##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7979707
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92990970721105	34.0	31.0	34.0	31.0	34.0
2	33.058385351742864	34.0	33.0	34.0	31.0	34.0
3	33.115304108283674	34.0	33.0	34.0	31.0	34.0
4	36.47699182438654	37.0	37.0	37.0	35.0	37.0
5	36.441546412669034	37.0	37.0	37.0	35.0	37.0
6	36.47000297128704	37.0	37.0	37.0	35.0	37.0
7	36.44414387646063	37.0	37.0	37.0	35.0	37.0
8	36.42879093179737	37.0	37.0	37.0	35.0	37.0
9	38.22346434524476	39.0	39.0	39.0	37.0	39.0
10-11	38.16163507005959	39.0	39.0	39.0	37.0	39.0
12-13	38.13433538349215	39.0	39.0	39.0	37.0	39.0
14-15	39.64509003902023	41.0	40.0	41.0	37.0	41.0
16-17	39.61843837123343	41.0	40.0	41.0	37.0	41.0
18-19	39.62551193922283	41.0	40.0	41.0	37.0	41.0
20-21	39.61664433580282	41.0	40.0	41.0	37.0	41.0
22-23	39.56180078628908	41.0	40.0	41.0	37.0	41.0
24-25	39.49746138299573	41.0	39.5	41.0	37.0	41.0
26-27	39.45061613118422	41.0	39.0	41.0	36.5	41.0
28-29	39.2819284213096	41.0	39.0	41.0	36.0	41.0
30-31	39.19686265138325	41.0	39.0	41.0	36.0	41.0
32-33	39.09439468830401	41.0	39.0	41.0	35.0	41.0
34-35	38.99833252442613	40.0	39.0	41.0	35.0	41.0
36-37	39.094244990964505	40.5	39.0	41.0	35.0	41.0
38-39	39.12057555328393	41.0	39.0	41.0	35.0	41.0
40-41	39.11232347217734	41.0	39.0	41.0	35.0	41.0
42-43	39.0695260505978	40.5	39.0	41.0	35.0	41.0
44-45	39.00220232407236	40.0	39.0	41.0	35.0	41.0
46-47	38.916725425125065	40.0	38.5	41.0	35.0	41.0
48-49	38.83958499210525	40.0	38.0	41.0	35.0	41.0
50-51	38.74839062022593	40.0	38.0	41.0	35.0	41.0
52-53	38.64192560811827	40.0	38.0	41.0	35.0	41.0
54-55	38.51868999361221	40.0	38.0	41.0	35.0	41.0
56-57	38.348058293056056	40.0	37.5	41.0	34.0	41.0
58-59	38.18612516974732	40.0	37.0	41.0	34.0	41.0
60-61	37.9918270806685	39.5	36.5	41.0	34.0	41.0
62-63	37.741768736469396	39.0	36.0	41.0	34.0	41.0
64-65	37.44566537316292	39.0	35.5	40.0	33.0	41.0
66-67	37.11833310852131	38.0	35.0	40.0	33.0	41.0
68-69	36.806489339579734	37.5	35.0	40.0	33.0	41.0
70-71	36.47839381309035	37.0	35.0	39.5	32.5	41.0
72-73	36.13695112954696	36.5	35.0	39.0	32.0	40.5
74-75	35.766887649985556	36.0	35.0	39.0	32.0	40.0
76-77	34.830589066230246	35.0	34.0	37.0	30.5	39.0
78-79	35.06240686861801	35.0	34.0	37.0	31.0	39.0
80-81	34.88329782622113	35.0	34.0	37.0	31.0	39.0
82-83	34.61576529368176	35.0	34.0	36.0	31.0	38.5
84-85	34.366150543162604	35.0	34.0	36.0	31.0	37.0
86-87	34.113158210996176	35.0	34.0	35.5	31.0	37.0
88-89	33.81328391516781	35.0	34.0	35.0	31.0	36.5
90-91	33.73527914225623	35.0	34.0	35.0	31.0	36.0
92-93	33.648424901744505	35.0	34.0	35.0	31.0	36.0
94-95	33.543124056493355	35.0	33.5	35.0	30.0	35.5
96-97	33.506436364314226	35.0	33.0	35.0	30.0	35.0
98-99	33.509254260492355	34.5	33.0	35.0	30.0	35.0
100-101	32.4524576115978	33.5	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	6.0
20	29.0
21	148.0
22	627.0
23	2121.0
24	5525.0
25	11776.0
26	21272.0
27	34152.0
28	49125.0
29	66763.0
30	87545.0
31	110306.0
32	142948.0
33	188586.0
34	273029.0
35	418229.0
36	788410.0
37	1863040.0
38	2652930.0
39	1202337.0
40	60801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.488226695097126	21.025489037502012	15.48215128253488	24.00413298486598
2	13.88570496160447	21.433772376923628	41.29111850968642	23.389404151785477
3	16.873130021723863	26.357052046410235	31.3211077351857	25.448710196680203
4	12.081499253418517	18.580148012990456	38.98735604887649	30.350996684714538
5	13.134255823460162	37.822408494749055	35.66266727120021	13.380668410590566
6	29.778603372104186	38.23491742678239	18.64997281854745	13.336506382565974
7	26.38040799999398	32.98223017238981	23.014306542120725	17.623055285495482
8	24.675342089628103	36.3559589343318	21.035634516405178	17.93306445963492
9	25.487559972605432	16.933751144312087	21.262117489600154	36.31657139348232
10-11	23.980040459206037	26.965224319259917	28.92226061505888	20.132474606475164
12-13	24.958366265829056	24.51216567224837	28.989347603865657	21.54012045805692
14-15	22.13224396989062	25.866178528381873	26.77183846121649	25.229739040511017
16-17	21.62663712294329	29.05188496319765	27.374955754280723	21.94652215957834
18-19	21.66837976236217	28.109525589037993	29.22383355978278	20.998261088817056
20-21	22.492615650853796	27.195847182023087	29.170301742123034	21.141235425000083
22-23	22.247476633733648	27.058071262851197	29.019752290505707	21.67469981290945
24-25	21.989430780373375	27.243912839976065	29.081097871139207	21.685558508511356
26-27	21.86881313893608	27.41510574154499	28.995450150360767	21.720630969158158
28-29	21.82524956022163	27.389884203654336	29.079559665217065	21.705306570906977
30-31	22.093362852286134	27.263607309499367	28.975773334147075	21.667256504067424
32-33	21.914373879494274	27.416469928404375	28.870178114148516	21.79897807795284
34-35	22.043832533821	27.438221971513755	28.875221233793987	21.64272426087126
36-37	22.014718488743547	27.35595555049085	28.8343161896345	21.795009771131106
38-39	21.89996057789959	27.515664569949205	28.866567174310315	21.717807677840888
40-41	22.146474601077898	27.39703536016012	28.682510079831808	21.773979958930177
42-43	22.08958129535591	27.509241606487578	28.4433843828212	21.957792715335316
44-45	22.14921740177089	27.589368422441407	28.265948409459902	21.9954657663278
46-47	22.25902597417084	27.5614827378002	27.984440457644716	22.195050830384243
48-49	22.19155258468593	27.678717033496174	27.964807832382004	22.164922549435893
50-51	22.205276017701554	27.7903361654939	27.831691755969583	22.172696060834962
52-53	22.406641737255043	27.716242123751435	27.674214404948533	22.202901734044993
54-55	22.362922718843045	27.637940278296607	27.70264814225821	22.29648886060214
56-57	22.35337861052671	27.766812836327837	27.64989893495801	22.22990961818744
58-59	22.28089637669183	27.792473434380906	27.722245490217862	22.2043846987094
60-61	22.369531682044418	27.778826523187206	27.672131692855274	22.179510101913106
62-63	22.335559167012693	27.860564657781218	27.60765399160709	22.196222183599005
64-65	22.340264801276483	27.782221161914546	27.612193923324117	22.26532011348485
66-67	22.384346026522433	27.805794100114422	27.48960362798637	22.320256245376775
68-69	22.334433158268194	27.792065253009383	27.587126700478535	22.286374888243884
70-71	22.32373916336008	27.55465215190402	27.653443806031465	22.46816487870443
72-73	22.39737060605537	27.618162784075196	27.52202112306923	22.462445486800203
74-75	22.241131856067632	27.70289753167975	27.51774692361053	22.538223688642088
76-77	22.43495375824595	27.622847968145685	27.477476570329955	22.464721703278403
78-79	22.358389866111043	27.672208480199238	27.4059678267111	22.563433826978617
80-81	22.378179091667857	27.647382669156627	27.395603895076132	22.578834344099384
82-83	22.447904154732207	27.595937663617605	27.474437155440572	22.481721026209613
84-85	22.50747748582138	27.56942601394552	27.431807586369533	22.491288913863567
86-87	22.380377129743888	27.556214217252084	27.521189684728036	22.542218968275993
88-89	22.434172781110355	27.522710031937475	27.49692418514812	22.546193001804053
90-91	22.43939937965733	27.539991953498195	27.55478046805326	22.465828198791215
92-93	22.483240759416084	27.463839200349955	27.568238611509116	22.48468142872485
94-95	22.35697833285233	27.469560870035494	27.61784991354375	22.555610883568427
96-97	22.343203683055616	27.49460036990783	27.66924432559258	22.49295162144397
98-99	22.60669654107269	28.070528173021597	27.724221575194786	21.598553710710927
100-101	23.124036434309588	30.823389812817613	24.832831530361073	21.21974222251172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2151.0
1	1780.0
2	2025.5
3	2800.0
4	3747.0
5	5645.0
6	7339.0
7	9245.0
8	11043.5
9	11379.0
10	10305.5
11	9367.0
12	10398.5
13	14002.5
14	22811.0
15	35068.5
16	44840.5
17	49825.5
18	50201.0
19	46978.5
20	42014.5
21	37049.5
22	34105.5
23	35460.5
24	41128.5
25	51213.0
26	64920.0
27	79875.0
28	96777.0
29	115288.5
30	132202.5
31	149001.0
32	167742.5
33	187829.5
34	207220.0
35	224188.0
36	243521.5
37	260167.5
38	271966.0
39	283571.5
40	295162.5
41	306039.5
42	312264.0
43	317321.5
44	321974.5
45	325337.0
46	327933.5
47	326709.5
48	324056.5
49	321518.0
50	315992.5
51	307080.0
52	294557.0
53	278564.0
54	261227.5
55	242955.0
56	222618.5
57	200793.0
58	178675.0
59	155500.0
60	130851.0
61	106678.5
62	84625.0
63	65084.5
64	48704.5
65	36300.5
66	27422.0
67	21023.0
68	16110.0
69	12037.0
70	8861.5
71	6468.5
72	4598.0
73	3188.0
74	2157.5
75	1408.5
76	908.0
77	572.5
78	371.5
79	235.0
80	143.5
81	80.0
82	47.0
83	29.5
84	22.0
85	19.0
86	16.0
87	12.0
88	8.0
89	3.5
90	0.5
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5063576895743165E-5
2	0.004924992860013532
3	2.5063576895743165E-5
4	2.5063576895743165E-5
5	1.2531788447871583E-5
6	1.2531788447871583E-5
7	1.2531788447871583E-5
8	0.0
9	2.756993458531748E-4
10-11	8.083003548877171E-4
12-13	0.0
14-15	1.2531788447871583E-5
16-17	0.001522612296416397
18-19	1.2531788447871583E-5
20-21	1.2532040511324799E-5
22-23	7.958303502750158E-4
24-25	0.0028892226292690534
26-27	0.007829795172809033
28-29	0.3666871106395201
30-31	0.33713586483079555
32-33	0.3360307529991897
34-35	0.18775399005607646
36-37	0.049029554805437706
38-39	0.046939350667436194
40-41	0.02675328611208686
42-43	0.026042797835579914
44-45	0.014010317598174083
46-47	0.009723276342599445
48-49	0.009467266330819256
50-51	0.008047575988035138
52-53	0.006915645589629355
54-55	9.34058767593386E-4
56-57	0.0040583983745671156
58-59	0.002743042691575236
60-61	0.005696164901063437
62-63	0.0012783686953632728
64-65	1.1269865162402631E-4
66-67	6.184998861107106E-4
68-69	0.0015863643300309679
70-71	0.0038703376021562565
72-73	0.0018876773447257344
74-75	0.0012999075883878073
76-77	6.712846575877655E-4
78-79	2.486745270926376E-4
80-81	0.0011348805538217103
82-83	0.001170333403348016
84-85	7.782966681664444E-6
86-87	0.004101378519766046
88-89	0.002063781362205661
90-91	0.005238669842801967
92-93	0.014232769039643295
94-95	0.0014870220771272661
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	95.0
20-21	357.0
22-23	842.0
24-25	1915.0
26-27	2692.0
28-29	5087.0
30-31	14106.0
32-33	19555.0
34-35	28553.0
36-37	39122.0
38-39	45206.0
40-41	49662.0
42-43	60392.0
44-45	72923.0
46-47	70968.0
48-49	61459.0
50-51	54352.0
52-53	51711.0
54-55	55688.0
56-57	57706.0
58-59	57363.0
60-61	58303.0
62-63	57825.0
64-65	63675.0
66-67	67755.0
68-69	67237.0
70-71	65493.0
72-73	63802.0
74-75	65618.0
76-77	67656.0
78-79	70284.0
80-81	71117.0
82-83	69219.0
84-85	72059.0
86-87	75577.0
88-89	79287.0
90-91	84807.0
92-93	87316.0
94-95	97320.0
96-97	147231.0
98-99	677581.0
100-101	5120791.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.39322927521775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0294456141363	79.5535721135218
2	6.497566865221112	11.356867015666529
3	1.4467893787951762	3.793187876819901
4	0.5018603024607324	1.7543676990832369
5	0.2165523087683089	0.9462602785133284
6	0.10249130036168985	0.5374227426734622
7	0.06357764449693062	0.38893789636089804
8	0.037177157371602765	0.2599225470381866
9	0.023141577807488507	0.18201754936081205
>10	0.07966360520867562	1.0574106154943557
>50	0.00139927111080954	0.08285530161387478
>100	2.9246633186803535E-4	0.050772296534669074
>500	2.1253973698610237E-5	0.01376372966771
>1k	2.1253955627542076E-5	0.022642337651218054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
3	0.0	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
4	0.0	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
5	1.2531788447871583E-5	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
6	6.265894223935792E-5	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
7	6.265894223935792E-5	3.7595365343614745E-5	0.0	1.2531788447871583E-5	0.0
8	6.265894223935792E-5	7.519073068722949E-5	0.0	1.2531788447871583E-5	0.0
9	6.265894223935792E-5	8.772251913510108E-5	0.0	3.7595365343614745E-5	0.0
10-11	8.145662491116528E-5	1.0025430758297266E-4	0.0	4.386125956755054E-5	0.0
12-13	8.772251913510108E-5	1.1905199025478003E-4	0.0	5.012715379148633E-5	0.0
14-15	9.398841335903687E-5	1.8797682671807374E-4	0.0	5.6393048015422124E-5	6.265894223935791E-6
16-17	1.3158377870265163E-4	1.8797682671807374E-4	0.0	1.0652020180690845E-4	1.2531788447871583E-5
18-19	1.378496729265874E-4	2.130404036138169E-4	0.0	1.5664735559839477E-4	1.2531788447871583E-5
20-21	1.4411556715052319E-4	2.3810398050956005E-4	0.0	2.130404036138169E-4	1.2531788447871583E-5
22-23	1.7544503827020215E-4	2.6316755740530325E-4	0.0	4.5741027834731275E-4	1.8797682671807372E-5
24-25	2.0050861516594532E-4	2.6316755740530325E-4	0.0	0.001522612296416397	2.5063576895743165E-5
26-27	2.0050861516594532E-4	2.6316755740530325E-4	0.0	0.0042984034376199525	2.5063576895743165E-5
28-29	2.0050861516594532E-4	2.6316755740530325E-4	0.0	0.020658653256316304	2.5063576895743165E-5
30-31	2.130404036138169E-4	3.634218649882759E-4	0.0	0.02834690546908552	2.5063576895743165E-5
32-33	2.3183808628562426E-4	3.634218649882759E-4	0.0	0.03029559857272955	2.5063576895743165E-5
34-35	2.5063576895743167E-4	4.135490187797622E-4	0.0	0.03312151686772459	3.7595365343614745E-5
36-37	2.756993458531748E-4	4.699420667951843E-4	0.0	0.03726327294974615	3.7595365343614745E-5
38-39	2.756993458531748E-4	5.012715379148633E-4	0.0	0.044162022490299456	3.7595365343614745E-5
40-41	2.756993458531748E-4	5.70196374378157E-4	0.0	0.0574394523508194	3.7595365343614745E-5
42-43	2.756993458531748E-4	5.889940570499644E-4	0.0	0.06446351977585142	3.7595365343614745E-5
44-45	2.8823113430104637E-4	6.641847877371938E-4	0.0	0.07420071939984764	3.7595365343614745E-5
46-47	3.0076292274891796E-4	7.268437299765518E-4	0.0	0.0847336875902837	3.7595365343614745E-5
48-49	3.1329471119678954E-4	7.331096242004875E-4	0.0	0.09500348822331448	5.012715379148633E-5
50-51	3.2582649964466113E-4	8.834910855749466E-4	0.0	0.10412663021336498	5.012715379148633E-5
52-53	3.2582649964466113E-4	9.02288768246754E-4	0.0	0.1158062570467813	5.012715379148633E-5
54-55	3.2582649964466113E-4	9.336182393664329E-4	0.0	0.13896500209844798	5.6393048015422124E-5
56-57	3.2582649964466113E-4	9.524159220382402E-4	0.0	0.16427921476314855	6.265894223935792E-5
58-59	3.2582649964466113E-4	9.524159220382402E-4	0.0	0.18793296545850618	6.265894223935792E-5
60-61	3.320923938685969E-4	9.58681816262176E-4	0.0	0.205320821929928	6.265894223935792E-5
62-63	3.383582880925327E-4	9.774794989339834E-4	0.0	0.21504548976547636	7.519073068722949E-5
64-65	3.383582880925327E-4	0.0011027973834126991	0.0	0.22508971820644544	7.519073068722949E-5
66-67	3.508900765404043E-4	0.0011215950660845068	0.0	0.2313493465361573	7.519073068722949E-5
68-69	3.508900765404043E-4	0.0011278609603084424	0.0	0.23748992287561435	7.519073068722949E-5
70-71	3.508900765404043E-4	0.001134126854532378	0.0	0.24303523926379753	8.772251913510108E-5
72-73	3.5715597076434007E-4	0.001140392748756314	0.0	0.24715819766314728	1.0025430758297266E-4
74-75	3.7595365343614747E-4	0.0011466586429802498	0.0	0.25065456664010344	1.0025430758297266E-4
76-77	3.7595365343614747E-4	0.0011591904314281214	0.0	0.25414466972283567	1.1278609603084425E-4
78-79	3.7595365343614747E-4	0.0011654563256520572	0.0	0.25688286549869566	1.2531788447871583E-4
80-81	3.7595365343614747E-4	0.0011717222198759929	0.0	0.25819870328572214	1.378496729265874E-4
82-83	3.7595365343614747E-4	0.0011779881140999288	0.0	0.25868117714096517	1.5038146137445898E-4
84-85	3.7595365343614747E-4	0.0011905199025478003	0.0	0.25906339668862527	1.5038146137445898E-4
86-87	3.7595365343614747E-4	0.0012155834794435436	0.0	0.2591949804673279	1.5038146137445898E-4
88-89	3.7595365343614747E-4	0.0012531788447871582	0.0	0.25922004404422366	1.5038146137445898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10500	0.0	53.966297	1
GTATCAA	19425	0.0	38.238518	1
TCAACGC	24205	0.0	29.976488	4
ATCAACG	24510	0.0	29.638334	3
TATCAAC	25030	0.0	29.24663	2
CAACGCA	24910	0.0	29.128647	5
AACGCAG	25660	0.0	28.277264	6
ACGCAGA	29695	0.0	24.434908	7
CGCAGAG	30420	0.0	23.824305	8
GTGGTAT	4595	0.0	21.877487	1
GCAGAGT	34260	0.0	21.041124	9
TGGTATC	4750	0.0	20.078278	2
GAGTACT	20250	0.0	20.058235	12-13
GTACATG	24325	0.0	19.444715	1
AGAGTAC	31315	0.0	19.144402	10-11
CAGAGTA	33440	0.0	19.07121	10-11
TACATGG	24370	0.0	18.932842	2
ACATGGG	24555	0.0	18.439713	3
GTACTTT	22480	0.0	18.078197	14-15
AGTACTT	22345	0.0	17.081785	12-13
>>END_MODULE