0
58-59	13215.0
60-61	52156.0
62-63	52896.0
64-65	57012.0
66-67	59567.0
68-69	60093.0
70-71	59259.0
72-73	58374.0
74-75	59248.0
76-77	61087.0
78-79	63040.0
80-81	63615.0
82-83	62961.0
84-85	63925.0
86-87	66475.0
88-89	70165.0
90-91	74033.0
92-93	76734.0
94-95	88420.0
96-97	165601.0
98-99	723650.0
100-101	4258000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.70554566376137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.41314629005626	78.39321184256809
2	6.976285377302455	12.097652606902573
3	1.5604592506570285	4.0590141244284546
4	0.5135421939863136	1.7810782460379406
5	0.21892343849544352	0.9490938096667165
6	0.10864644471011782	0.5652149563811066
7	0.06113176181162974	0.37103239356851114
8	0.03769621333655318	0.2614776597442721
9	0.027207867937134523	0.21231657322531255
>10	0.0805106263731605	1.0882708427171044
>50	0.0019038604371329928	0.10693132145213786
>100	4.962538364070692E-4	0.08578631053629972
>500	5.042106033500846E-5	0.028919312771446475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.365348539340904E-5	0.0	0.0	0.0
3	0.0	7.365348539340904E-5	0.0	0.0	0.0
4	0.0	7.365348539340904E-5	0.0	0.0	0.0
5	0.0	8.838418247209085E-5	0.0	0.0	0.0
6	2.9461394157363616E-5	8.838418247209085E-5	0.0	0.0	0.0
7	2.9461394157363616E-5	8.838418247209085E-5	0.0	2.9461394157363616E-5	0.0
8	4.4192091236045425E-5	1.0311487955077266E-4	0.0	2.9461394157363616E-5	0.0
9	4.4192091236045425E-5	1.9149906202286352E-4	0.0	2.9461394157363616E-5	0.0
10-11	4.4192091236045425E-5	1.9149906202286352E-4	0.0	4.4192091236045425E-5	0.0
12-13	4.4192091236045425E-5	1.9149906202286352E-4	0.0	6.628813685406813E-5	0.0
14-15	4.4192091236045425E-5	3.314406842703407E-4	0.0	1.1784557662945447E-4	1.4730697078681808E-5
16-17	5.892278831472723E-5	3.8299812404572704E-4	0.0	1.473069707868181E-4	2.9461394157363616E-5
18-19	5.892278831472723E-5	3.8299812404572704E-4	0.0	4.5665160943913607E-4	2.9461394157363616E-5
20-21	5.892278831472723E-5	3.8299812404572704E-4	0.0	0.0012079171604519082	2.9461394157363616E-5
22-23	8.838418247209085E-5	4.271902152817725E-4	0.0	0.0018928945746106123	2.9461394157363616E-5
24-25	8.838418247209085E-5	4.934783521358406E-4	0.0	0.0029461394157363615	4.4192091236045425E-5
26-27	8.838418247209085E-5	5.303050948325451E-4	0.0	0.007372713887880245	4.4192091236045425E-5
28-29	8.838418247209085E-5	5.303050948325451E-4	0.0	0.024423495756454437	4.4192091236045425E-5
30-31	8.838418247209085E-5	6.481506714619996E-4	0.0	0.03254747519534745	4.4192091236045425E-5
32-33	8.838418247209085E-5	6.702467170800223E-4	0.0	0.03662787828614232	4.4192091236045425E-5
34-35	9.574953101143175E-5	7.65996248091454E-4	0.0	0.04218135108480536	7.365348539340904E-5
36-37	1.0311487955077266E-4	8.691111276422268E-4	0.0	0.05186678441403865	7.365348539340904E-5
38-39	1.0311487955077266E-4	9.574953101143176E-4	0.0	0.09578635775412847	7.365348539340904E-5
40-41	1.0311487955077266E-4	0.0010237834469683858	0.0	0.2984439228140935	7.365348539340904E-5
42-43	1.0311487955077266E-4	0.001163725069215863	0.0	0.317792693426942	7.365348539340904E-5
44-45	1.0311487955077266E-4	0.0012815706458453174	0.0	0.3473792985094744	7.365348539340904E-5
46-47	1.0311487955077266E-4	0.0012815706458453174	0.0	0.3792859883818992	7.365348539340904E-5
48-49	1.0311487955077266E-4	0.0012815706458453174	0.0	0.4079887516397107	7.365348539340904E-5
50-51	1.1048022809011357E-4	0.0013404934341600446	0.0	0.4440715941339418	7.365348539340904E-5
52-53	1.1784557662945447E-4	0.0013404934341600446	0.0	0.677177510055542	7.365348539340904E-5
54-55	1.1784557662945447E-4	0.0013552241312387264	0.0	1.2958667873601781	7.365348539340904E-5
56-57	1.1784557662945447E-4	0.00138468552539609	0.0	1.9698771975438034	7.365348539340904E-5
58-59	1.1784557662945447E-4	0.00138468552539609	0.0	2.6460530201979955	7.365348539340904E-5
60-61	1.1784557662945447E-4	0.0014509736622501583	0.0	3.0890713694908114	7.365348539340904E-5
62-63	1.1784557662945447E-4	0.0015025311020255446	0.0	3.098786264214202	7.365348539340904E-5
64-65	1.1784557662945447E-4	0.001679299466969726	0.0	3.1059306522973626	7.365348539340904E-5
66-67	1.4730697078681808E-4	0.0016940301640484081	0.0	3.1124489857546793	7.365348539340904E-5
68-69	1.4730697078681808E-4	0.0017161262096664308	0.0	3.118488571556939	7.365348539340904E-5
70-71	1.4730697078681808E-4	0.0017234915582057717	0.0	3.123555931352006	7.365348539340904E-5
72-73	1.4730697078681808E-4	0.0017308569067451126	0.0	3.127673161185497	8.838418247209085E-5
74-75	1.4730697078681808E-4	0.001775048997981158	0.0	3.1309728373311216	8.838418247209085E-5
76-77	1.4730697078681808E-4	0.0018707985289925897	0.0	3.134397724401915	8.838418247209085E-5
78-79	1.4730697078681808E-4	0.0019149906202286353	0.0	3.137822611472709	8.838418247209085E-5
80-81	1.4730697078681808E-4	0.0019149906202286353	0.0	3.1389863365419246	8.838418247209085E-5
82-83	1.4730697078681808E-4	0.0019223559687679762	0.0	3.1393914307115884	8.838418247209085E-5
84-85	1.4730697078681808E-4	0.0019444520143859988	0.0	3.1396786793046227	8.838418247209085E-5
86-87	1.4730697078681808E-4	0.0019591827114646804	0.0	3.13981862092687	8.838418247209085E-5
88-89	1.4730697078681808E-4	0.0020181054997794076	0.0	3.139877543715185	8.838418247209085E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8865	0.0	54.75285	1
GTATCAA	16465	0.0	38.885937	1
TATCTCG	6930	0.0	36.386036	94-95
ATCAACG	19880	0.0	31.637506	3
TCAACGC	20540	0.0	30.684095	4
CAACGCA	21135	0.0	29.800236	5
TATCAAC	21445	0.0	29.633211	2
AACGCAG	21410	0.0	29.437672	6
ACGCAGA	22000	0.0	28.608883	7
GCCGTCT	3280	0.0	25.7691	90-91
ATGCCGT	3365	0.0	24.644306	88-89
TCGTATG	3355	0.0	24.569584	84-85
ATCTCGT	5580	0.0	24.264439	94-95
TCTCGTA	3375	0.0	24.188414	82-83
CGCAGAG	26105	0.0	24.11015	8
CTATCTC	11280	0.0	22.792505	94-95
GTACATG	21440	0.0	21.610125	1
TATGCCG	2980	0.0	21.39176	88-89
GCTATCT	11075	0.0	21.314192	92-93
ACATGGG	20840	0.0	21.296284	3
>>END_MODULE