##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8362389
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94952997283432	34.0	31.0	34.0	31.0	34.0
2	33.078211740687976	34.0	33.0	34.0	31.0	34.0
3	33.135779141582624	34.0	33.0	34.0	31.0	34.0
4	36.485793354028374	37.0	37.0	37.0	35.0	37.0
5	36.4514067690465	37.0	37.0	37.0	35.0	37.0
6	36.48056972714376	37.0	37.0	37.0	35.0	37.0
7	36.456090119701436	37.0	37.0	37.0	35.0	37.0
8	36.44307015614796	37.0	37.0	37.0	35.0	37.0
9	38.24379277261558	39.0	39.0	39.0	37.0	39.0
10-11	38.18369684787445	39.0	39.0	39.0	37.0	39.0
12-13	38.156505096809056	39.0	39.0	39.0	37.0	39.0
14-15	39.67579545749426	41.0	40.0	41.0	37.0	41.0
16-17	39.649750388316065	41.0	40.0	41.0	37.0	41.0
18-19	39.659815155692954	41.0	40.0	41.0	37.0	41.0
20-21	39.653447944108144	41.0	40.0	41.0	37.0	41.0
22-23	39.59787326948779	41.0	40.0	41.0	37.0	41.0
24-25	39.533952132444995	41.0	40.0	41.0	37.0	41.0
26-27	39.48506296138949	41.0	39.5	41.0	37.0	41.0
28-29	39.318305999228215	41.0	39.0	41.0	36.0	41.0
30-31	39.233616637475016	41.0	39.0	41.0	36.0	41.0
32-33	39.13008151457875	41.0	39.0	41.0	35.5	41.0
34-35	39.03435013215557	40.5	39.0	41.0	35.0	41.0
36-37	39.12664198589475	40.5	39.0	41.0	35.0	41.0
38-39	39.15203271387427	41.0	39.0	41.0	35.0	41.0
40-41	39.14346662396477	41.0	39.0	41.0	35.0	41.0
42-43	39.10276844501022	41.0	39.0	41.0	35.0	41.0
44-45	39.035290289631284	40.5	39.0	41.0	35.0	41.0
46-47	38.94984081119307	40.0	39.0	41.0	35.0	41.0
48-49	38.87438133020757	40.0	38.5	41.0	35.0	41.0
50-51	38.78601097160306	40.0	38.0	41.0	35.0	41.0
52-53	38.68207077436247	40.0	38.0	41.0	35.0	41.0
54-55	38.56098485575906	40.0	38.0	41.0	35.0	41.0
56-57	38.393843306222635	40.0	37.5	41.0	34.0	41.0
58-59	38.23410748314559	40.0	37.0	41.0	34.0	41.0
60-61	38.043162280478185	40.0	37.0	41.0	34.0	41.0
62-63	37.79561507994361	39.0	36.0	41.0	34.0	41.0
64-65	37.50366049202496	39.0	35.5	40.5	33.5	41.0
66-67	37.182967075006836	38.5	35.0	40.0	33.0	41.0
68-69	36.87275607486832	37.5	35.0	40.0	33.0	41.0
70-71	36.548309793834704	37.0	35.0	39.5	33.0	41.0
72-73	36.20873796717614	36.5	35.0	39.0	32.0	41.0
74-75	35.841154247337315	36.0	35.0	39.0	32.0	40.0
76-77	34.9045420965446	35.0	34.0	37.0	30.5	39.0
78-79	35.13456926605878	35.0	34.0	37.0	31.5	39.0
80-81	34.95016774674918	35.0	34.0	37.0	32.0	39.0
82-83	34.67786342409018	35.0	34.0	36.0	31.0	38.5
84-85	34.426416365864846	35.0	34.0	36.0	31.0	37.0
86-87	34.174238600372675	35.0	34.0	35.5	31.0	37.0
88-89	33.870044538572174	35.0	34.0	35.0	31.0	36.5
90-91	33.78785735976305	35.0	34.0	35.0	31.0	36.0
92-93	33.699696383080564	35.0	34.0	35.0	31.0	36.0
94-95	33.590276473694125	35.0	34.0	35.0	31.0	35.5
96-97	33.552947356034785	35.0	33.0	35.0	31.0	35.0
98-99	33.55137969014771	35.0	33.0	35.0	31.0	35.0
100-101	32.488613451997026	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	30.0
21	177.0
22	716.0
23	2447.0
24	6186.0
25	12636.0
26	22566.0
27	35441.0
28	51169.0
29	68656.0
30	88897.0
31	112210.0
32	143279.0
33	189315.0
34	276486.0
35	424059.0
36	799352.0
37	1916397.0
38	2808763.0
39	1332584.0
40	71015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.581356476002256	20.9758838054532	15.379098006562478	24.063661711982064
2	13.824690308295498	21.421523341758405	41.38149676899334	23.372289580952756
3	16.90054122093579	26.474647376485354	31.245078410009384	25.37973299256947
4	11.989792867778917	18.570233765761646	39.1369666176695	30.30300674878994
5	13.044286746287453	37.88857466448882	35.67218650077149	13.394952088452236
6	29.586808267350396	38.34381538577074	18.749582206711505	13.319794140167362
7	26.2031220982425	33.013592168458075	23.151326732109688	17.63195900118973
8	24.536720308036376	36.39135897648387	21.169285475717526	17.902635239762226
9	25.42978865072397	16.898112328496502	21.45631989798103	36.215779122798494
10-11	23.88707981349898	27.004363086841938	29.021837078937544	20.086720020721536
12-13	24.8215193050694	24.623519666449383	29.064684744993325	21.490276283487887
14-15	22.0238704542291	25.96391724469135	26.889854907473797	25.12235739360575
16-17	21.453626979066463	29.1203674693349	27.501256834861703	21.92474871673693
18-19	21.4937920252215	28.181199176455436	29.39062629112327	20.934382507199796
20-21	22.32641704753146	27.267019240525798	29.34529522933475	21.061268482607996
22-23	22.051595600755235	27.140774935606405	29.198320310105796	21.609309153532568
24-25	21.774323868341355	27.347718991995162	29.276294188366304	21.601662951297183
26-27	21.703167538081487	27.498183277031423	29.210817456836715	21.58783172805038
28-29	21.632268593506527	27.49877834726842	29.243255203616	21.625697855609054
30-31	21.906447665458987	27.399693231622145	29.120396971744135	21.573462131174736
32-33	21.71554392244389	27.536347351481805	29.05709285986055	21.691015866213757
34-35	21.835995541563605	27.547471600746803	29.059973592412504	21.556559265277087
36-37	21.84077389393659	27.461818311124357	29.011636928977037	21.685770865962024
38-39	21.724177035274803	27.654861781449757	29.0298509504663	21.59111023280914
40-41	21.962780680891328	27.530454658470195	28.875178486180296	21.63158617445818
42-43	21.877674632958062	27.63519535363617	28.620442604043433	21.866687409362335
44-45	21.952979852412348	27.729672163812257	28.438790528591568	21.878557455183827
46-47	22.084923168799673	27.677185692468814	28.156261340564946	22.081629798166567
48-49	22.003648895657786	27.82269323305214	28.10831377361534	22.06534409767474
50-51	22.012589540765042	27.93106073126416	27.958775805991408	22.097573921979382
52-53	22.20994809995815	27.85769361628971	27.80473452122301	22.12762376252913
54-55	22.186472022883642	27.765438910682022	27.865246651637925	22.182842414796408
56-57	22.138572757500093	27.9108279905161	27.818253863909565	22.13234538807424
58-59	22.08310115190322	27.941367262164675	27.864914587213867	22.110616998718232
60-61	22.217658640913804	27.92760921136996	27.80210666460586	22.052625483110383
62-63	22.14377848194142	28.016521117426844	27.747570512592862	22.092129888038876
64-65	22.15986510642468	27.89957321997587	27.734328590206697	22.20623308339275
66-67	22.23045865410502	27.961154940451394	27.577709427143237	22.230676978300348
68-69	22.127736569688953	27.937386917575775	27.70289688757067	22.2319796251646
70-71	22.158856471354895	27.69510839183055	27.76662103525338	22.379414101561178
72-73	22.207357445893187	27.779273390712866	27.63009390598748	22.383275257406464
74-75	22.089422308786503	27.89225594864122	27.61578354968648	22.4025381928858
76-77	22.20239647804184	27.78099247273978	27.624135428038603	22.392475621179774
78-79	22.160871741948345	27.807743589357287	27.55246519945091	22.478919469243458
80-81	22.197795437743252	27.79287089253537	27.525251988943133	22.484081680778242
82-83	22.261277198432676	27.744443311450258	27.605988459306367	22.388291030810702
84-85	22.292597331186588	27.70783692900774	27.61462821936549	22.38493752044018
86-87	22.216833481967633	27.67869329470205	27.67083110331389	22.43364212001643
88-89	22.291925414883334	27.664368355079365	27.617300107346885	22.426406122690416
90-91	22.256016139879787	27.698797851765345	27.69300298117351	22.35218302718136
92-93	22.328938220934653	27.58862535720546	27.71054886058598	22.37188756127391
94-95	22.205526578058212	27.574143692564746	27.75605265076934	22.464277078607697
96-97	22.210446701530703	27.619322919127903	27.77799964196272	22.392230737378675
98-99	22.41632959652056	28.195449871489153	27.83821611570844	21.550004416281848
100-101	22.950286908764948	30.939086880849754	25.01874608916169	21.09188012122361
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2244.0
1	1914.5
2	2273.0
3	3142.5
4	4242.0
5	6482.5
6	8368.5
7	10226.5
8	11878.0
9	12252.0
10	11447.0
11	10672.0
12	11983.5
13	16089.0
14	25659.0
15	38551.5
16	48665.5
17	54299.5
18	55085.0
19	51324.5
20	45910.5
21	40950.0
22	38032.5
23	39555.0
24	45993.0
25	57524.5
26	72935.5
27	89493.0
28	107806.0
29	128020.5
30	147071.5
31	165796.0
32	185557.0
33	205716.5
34	225696.0
35	244124.5
36	264053.5
37	280185.0
38	290925.0
39	301060.0
40	311902.0
41	321292.0
42	324620.0
43	329149.0
44	334261.5
45	337374.5
46	338640.0
47	336680.0
48	333325.5
49	329333.0
50	324253.5
51	314576.0
52	300566.5
53	284325.0
54	266776.0
55	248811.0
56	228782.0
57	206199.0
58	183923.0
59	161002.0
60	136241.0
61	111009.0
62	87902.5
63	67968.0
64	50829.5
65	38191.5
66	29266.0
67	22678.0
68	17474.5
69	13079.0
70	9505.0
71	6805.0
72	4822.5
73	3310.0
74	2211.5
75	1416.0
76	917.5
77	566.0
78	361.5
79	231.0
80	148.5
81	102.5
82	74.5
83	49.5
84	29.0
85	18.0
86	12.5
87	11.0
88	10.0
89	8.0
90	6.0
91	5.0
92	4.5
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005309487516067479
3	0.0
4	1.1958305216368194E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	2.7504101997646846E-4
10-11	9.028520438357987E-4
12-13	0.0
14-15	1.1958305216368194E-5
16-17	0.0013512884894496058
18-19	0.0
20-21	0.0
22-23	6.697187904615541E-4
24-25	0.0024639606876268386
26-27	0.007549292182073853
28-29	0.36480575100608675
30-31	0.3355581276074522
32-33	0.33396322311182514
34-35	0.18745854085478975
36-37	0.050048095736446245
38-39	0.04868758662847184
40-41	0.02888584825182834
42-43	0.02843305600739432
44-45	0.016903982769612625
46-47	0.012074234274015494
48-49	0.012189364339530216
50-51	0.010408034542925736
52-53	0.00872388143241501
54-55	0.0018471771374172759
56-57	0.005638947287604281
58-59	0.004533056623609437
60-61	0.007561343726526529
62-63	0.002894596097307208
64-65	3.9873192434987603E-4
66-67	6.617908373898724E-4
68-69	0.0012471252385213146
70-71	0.003743904412135678
72-73	0.0017918925505334782
74-75	0.0011282433003458171
76-77	4.443928693866999E-4
78-79	1.4486325054012262E-4
80-81	0.0012303155913281206
82-83	0.0013181065858010744
84-85	0.0
86-87	0.004196022413121886
88-89	0.0017863993824731679
90-91	0.005281908670517178
92-93	0.014256644009629931
94-95	0.0016631399570654023
96-97	8.133513246646348E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	394.0
22-23	908.0
24-25	1980.0
26-27	2892.0
28-29	5618.0
30-31	15242.0
32-33	21314.0
34-35	31841.0
36-37	42695.0
38-39	49475.0
40-41	53787.0
42-43	65571.0
44-45	79164.0
46-47	77531.0
48-49	68083.0
50-51	60199.0
52-53	56098.0
54-55	61375.0
56-57	63415.0
58-59	63491.0
60-61	63776.0
62-63	62545.0
64-65	68194.0
66-67	72041.0
68-69	71936.0
70-71	70223.0
72-73	68831.0
74-75	70224.0
76-77	71731.0
78-79	75221.0
80-81	75785.0
82-83	74094.0
84-85	75775.0
86-87	78882.0
88-89	83160.0
90-91	88754.0
92-93	91684.0
94-95	101094.0
96-97	151573.0
98-99	699335.0
100-101	5326361.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.65422618530563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.73259680587275	78.62362965996236
2	6.605055911875897	11.447120179085681
3	1.521723306294064	3.955912667251708
4	0.5264352208477953	1.824713467970247
5	0.24251357570410761	1.050741312103549
6	0.1325056312350781	0.6889303763922694
7	0.06510291649865	0.3949009996117958
8	0.04051696400089323	0.280877293030023
9	0.034013230139535396	0.26526511240237394
>10	0.09710680938350094	1.2818204772362087
>50	0.0020643182330683436	0.11399095742524488
>100	3.2277338634925515E-4	0.045595507551268746
>500	4.25365283523167E-5	0.026501989977179674
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3916610432736388E-5	0.0	0.0	0.0
3	0.0	2.3916610432736388E-5	0.0	0.0	0.0
4	0.0	4.7833220865472776E-5	0.0	0.0	0.0
5	0.0	7.174983129820916E-5	0.0	0.0	0.0
6	4.7833220865472776E-5	7.174983129820916E-5	0.0	1.1958305216368194E-5	1.1958305216368194E-5
7	4.7833220865472776E-5	9.566644173094555E-5	0.0	1.1958305216368194E-5	1.1958305216368194E-5
8	4.7833220865472776E-5	1.0762474694731375E-4	0.0	1.1958305216368194E-5	2.3916610432736388E-5
9	4.7833220865472776E-5	2.8699932519283666E-4	0.0	5.979152608184097E-5	2.3916610432736388E-5
10-11	4.7833220865472776E-5	2.8699932519283666E-4	0.0	6.577067869002507E-5	3.587491564910458E-5
12-13	4.7833220865472776E-5	2.8699932519283666E-4	0.0	7.174983129820916E-5	4.185406825728868E-5
14-15	4.7833220865472776E-5	3.766866143155981E-4	0.0	8.370813651457736E-5	5.381237347365687E-5
16-17	1.1958305216368194E-4	3.826657669237822E-4	0.0	9.566644173094555E-5	5.979152608184097E-5
18-19	1.3154135738005013E-4	4.30498987789255E-4	0.0	1.6143712042097062E-4	6.577067869002507E-5
20-21	1.3154135738005013E-4	4.90290513871096E-4	0.0	2.2720779911099569E-4	7.174983129820916E-5
22-23	1.3154135738005013E-4	5.500820399529369E-4	0.0	4.6039475083017547E-4	8.968728912276145E-5
24-25	1.3154135738005013E-4	5.739986503856733E-4	0.0	0.001548600525519681	1.1360389955549784E-4
26-27	1.3154135738005013E-4	5.979152608184097E-4	0.0	0.004765384628722725	1.1958305216368194E-4
28-29	1.3154135738005013E-4	5.979152608184097E-4	0.0	0.02065199310866787	1.1958305216368194E-4
30-31	1.3154135738005013E-4	6.577067869002507E-4	0.0	0.02817974624237165	1.1958305216368194E-4
32-33	1.3154135738005013E-4	6.816233973329871E-4	0.0	0.030338220333926108	1.3752050998823423E-4
34-35	1.3154135738005013E-4	7.593523812393804E-4	0.0	0.03343542138496547	1.6741627302915472E-4
36-37	1.3154135738005013E-4	8.430605177539576E-4	0.0	0.038176889403255455	1.6741627302915472E-4
38-39	1.3154135738005013E-4	8.968728912276145E-4	0.0	0.0453040393122109	1.793745782455229E-4
40-41	1.3154135738005013E-4	9.9253933295856E-4	0.0	0.05998285896530286	1.793745782455229E-4
42-43	1.3154135738005013E-4	0.0010044976381749283	0.0	0.06737309158901841	1.793745782455229E-4
44-45	1.3154135738005013E-4	0.0010702683168649532	0.0	0.07745394288641679	1.793745782455229E-4
46-47	1.3154135738005013E-4	0.0011360389955549783	0.0	0.08838383385417732	2.032911886782593E-4
48-49	1.3154135738005013E-4	0.0011360389955549783	0.0	0.09852447667765754	2.511244095437321E-4
50-51	1.4349966259641833E-4	0.0012735595055432126	0.0	0.10829441203943035	2.6308271476010027E-4
52-53	1.4349966259641833E-4	0.001291496963367765	0.0	0.1205337374283832	2.8102017258465256E-4
54-55	1.4349966259641833E-4	0.0013154135738005013	0.0	0.1441812859937513	3.1689508823375714E-4
56-57	1.4349966259641833E-4	0.0013512884894496058	0.0	0.1695209347472355	3.2885339345012533E-4
58-59	1.4349966259641833E-4	0.0013632467946659741	0.0	0.1959248726649765	3.3483254605830943E-4
60-61	1.4349966259641833E-4	0.0013632467946659741	0.0	0.21390418455778604	3.3483254605830943E-4
62-63	1.733954256373388E-4	0.0013991217103150786	0.0	0.22462480518426015	3.467908512746776E-4
64-65	1.913328834618911E-4	0.0016382878146424425	0.0	0.23458607342949483	3.467908512746776E-4
66-67	2.3916610432736388E-4	0.0016382878146424425	0.0	0.2413724116397838	3.467908512746776E-4
68-69	2.511244095437321E-4	0.0016502461198588108	0.0	0.24703466915973415	3.467908512746776E-4
70-71	2.5710356215191617E-4	0.0016562252724669948	0.0	0.25240394820188344	3.527700038828617E-4
72-73	2.6906186736828436E-4	0.0016681835776833631	0.0	0.25674481299542506	4.006032247483345E-4
74-75	2.7504101997646846E-4	0.0017100376459406516	0.0	0.2602725130342537	4.065823773565186E-4
76-77	2.7504101997646846E-4	0.0017578708668061244	0.0	0.263172402049223	4.065823773565186E-4
78-79	2.9297847780102075E-4	0.0017817874772388608	0.0	0.26604837445375956	4.065823773565186E-4
80-81	2.9895763040920485E-4	0.001793745782455229	0.0	0.26722626751757184	4.245198351810709E-4
82-83	2.9895763040920485E-4	0.0018116832402797814	0.0	0.267686662268402	4.30498987789255E-4
84-85	2.9895763040920485E-4	0.0018296206981043336	0.0	0.2679676824409867	4.30498987789255E-4
86-87	2.9895763040920485E-4	0.0018654956137534383	0.0	0.26806334888271766	4.30498987789255E-4
88-89	3.1091593562557304E-4	0.001979099513308936	0.0	0.2680932446457585	4.364781403974391E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10880	0.0	52.445515	1
GTATCAA	20640	0.0	38.59613	1
TCAACGC	25710	0.0	30.533806	4
ATCAACG	25840	0.0	30.396574	3
CAACGCA	26480	0.0	29.678612	5
TATCAAC	26630	0.0	29.625196	2
AACGCAG	27330	0.0	28.833876	6
ACGCAGA	31370	0.0	25.134136	7
CGCAGAG	32250	0.0	24.46158	8
GTGGTAT	5475	0.0	23.768213	1
TGGTATC	5185	0.0	22.703405	2
GCAGAGT	36890	0.0	21.268784	9
GTACATG	25250	0.0	19.95364	1
GAGTACT	22120	0.0	19.941101	12-13
TACATGG	25270	0.0	19.649876	2
ACATGGG	25415	0.0	19.115553	3
AGAGTAC	33610	0.0	18.899672	10-11
CAGAGTA	36130	0.0	18.629944	10-11
GTACTTT	24350	0.0	18.097727	14-15
AGTACTT	24170	0.0	17.559105	12-13
>>END_MODULE