FastQCFastQC Report
Fri 27 May 2016
SRR2139621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8362389
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108800.052.4455151
GTATCAA206400.038.596131
TCAACGC257100.030.5338064
ATCAACG258400.030.3965743
CAACGCA264800.029.6786125
TATCAAC266300.029.6251962
AACGCAG273300.028.8338766
ACGCAGA313700.025.1341367
CGCAGAG322500.024.461588
GTGGTAT54750.023.7682131
TGGTATC51850.022.7034052
GCAGAGT368900.021.2687849
GTACATG252500.019.953641
GAGTACT221200.019.94110112-13
TACATGG252700.019.6498762
ACATGGG254150.019.1155533
AGAGTAC336100.018.89967210-11
CAGAGTA361300.018.62994410-11
GTACTTT243500.018.09772714-15
AGTACTT241700.017.55910512-13
Produced by FastQC (version 0.11.5)