##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6183526
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.915097631998314	34.0	31.0	34.0	31.0	34.0
2	33.04901944295213	34.0	33.0	34.0	31.0	34.0
3	33.10685424464941	34.0	33.0	34.0	31.0	34.0
4	36.46667952879959	37.0	37.0	37.0	35.0	37.0
5	36.4309230688122	37.0	37.0	37.0	35.0	37.0
6	36.46012533949077	37.0	37.0	37.0	35.0	37.0
7	36.435718552812745	37.0	37.0	37.0	35.0	37.0
8	36.4215683737725	37.0	37.0	37.0	35.0	37.0
9	38.21346655613642	39.0	39.0	39.0	37.0	39.0
10-11	38.156200119478754	39.0	39.0	39.0	37.0	39.0
12-13	38.12823112573635	39.0	39.0	39.0	37.0	39.0
14-15	39.63300016851227	41.0	40.0	41.0	37.0	41.0
16-17	39.606913838479855	41.0	40.0	41.0	37.0	41.0
18-19	39.61789567958475	41.0	40.0	41.0	37.0	41.0
20-21	39.613959290997215	41.0	40.0	41.0	37.0	41.0
22-23	39.5559338965934	41.0	40.0	41.0	37.0	41.0
24-25	39.49233832179267	41.0	39.5	41.0	37.0	41.0
26-27	39.44267915032501	41.0	39.0	41.0	36.5	41.0
28-29	39.27203471614666	41.0	39.0	41.0	36.0	41.0
30-31	39.18721918251144	41.0	39.0	41.0	36.0	41.0
32-33	39.083683870227226	41.0	39.0	41.0	35.0	41.0
34-35	38.9883583794658	40.0	39.0	41.0	35.0	41.0
36-37	39.08179281243518	40.5	39.0	41.0	35.0	41.0
38-39	39.10768673610083	41.0	39.0	41.0	35.0	41.0
40-41	39.10325066775985	41.0	39.0	41.0	35.0	41.0
42-43	39.06290540663962	40.5	39.0	41.0	35.0	41.0
44-45	38.997960836104596	40.0	39.0	41.0	35.0	41.0
46-47	38.91190127579495	40.0	38.5	41.0	35.0	41.0
48-49	38.83908853811273	40.0	38.0	41.0	35.0	41.0
50-51	38.75118806867978	40.0	38.0	41.0	35.0	41.0
52-53	38.64654348645777	40.0	38.0	41.0	35.0	41.0
54-55	38.52831564323687	40.0	38.0	41.0	35.0	41.0
56-57	38.36086923185507	40.0	37.5	41.0	34.0	41.0
58-59	38.201848953995366	40.0	37.0	41.0	34.0	41.0
60-61	38.013213542680724	40.0	36.5	41.0	34.0	41.0
62-63	37.76696725662803	39.0	36.0	41.0	34.0	41.0
64-65	37.47508697536969	39.0	35.5	40.5	33.0	41.0
66-67	37.152526009293624	38.5	35.0	40.0	33.0	41.0
68-69	36.84793348315066	37.5	35.0	40.0	33.0	41.0
70-71	36.524799023856325	37.0	35.0	39.5	32.5	41.0
72-73	36.18555655880682	36.5	35.0	39.0	32.0	41.0
74-75	35.81747659172035	36.0	35.0	39.0	32.0	40.0
76-77	34.871230881152144	35.0	34.0	37.0	30.5	39.0
78-79	35.10667673062987	35.0	34.0	37.0	31.0	39.0
80-81	34.924655464199766	35.0	34.0	37.0	31.0	39.0
82-83	34.65065886177203	35.0	34.0	36.0	31.0	38.5
84-85	34.39915914584529	35.0	34.0	36.0	31.0	37.0
86-87	34.14315933869773	35.0	34.0	35.5	31.0	37.0
88-89	33.84328985824786	35.0	34.0	35.0	31.0	36.5
90-91	33.75839316878533	35.0	34.0	35.0	31.0	36.0
92-93	33.67099669242161	35.0	34.0	35.0	31.0	36.0
94-95	33.56149282845004	35.0	33.5	35.0	30.5	35.5
96-97	33.52345200423745	35.0	33.0	35.0	30.0	35.0
98-99	33.52464761912938	34.5	33.0	35.0	30.5	35.0
100-101	32.4697681808606	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	9.0
20	23.0
21	125.0
22	517.0
23	1893.0
24	4880.0
25	9593.0
26	17440.0
27	27358.0
28	39525.0
29	52368.0
30	67868.0
31	85560.0
32	108634.0
33	143207.0
34	209060.0
35	318573.0
36	594662.0
37	1413187.0
38	2061325.0
39	977287.0
40	50428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.28413000675181	21.184809635280846	15.469364157175722	24.06169620079162
2	13.735003943745069	21.554826951506893	41.4333883910442	23.27678071370384
3	16.81429281841668	26.615061797275825	31.401182982198666	25.169462402108827
4	11.918848876652072	18.694967676204108	39.21172793835231	30.17445550879151
5	12.968153278267655	37.96818157927719	35.703858236200226	13.359806906254928
6	29.409018318839173	38.41211930088421	18.85007338047473	13.328788999801894
7	26.019883480700734	33.09361569654849	23.28471219894801	17.601788623802765
8	24.502880718864933	36.45366737359882	21.248410696421427	17.795041211114825
9	25.430726377324973	16.87500040430125	21.55064730246973	36.143625915904046
10-11	23.811138711534603	27.05951988200819	29.054141033851486	20.075200372605718
12-13	24.76166510822466	24.700437905492755	29.114100919119608	21.423796067162975
14-15	21.977718530727785	26.05118052424475	26.920434043758867	25.0506669012686
16-17	21.38971364917607	29.213691749415155	27.57863272529325	21.81796187611553
18-19	21.430167836279818	28.22982389012353	29.4619283560868	20.878079917509847
20-21	22.28494494320468	27.33191959908707	29.38513072324838	20.998004734459876
22-23	22.016211563040677	27.249254298021206	29.203399843035648	21.531134295902472
24-25	21.73803150769203	27.438720889096086	29.31125432086703	21.51199328234485
26-27	21.652430457402776	27.609268279501425	29.21701685060823	21.521284412487564
28-29	21.57032756628898	27.577895791633626	29.294680352577384	21.55709628950001
30-31	21.888480845083908	27.470494087031543	29.17408803618472	21.466937031699832
32-33	21.711673190413595	27.632604726092218	29.08357237449728	21.572149708996907
34-35	21.79745366698169	27.652517227432117	29.10977850636673	21.440250599219464
36-37	21.811425048915645	27.543709603136946	29.03726536623531	21.6075999817121
38-39	21.65801740008121	27.688539878729596	29.082533471168897	21.570909250020296
40-41	21.83258994871184	27.593154739004568	28.963919577118812	21.61033573516478
42-43	21.812198630910785	27.641925282960766	28.73407736443827	21.811798721690177
44-45	21.84834882746291	27.77064626416021	28.543051516737787	21.837953391639093
46-47	22.001550375882527	27.740105996402605	28.20110978116371	22.05723384655116
48-49	21.934081831425228	27.82952012147964	28.215701795397607	22.020696251697526
50-51	21.961369132912747	28.01531056632237	28.017394048244277	22.005926252520602
52-53	22.09423005592045	27.941903852791082	27.888373749359946	22.075492341928523
54-55	22.069711249415242	27.86343525339292	27.92633037757261	22.140523119619225
56-57	22.05503590806629	28.009353654806812	27.848034613903334	22.08757582322357
58-59	22.007540191146703	27.9814547713487	27.970735347145116	22.04026969035948
60-61	22.116500387806447	28.018027710492472	27.85430721743135	22.011164684269733
62-63	22.066623606597688	28.101798289093242	27.812100823762187	22.019477280546884
64-65	22.052180223302294	28.001169150730192	27.816020022164384	22.13063060380313
66-67	22.129870716322664	28.058464789412717	27.638358074904474	22.173306419360145
68-69	22.069071849650022	28.029584990509253	27.73622780337972	22.16511535646101
70-71	22.06116586957818	27.805616537971385	27.807846200169056	22.325371392281383
72-73	22.107310481200336	27.836497626541508	27.69408057352377	22.362111318734385
74-75	22.0358059155571	27.956867553086983	27.663102566233288	22.344223965122627
76-77	22.181128847957872	27.833911807596508	27.653979198305784	22.330980146139844
78-79	22.142517035777225	27.796509185951066	27.609121791466034	22.451851986805675
80-81	22.169958803836074	27.85796581850918	27.535898852605307	22.436176525049436
82-83	22.20489979074709	27.82258682061319	27.62781274282527	22.34470064581446
84-85	22.238222743079884	27.7849559889431	27.634989745614817	22.341831522362202
86-87	22.19301962156235	27.782454456869445	27.692261360101583	22.332264561466623
88-89	22.266190334615672	27.702261267948895	27.675821203740597	22.355727193694833
90-91	22.264383953602493	27.759972026627228	27.680730374427657	22.294913645342618
92-93	22.322414558175865	27.667174158528713	27.71211780974105	22.298293473554374
94-95	22.217494142454715	27.646446269774195	27.76381539452589	22.3722441932452
96-97	22.220462773898355	27.680462765014745	27.79111923270483	22.307955228382067
98-99	22.422792079799066	28.27245063360063	27.862682922134454	21.442074364465853
100-101	22.929651459286525	30.932358426479308	25.07237502862717	21.065615085607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1789.0
1	1481.0
2	1694.0
3	2340.0
4	3171.0
5	4860.5
6	6271.0
7	7750.5
8	9065.0
9	9216.0
10	8436.5
11	7747.5
12	8760.5
13	12031.5
14	19325.0
15	29164.0
16	36872.0
17	40679.5
18	40792.5
19	38234.0
20	34402.0
21	30627.5
22	28736.0
23	30190.0
24	35150.0
25	43571.5
26	54702.5
27	67191.0
28	80886.0
29	95159.0
30	108893.0
31	122949.0
32	138299.5
33	153698.0
34	167988.5
35	181716.5
36	197374.5
37	209958.0
38	218013.5
39	226460.0
40	234257.0
41	240732.5
42	244056.5
43	246484.0
44	249105.0
45	251174.5
46	252554.5
47	251933.0
48	249740.0
49	246442.0
50	241839.0
51	233899.5
52	222574.0
53	209151.5
54	195129.0
55	181331.0
56	166727.5
57	150510.5
58	133176.0
59	115046.5
60	96687.0
61	78980.5
62	62702.5
63	48508.5
64	36167.5
65	26769.0
66	20214.0
67	15477.5
68	11891.5
69	8687.0
70	6299.0
71	4609.0
72	3212.0
73	2213.5
74	1487.0
75	932.5
76	575.5
77	359.0
78	235.5
79	148.0
80	93.0
81	60.0
82	39.5
83	29.5
84	24.5
85	17.5
86	13.0
87	11.0
88	9.0
89	6.5
90	4.5
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6172002834628657E-5
2	0.005126524898577284
3	1.6172002834628657E-5
4	1.6172002834628657E-5
5	1.6172002834628657E-5
6	1.6172002834628657E-5
7	1.6172002834628657E-5
8	0.0
9	2.9109605102331585E-4
10-11	7.439121303929181E-4
12-13	0.0
14-15	3.2344005669257314E-5
16-17	0.0012856742253529781
18-19	0.0
20-21	0.0
22-23	7.439780034816554E-4
24-25	0.002499161486837709
26-27	0.007022009469357747
28-29	0.36234341250077895
30-31	0.3327783786568822
32-33	0.33145407617404327
34-35	0.1855279866472604
36-37	0.04895913804449567
38-39	0.04749993567203243
40-41	0.026642225189086596
42-43	0.02604563391632963
44-45	0.014334738322544888
46-47	0.009482015409590807
48-49	0.008974382381204505
50-51	0.008039346115893657
52-53	0.007081674881438564
54-55	8.864570514630447E-4
56-57	0.003566254594650034
58-59	0.0029096008438199454
60-61	0.005779625057177326
62-63	0.001480111209926443
64-65	7.330093248865004E-5
66-67	6.569907657634524E-4
68-69	0.0014492336265584872
70-71	0.003948991500315399
72-73	0.002156775433678869
74-75	0.0013592458942338957
76-77	4.286178807300609E-4
78-79	1.8711130089230424E-4
80-81	0.0011155779605222858
82-83	0.0013199041648820251
84-85	0.0
86-87	0.004486247794132586
88-89	0.002037023901811687
90-91	0.005072140342628905
92-93	0.013160106935268924
94-95	0.0015165995089490827
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	332.0
22-23	703.0
24-25	1416.0
26-27	2122.0
28-29	4251.0
30-31	11553.0
32-33	16067.0
34-35	23213.0
36-37	32172.0
38-39	37002.0
40-41	40317.0
42-43	49137.0
44-45	60029.0
46-47	58599.0
48-49	51016.0
50-51	45101.0
52-53	42384.0
54-55	45827.0
56-57	48075.0
58-59	48212.0
60-61	47897.0
62-63	48392.0
64-65	52329.0
66-67	55748.0
68-69	55406.0
70-71	53900.0
72-73	52160.0
74-75	53962.0
76-77	54671.0
78-79	57185.0
80-81	57922.0
82-83	55845.0
84-85	57384.0
86-87	61021.0
88-89	63690.0
90-91	67531.0
92-93	69282.0
94-95	75977.0
96-97	114155.0
98-99	513772.0
100-101	3897692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.40866056619734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.66668398293758	81.04128749476406
2	6.108201120369946	10.80035759041706
3	1.3243021994074529	3.5123935090344625
4	0.44646084161213606	1.578840200087445
5	0.18591812408405875	0.8218386162625855
6	0.08885906925159226	0.4713546775015346
7	0.056910972676159396	0.3521996006073095
8	0.0336232825134276	0.23780714966807837
9	0.022428056840701628	0.17845510179500737
>10	0.06519693539779382	0.8680957082000262
>50	9.369860934328656E-4	0.053898198682427824
>100	4.5275554093365355E-4	0.07191917718720331
>500	2.567327484526668E-5	0.01155297579281698
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6172002834628657E-5	0.0	0.0	0.0
3	0.0	1.6172002834628657E-5	0.0	0.0	0.0
4	0.0	3.2344005669257314E-5	0.0	1.6172002834628657E-5	0.0
5	0.0	3.2344005669257314E-5	0.0	3.2344005669257314E-5	0.0
6	0.0	3.2344005669257314E-5	0.0	3.2344005669257314E-5	0.0
7	0.0	3.2344005669257314E-5	0.0	4.851600850388597E-5	0.0
8	0.0	3.2344005669257314E-5	0.0	4.851600850388597E-5	1.6172002834628657E-5
9	0.0	2.1023603685017253E-4	0.0	4.851600850388597E-5	1.6172002834628657E-5
10-11	1.6172002834628657E-5	2.1023603685017253E-4	0.0	4.851600850388597E-5	1.6172002834628657E-5
12-13	1.6172002834628657E-5	2.1023603685017253E-4	0.0	4.851600850388597E-5	1.6172002834628657E-5
14-15	1.6172002834628657E-5	2.2640803968480117E-4	0.0	7.277401275582895E-5	3.2344005669257314E-5
16-17	3.2344005669257314E-5	2.4258004251942984E-4	0.0	8.086001417314329E-5	4.851600850388597E-5
18-19	3.2344005669257314E-5	2.7492404818868716E-4	0.0	8.894601559045762E-5	6.468801133851463E-5
20-21	3.2344005669257314E-5	3.153540552752588E-4	0.0	1.6172002834628657E-4	6.468801133851463E-5
22-23	3.2344005669257314E-5	3.2344005669257314E-4	0.0	4.285580751176594E-4	8.086001417314329E-5
24-25	4.851600850388597E-5	3.396120595272018E-4	0.0	0.001503996263620465	9.703201700777194E-5
26-27	4.851600850388597E-5	3.5578406236183043E-4	0.0	0.0047303108291288825	9.703201700777194E-5
28-29	4.851600850388597E-5	3.5578406236183043E-4	0.0	0.020481841590057195	9.703201700777194E-5
30-31	5.66020099212003E-5	3.8004206661377345E-4	0.0	0.02835760697052135	9.703201700777194E-5
32-33	6.468801133851463E-5	3.8812806803108777E-4	0.0	0.03164052354595097	9.703201700777194E-5
34-35	1.1320401984240059E-4	4.447300779522881E-4	0.0	0.035950362301379504	9.703201700777194E-5
36-37	1.455480255116579E-4	5.17504090708117E-4	0.0	0.04228978741255394	1.0511801842508626E-4
38-39	1.455480255116579E-4	5.4176209496006E-4	0.0	0.0534403833670304	1.132040198424006E-4
40-41	1.455480255116579E-4	5.983641048812602E-4	0.0	0.07824014971393345	1.132040198424006E-4
42-43	1.455480255116579E-4	6.307081105505176E-4	0.0	0.08966566971659859	1.132040198424006E-4
44-45	1.455480255116579E-4	6.953961218890323E-4	0.0	0.10577298453988873	1.132040198424006E-4
46-47	1.5363402692897222E-4	7.439121303929183E-4	0.0	0.12401500373734985	1.132040198424006E-4
48-49	1.6172002834628657E-4	7.600841332275469E-4	0.0	0.13992016852520714	1.132040198424006E-4
50-51	1.7789203118091521E-4	8.247721445660615E-4	0.0	0.15487927114723865	1.132040198424006E-4
52-53	1.7789203118091521E-4	8.490301488180045E-4	0.0	0.17535302673587855	1.3746202409434358E-4
54-55	1.7789203118091521E-4	8.571161502353189E-4	0.0	0.22230035096480552	1.6172002834628657E-4
56-57	1.7789203118091521E-4	8.732881530699475E-4	0.0	0.2707597574587703	1.6172002834628657E-4
58-59	1.7789203118091521E-4	8.732881530699475E-4	0.0	0.3077855579486526	1.6172002834628657E-4
60-61	1.7789203118091521E-4	8.975461573218905E-4	0.0	0.3338305685138221	1.698060297636009E-4
62-63	1.9406403401554389E-4	9.056321587392048E-4	0.0	0.3508354294944341	1.7789203118091521E-4
64-65	1.9406403401554389E-4	9.864921729123481E-4	0.0	0.3643713958670183	1.7789203118091521E-4
66-67	1.9406403401554389E-4	0.0010026641757469768	0.0	0.3729991593792927	1.7789203118091521E-4
68-69	1.9406403401554389E-4	0.0010188361785816054	0.0	0.3817482129128268	1.7789203118091521E-4
70-71	2.021500354328582E-4	0.001035008181416234	0.0	0.38940565625502344	1.8597803259822956E-4
72-73	2.1023603685017253E-4	0.0010430941828335483	0.0	0.39427342910824664	2.1023603685017253E-4
74-75	2.1023603685017253E-4	0.0010511801842508627	0.0	0.3983649458254077	2.1023603685017253E-4
76-77	2.1023603685017253E-4	0.00108352418992012	0.0	0.40200364646319914	2.1023603685017253E-4
78-79	2.1023603685017253E-4	0.0011158681955893773	0.0	0.405060154998944	2.1023603685017253E-4
80-81	2.1023603685017253E-4	0.0011239541970066917	0.0	0.4063700872285489	2.1023603685017253E-4
82-83	2.1023603685017253E-4	0.001132040198424006	0.0	0.4067905593022492	2.4258004251942984E-4
84-85	2.1023603685017253E-4	0.0011482122012586346	0.0	0.40699270933768206	2.4258004251942984E-4
86-87	2.1023603685017253E-4	0.0011643842040932632	0.0	0.4070250533433514	2.4258004251942984E-4
88-89	2.1023603685017253E-4	0.0012048142111798351	0.0	0.4070654833504379	2.4258004251942984E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8305	0.0	53.022694	1
GTATCAA	15630	0.0	38.358818	1
ATCAACG	19675	0.0	29.949127	3
TCAACGC	19670	0.0	29.869963	4
TATCAAC	20245	0.0	29.424393	2
CAACGCA	20040	0.0	29.31847	5
AACGCAG	20645	0.0	28.583662	6
ACGCAGA	23740	0.0	24.78509	7
CGCAGAG	24320	0.0	24.176403	8
GTGGTAT	3805	0.0	22.493727	1
TGGTATC	3635	0.0	21.544315	2
GCAGAGT	27800	0.0	21.02703	9
GAGTACT	15700	0.0	20.088634	12-13
GTACATG	19985	0.0	20.085596	1
AGAGTAC	25305	0.0	19.371626	10-11
TACATGG	20350	0.0	19.346815	2
ACATGGG	20305	0.0	18.94618	3
CAGAGTA	27205	0.0	18.608582	10-11
GTACTTT	17515	0.0	18.031664	14-15
AGTACTT	17215	0.0	17.798712	12-13
>>END_MODULE