FastQCFastQC Report
Fri 27 May 2016
SRR2139627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7322062
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103250.055.0306321
GTATCAA188900.036.7733461
TATCTCG84500.032.14515794-95
TCAACGC234950.028.9196744
ATCAACG237650.028.554473
TATCAAC241800.028.2446462
CAACGCA240650.028.1988895
AACGCAG249000.027.3054396
GTGGTAT46700.025.5021341
TGGTATC46100.024.049152
ACGCAGA286300.023.7177567
CGCAGAG291700.023.2489958
GCTATCT128200.022.73558894-95
CTATCTC130800.021.5642894-95
ATCTCGT69200.020.23419694-95
GCAGAGT334600.020.086989
GTACATG232600.020.0524181
TACATGG229450.019.6859782
GAGTACT198700.019.66972212-13
CGCTATC127800.019.43015592-93
Produced by FastQC (version 0.11.5)