FastQCFastQC Report
Fri 27 May 2016
SRR2139620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7466520
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.052.757571
GTATCAA184550.039.0151521
TCAACGC232150.030.3876954
ATCAACG232850.030.3698353
TATCAAC239700.029.6459142
CAACGCA238850.029.5360815
AACGCAG243650.028.9366516
ACGCAGA277500.025.4223167
CGCAGAG284300.024.8293048
GTGGTAT47000.023.393681
TGGTATC45250.022.5965732
GCAGAGT323300.021.7282589
GTACATG233750.020.8466071
TACATGG233200.020.0885622
GAGTACT191550.019.7661212-13
ACATGGG235650.019.5158213
AGAGTAC297350.019.28695310-11
CAGAGTA321200.018.85380710-11
GTACTTT213050.017.71123514-15
AGTACTT208800.017.28283512-13
Produced by FastQC (version 0.11.5)