FastQCFastQC Report
Fri 27 May 2016
SRR2139622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979707
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.053.9662971
GTATCAA194250.038.2385181
TCAACGC242050.029.9764884
ATCAACG245100.029.6383343
TATCAAC250300.029.246632
CAACGCA249100.029.1286475
AACGCAG256600.028.2772646
ACGCAGA296950.024.4349087
CGCAGAG304200.023.8243058
GTGGTAT45950.021.8774871
GCAGAGT342600.021.0411249
TGGTATC47500.020.0782782
GAGTACT202500.020.05823512-13
GTACATG243250.019.4447151
AGAGTAC313150.019.14440210-11
CAGAGTA334400.019.0712110-11
TACATGG243700.018.9328422
ACATGGG245550.018.4397133
GTACTTT224800.018.07819714-15
AGTACTT223450.017.08178512-13
Produced by FastQC (version 0.11.5)