FastQCFastQC Report
Fri 27 May 2016
SRR2139623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971169
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111850.053.8569221
GTATCAA206900.039.4067421
TCAACGC262000.030.6764224
ATCAACG263250.030.5470333
TATCAAC268750.030.1782722
CAACGCA268200.029.983815
AACGCAG274850.029.3207026
ACGCAGA317650.025.3430717
CGCAGAG326050.024.663888
GCAGAGT367800.021.666199
GTGGTAT51950.021.5252021
TGGTATC49700.020.9479792
GAGTACT216050.020.33637812-13
GTACATG254000.020.0558191
TACATGG253050.019.5046582
AGAGTAC335100.019.29965610-11
CAGAGTA360200.019.21560910-11
ACATGGG257800.018.7123623
GTACTTT240000.018.36070614-15
AGTACTT234400.017.68420812-13
Produced by FastQC (version 0.11.5)