##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7971169
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98590219326676	34.0	31.0	34.0	31.0	34.0
2	33.10833931635372	34.0	33.0	34.0	31.0	34.0
3	33.16548977947902	34.0	33.0	34.0	31.0	34.0
4	36.513675346740236	37.0	37.0	37.0	35.0	37.0
5	36.485621243258045	37.0	37.0	37.0	35.0	37.0
6	36.516012519619146	37.0	37.0	37.0	35.0	37.0
7	36.493337300965514	37.0	37.0	37.0	35.0	37.0
8	36.48134106804159	37.0	37.0	37.0	35.0	37.0
9	38.28611048141119	39.0	39.0	39.0	37.0	39.0
10-11	38.228418566963015	39.0	39.0	39.0	37.0	39.0
12-13	38.20143099462575	39.0	39.0	39.0	37.0	39.0
14-15	39.723248308999594	41.0	40.0	41.0	37.0	41.0
16-17	39.69689521072756	41.0	40.0	41.0	37.0	41.0
18-19	39.70791442258971	41.0	40.0	41.0	37.0	41.0
20-21	39.700944986936356	41.0	40.0	41.0	37.0	41.0
22-23	39.64624517216562	41.0	40.0	41.0	37.0	41.0
24-25	39.58338336156713	41.0	40.0	41.0	37.0	41.0
26-27	39.53664199251223	41.0	39.5	41.0	37.0	41.0
28-29	39.36820969130592	41.0	39.0	41.0	36.5	41.0
30-31	39.28404744015513	41.0	39.0	41.0	36.0	41.0
32-33	39.17871012614462	41.0	39.0	41.0	35.5	41.0
34-35	39.084387497129924	40.5	39.0	41.0	35.0	41.0
36-37	39.18744816233625	40.5	39.0	41.0	35.0	41.0
38-39	39.21146196571809	41.0	39.0	41.0	35.0	41.0
40-41	39.204171813612234	41.0	39.0	41.0	35.0	41.0
42-43	39.16259030637125	41.0	39.0	41.0	35.0	41.0
44-45	39.09480496268634	40.5	39.0	41.0	35.0	41.0
46-47	39.00530852825683	40.0	39.0	41.0	35.0	41.0
48-49	38.92709724425795	40.0	38.5	41.0	35.0	41.0
50-51	38.832875687553624	40.0	38.0	41.0	35.0	41.0
52-53	38.724499890999546	40.0	38.0	41.0	35.0	41.0
54-55	38.60073919653051	40.0	38.0	41.0	35.0	41.0
56-57	38.42252327148161	40.0	37.5	41.0	34.5	41.0
58-59	38.25813963111575	40.0	37.0	41.0	34.0	41.0
60-61	38.060519476963755	39.5	36.5	41.0	34.0	41.0
62-63	37.80779219661306	39.0	36.0	41.0	34.0	41.0
64-65	37.50814109157828	39.0	35.5	40.5	34.0	41.0
66-67	37.18151027335813	38.0	35.0	40.0	33.0	41.0
68-69	36.87119144109387	37.5	35.0	40.0	33.0	41.0
70-71	36.544287788443874	37.0	35.0	39.5	33.0	41.0
72-73	36.20152292233344	36.5	35.0	39.0	32.5	40.5
74-75	35.83078675828811	36.0	35.0	39.0	32.0	40.0
76-77	34.89273758464523	35.0	34.0	37.0	30.5	39.0
78-79	35.12343735698474	35.0	34.0	37.0	32.0	39.0
80-81	34.94309276844247	35.0	34.0	37.0	32.0	39.0
82-83	34.673906192567486	35.0	34.0	36.0	31.5	38.5
84-85	34.424327002908306	35.0	34.0	36.0	31.0	37.0
86-87	34.17516405239249	35.0	34.0	35.5	31.0	37.0
88-89	33.87467282337917	35.0	34.0	35.0	31.0	36.5
90-91	33.797322950788	35.0	34.0	35.0	31.0	36.0
92-93	33.70774319557552	35.0	34.0	35.0	31.0	36.0
94-95	33.59749682362937	35.0	34.0	35.0	31.0	35.5
96-97	33.55730738741035	35.0	33.0	35.0	31.0	35.0
98-99	33.551995991616636	35.0	33.0	35.0	31.0	35.0
100-101	32.50388805912383	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	17.0
21	90.0
22	392.0
23	1416.0
24	3844.0
25	8466.0
26	15596.0
27	26317.0
28	39901.0
29	56660.0
30	76929.0
31	100998.0
32	134175.0
33	181694.0
34	272278.0
35	417618.0
36	791822.0
37	1869117.0
38	2666530.0
39	1243405.0
40	63900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85355467443533	20.797496878122974	15.290297554962473	24.058650892479218
2	14.021439598332156	21.35945546004681	41.15283790200815	23.466267039612884
3	16.966087520947372	26.349394806230357	31.244789030864496	25.439728641957775
4	12.151885934880795	18.53871817498218	38.82019267946496	30.489203210672066
5	13.176679547258205	37.84456376896429	35.574293647880374	13.404463035897132
6	29.828434133726482	38.067830979808974	18.669828228392184	13.433906658072356
7	26.393102339105724	32.98236418611781	22.970604300550256	17.653929174226203
8	24.78286434524221	36.14799786580864	21.054389889362525	18.014747899586624
9	25.69229964405391	16.68490535432085	21.252451496653368	36.37034350497187
10-11	24.07410508884425	26.832201904062952	28.900740015901167	20.192952991191632
12-13	25.011488277315408	24.518135294835673	28.891609248279643	21.57876717956927
14-15	22.218120634030125	25.823696831342264	26.711747476875093	25.24643505775252
16-17	21.618486623889307	28.958834601110343	27.3861600727333	22.036518702267045
18-19	21.676729181971826	27.997045100542355	29.28086574012742	21.045359977358398
20-21	22.501813285904895	27.059808419906773	29.24164158781808	21.196736706370253
22-23	22.26594446345548	27.008974009529496	29.053364477275156	21.67171704973987
24-25	22.01955810829659	27.200309143118545	29.09638317232966	21.68374957625521
26-27	21.893682936929284	27.363603145659145	29.060436988595345	21.682276928816226
28-29	21.81152877764818	27.349245888401075	29.090542602581177	21.748682731369573
30-31	22.16113740131296	27.210598514527227	28.989146853175075	21.639117230984738
32-33	21.95588491347384	27.37903660913587	28.87401642656402	21.79106205082627
34-35	22.038575788356514	27.403702389536903	28.92377562431082	21.63394619779576
36-37	22.051091708097616	27.316861420563253	28.87669177872886	21.75535509261027
38-39	21.911399034044155	27.475510565365273	28.896990813369104	21.716099587221464
40-41	22.117665245058955	27.335540684514054	28.72534449238727	21.82144957803972
42-43	22.08100455286834	27.440509613311683	28.489450590586348	21.989035243233634
44-45	22.172894858745376	27.515673826013238	28.325307966190806	21.986123349050583
46-47	22.214472237673952	27.56606205823334	28.000585996190893	22.218879707901817
48-49	22.186798113925207	27.68428593284843	27.915317345470847	22.213598607755518
50-51	22.211641988455515	27.75613527470469	27.813249791218357	22.218972945621438
52-53	22.37027154043053	27.663613010372558	27.69299221086982	22.2731232383271
54-55	22.348773760881723	27.6038571640826	27.685007913641748	22.362361161393927
56-57	22.355297307220397	27.725100780919025	27.648869301529157	22.27073261033142
58-59	22.248607178737785	27.75016746025188	27.745507636691435	22.255717724318902
60-61	22.381465625254073	27.76343075879597	27.637022306915267	22.218081309034694
62-63	22.32678190151608	27.83518091304287	27.597677560968798	22.240359624472255
64-65	22.327813992682046	27.770026064962288	27.586840085747294	22.315319856608376
66-67	22.394285320203664	27.81923619649768	27.42084374498624	22.36563473831242
68-69	22.333645206690324	27.76295565282129	27.51312011897865	22.390279021509734
70-71	22.29798824122791	27.55811472946527	27.622280395723024	22.52161663358379
72-73	22.379310585980935	27.60089059708947	27.4832976238107	22.53650119311889
74-75	22.29228319284261	27.69260834624831	27.449891758005645	22.565216702903435
76-77	22.4584838054777	27.59527466153717	27.412808257012177	22.533433275972946
78-79	22.391284021698116	27.606879367453008	27.353518141920535	22.648318468928338
80-81	22.41340118794168	27.640487326529556	27.32376376048819	22.62234772504057
82-83	22.458796565509335	27.5766576382681	27.43519780977844	22.529347986444133
84-85	22.52217489610594	27.553577757081104	27.395700218821815	22.528547127991143
86-87	22.45400794435301	27.516290052250437	27.472431761215265	22.55727024218129
88-89	22.50395867829583	27.48127415340395	27.414850031648523	22.599917136651694
90-91	22.52082899756491	27.559896731668914	27.409140281776413	22.51013398898976
92-93	22.569376494413078	27.44739686613652	27.47824233685418	22.504984302596213
94-95	22.447750817123207	27.47257013274028	27.514375851353417	22.56530319878309
96-97	22.480317831364655	27.434071184571685	27.564928876405403	22.520682107658256
98-99	22.684950972747366	28.02623986576829	27.624192244753498	21.66461691673085
100-101	23.246520839180086	30.72482974277327	24.78445815185957	21.244191266187077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2289.0
1	1927.5
2	2258.5
3	3156.0
4	4275.5
5	6593.0
6	8547.5
7	10533.0
8	12408.0
9	12832.0
10	11769.5
11	10669.0
12	11618.0
13	15438.0
14	24919.5
15	37689.0
16	47670.0
17	52920.0
18	53179.0
19	49439.5
20	44042.0
21	38511.5
22	35131.0
23	36514.0
24	42526.0
25	52649.5
26	65806.0
27	80603.0
28	97279.5
29	115552.5
30	133259.5
31	150506.0
32	168357.5
33	187670.0
34	206278.0
35	222255.0
36	240201.5
37	255952.5
38	267358.5
39	278745.0
40	289726.0
41	299086.0
42	304314.0
43	309544.5
44	314852.0
45	319175.0
46	323133.0
47	323476.0
48	322161.0
49	319478.5
50	314231.0
51	306416.5
52	294918.5
53	279831.5
54	263387.5
55	246480.0
56	227126.5
57	205524.0
58	184164.5
59	160922.5
60	135723.0
61	111370.5
62	88802.0
63	69077.0
64	52106.0
65	39033.5
66	29760.0
67	22828.0
68	17632.5
69	13170.5
70	9438.0
71	6803.0
72	4819.0
73	3371.5
74	2299.5
75	1499.5
76	988.0
77	606.0
78	384.0
79	235.5
80	147.0
81	88.5
82	57.0
83	45.5
84	35.5
85	27.5
86	20.5
87	15.5
88	10.0
89	4.5
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.763563412091753E-5
2	0.005068265394950226
3	3.763563412091753E-5
4	3.763563412091753E-5
5	3.763563412091753E-5
6	3.763563412091753E-5
7	3.763563412091753E-5
8	0.0
9	2.2581380472550515E-4
10-11	7.088044426106133E-4
12-13	0.0
14-15	2.509042274727835E-5
16-17	0.0016308774785730928
18-19	1.2545211373639176E-5
20-21	1.2545434073587126E-5
22-23	7.715876098837989E-4
24-25	0.0028044954242178906
26-27	0.007499442876116044
28-29	0.36527747094193985
30-31	0.3355719037584807
32-33	0.33431763783542506
34-35	0.18581545156381307
36-37	0.0467611737713939
38-39	0.044938526008697806
40-41	0.024541243236168523
42-43	0.024174849246182486
44-45	0.011690171509469462
46-47	0.007617107101656543
48-49	0.007582716346782717
50-51	0.006051857726180484
52-53	0.005085121837226194
54-55	3.0571557690159846E-4
56-57	0.0027799443312724087
58-59	0.0016844115511697652
60-61	0.003988389540507595
62-63	5.544357668618655E-4
64-65	7.078830135542608E-6
66-67	7.575302256346655E-4
68-69	0.0016168454507960004
70-71	0.0042576018241199266
72-73	0.002149518599850697
74-75	0.0015535642182494586
76-77	6.758247427247842E-4
78-79	1.745536189632623E-4
80-81	9.82293092622411E-4
82-83	0.0011873544423741192
84-85	0.0
86-87	0.003945820157774056
88-89	0.0017202611066970248
90-91	0.004553113164654745
92-93	0.013116278201546813
94-95	0.00136667448941083
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	78.0
20-21	342.0
22-23	784.0
24-25	2125.0
26-27	2728.0
28-29	5226.0
30-31	14590.0
32-33	20175.0
34-35	28741.0
36-37	40535.0
38-39	46382.0
40-41	51447.0
42-43	62278.0
44-45	75444.0
46-47	73598.0
48-49	63748.0
50-51	56124.0
52-53	53055.0
54-55	56630.0
56-57	59265.0
58-59	59627.0
60-61	59415.0
62-63	59532.0
64-65	65583.0
66-67	69191.0
68-69	69351.0
70-71	66769.0
72-73	65327.0
74-75	67510.0
76-77	69339.0
78-79	72544.0
80-81	72794.0
82-83	71609.0
84-85	72427.0
86-87	77063.0
88-89	80127.0
90-91	84979.0
92-93	87549.0
94-95	95767.0
96-97	144423.0
98-99	666380.0
100-101	5080568.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.0892477385635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.31987183294142	77.75569821941397
2	6.849161707189875	11.792783580235033
3	1.5856914384064533	4.095329492336768
4	0.5945485845266859	2.0473696154372045
5	0.26030545260117643	1.1204750298340787
6	0.13748187439819806	0.7101426686777126
7	0.07600683393477908	0.4580359809504536
8	0.04754663560737188	0.32746032735505937
9	0.030341992947320217	0.23509074129512372
>10	0.09674721552695872	1.261089393787558
>50	0.0018615064615795022	0.10427323367850909
>100	3.8965074505305876E-4	0.06151862712940596
>500	4.5274713063828626E-5	0.03073308986915434
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2545211373639174E-5
2	0.0	2.5090422747278348E-5	0.0	0.0	1.2545211373639174E-5
3	0.0	2.5090422747278348E-5	0.0	0.0	1.2545211373639174E-5
4	0.0	6.272605686819587E-5	0.0	1.2545211373639174E-5	1.2545211373639174E-5
5	0.0	7.527126824183504E-5	0.0	1.2545211373639174E-5	1.2545211373639174E-5
6	0.0	8.781647961547422E-5	0.0	1.2545211373639174E-5	3.763563412091752E-5
7	0.0	1.0036169098911339E-4	0.0	1.2545211373639174E-5	3.763563412091752E-5
8	0.0	1.5054253648367008E-4	0.0	1.2545211373639174E-5	3.763563412091752E-5
9	0.0	2.88539861593701E-4	0.0	1.2545211373639174E-5	3.763563412091752E-5
10-11	0.0	2.948124672805206E-4	0.0	1.881781706045876E-5	3.763563412091752E-5
12-13	3.763563412091752E-5	3.0108507296734016E-4	0.0	2.5090422747278348E-5	3.763563412091752E-5
14-15	3.763563412091752E-5	4.0771936964327315E-4	0.0	3.763563412091752E-5	5.645345118137628E-5
16-17	5.6453451181376287E-5	4.1399197533009273E-4	0.0	5.6453451181376287E-5	1.0036169098911339E-4
18-19	6.272605686819587E-5	4.3908239807737107E-4	0.0	7.527126824183504E-5	1.1290690236275256E-4
20-21	6.899866255501546E-5	5.080810606323866E-4	0.0	1.442699307968505E-4	1.1917950804957216E-4
22-23	8.781647961547422E-5	5.958975402478607E-4	0.0	4.1399197533009273E-4	1.379973251100309E-4
24-25	1.0663429667593298E-4	6.962592312369741E-4	0.0	0.0015116979705235205	1.7563295923094844E-4
26-27	1.1290690236275256E-4	7.276222596710721E-4	0.0	0.004660546025306953	1.7563295923094844E-4
28-29	1.1290690236275256E-4	7.276222596710721E-4	0.0	0.021358222363620692	1.7563295923094844E-4
30-31	1.2545211373639174E-4	8.028935279129071E-4	0.0	0.02963178926453573	1.7563295923094844E-4
32-33	1.2545211373639174E-4	8.279839506601855E-4	0.0	0.032460734429291364	1.7563295923094844E-4
34-35	1.3172471942321133E-4	9.973443042043144E-4	0.0	0.03661319939396593	1.881781706045876E-4
36-37	1.379973251100309E-4	0.0011792498691220823	0.0	0.04203900331306487	1.881781706045876E-4
38-39	1.5054253648367008E-4	0.0011917950804957215	0.0	0.051573363957030646	1.881781706045876E-4
40-41	1.5054253648367008E-4	0.0012796115601111956	0.0	0.07313858230831638	1.881781706045876E-4
42-43	1.5054253648367008E-4	0.0013235197999189327	0.0	0.08289248415132083	1.881781706045876E-4
44-45	1.5681514217048966E-4	0.00151797057621034	0.0	0.09673612490213168	2.0072338197822678E-4
46-47	1.6308774785730925E-4	0.001655967901320371	0.0	0.11164610861970183	2.0072338197822678E-4
48-49	1.6308774785730925E-4	0.001655967901320371	0.0	0.12542702331364447	2.1326859335186595E-4
50-51	1.6308774785730925E-4	0.0019633255799745308	0.0	0.13846149793085555	2.1326859335186595E-4
52-53	1.6308774785730925E-4	0.0019821433970349895	0.0	0.15787521253156217	2.2581380472550515E-4
54-55	1.6308774785730925E-4	0.002007233819782268	0.0	0.20179599755067296	2.3835901609914432E-4
56-57	1.6308774785730925E-4	0.0020448694539031854	0.0	0.24753583821896136	2.3835901609914432E-4
58-59	1.6308774785730925E-4	0.0020574146652768245	0.0	0.28042310983495644	2.3835901609914432E-4
60-61	1.6308774785730925E-4	0.0020887776937109225	0.0	0.30482981856237146	2.3835901609914432E-4
62-63	1.6308774785730925E-4	0.0021201407221450204	0.0	0.32054269580785455	2.6344943884642265E-4
64-65	1.6308774785730925E-4	0.002502769669041015	0.0	0.3335332621852579	2.6344943884642265E-4
66-67	1.6308774785730925E-4	0.002546677908848752	0.0	0.34177546605773884	2.6344943884642265E-4
68-69	1.7563295923094844E-4	0.002621949177090587	0.0	0.34937786415016414	2.6344943884642265E-4
70-71	1.881781706045876E-4	0.002653312205524685	0.0	0.3563341838568471	2.6972204453324224E-4
72-73	1.881781706045876E-4	0.0026595848112115046	0.0	0.36073755304899446	2.759946502200618E-4
74-75	1.881781706045876E-4	0.00273485607945334	0.0	0.3644007547700971	2.759946502200618E-4
76-77	1.881781706045876E-4	0.002929306855744747	0.0	0.3677628714182324	2.822672559068814E-4
78-79	2.0699598766504637E-4	0.003010850729673402	0.0	0.3707486317251585	2.88539861593701E-4
80-81	2.1326859335186595E-4	0.0030171233353602214	0.0	0.37199688025683564	2.948124672805206E-4
82-83	2.1326859335186595E-4	0.0030484863637943193	0.0	0.37249868871178116	3.0108507296734016E-4
84-85	2.1326859335186595E-4	0.0030610315751679585	0.0	0.37285622723592987	3.0108507296734016E-4
86-87	2.1326859335186595E-4	0.003098667209288876	0.0	0.37293149850417173	3.0108507296734016E-4
88-89	2.1326859335186595E-4	0.0032303919287120873	0.0	0.372956588926919	3.0108507296734016E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11185	0.0	53.856922	1
GTATCAA	20690	0.0	39.406742	1
TCAACGC	26200	0.0	30.676422	4
ATCAACG	26325	0.0	30.547033	3
TATCAAC	26875	0.0	30.178272	2
CAACGCA	26820	0.0	29.98381	5
AACGCAG	27485	0.0	29.320702	6
ACGCAGA	31765	0.0	25.343071	7
CGCAGAG	32605	0.0	24.66388	8
GCAGAGT	36780	0.0	21.66619	9
GTGGTAT	5195	0.0	21.525202	1
TGGTATC	4970	0.0	20.947979	2
GAGTACT	21605	0.0	20.336378	12-13
GTACATG	25400	0.0	20.055819	1
TACATGG	25305	0.0	19.504658	2
AGAGTAC	33510	0.0	19.299656	10-11
CAGAGTA	36020	0.0	19.215609	10-11
ACATGGG	25780	0.0	18.712362	3
GTACTTT	24000	0.0	18.360706	14-15
AGTACTT	23440	0.0	17.684208	12-13
>>END_MODULE