##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7058428
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93373439525062	34.0	31.0	34.0	31.0	34.0
2	33.06176573594007	34.0	33.0	34.0	31.0	34.0
3	33.121220475720655	34.0	33.0	34.0	31.0	34.0
4	36.477291260886986	37.0	37.0	37.0	35.0	37.0
5	36.44372160486726	37.0	37.0	37.0	35.0	37.0
6	36.47175787583298	37.0	37.0	37.0	35.0	37.0
7	36.44718894915412	37.0	37.0	37.0	35.0	37.0
8	36.433611280018724	37.0	37.0	37.0	35.0	37.0
9	38.229472341433535	39.0	39.0	39.0	37.0	39.0
10-11	38.171389861878595	39.0	39.0	39.0	37.0	39.0
12-13	38.142809489591734	39.0	39.0	39.0	37.0	39.0
14-15	39.65437736277823	41.0	40.0	41.0	37.0	41.0
16-17	39.627719373208876	41.0	40.0	41.0	37.0	41.0
18-19	39.640075878085035	41.0	40.0	41.0	37.0	41.0
20-21	39.636961987942044	41.0	40.0	41.0	37.0	41.0
22-23	39.58068231846116	41.0	40.0	41.0	37.0	41.0
24-25	39.515922934492785	41.0	39.5	41.0	37.0	41.0
26-27	39.46656264947171	41.0	39.0	41.0	36.5	41.0
28-29	39.295797131708426	41.0	39.0	41.0	36.0	41.0
30-31	39.211366945300135	41.0	39.0	41.0	36.0	41.0
32-33	39.102631123360695	41.0	39.0	41.0	35.0	41.0
34-35	39.00206826751022	40.5	39.0	41.0	35.0	41.0
36-37	39.09555942659699	40.5	39.0	41.0	35.0	41.0
38-39	39.117720456906056	41.0	39.0	41.0	35.0	41.0
40-41	39.11746588245728	41.0	39.0	41.0	35.0	41.0
42-43	39.08065302058416	41.0	39.0	41.0	35.0	41.0
44-45	39.01751353909553	40.0	39.0	41.0	35.0	41.0
46-47	38.934390671315995	40.0	39.0	41.0	35.0	41.0
48-49	38.86417179042123	40.0	38.0	41.0	35.0	41.0
50-51	38.77242803977499	40.0	38.0	41.0	35.0	41.0
52-53	38.67161478406847	40.0	38.0	41.0	35.0	41.0
54-55	38.55491380233853	40.0	38.0	41.0	35.0	41.0
56-57	38.382245453709224	40.0	37.5	41.0	34.0	41.0
58-59	38.22763431232059	40.0	37.0	41.0	34.0	41.0
60-61	38.041887350452384	40.0	37.0	41.0	34.0	41.0
62-63	37.798702688482045	39.0	36.0	41.0	34.0	41.0
64-65	37.513172707633444	39.0	35.5	40.5	33.5	41.0
66-67	37.20002177316283	38.5	35.0	40.0	33.0	41.0
68-69	36.89958126425269	38.0	35.0	40.0	33.0	41.0
70-71	36.57888269445739	37.0	35.0	40.0	32.5	41.0
72-73	36.240844123115394	37.0	35.0	39.0	32.0	41.0
74-75	35.87403900801182	36.0	35.0	39.0	32.0	40.0
76-77	34.92593507663353	35.0	34.0	37.0	30.5	39.0
78-79	35.1561721231715	35.0	34.0	37.0	31.5	39.0
80-81	34.97165693352763	35.0	34.0	37.0	31.5	39.0
82-83	34.69273380948367	35.0	34.0	36.5	31.0	38.5
84-85	34.43542738837343	35.0	34.0	36.0	31.0	37.0
86-87	34.17684101905242	35.0	34.0	36.0	31.0	37.0
88-89	33.86679679730155	35.0	34.0	35.0	31.0	36.5
90-91	33.78394009257912	35.0	34.0	35.0	31.0	36.0
92-93	33.69364245255794	35.0	34.0	35.0	31.0	36.0
94-95	33.58194733505847	35.0	34.0	35.0	30.5	36.0
96-97	33.543448597811164	35.0	33.0	35.0	31.0	35.0
98-99	33.5422626001448	35.0	33.0	35.0	31.0	35.0
100-101	32.49102627263825	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	96.0
22	557.0
23	2036.0
24	5159.0
25	10950.0
26	19306.0
27	30160.0
28	44156.0
29	59228.0
30	76610.0
31	96460.0
32	122496.0
33	162590.0
34	235718.0
35	357558.0
36	668922.0
37	1592642.0
38	2383688.0
39	1131882.0
40	58191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.31093995871885	21.119960580452275	15.456659111158903	24.112440349669974
2	13.649243237307887	21.472564917239595	41.69801042076218	23.180181424690335
3	16.72969062370412	26.599269213948094	31.538443338721216	25.132596823626567
4	11.780940963325252	18.88000242546993	39.27739697207281	30.06165963913201
5	12.856207197439314	37.96290589957224	35.929450003520614	13.251436899467828
6	29.241302630175248	38.574713601203214	18.878753013950558	13.305230754670976
7	25.861186918841835	33.1907094881055	23.224636310611416	17.72346728244126
8	24.33618363748982	36.539311586092545	21.312408371949108	17.81209640446853
9	25.252188381129244	16.901573072931114	21.440000544031562	36.40623800190808
10-11	23.700440597287788	27.090053189311476	29.22845675625247	19.98104945714827
12-13	24.673921728747533	24.674693855345694	29.197542285619406	21.453842130287367
14-15	21.864293824147197	26.068687268237987	26.997775424719332	25.069243482895477
16-17	21.323837975179952	29.259997342146	27.607431280448086	21.808733402225965
18-19	21.337197367062092	28.238267534678762	29.507502167267578	20.917032930991567
20-21	22.18178263355088	27.362928425044963	29.421040439824804	21.034248501579352
22-23	21.857478961115877	27.403632736425763	29.27370775309181	21.46518054936655
24-25	21.645918685979094	27.591844294525554	29.341099835112523	21.42113718438283
26-27	21.612179614168006	27.750317937906793	29.24985507361424	21.38764737431096
28-29	21.490874560023112	27.708028615353196	29.28495416911123	21.516142655512464
30-31	21.859690598686203	27.628319655350435	29.206081213394334	21.305908532569028
32-33	21.6918111659614	27.707861653977407	29.167386107005346	21.432941073055847
34-35	21.733005057251233	27.788581305311116	29.182692441659704	21.295721195777947
36-37	21.74208345142804	27.661257666364513	29.072547931167	21.52411095104044
38-39	21.5706557348115	27.75382001989029	29.166153080311457	21.509371164986753
40-41	21.79275560140561	27.658654920601883	29.041647088764783	21.506942389227724
42-43	21.793362653117455	27.666691086847894	28.843413030686538	21.696533229348116
44-45	21.789571534663978	27.879489841930305	28.604945868251434	21.725992755154287
46-47	21.957710558401132	27.761212401685846	28.238198603493576	22.042878436419443
48-49	21.87283951664155	27.880436238571317	28.2347132986758	22.012010946111328
50-51	21.846213564611006	28.030449777767014	28.15641206852978	21.966924589092194
52-53	22.000288462388742	28.008950872049855	28.03857467867394	21.952185986887464
54-55	21.982203239119823	27.959546170618786	27.978896485337835	22.07935410492355
56-57	22.054227968213873	28.068803474513842	27.834561454410782	22.0424071028615
58-59	21.887032424793684	28.1965006519312	28.043300723760744	21.873166199514372
60-61	22.106324818773015	28.232302924639423	27.797460557754178	21.863911698833384
62-63	21.978975958744556	28.37758511946258	27.746213942049845	21.897224979743015
64-65	21.98496856823688	28.267423798323478	27.749631712279278	21.997975921160364
66-67	22.053351655207393	28.234342818900267	27.615550150458667	22.09675537543367
68-69	22.026719086451696	28.13892182142264	27.758156575989084	22.07620251613658
70-71	22.041993877444614	27.852446249707235	27.835819845587135	22.26974002726101
72-73	22.071538102724055	27.91186713289694	27.737385994052293	22.279208770326704
74-75	21.977795655237163	28.0199717725214	27.689669250963995	22.31256332127744
76-77	22.129681074538045	27.93771459774572	27.66729628413142	22.26530804358481
78-79	22.104395865764186	27.936787584055946	27.62203882464382	22.33677772553605
80-81	22.111424086629174	27.913801976364006	27.6018004525181	22.372973484488718
82-83	22.161462514577718	27.876421487234794	27.68300070198641	22.279115296201077
84-85	22.30592507483801	27.833057898077012	27.642988858372103	22.218028168712873
86-87	22.1459102486082	27.828205648342237	27.724777424662474	22.30110667838709
88-89	22.246856848660446	27.813208310169905	27.653579217089906	22.28635562407974
90-91	22.213065731076384	27.886128733090803	27.6729288616805	22.227876674152313
92-93	22.263359864625844	27.78785025960727	27.772582003765734	22.176207872001154
94-95	22.16954951834342	27.77160452545123	27.792858423507592	22.265987532697757
96-97	22.171888203229635	27.800633086228483	27.8334330708753	22.19404563966658
98-99	22.421730285236798	28.31139940403385	27.8854707829843	21.381399527745053
100-101	22.890341707100877	31.065670941022343	25.110385501519854	20.933601850356926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1820.0
1	1569.5
2	1873.5
3	2570.5
4	3582.0
5	5683.0
6	7484.0
7	9064.5
8	10244.0
9	10318.0
10	9527.5
11	8901.0
12	10015.5
13	13654.5
14	22223.5
15	33827.5
16	43062.0
17	47915.0
18	48365.0
19	45256.5
20	40501.5
21	35962.0
22	33772.0
23	35720.0
24	41469.5
25	50919.5
26	63520.5
27	78163.0
28	94091.0
29	111273.5
30	127557.5
31	142477.0
32	158513.5
33	174993.0
34	191562.0
35	207297.0
36	224503.0
37	239264.0
38	249142.5
39	258898.0
40	268647.5
41	276926.0
42	280265.0
43	283121.0
44	286206.0
45	288124.0
46	291043.0
47	291699.5
48	290251.5
49	284751.5
50	276278.5
51	265298.5
52	250754.5
53	235933.0
54	219869.5
55	202577.0
56	184635.5
57	165762.5
58	146925.0
59	127014.0
60	105957.0
61	85890.0
62	67681.0
63	51925.0
64	38574.0
65	28827.5
66	22060.5
67	16930.0
68	13100.5
69	9710.5
70	7045.0
71	5127.5
72	3575.5
73	2434.0
74	1646.5
75	1088.0
76	686.5
77	423.0
78	268.5
79	172.0
80	113.0
81	72.0
82	49.5
83	33.5
84	22.0
85	15.5
86	12.0
87	8.5
88	7.0
89	6.5
90	5.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4167460516704286E-5
2	0.0057094865882318276
3	1.4167460516704286E-5
4	2.833492103340857E-5
5	1.4167460516704286E-5
6	1.4167460516704286E-5
7	1.4167460516704286E-5
8	0.0
9	1.9834444723386E-4
10-11	7.792103284187358E-4
12-13	0.0
14-15	2.833492103340857E-5
16-17	0.0014450809727038372
18-19	1.4167460516704286E-5
20-21	7.083893344618448E-6
22-23	8.359517040946332E-4
24-25	0.0026074087677945904
26-27	0.007689597116138856
28-29	0.3617262066060072
30-31	0.3323190495663827
32-33	0.3309968113511603
34-35	0.18549484511893602
36-37	0.049575187972886105
38-39	0.04776142014942923
40-41	0.027659871958989782
42-43	0.02691089808210428
44-45	0.014775706178312897
46-47	0.009572850919123754
48-49	0.009499314826276965
50-51	0.008542036314019782
52-53	0.007561655697692323
54-55	6.757207098876062E-4
56-57	0.0035918038751228875
58-59	0.0029277490099328765
60-61	0.006292877612430253
62-63	0.0015016719806521086
64-65	7.21739160420081E-5
66-67	7.291966278707054E-4
68-69	0.0017116616655843888
70-71	0.0041626347177141955
72-73	0.0022816758031206303
74-75	0.0016290337365290305
76-77	5.968710654472534E-4
78-79	1.8958656169086396E-4
80-81	0.001167617606976428
82-83	0.001321824879705124
84-85	8.910877766905518E-6
86-87	0.0037774397414969065
88-89	0.0019260644997923775
90-91	0.004976821241369077
92-93	0.013839926564880497
94-95	0.0013337095664510312
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	106.0
20-21	337.0
22-23	791.0
24-25	1667.0
26-27	2640.0
28-29	4847.0
30-31	13076.0
32-33	17919.0
34-35	26332.0
36-37	36123.0
38-39	42040.0
40-41	45406.0
42-43	55964.0
44-45	66842.0
46-47	65859.0
48-49	57138.0
50-51	50289.0
52-53	47013.0
54-55	51093.0
56-57	54785.0
58-59	55821.0
60-61	55725.0
62-63	56633.0
64-65	62078.0
66-67	66257.0
68-69	64434.0
70-71	60189.0
72-73	58438.0
74-75	59653.0
76-77	60969.0
78-79	63692.0
80-81	64439.0
82-83	62999.0
84-85	64131.0
86-87	67672.0
88-89	71283.0
90-91	76139.0
92-93	77749.0
94-95	85811.0
96-97	128931.0
98-99	587501.0
100-101	4467617.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.5517750288813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54412850021065	78.3675504013635
2	6.797108713099941	11.766036483661498
3	1.5379493318047004	3.993367337165362
4	0.526424944100752	1.822520533255988
5	0.23750145103081857	1.0278086079326139
6	0.11676013892176164	0.6063478365778343
7	0.07442251264821428	0.45089804002686207
8	0.04294927657005702	0.29738688986758216
9	0.027896239808745033	0.21730221648704004
>10	0.09281945213072691	1.2343124744426517
>50	0.0015033067813153265	0.08229871222950638
>100	4.6190565479641227E-4	0.08127542447654167
>500	4.948482728613012E-5	0.031180237325204144
>1k	2.474241024766385E-5	0.021714805187840816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.4167460516704286E-5	0.0	0.0	0.0
5	0.0	4.2502381550112855E-5	0.0	1.4167460516704286E-5	0.0
6	1.4167460516704286E-5	4.2502381550112855E-5	0.0	1.4167460516704286E-5	0.0
7	2.833492103340857E-5	4.2502381550112855E-5	0.0	1.4167460516704286E-5	0.0
8	2.833492103340857E-5	5.666984206681714E-5	0.0	1.4167460516704286E-5	0.0
9	2.833492103340857E-5	1.1333968413363429E-4	0.0	2.833492103340857E-5	1.4167460516704286E-5
10-11	2.833492103340857E-5	1.1333968413363429E-4	0.0	2.833492103340857E-5	1.4167460516704286E-5
12-13	2.833492103340857E-5	1.1333968413363429E-4	0.0	3.5418651291760715E-5	1.4167460516704286E-5
14-15	2.833492103340857E-5	1.345908749086907E-4	0.0	4.2502381550112855E-5	7.792103284187356E-5
16-17	7.083730258352143E-5	1.4167460516704286E-4	0.0	4.2502381550112855E-5	8.500476310022571E-5
18-19	7.083730258352143E-5	1.5584206568374713E-4	0.0	7.792103284187356E-5	8.500476310022571E-5
20-21	7.083730258352143E-5	1.8417698671715572E-4	0.0	1.1333968413363429E-4	8.500476310022571E-5
22-23	7.083730258352143E-5	1.9834444723386E-4	0.0	4.2502381550112855E-4	9.208849335857786E-5
24-25	7.083730258352143E-5	2.125119077505643E-4	0.0	0.0014167460516704285	9.917222361693E-5
26-27	7.083730258352143E-5	2.2667936826726857E-4	0.0	0.004349410378628215	9.917222361693E-5
28-29	7.083730258352143E-5	2.2667936826726857E-4	0.0	0.020188631236303607	1.1333968413363429E-4
30-31	7.083730258352143E-5	2.691817498173814E-4	0.0	0.028086990474366247	1.2750714465033857E-4
32-33	7.083730258352143E-5	2.691817498173814E-4	0.0	0.030792975433056764	1.4167460516704286E-4
34-35	7.792103284187356E-5	3.329353221425507E-4	0.0	0.03473152945670056	1.5584206568374715E-4
36-37	8.500476310022571E-5	3.9668889446772E-4	0.0	0.041028965656375614	1.5584206568374715E-4
38-39	8.500476310022571E-5	3.9668889446772E-4	0.0	0.051215369767885995	1.5584206568374715E-4
40-41	8.500476310022571E-5	4.8169365756794573E-4	0.0	0.07517254550163294	1.5584206568374715E-4
42-43	9.917222361693E-5	5.100285786013543E-4	0.0	0.08491975833712548	1.5584206568374715E-4
44-45	9.917222361693E-5	5.737821509265236E-4	0.0	0.0991580561564133	1.629257959420993E-4
46-47	9.917222361693E-5	6.092008022182843E-4	0.0	0.11483435121814659	1.7000952620045142E-4
48-49	9.917222361693E-5	6.092008022182843E-4	0.0	0.12933474705699344	1.9834444723386E-4
50-51	9.917222361693E-5	6.658706442851014E-4	0.0	0.1432684444751721	1.9834444723386E-4
52-53	9.917222361693E-5	6.658706442851014E-4	0.0	0.16334373602734206	2.1959563800891642E-4
54-55	9.917222361693E-5	6.658706442851014E-4	0.0	0.20644823464941486	2.5501428930067713E-4
56-57	1.0625595387528215E-4	6.800381048018057E-4	0.0	0.25190453171726057	2.7626548007573355E-4
58-59	1.2042341439198642E-4	6.871218350601579E-4	0.0	0.2862535397400101	2.833492103340857E-4
60-61	1.48758335425395E-4	7.012892955768622E-4	0.0	0.31067824167080826	2.833492103340857E-4
62-63	1.8417698671715572E-4	7.083730258352143E-4	0.0	0.32659538356132556	2.833492103340857E-4
64-65	2.1251190775056428E-4	7.9337778893544E-4	0.0	0.33928234445403427	2.833492103340857E-4
66-67	2.1251190775056428E-4	8.217127099688486E-4	0.0	0.3466423401924621	2.9751667085079E-4
68-69	2.1251190775056428E-4	8.854662822940179E-4	0.0	0.3546115367331083	3.187678616258465E-4
70-71	2.1251190775056428E-4	9.138012033274264E-4	0.0	0.3614685876231931	3.4001905240090284E-4
72-73	2.1251190775056428E-4	9.208849335857786E-4	0.0	0.3655417325217456	4.3919127601783285E-4
74-75	2.1251190775056428E-4	9.42136124360835E-4	0.0	0.3690056766180798	4.3919127601783285E-4
76-77	2.1251190775056428E-4	9.704710453942436E-4	0.0	0.37233502983950534	4.3919127601783285E-4
78-79	2.1251190775056428E-4	9.917222361693E-4	0.0	0.3752393592454297	4.4627500627618497E-4
80-81	2.1251190775056428E-4	9.917222361693E-4	0.0	0.3766631890273585	4.816936575679457E-4
82-83	2.1251190775056428E-4	9.917222361693E-4	0.0	0.37718738506647653	5.100285786013543E-4
84-85	2.1251190775056428E-4	0.0010058896966860043	0.0	0.3775274041188774	5.100285786013543E-4
86-87	2.1251190775056428E-4	0.0010200571572027085	0.0	0.37756990650042754	5.100285786013543E-4
88-89	2.1251190775056428E-4	0.00109089445978623	0.0	0.3775911576912026	5.100285786013543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9485	0.0	52.020794	1
GTATCAA	17805	0.0	37.79821	1
TCAACGC	22040	0.0	29.738396	4
ATCAACG	22135	0.0	29.591446	3
CAACGCA	22565	0.0	29.008602	5
TATCAAC	22790	0.0	29.005054	2
AACGCAG	23255	0.0	28.147888	6
ACGCAGA	26305	0.0	24.819195	7
CGCAGAG	26840	0.0	24.356335	8
GCAGAGT	30895	0.0	21.145702	9
GTACATG	21235	0.0	19.974133	1
GAGTACT	18750	0.0	19.907434	12-13
TACATGG	20980	0.0	19.442467	2
AGAGTAC	28045	0.0	19.392166	10-11
CTTATAC	7110	0.0	19.063303	1
ACATGGG	21135	0.0	18.813416	3
CAGAGTA	30175	0.0	18.73177	10-11
GTGGTAT	4680	0.0	18.729082	1
TGGTATC	4500	0.0	17.957993	2
GTACTTT	21060	0.0	17.764965	14-15
>>END_MODULE