FastQCFastQC Report
Fri 27 May 2016
SRR2139624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7058428
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94850.052.0207941
GTATCAA178050.037.798211
TCAACGC220400.029.7383964
ATCAACG221350.029.5914463
CAACGCA225650.029.0086025
TATCAAC227900.029.0050542
AACGCAG232550.028.1478886
ACGCAGA263050.024.8191957
CGCAGAG268400.024.3563358
GCAGAGT308950.021.1457029
GTACATG212350.019.9741331
GAGTACT187500.019.90743412-13
TACATGG209800.019.4424672
AGAGTAC280450.019.39216610-11
CTTATAC71100.019.0633031
ACATGGG211350.018.8134163
CAGAGTA301750.018.7317710-11
GTGGTAT46800.018.7290821
TGGTATC45000.017.9579932
GTACTTT210600.017.76496514-15
Produced by FastQC (version 0.11.5)