FastQCFastQC Report
Fri 27 May 2016
SRR2139625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7759487
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101900.052.652131
GTATCAA193450.038.6247521
TCAACGC242750.030.2144554
ATCAACG245100.029.924763
TATCAAC250900.029.473432
CAACGCA248300.029.4708845
AACGCAG253250.028.9455726
ACGCAGA292450.025.0510677
CGCAGAG300900.024.319118
GCAGAGT341700.021.2399029
GTGGTAT49150.021.1700441
GTACATG233050.020.008651
GAGTACT203150.019.91786612-13
TACATGG235550.019.3236522
TGGTATC49200.019.2338492
AGAGTAC312700.019.1431210-11
ACATGGG234750.018.9507583
CAGAGTA332850.018.69820410-11
AGTACTT219200.017.88321712-13
GTACTTT226100.017.82083714-15
Produced by FastQC (version 0.11.5)