##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7322062
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94609865363063	34.0	31.0	34.0	31.0	34.0
2	33.07810313542824	34.0	33.0	34.0	31.0	34.0
3	33.1352815641277	34.0	33.0	34.0	31.0	34.0
4	36.48480810460223	37.0	37.0	37.0	35.0	37.0
5	36.45113029635641	37.0	37.0	37.0	35.0	37.0
6	36.47983464220871	37.0	37.0	37.0	35.0	37.0
7	36.45511209820403	37.0	37.0	37.0	35.0	37.0
8	36.4427385345822	37.0	37.0	37.0	35.0	37.0
9	38.24067755776993	39.0	39.0	39.0	37.0	39.0
10-11	38.18308073600032	39.0	39.0	39.0	37.0	39.0
12-13	38.15574793002299	39.0	39.0	39.0	37.0	39.0
14-15	39.67264856539046	41.0	40.0	41.0	37.0	41.0
16-17	39.64257390882514	41.0	40.0	41.0	37.0	41.0
18-19	39.653788236155336	41.0	40.0	41.0	37.0	41.0
20-21	39.64727812511303	41.0	40.0	41.0	37.0	41.0
22-23	39.59111338611185	41.0	40.0	41.0	37.0	41.0
24-25	39.52661652977831	41.0	40.0	41.0	37.0	41.0
26-27	39.47543958202198	41.0	39.5	41.0	36.5	41.0
28-29	39.30267472399497	41.0	39.0	41.0	36.0	41.0
30-31	39.217176083806294	41.0	39.0	41.0	36.0	41.0
32-33	39.11219294603532	41.0	39.0	41.0	35.0	41.0
34-35	39.01460520280978	40.5	39.0	41.0	35.0	41.0
36-37	39.113018268537196	40.5	39.0	41.0	35.0	41.0
38-39	39.13746765313343	41.0	39.0	41.0	35.0	41.0
40-41	39.132476168858474	41.0	39.0	41.0	35.0	41.0
42-43	39.09077870782856	41.0	39.0	41.0	35.0	41.0
44-45	39.025139328440424	40.0	39.0	41.0	35.0	41.0
46-47	38.93906389046428	40.0	39.0	41.0	35.0	41.0
48-49	38.86365983092022	40.0	38.0	41.0	35.0	41.0
50-51	38.76982830818769	40.0	38.0	41.0	35.0	41.0
52-53	38.66507410318951	40.0	38.0	41.0	35.0	41.0
54-55	38.5484429436935	40.0	38.0	41.0	35.0	41.0
56-57	38.37666171981856	40.0	37.5	41.0	34.0	41.0
58-59	38.22029007525585	40.0	37.0	41.0	34.0	41.0
60-61	38.0301404821992	40.0	36.5	41.0	34.0	41.0
62-63	37.7843273319178	39.0	36.0	41.0	34.0	41.0
64-65	37.495619962121424	39.0	35.5	40.5	33.5	41.0
66-67	37.17775492149269	38.5	35.0	40.0	33.0	41.0
68-69	36.87126454349706	37.5	35.0	40.0	33.0	41.0
70-71	36.54710459048767	37.0	35.0	39.5	33.0	41.0
72-73	36.2072992855132	36.5	35.0	39.0	32.0	41.0
74-75	35.83244770538211	36.0	35.0	39.0	32.0	40.0
76-77	34.88159757384304	35.0	34.0	37.0	30.5	39.0
78-79	35.08826994197427	35.0	34.0	37.0	31.0	39.0
80-81	34.88964620048576	35.0	34.0	37.0	31.0	39.0
82-83	34.61307626835752	35.0	34.0	36.0	31.0	38.5
84-85	34.36581118103608	35.0	34.0	36.0	31.0	37.0
86-87	34.12485079539421	35.0	34.0	35.5	31.0	37.0
88-89	33.81902130578189	35.0	34.0	35.0	31.0	36.5
90-91	33.75577022572081	35.0	34.0	35.0	31.0	36.0
92-93	33.6802247219141	35.0	34.0	35.0	31.0	36.0
94-95	33.573354343458995	35.0	33.5	35.0	30.5	35.5
96-97	33.523888768014025	35.0	33.0	35.0	30.0	35.0
98-99	33.51547324047304	34.5	33.0	35.0	30.0	35.0
100-101	32.43619454472774	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.0
20	26.0
21	165.0
22	621.0
23	2082.0
24	5469.0
25	11297.0
26	20102.0
27	31907.0
28	45672.0
29	61406.0
30	79403.0
31	100151.0
32	127909.0
33	168284.0
34	245761.0
35	376025.0
36	713329.0
37	1715072.0
38	2512653.0
39	1050520.0
40	54200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.42568363939011	21.19204945273613	15.299720215425655	24.082546692448112
2	13.943149831862062	21.643950756023184	41.18439147806094	23.228507934053816
3	16.96138328246879	26.669796021940268	31.33335937335685	25.03546132223409
4	12.093587844517023	18.732851483639447	39.01033615940428	30.163224512439257
5	13.010010021767094	37.892345079842265	35.737036916649984	13.360607981740664
6	29.377639796002818	38.28013201745629	18.8479010420835	13.494327144457394
7	25.922397270058628	32.99922890573721	23.385570895193183	17.69280292901098
8	24.418353736966445	36.20156453195835	21.400447032543564	17.979634698531644
9	25.454746839030264	16.769654799802407	21.54781706357558	36.22778129759175
10-11	23.873807832073375	26.964169321050274	29.095094480577906	20.066928366298452
12-13	24.794600209613087	24.664589018776407	29.07107314852018	21.469737623090328
14-15	22.04951965716761	25.96789674821109	26.882188378082567	25.10039521653873
16-17	21.475667102047073	29.01729928620786	27.558357238055248	21.94867637368982
18-19	21.520467868204342	28.10063613228077	29.38151848482026	20.99737751469463
20-21	22.368036032204508	27.234638521491945	29.297670159000518	21.099655287303033
22-23	22.120195311627654	27.132675633667702	29.134833576242443	21.612295478462197
24-25	21.856742767052573	27.315434916741673	29.223253109973236	21.604569206232522
26-27	21.777392007364227	27.489225881498907	29.149614023080993	21.583768088055873
28-29	21.705406732096165	27.423184489292158	29.24592111893562	21.62548765967606
30-31	21.91764353713847	27.29029153009704	29.1899126824037	21.60215225036079
32-33	21.71838994500625	27.548254550052814	29.011264176539203	21.72209132840173
34-35	21.88421569387386	27.481914408013157	29.01235907638512	21.62151082172786
36-37	21.843455884664724	27.43968222544456	29.026447469388078	21.690414420502634
38-39	21.74064544002439	27.614956067702785	28.9686004122077	21.67579808006512
40-41	21.90655292992551	27.4191041731916	28.911351329482432	21.76299156740046
42-43	21.962528759126446	27.51323420280632	28.664884663215744	21.859352374851486
44-45	21.904049718721424	27.650726236991414	28.465991037081	21.979233007206165
46-47	22.05760100959516	27.564015430093836	28.156766262122723	22.22161729818828
48-49	21.98786855197677	27.808213251624007	28.113626496632154	22.090291699767064
50-51	21.961833207413825	27.923841493194157	27.978360488687127	22.13596481070489
52-53	22.11411151064008	27.803808953542337	27.741578860907207	22.340500674910377
54-55	22.116612962006275	27.58944766339787	27.843975249851955	22.449964124743904
56-57	22.151116570980175	27.404980889641905	28.025659751866584	22.418242787511332
58-59	21.87376123624025	27.24476868316929	28.27272740649403	22.60874267409643
60-61	22.017051325807454	27.144199644057927	28.382948024663218	22.4558010054714
62-63	21.79526847163528	27.651303875758	28.36887553316278	22.18455211944395
64-65	21.468052773950948	27.77548138649888	28.237986190955883	22.518479648594298
66-67	21.56746962680541	28.3066529158684	27.7048367636366	22.42104069368959
68-69	21.599511833514377	28.481409734045855	27.48269991023195	22.43637852220782
70-71	21.61865339136938	28.052918703365826	27.491542605779006	22.836885299485786
72-73	21.551581222469267	27.768896695791558	27.372084058873792	23.30743802286539
74-75	21.88939879137964	27.51631479443774	27.40728214509371	23.187004269088913
76-77	22.247259685722266	27.34304434941456	27.335979111804633	23.073716853058546
78-79	22.41391776278256	27.222802954780168	26.796815853337137	23.56646342910014
80-81	22.31995043837732	27.617212037937723	26.673388269953495	23.389449253731463
82-83	22.34351105653641	27.641617566633304	26.76596052047755	23.248910856352737
84-85	22.325086474712148	27.64426436590638	26.72068187909153	23.309967280289946
86-87	22.591016726617504	27.907802913376706	26.71422125344154	22.78695910656425
88-89	22.779168146571106	27.628917640666202	26.916767094863758	22.675147117898938
90-91	22.529030240135086	27.676404400094007	27.149777391986113	22.644787967784797
92-93	22.396376460144683	27.22116261509086	27.098338654034382	23.28412227073007
94-95	22.22051642283157	27.147687218673266	27.64091217027369	22.990884188221475
96-97	21.921234286619185	27.38681489786768	27.86155099769674	22.830399817816403
98-99	22.154206601242965	27.590858152535812	28.149737975887295	22.105197270333925
100-101	22.84482144993325	30.475931655407713	25.4238602630034	21.255386631655643
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2722.0
1	2260.5
2	2409.0
3	3185.5
4	4218.5
5	6352.0
6	8129.5
7	9783.5
8	11051.5
9	11206.5
10	10462.5
11	9587.0
12	10640.5
13	14346.0
14	22898.0
15	34522.5
16	43910.5
17	48837.0
18	48959.0
19	45642.0
20	40691.5
21	35515.5
22	32176.0
23	32685.0
24	37199.0
25	45852.5
26	57801.5
27	71306.0
28	86960.5
29	103879.0
30	119412.5
31	135751.0
32	153413.0
33	171110.0
34	188057.0
35	203796.5
36	223046.0
37	238734.5
38	248814.5
39	258972.5
40	269274.5
41	277136.0
42	280219.0
43	284595.5
44	290914.5
45	295140.5
46	296791.0
47	296569.0
48	295121.5
49	292022.5
50	286437.5
51	278183.0
52	266233.5
53	250892.0
54	234434.0
55	218782.5
56	200541.0
57	180002.0
58	159953.0
59	138687.0
60	116479.5
61	94780.0
62	74667.5
63	57101.0
64	42417.5
65	31495.5
66	23903.0
67	18131.5
68	13752.5
69	10301.0
70	7529.5
71	5315.0
72	3655.0
73	2493.5
74	1663.0
75	1120.5
76	762.5
77	464.5
78	286.0
79	184.0
80	122.5
81	86.5
82	69.0
83	50.0
84	32.0
85	20.0
86	14.0
87	11.0
88	7.5
89	6.0
90	4.5
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005394655221438988
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.8680445481068037E-4
10-11	7.443258470086704E-4
12-13	0.0
14-15	0.0
16-17	0.0015364524364857878
18-19	0.0
20-21	0.0
22-23	8.263462683158625E-4
24-25	0.003032712103205652
26-27	0.008021182480808261
28-29	0.3696835560707802
30-31	0.3390883076765386
32-33	0.33805961297028814
34-35	0.19031998514575726
36-37	0.05056903284203835
38-39	0.04923139575486334
40-41	0.028800236538504453
42-43	0.028334042562155833
44-45	0.01675653414533354
46-47	0.011509719704346846
48-49	0.011655855872381233
50-51	0.010289528995171786
52-53	0.008579440305448589
54-55	0.0012913311254016792
56-57	0.004863683871715709
58-59	0.003950645040281498
60-61	0.007240251547446933
62-63	0.0022557380056342827
64-65	1.6524976262247167E-4
66-67	7.889325507425865E-4
68-69	0.0017631330414198281
70-71	0.004205670777767941
72-73	0.002221851044446018
74-75	0.0015250746772965688
76-77	7.344599843049094E-4
78-79	2.1787510285520482E-4
80-81	0.0012078500131027243
82-83	0.0014611130941158252
84-85	8.348993958751462E-6
86-87	0.0035904161402131
88-89	0.0017022888428310928
90-91	0.005088469147493309
92-93	0.01431231942565049
94-95	0.0015441213025993096
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	95.0
20-21	395.0
22-23	886.0
24-25	1974.0
26-27	2891.0
28-29	5179.0
30-31	14120.0
32-33	19172.0
34-35	28773.0
36-37	37789.0
38-39	29258.0
40-41	47985.0
42-43	56756.0
44-45	69432.0
46-47	68282.0
48-49	59555.0
50-51	52499.0
52-53	10012.0
54-55	9182.0
56-57	9155.0
58-59	9382.0
60-61	58597.0
62-63	58757.0
64-65	63453.0
66-67	69133.0
68-69	67222.0
70-71	65309.0
72-73	63298.0
74-75	64264.0
76-77	65818.0
78-79	69183.0
80-81	70185.0
82-83	67966.0
84-85	69832.0
86-87	72360.0
88-89	76332.0
90-91	80289.0
92-93	82795.0
94-95	90862.0
96-97	135337.0
98-99	669749.0
100-101	4728549.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.32682398161296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39021856865148	78.031004880355
2	6.879645663956774	11.877959205765265
3	1.5770528845061544	4.0842590031137345
4	0.5560991979443021	1.9202511030901563
5	0.23376411745922757	1.009005691055992
6	0.12274718595937187	0.6357824829931806
7	0.06819075442535281	0.4120683878115579
8	0.04451894667593522	0.3074543418832219
9	0.03151323025700628	0.24483933733397595
>10	0.09411688192682613	1.251743533761754
>50	0.0016340370566079398	0.09116162050479312
>100	3.7791173110078693E-4	0.05675025104067879
>500	9.649574434823872E-5	0.05533304033054338
>1k	2.4123705517791533E-5	0.022387120960200544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.82867749549239E-5	0.0	0.0	0.0
5	0.0	6.82867749549239E-5	0.0	0.0	0.0
6	2.731470998196956E-5	6.82867749549239E-5	0.0	0.0	0.0
7	2.731470998196956E-5	6.82867749549239E-5	0.0	0.0	0.0
8	2.731470998196956E-5	9.560148493689347E-5	0.0	0.0	1.365735499098478E-5
9	2.731470998196956E-5	1.9120296987378693E-4	0.0	1.365735499098478E-5	1.365735499098478E-5
10-11	2.731470998196956E-5	1.9120296987378693E-4	0.0	2.0486032486477172E-5	1.365735499098478E-5
12-13	2.731470998196956E-5	1.9120296987378693E-4	0.0	2.731470998196956E-5	1.365735499098478E-5
14-15	2.731470998196956E-5	2.116890023602641E-4	0.0	4.097206497295434E-5	1.365735499098478E-5
16-17	2.731470998196956E-5	2.1851767985575648E-4	0.0	6.82867749549239E-5	1.365735499098478E-5
18-19	4.097206497295434E-5	2.3900371234223365E-4	0.0	1.4340222740534018E-4	1.365735499098478E-5
20-21	4.097206497295434E-5	2.594897448287108E-4	0.0	1.9803164736927932E-4	2.0486032486477172E-5
22-23	4.097206497295434E-5	3.0729048729715753E-4	0.0	7.033537820357162E-4	2.731470998196956E-5
24-25	4.097206497295434E-5	3.550912297656043E-4	0.0	0.002151033411080103	2.731470998196956E-5
26-27	4.097206497295434E-5	3.687485847565891E-4	0.0	0.005421969931420958	2.731470998196956E-5
28-29	4.097206497295434E-5	3.8240593974757386E-4	0.0	0.020158255966693538	2.731470998196956E-5
30-31	4.097206497295434E-5	4.3703535971151297E-4	0.0	0.027813203439140503	2.731470998196956E-5
32-33	4.097206497295434E-5	4.3703535971151297E-4	0.0	0.03156214738416583	2.731470998196956E-5
34-35	4.780074246844673E-5	4.916647796754521E-4	0.0	0.03664951211830766	2.731470998196956E-5
36-37	5.462941996393912E-5	5.531228771348836E-4	0.0	0.04368987861616031	2.731470998196956E-5
38-39	5.462941996393912E-5	5.736089096213607E-4	0.0	0.06042696715761216	4.097206497295434E-5
40-41	6.82867749549239E-5	6.692103945582542E-4	0.0	0.2881428755997969	5.462941996393912E-5
42-43	6.82867749549239E-5	6.965251045402238E-4	0.0	0.30515993991856394	5.462941996393912E-5
44-45	7.511545245041629E-5	8.194412994590868E-4	0.0	0.3336218677197762	5.462941996393912E-5
46-47	8.194412994590867E-5	9.150427843959803E-4	0.0	0.3673145624825357	5.462941996393912E-5
48-49	8.194412994590867E-5	9.150427843959803E-4	0.0	0.3950253357592438	5.462941996393912E-5
50-51	8.877280744140108E-5	9.901582368463965E-4	0.0	0.422654164906006	5.462941996393912E-5
52-53	9.560148493689347E-5	9.96986914341889E-4	0.0	0.5857024974658778	5.462941996393912E-5
54-55	1.0243016243238585E-4	0.0010038155918373813	0.0	1.1900199697844678	5.462941996393912E-5
56-57	1.0925883992787824E-4	0.0010652736892968128	0.0	1.8571681037390833	5.462941996393912E-5
58-59	1.1608751742337063E-4	0.0010652736892968128	0.0	2.550818335053705	5.462941996393912E-5
60-61	1.2291619491886302E-4	0.0010721023667923052	0.0	3.074058919468314	5.462941996393912E-5
62-63	1.2291619491886302E-4	0.0010789310442877976	0.0	3.085100890978525	5.462941996393912E-5
64-65	1.365735499098478E-4	0.0012018472392066606	0.0	3.0926124362235665	5.462941996393912E-5
66-67	1.365735499098478E-4	0.0012155045941976454	0.0	3.0989357915843927	5.462941996393912E-5
68-69	1.4340222740534018E-4	0.001242819304179615	0.0	3.1047743108430383	5.462941996393912E-5
70-71	1.5705958239632496E-4	0.001242819304179615	0.0	3.109704615994784	6.82867749549239E-5
72-73	1.8437429237829454E-4	0.001263305336666092	0.0	3.114273001239268	8.194412994590867E-5
74-75	1.9120296987378693E-4	0.001331592111621016	0.0	3.1179400010543477	8.194412994590867E-5
76-77	1.9803164736927932E-4	0.001365735499098478	0.0	3.1212109375746886	8.877280744140108E-5
78-79	2.048603248647717E-4	0.001365735499098478	0.0	3.123921922540399	9.560148493689347E-5
80-81	2.048603248647717E-4	0.001365735499098478	0.0	3.125212542587047	9.560148493689347E-5
82-83	2.048603248647717E-4	0.001365735499098478	0.0	3.1257451794316955	9.560148493689347E-5
84-85	2.048603248647717E-4	0.0013793928540894627	0.0	3.1261344140489387	9.560148493689347E-5
86-87	2.048603248647717E-4	0.0013930502090804475	0.0	3.12622318685638	9.560148493689347E-5
88-89	2.116890023602641E-4	0.0014613369840353716	0.0	3.126250501566362	9.560148493689347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10325	0.0	55.030632	1
GTATCAA	18890	0.0	36.773346	1
TATCTCG	8450	0.0	32.145157	94-95
TCAACGC	23495	0.0	28.919674	4
ATCAACG	23765	0.0	28.55447	3
TATCAAC	24180	0.0	28.244646	2
CAACGCA	24065	0.0	28.198889	5
AACGCAG	24900	0.0	27.305439	6
GTGGTAT	4670	0.0	25.502134	1
TGGTATC	4610	0.0	24.04915	2
ACGCAGA	28630	0.0	23.717756	7
CGCAGAG	29170	0.0	23.248995	8
GCTATCT	12820	0.0	22.735588	94-95
CTATCTC	13080	0.0	21.56428	94-95
ATCTCGT	6920	0.0	20.234196	94-95
GCAGAGT	33460	0.0	20.08698	9
GTACATG	23260	0.0	20.052418	1
TACATGG	22945	0.0	19.685978	2
GAGTACT	19870	0.0	19.669722	12-13
CGCTATC	12780	0.0	19.430155	92-93
>>END_MODULE