##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7466520
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95467433288868	34.0	31.0	34.0	31.0	34.0
2	33.078637437521095	34.0	33.0	34.0	31.0	34.0
3	33.13690943036381	34.0	33.0	34.0	31.0	34.0
4	36.48488010478777	37.0	37.0	37.0	35.0	37.0
5	36.451905974938796	37.0	37.0	37.0	35.0	37.0
6	36.4810270380311	37.0	37.0	37.0	35.0	37.0
7	36.45607699436953	37.0	37.0	37.0	35.0	37.0
8	36.44298588900853	37.0	37.0	37.0	35.0	37.0
9	38.24170403347209	39.0	39.0	39.0	37.0	39.0
10-11	38.18439266217729	39.0	39.0	39.0	37.0	39.0
12-13	38.155454147313606	39.0	39.0	39.0	37.0	39.0
14-15	39.6753621499708	41.0	40.0	41.0	37.0	41.0
16-17	39.64826458644723	41.0	40.0	41.0	37.0	41.0
18-19	39.65860802622909	41.0	40.0	41.0	37.0	41.0
20-21	39.65340527900116	41.0	40.0	41.0	37.0	41.0
22-23	39.59626704779208	41.0	40.0	41.0	37.0	41.0
24-25	39.53203129596028	41.0	40.0	41.0	37.0	41.0
26-27	39.48383637342455	41.0	39.5	41.0	37.0	41.0
28-29	39.318045822223255	41.0	39.0	41.0	36.0	41.0
30-31	39.23559158827227	41.0	39.0	41.0	36.0	41.0
32-33	39.132668834882324	41.0	39.0	41.0	35.5	41.0
34-35	39.03565331708798	40.5	39.0	41.0	35.0	41.0
36-37	39.12719791886643	40.5	39.0	41.0	35.0	41.0
38-39	39.153803508094526	41.0	39.0	41.0	35.0	41.0
40-41	39.146592311668414	41.0	39.0	41.0	35.0	41.0
42-43	39.105299215224235	41.0	39.0	41.0	35.0	41.0
44-45	39.03812400438268	40.5	39.0	41.0	35.0	41.0
46-47	38.95322185268533	40.0	39.0	41.0	35.0	41.0
48-49	38.878027049804615	40.0	38.5	41.0	35.0	41.0
50-51	38.78728976563286	40.0	38.0	41.0	35.0	41.0
52-53	38.68388658473572	40.0	38.0	41.0	35.0	41.0
54-55	38.56091203317658	40.0	38.0	41.0	35.0	41.0
56-57	38.392137033580255	40.0	37.5	41.0	34.0	41.0
58-59	38.23092947422803	40.0	37.0	41.0	34.0	41.0
60-61	38.03781281737362	40.0	36.5	41.0	34.0	41.0
62-63	37.78726703788624	39.0	36.0	41.0	34.0	41.0
64-65	37.49170495738333	39.0	35.5	40.5	33.5	41.0
66-67	37.17089607810969	38.5	35.0	40.0	33.0	41.0
68-69	36.859489668516915	37.5	35.0	40.0	33.0	41.0
70-71	36.53441919281053	37.0	35.0	39.5	33.0	41.0
72-73	36.192695605916214	36.5	35.0	39.0	32.0	41.0
74-75	35.822988974097065	36.0	35.0	39.0	32.0	40.0
76-77	34.886665053760765	35.0	34.0	37.0	30.5	39.0
78-79	35.11621458620952	35.0	34.0	37.0	31.5	39.0
80-81	34.93290609424453	35.0	34.0	37.0	32.0	39.0
82-83	34.66382197662735	35.0	34.0	36.0	31.0	38.5
84-85	34.413531006497934	35.0	34.0	36.0	31.0	37.0
86-87	34.162696757935635	35.0	34.0	35.5	31.0	37.0
88-89	33.86159443388648	35.0	34.0	35.0	31.0	36.5
90-91	33.78214929687998	35.0	34.0	35.0	31.0	36.0
92-93	33.69617919294083	35.0	34.0	35.0	31.0	36.0
94-95	33.58953229702439	35.0	34.0	35.0	31.0	35.5
96-97	33.553914961479876	35.0	33.0	35.0	31.0	35.0
98-99	33.552553938605314	35.0	33.0	35.0	31.0	35.0
100-101	32.48999282907391	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	10.0
20	31.0
21	153.0
22	638.0
23	2244.0
24	5502.0
25	11438.0
26	20309.0
27	31968.0
28	45615.0
29	61415.0
30	79486.0
31	100776.0
32	127471.0
33	169465.0
34	247585.0
35	378146.0
36	713550.0
37	1714091.0
38	2506447.0
39	1186553.0
40	63624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76689532750009	20.802845557038175	15.27516779242584	24.155091323035897
2	13.856693780295226	21.363574873662454	41.399644527069654	23.380086818972664
3	17.001005957664063	26.379234119469626	31.134463364912985	25.485296557953323
4	12.021214175230565	18.52127303801759	38.9674328739893	30.490079912762535
5	13.115968262042395	37.85746419649215	35.59270005010368	13.43386749136177
6	29.711885742709754	38.21992235469362	18.676043997489057	13.392147905107562
7	26.33219746235092	32.946914873427595	23.032533107471664	17.68835455674982
8	24.604139545598215	36.40728210732711	21.0702040575797	17.918374289494974
9	25.527884409593675	16.884733975829647	21.340153662317373	36.2472279522593
10-11	23.99178459403787	26.944770346322084	28.923619760904096	20.139825298735946
12-13	24.926391411259864	24.553754627323038	28.987480111216467	21.532373850200628
14-15	22.089098291867774	25.925357710247265	26.75786089312314	25.227683104761816
16-17	21.542159121881117	29.070223125824107	27.416477653599486	21.97114009869529
18-19	21.59217413199188	28.1066480770158	29.271762481048736	21.029415309943587
20-21	22.436827158967645	27.213672862394777	29.24042451088419	21.10907546775339
22-23	22.149277781051932	27.129521235273003	29.082023820366786	21.639177163308275
24-25	21.87360845193141	27.28868295986929	29.16706855780561	21.670640030393688
26-27	21.792156519592755	27.450010579523376	29.09453350198036	21.663299398903508
28-29	21.721469384416526	27.446016578575495	29.09644799392133	21.736066043086648
30-31	21.966382976174025	27.338390088148444	29.0352218830065	21.660005052671032
32-33	21.817083443281202	27.497948089497292	28.918562221858878	21.766406245362628
34-35	21.920365999656557	27.52068252850726	28.947487528603855	21.61146394323233
36-37	21.917577696187156	27.443899975042267	28.853625740771804	21.784896587998773
38-39	21.80107520289148	27.59815266093505	28.90483154618797	21.6959405899855
40-41	22.053097461570704	27.469809899626856	28.737972632284	21.73912000651844
42-43	21.96669705690348	27.59417172620524	28.484861197716654	21.954270019174622
44-45	22.02261623803106	27.679668176962913	28.336597287034067	21.96111829797196
46-47	22.149884021312356	27.644508770058003	28.0234549517579	22.182152256871742
48-49	22.087188205664184	27.76270886488948	27.989571594807877	22.160531334638456
50-51	22.078637781043557	27.905309588940337	27.861434069769945	22.15461856024616
52-53	22.27097474054699	27.796153887188723	27.710902260083643	22.221969112180645
54-55	22.235523277338668	27.704099098896286	27.7494367829431	22.310940840821956
56-57	22.27412732547077	27.865858314662358	27.683720674159655	22.17629368570722
58-59	22.153428646282833	27.898832768124603	27.753605166509022	22.19413341908354
60-61	22.291046447754066	27.859719276863487	27.698639471936954	22.150594803445493
62-63	22.22567289416614	27.94498522904717	27.65053456263334	22.178807314153353
64-65	22.227650297511445	27.868218917843528	27.654643673400315	22.249487111244708
66-67	22.280503950393815	27.911343928868448	27.485109583679858	22.323042537057873
68-69	22.209798482969052	27.899741530808665	27.58522268658339	22.305237299638897
70-71	22.216221244858307	27.68052182821725	27.64392591702468	22.459331009899767
72-73	22.27602398400856	27.716892945632022	27.541741490374072	22.465341579985346
74-75	22.196192182321067	27.822163488316896	27.489352160518493	22.492292168843544
76-77	22.295654112691416	27.739094962681186	27.483221613965043	22.482029310662355
78-79	22.249819394952798	27.738759872399488	27.44633392046884	22.565086812178865
80-81	22.298116473028667	27.744502737437838	27.40185823861554	22.555522550917953
82-83	22.354434271222267	27.66417399944856	27.506966481707206	22.47442524762197
84-85	22.36534475687971	27.666463617823535	27.494494478450488	22.473697146846273
86-87	22.293483450220954	27.64503085575047	27.568714910794384	22.492770783234192
88-89	22.381027877206755	27.60139499891942	27.5262657913766	22.491311332497226
90-91	22.362612850278595	27.630526368212223	27.558479455790273	22.44838132571891
92-93	22.395678071344104	27.520303853158122	27.612154196965566	22.471863878532204
94-95	22.278527847218694	27.523349311690176	27.654621547235912	22.54350129385522
96-97	22.28158166301137	27.54282003631408	27.710075616955542	22.465522683719005
98-99	22.524617611169443	28.129212898587742	27.739076969645378	21.607092520597444
100-101	23.04507393513	30.855967957389336	24.913975913044123	21.184982194436543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1817.0
1	1552.5
2	1854.5
3	2558.5
4	3550.0
5	5700.5
6	7428.0
7	8910.0
8	10158.0
9	10342.5
10	9666.5
11	9040.5
12	10149.5
13	13726.5
14	22269.5
15	34012.0
16	43410.5
17	48600.5
18	49217.5
19	45756.0
20	40607.0
21	35902.0
22	33429.0
23	34753.5
24	40098.0
25	49634.5
26	62477.5
27	77229.0
28	93267.0
29	110102.0
30	126016.0
31	142400.0
32	160938.0
33	180127.5
34	197995.5
35	213935.5
36	233086.0
37	248568.0
38	258208.5
39	268358.0
40	278372.0
41	286927.5
42	291284.5
43	295317.0
44	300592.5
45	304394.5
46	305512.5
47	303712.5
48	300317.0
49	297111.0
50	292421.0
51	283756.0
52	271218.0
53	256563.5
54	241592.5
55	225912.5
56	208283.0
57	188804.5
58	168383.0
59	145906.5
60	122837.0
61	100944.5
62	79816.5
63	61380.0
64	46354.5
65	34733.5
66	26433.5
67	20360.5
68	15536.5
69	11455.0
70	8374.0
71	6132.5
72	4326.0
73	2986.0
74	2004.5
75	1310.5
76	858.5
77	546.0
78	348.0
79	230.5
80	151.0
81	96.0
82	67.5
83	49.0
84	34.0
85	25.0
86	21.0
87	16.5
88	13.0
89	9.0
90	5.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.017936066601308E-5
2	0.005491179291021788
3	4.017936066601308E-5
4	4.017936066601308E-5
5	4.017936066601308E-5
6	4.017936066601308E-5
7	4.017936066601308E-5
8	0.0
9	2.410761639960785E-4
10-11	8.035872133202617E-4
12-13	0.0
14-15	2.678624044400872E-5
16-17	0.0014062776233104578
18-19	0.0
20-21	0.0
22-23	8.103466669738394E-4
24-25	0.0028399500597461194
26-27	0.00761078142224334
28-29	0.3637432560922622
30-31	0.3345170230594745
32-33	0.33349658466340587
34-35	0.1879919398312516
36-37	0.05209684026637505
38-39	0.05036538908437602
40-41	0.03095163536370261
42-43	0.030123556938834076
44-45	0.0179861471181948
46-47	0.012614719276113294
48-49	0.012579432053897013
50-51	0.011346816298692849
52-53	0.009363393743681602
54-55	0.0022294343576458597
56-57	0.006098215382525018
58-59	0.004930984304927936
60-61	0.008247606240222251
62-63	0.0028975796427164195
64-65	4.845871381941815E-4
66-67	8.484713489188602E-4
68-69	0.0016289647535260524
70-71	0.004382271637056832
72-73	0.002354405975498114
74-75	0.001582291816911503
76-77	6.505620695648431E-4
78-79	1.7857545811504513E-4
80-81	0.0012639914206171938
82-83	0.0014026555007542385
84-85	0.0
86-87	0.004091521738798271
88-89	0.0017959192932774015
90-91	0.005223672429770336
92-93	0.014452728497681692
94-95	0.001613315662149114
96-97	9.117725980786945E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	357.0
22-23	759.0
24-25	1714.0
26-27	2546.0
28-29	5091.0
30-31	14305.0
32-33	19358.0
34-35	28748.0
36-37	39040.0
38-39	44424.0
40-41	48102.0
42-43	58750.0
44-45	70652.0
46-47	69752.0
48-49	60467.0
50-51	53314.0
52-53	50602.0
54-55	54493.0
56-57	56364.0
58-59	56275.0
60-61	56798.0
62-63	56072.0
64-65	61158.0
66-67	64565.0
68-69	64516.0
70-71	63178.0
72-73	61192.0
74-75	62551.0
76-77	64540.0
78-79	67922.0
80-81	67846.0
82-83	66032.0
84-85	67351.0
86-87	70533.0
88-89	74466.0
90-91	79624.0
92-93	82074.0
94-95	90224.0
96-97	135988.0
98-99	625687.0
100-101	4749016.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.14519895461626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.91066771077037	79.22428224752092
2	6.592116288102172	11.489425709172604
3	1.4367949298967935	3.7562934006852005
4	0.49751989669778207	1.7342588152643341
5	0.2273443225316025	0.9905983109109475
6	0.11804336135045937	0.6172147326094465
7	0.06622286461936279	0.40397032988192927
8	0.04278212305104898	0.298260529998763
9	0.022219742601404524	0.17427095007477839
>10	0.08469708542451906	1.1372973718021036
>50	0.0011388911194684902	0.06402892864971739
>100	4.070694082504182E-4	0.07328842822618378
>500	2.2857215366095578E-5	0.015337603670827218
>1k	2.285721152656505E-5	0.021472641532206196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3393120222004361E-5	2.6786240444008722E-5
2	0.0	1.3393120222004361E-5	0.0	2.6786240444008722E-5	2.6786240444008722E-5
3	0.0	1.3393120222004361E-5	0.0	2.6786240444008722E-5	2.6786240444008722E-5
4	0.0	4.017936066601308E-5	0.0	5.3572480888017444E-5	2.6786240444008722E-5
5	1.3393120222004361E-5	5.3572480888017444E-5	0.0	6.69656011100218E-5	2.6786240444008722E-5
6	8.035872133202617E-5	5.3572480888017444E-5	0.0	6.69656011100218E-5	4.017936066601308E-5
7	8.035872133202617E-5	6.69656011100218E-5	0.0	6.69656011100218E-5	4.017936066601308E-5
8	9.375184155403053E-5	8.035872133202617E-5	0.0	6.69656011100218E-5	4.017936066601308E-5
9	9.375184155403053E-5	1.741105628860567E-4	0.0	6.69656011100218E-5	4.017936066601308E-5
10-11	9.375184155403053E-5	1.741105628860567E-4	0.0	8.705528144302835E-5	4.017936066601308E-5
12-13	9.375184155403053E-5	1.808071229970589E-4	0.0	9.375184155403053E-5	4.017936066601308E-5
14-15	1.0044840166503271E-4	2.0089680333006541E-4	0.0	9.375184155403053E-5	4.017936066601308E-5
16-17	1.4732432244204797E-4	2.0089680333006541E-4	0.0	1.2053808199803925E-4	4.017936066601308E-5
18-19	1.5402088255305016E-4	2.3437960388507633E-4	0.0	1.4062776233104578E-4	4.017936066601308E-5
20-21	1.6071744266405233E-4	2.745589645510894E-4	0.0	1.9420024321906322E-4	4.017936066601308E-5
22-23	1.6071744266405233E-4	3.750073662161221E-4	0.0	4.888488881031592E-4	4.017936066601308E-5
24-25	2.1428992355206978E-4	4.2857984710413955E-4	0.0	0.001520119145197495	4.017936066601308E-5
26-27	2.2768304377407414E-4	4.419729673261439E-4	0.0	0.004540267755259479	4.017936066601308E-5
28-29	2.2768304377407414E-4	4.553660875481482E-4	0.0	0.021864268762422118	4.017936066601308E-5
30-31	2.410761639960785E-4	5.089385684361657E-4	0.0	0.029726030332738677	4.017936066601308E-5
32-33	2.678624044400872E-4	5.156351285471679E-4	0.0	0.03174839148626134	4.017936066601308E-5
34-35	2.879520847730938E-4	5.826007296571897E-4	0.0	0.03490247129854336	4.017936066601308E-5
36-37	3.080417651061003E-4	6.428697706562093E-4	0.0	0.039476221854357854	4.017936066601308E-5
38-39	3.080417651061003E-4	6.495663307672114E-4	0.0	0.04670181021412921	4.017936066601308E-5
40-41	3.2143488532810466E-4	7.232284919882354E-4	0.0	0.06039627564112866	4.017936066601308E-5
42-43	3.2143488532810466E-4	7.567112925432463E-4	0.0	0.06745444999812497	4.6875920777015263E-5
44-45	3.2143488532810466E-4	8.638562543192812E-4	0.0	0.07733187616185318	5.3572480888017444E-5
46-47	3.2143488532810466E-4	9.442149756513074E-4	0.0	0.08866245586966887	6.69656011100218E-5
48-49	3.34828005550109E-4	9.509115357623097E-4	0.0	0.09879435131761516	6.69656011100218E-5
50-51	3.34828005550109E-4	0.001151808339092375	0.0	0.10855793595945634	6.69656011100218E-5
52-53	3.34828005550109E-4	0.0011919876997583882	0.0	0.1200827159104911	7.366216122102399E-5
54-55	3.34828005550109E-4	0.0011919876997583882	0.0	0.1438086283837718	8.035872133202617E-5
56-57	3.482211257721134E-4	0.001225470500313399	0.0	0.16878679759780996	8.035872133202617E-5
58-59	3.482211257721134E-4	0.0012522567407574077	0.0	0.19476945082849842	8.705528144302835E-5
60-61	3.683108061051199E-4	0.0013125257817564275	0.0	0.21287025280853733	9.375184155403053E-5
62-63	3.750073662161221E-4	0.0013259189019784318	0.0	0.22316956225925866	1.339312022200436E-4
64-65	3.750073662161221E-4	0.0014732432244204798	0.0	0.2330603815432089	1.339312022200436E-4
66-67	3.750073662161221E-4	0.001486636344642484	0.0	0.23911407188355485	1.339312022200436E-4
68-69	4.0179360666013083E-4	0.0015067260249754908	0.0	0.24497356198068176	1.339312022200436E-4
70-71	4.0179360666013083E-4	0.001520119145197495	0.0	0.25026384446837346	1.339312022200436E-4
72-73	4.2857984710413955E-4	0.001526815705308497	0.0	0.2546768775815239	1.4732432244204797E-4
74-75	4.2857984710413955E-4	0.0015803881861965146	0.0	0.25807873011791305	1.4732432244204797E-4
76-77	4.419729673261439E-4	0.0016875331479725495	0.0	0.26148727921441317	1.4732432244204797E-4
78-79	4.419729673261439E-4	0.0017277125086385626	0.0	0.264031972056594	1.4732432244204797E-4
80-81	4.553660875481483E-4	0.0017277125086385626	0.0	0.26529092535746235	1.4732432244204797E-4
82-83	4.553660875481483E-4	0.0017277125086385626	0.0	0.26561236024279045	1.6071744266405233E-4
84-85	4.553660875481483E-4	0.0017411056288605669	0.0	0.26575298800512154	1.6071744266405233E-4
86-87	4.553660875481483E-4	0.0017411056288605669	0.0	0.26583334672645353	1.6071744266405233E-4
88-89	4.687592077701526E-4	0.0019085196316356214	0.0	0.2658668295270086	1.6071744266405233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9950	0.0	52.75757	1
GTATCAA	18455	0.0	39.015152	1
TCAACGC	23215	0.0	30.387695	4
ATCAACG	23285	0.0	30.369835	3
TATCAAC	23970	0.0	29.645914	2
CAACGCA	23885	0.0	29.536081	5
AACGCAG	24365	0.0	28.936651	6
ACGCAGA	27750	0.0	25.422316	7
CGCAGAG	28430	0.0	24.829304	8
GTGGTAT	4700	0.0	23.39368	1
TGGTATC	4525	0.0	22.596573	2
GCAGAGT	32330	0.0	21.728258	9
GTACATG	23375	0.0	20.846607	1
TACATGG	23320	0.0	20.088562	2
GAGTACT	19155	0.0	19.76612	12-13
ACATGGG	23565	0.0	19.515821	3
AGAGTAC	29735	0.0	19.286953	10-11
CAGAGTA	32120	0.0	18.853807	10-11
GTACTTT	21305	0.0	17.711235	14-15
AGTACTT	20880	0.0	17.282835	12-13
>>END_MODULE