FastQCFastQC Report
Fri 27 May 2016
SRR2139628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6912038
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83350.053.529941
GTATCAA160200.039.5634461
TATCTCG84000.030.50983494-95
TCAACGC203250.030.5015854
ATCAACG205200.030.2536473
TATCAAC210150.029.9539872
CAACGCA208000.029.8466055
AACGCAG214450.028.9690696
ACGCAGA245500.025.287557
CGCAGAG253850.024.4046638
CCGTCTT47600.022.24572492-93
TATGCCG45050.022.17885488-89
GCTATCT128700.022.15308694-95
TGCCGTC47850.021.61488790-91
GTACATG223200.021.5587541
CTATCTC130200.021.47792494-95
GCAGAGT288150.021.3496179
CTCGTAT46950.021.07384584-85
TACATGG224950.020.8913362
GTGGTAT42550.020.5245881
Produced by FastQC (version 0.11.5)