FastQCFastQC Report
Fri 27 May 2016
SRR2139619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13284874
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187150.051.8572161
GTATCAA350400.038.254841
TCAACGC437750.030.0722894
ATCAACG442150.029.8018863
CAACGCA450200.029.2693315
TATCAAC454600.029.1374572
AACGCAG464850.028.3745576
GTGGTAT87800.025.4858191
ACGCAGA533450.024.6935337
TGGTATC86000.024.4241942
CGCAGAG546550.024.1173538
GCAGAGT618200.021.1487799
GTACATG414250.020.4581681
GAGTACT372550.019.87111312-13
TACATGG416200.019.8473362
ACATGGG416350.019.0777153
AGAGTAC568300.019.02675210-11
CAGAGTA608450.018.53561810-11
GTACTTT410000.018.05113414-15
AGTACTT406200.017.81868212-13
Produced by FastQC (version 0.11.5)