FastQCFastQC Report
Fri 27 May 2016
SRR2139626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6183526
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.053.0226941
GTATCAA156300.038.3588181
ATCAACG196750.029.9491273
TCAACGC196700.029.8699634
TATCAAC202450.029.4243932
CAACGCA200400.029.318475
AACGCAG206450.028.5836626
ACGCAGA237400.024.785097
CGCAGAG243200.024.1764038
GTGGTAT38050.022.4937271
TGGTATC36350.021.5443152
GCAGAGT278000.021.027039
GAGTACT157000.020.08863412-13
GTACATG199850.020.0855961
AGAGTAC253050.019.37162610-11
TACATGG203500.019.3468152
ACATGGG203050.018.946183
CAGAGTA272050.018.60858210-11
GTACTTT175150.018.03166414-15
AGTACTT172150.017.79871212-13
Produced by FastQC (version 0.11.5)