##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139619_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13284874
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.776466152407615	34.0	31.0	34.0	31.0	34.0
2	32.94115856876023	34.0	31.0	34.0	31.0	34.0
3	33.00092503700073	34.0	31.0	34.0	31.0	34.0
4	36.41700252482636	37.0	37.0	37.0	35.0	37.0
5	36.37021096323533	37.0	37.0	37.0	35.0	37.0
6	36.397388864960256	37.0	37.0	37.0	35.0	37.0
7	36.36940485848793	37.0	37.0	37.0	35.0	37.0
8	36.3548543253026	37.0	37.0	37.0	35.0	37.0
9	38.119216034717375	39.0	39.0	39.0	37.0	39.0
10-11	38.047217873500344	39.0	38.0	39.0	36.0	39.0
12-13	38.01497974312741	39.0	38.0	39.0	35.0	39.0
14-15	39.47220120416648	41.0	39.0	41.0	36.5	41.0
16-17	39.44155906183228	41.0	39.0	41.0	36.5	41.0
18-19	39.449076445888764	41.0	39.0	41.0	36.5	41.0
20-21	39.437846855481766	41.0	39.0	41.0	36.5	41.0
22-23	39.37268986076404	41.0	39.0	41.0	36.0	41.0
24-25	39.2974489335659	40.5	39.0	41.0	36.0	41.0
26-27	39.24049013679817	40.0	39.0	41.0	36.0	41.0
28-29	39.075567009266024	40.0	39.0	41.0	36.0	41.0
30-31	38.983296686425945	40.0	39.0	41.0	35.5	41.0
32-33	38.86813649594616	40.0	38.0	41.0	35.0	41.0
34-35	38.761051479508694	40.0	38.0	41.0	35.0	41.0
36-37	38.85738644299131	40.0	38.0	41.0	35.0	41.0
38-39	38.887048172680025	40.0	38.0	41.0	35.0	41.0
40-41	38.87838306735513	40.0	38.0	41.0	35.0	41.0
42-43	38.831255823471594	40.0	38.0	41.0	35.0	41.0
44-45	38.757519289957855	40.0	38.0	41.0	35.0	41.0
46-47	38.66649668054191	40.0	38.0	41.0	35.0	41.0
48-49	38.591059049915046	40.0	38.0	41.0	35.0	41.0
50-51	38.50126101554217	40.0	38.0	41.0	34.0	41.0
52-53	38.390760753392215	40.0	38.0	41.0	34.0	41.0
54-55	38.26783499707406	40.0	37.5	41.0	34.0	41.0
56-57	38.09585924845978	40.0	37.0	41.0	34.0	41.0
58-59	37.939149680957435	40.0	37.0	41.0	34.0	41.0
60-61	37.7546418083148	39.0	36.0	41.0	33.0	41.0
62-63	37.50245071389105	39.0	36.0	40.5	33.0	41.0
64-65	37.203229769402235	38.5	35.0	40.0	33.0	41.0
66-67	36.87558987791543	38.0	35.0	40.0	32.0	41.0
68-69	36.56778953477563	37.0	35.0	40.0	32.0	41.0
70-71	36.2444173017872	37.0	35.0	39.0	32.0	41.0
72-73	35.90761102657039	36.0	35.0	39.0	31.0	40.0
74-75	35.544095768099204	36.0	34.0	38.5	31.0	40.0
76-77	34.617128634911225	35.0	33.5	37.0	30.5	39.0
78-79	34.88278681885422	35.0	34.0	37.0	31.0	39.0
80-81	34.70963519594529	35.0	34.0	37.0	31.0	39.0
82-83	34.4417573883577	35.0	34.0	36.0	31.0	38.5
84-85	34.18901862556376	35.0	34.0	36.0	31.0	37.0
86-87	33.92826129968954	35.0	34.0	35.5	30.0	37.0
88-89	33.62161021050487	35.0	33.5	35.0	30.0	36.5
90-91	33.534425462973836	35.0	33.0	35.0	30.0	36.0
92-93	33.4472010604394	35.0	33.0	35.0	30.0	36.0
94-95	33.33487493297062	34.5	33.0	35.0	30.0	35.5
96-97	33.30229761782523	34.0	33.0	35.0	30.0	35.0
98-99	33.31400326759443	34.0	33.0	35.0	30.0	35.0
100-101	32.215807800283045	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	18.0
20	47.0
21	293.0
22	1488.0
23	4833.0
24	12129.0
25	24891.0
26	43348.0
27	68204.0
28	96121.0
29	129116.0
30	165606.0
31	208857.0
32	265406.0
33	352045.0
34	513549.0
35	786358.0
36	1479896.0
37	3271347.0
38	4095861.0
39	1702531.0
40	62924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76280084035448	21.865912124093047	15.995482078484772	24.3758049570677
2	13.281091565342509	21.928971289485258	41.33072653941935	23.459210605752883
3	16.296192586001972	26.877440276043345	31.56003784756644	25.26632929038824
4	11.724578411380767	18.9475019326497	39.10083425731678	30.227085398652754
5	12.923551378372538	37.88719046554464	35.768983813917636	13.420274342165184
6	29.44298288203046	38.501377883261185	18.724820039639077	13.330819195069276
7	25.880620143018323	33.07125074831362	23.31394109886351	17.734188009804537
8	24.390189925775736	36.6070163706483	21.094230927594797	17.908562775981167
9	25.28812558563029	16.8526406656855	21.554762604449948	36.30447114423427
10-11	23.848869764916326	27.138562808772036	28.95814123537833	20.054426190933302
12-13	24.76712613156888	24.77045698739785	29.08622242107829	21.376194459954984
14-15	21.932750128661375	26.097938610327702	26.992294167960807	24.97701709305012
16-17	21.333423964048208	29.22805928438093	27.6123108577817	21.826205893789158
18-19	21.35134288815987	28.20753512603883	29.622019749679225	20.819102236122074
20-21	22.17443818809075	27.274596157435493	29.613984737671938	20.93698091680182
22-23	21.90211951794132	27.190977190028644	29.46550762998605	21.441395662044
24-25	21.677711961593403	27.35709896739616	29.511340902405784	21.453848168604654
26-27	21.56645364448554	27.509523555398413	29.45259662987654	21.471426170239504
28-29	21.491063007642293	27.527101826775862	29.505341111458016	21.476494054123833
30-31	21.767154260114427	27.413090363137364	29.402130474386784	21.417624902361425
32-33	21.61048694676398	27.55461496486263	29.280954255468338	21.553943832905052
34-35	21.69284011480089	27.605455039266058	29.317252228710988	21.384452617222063
36-37	21.71680009866558	27.519717889531094	29.234637556260694	21.528844455542632
38-39	21.599752119161895	27.669633499057916	29.2444551797226	21.486159202057593
40-41	21.81033732234813	27.572493479487648	29.09252357433769	21.524645623826537
42-43	21.735483694724525	27.68080683694182	28.827386825778373	21.75632264255528
44-45	21.849820641807298	27.78203684593864	28.6071325909674	21.761009921286664
46-47	21.9578257897416	27.766250831214546	28.31255024078886	21.963373138254997
48-49	21.918514275191267	27.912096166020483	28.23054041215192	21.938849146636326
50-51	21.9298379445704	28.047453497844575	28.060931685223096	21.961776872361927
52-53	22.10728267927779	27.920421194318624	27.956502350471773	22.01579377593181
54-55	22.087635221470496	27.87006521174728	27.97020113318774	22.072098433594487
56-57	22.056265530779275	27.982170236507404	27.924136947726453	22.037427284986872
58-59	21.99721401557092	28.00131534405766	27.97380961176625	22.027661028605173
60-61	22.094853022439178	28.01922583685564	27.912399413632826	21.973521727072356
62-63	22.072605724948975	28.056565353510756	27.906250462481484	21.964578459058785
64-65	22.06256583005091	27.962104275648556	27.89518677292293	22.0801431213776
66-67	22.12320874522172	28.044222276581817	27.709321723903912	22.12324725429255
68-69	22.039825389499132	28.034073907592372	27.815847505412133	22.110253197496363
70-71	22.055850342365233	27.814110342367847	27.865065769720825	22.264973545546095
72-73	22.10458796653875	27.86330079238568	27.75404282882213	22.27806841225344
74-75	21.994728241920814	27.96365355183099	27.76537544951088	22.276242756737314
76-77	22.153295373722397	27.835551442817597	27.724082506331705	22.287070677128302
78-79	22.089083423012674	27.85464387672622	27.69773585856442	22.358536841696683
80-81	22.108650867587176	27.916894884873837	27.648568102286553	22.325886145252433
82-83	22.16478605221324	27.82426416110921	27.728962266974776	22.28198751970277
84-85	22.18737363980294	27.810493619445005	27.726169414132595	22.275963326619465
86-87	22.109332292889103	27.830044232489907	27.76892224860154	22.291701226019452
88-89	22.168494263376992	27.744024735629214	27.755591438293997	22.331889562699796
90-91	22.171864365419864	27.77572530516494	27.78568014925152	22.266730180163673
92-93	22.22630685248746	27.67108823498441	27.84417361851677	22.258431294011363
94-95	22.082975092227993	27.66359281027613	27.886488530518626	22.36694356697725
96-97	22.09200835026618	27.736592579322622	27.90182588451634	22.269573185894856
98-99	22.31222972563118	28.310768396929	27.930144111273446	21.446857766166374
100-101	22.80151491299039	31.024772857126187	25.17294272229445	21.000769507588974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5482.0
1	4444.0
2	4564.5
3	5990.0
4	7661.5
5	11265.5
6	14597.0
7	18300.0
8	21567.0
9	22168.5
10	20480.5
11	18647.0
12	20268.5
13	27079.5
14	44312.5
15	67916.5
16	85994.5
17	94718.5
18	94905.5
19	88701.5
20	79678.0
21	71096.5
22	65909.0
23	68079.5
24	78401.0
25	96232.0
26	119725.5
27	146027.5
28	175083.0
29	206259.0
30	235332.0
31	264668.5
32	297944.0
33	331634.0
34	361870.5
35	389159.0
36	422027.5
37	448897.0
38	466316.0
39	483421.5
40	498953.5
41	511624.0
42	515367.0
43	519851.5
44	526566.5
45	529218.0
46	529431.5
47	524939.0
48	519945.0
49	513686.0
50	503538.5
51	487859.0
52	465262.5
53	440044.5
54	413218.5
55	383874.0
56	353116.5
57	320799.0
58	285998.0
59	249197.0
60	210237.0
61	171957.0
62	136507.5
63	104889.5
64	78190.0
65	58268.5
66	44620.0
67	34467.0
68	26392.5
69	19432.5
70	13802.5
71	9754.0
72	6739.0
73	4553.5
74	3020.5
75	1957.5
76	1235.0
77	758.5
78	492.0
79	297.0
80	201.0
81	139.5
82	99.5
83	66.5
84	45.0
85	33.5
86	26.5
87	21.5
88	13.5
89	8.5
90	5.5
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.0109431222305913E-5
2	0.004606742977012804
3	3.0109431222305913E-5
4	3.0109431222305913E-5
5	3.0109431222305913E-5
6	3.0109431222305913E-5
7	2.258207341672943E-5
8	0.0
9	3.161490278342121E-4
10-11	7.038079548214006E-4
12-13	0.0
14-15	7.527357805576478E-6
16-17	0.001464071093184625
18-19	0.0
20-21	0.0
22-23	7.189348351819306E-4
24-25	0.002620246835534297
26-27	0.006910406039642157
28-29	0.3595760815803188
30-31	0.3306218489879632
32-33	0.3294347201749065
34-35	0.1843518550061993
36-37	0.04690884628398347
38-39	0.045693334384440676
40-41	0.0250958351533778
42-43	0.02511822202656558
44-45	0.01377099334218375
46-47	0.009547156284818024
48-49	0.009461630063644595
50-51	0.00825405598931726
52-53	0.007153440861667334
54-55	0.0010140552950913127
56-57	0.004247659303742566
58-59	0.002970150279262382
60-61	0.006142715421339193
62-63	0.0018204621543786383
64-65	1.8654109398550815E-4
66-67	8.001265141217623E-4
68-69	0.0018880050932069663
70-71	0.0044198977952083765
72-73	0.0023834489078429126
74-75	0.0016413922653948916
76-77	6.873818085168134E-4
78-79	1.6797115181722995E-4
80-81	0.0012575364988494917
82-83	0.0013782758298403865
84-85	0.0
86-87	0.0035654010632301327
88-89	0.0016200813165444194
90-91	0.004429051635774478
92-93	0.013183224176698507
94-95	0.001350682320522438
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	187.0
20-21	788.0
22-23	1746.0
24-25	3913.0
26-27	5651.0
28-29	9331.0
30-31	24210.0
32-33	32911.0
34-35	49283.0
36-37	65675.0
38-39	76711.0
40-41	83787.0
42-43	102073.0
44-45	123605.0
46-47	121763.0
48-49	105115.0
50-51	92332.0
52-53	85605.0
54-55	92536.0
56-57	96533.0
58-59	96547.0
60-61	98387.0
62-63	96644.0
64-65	106075.0
66-67	112128.0
68-69	113325.0
70-71	111969.0
72-73	108066.0
74-75	111103.0
76-77	113475.0
78-79	119041.0
80-81	120284.0
82-83	118843.0
84-85	121969.0
86-87	129055.0
88-89	136697.0
90-91	145890.0
92-93	151737.0
94-95	169528.0
96-97	254515.0
98-99	1130278.0
100-101	8445563.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.66149181296613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.06639844013806	74.5142776390999
2	7.486194557129988	12.526124093032042
3	1.865241835203368	4.6814674357520545
4	0.7098005922028303	2.3753190573366236
5	0.32827347135933754	1.3731924168271594
6	0.1810789868987958	0.9089602907960284
7	0.10452214639925753	0.6121135086678591
8	0.06485338550109945	0.4340584784114694
9	0.04161122581470028	0.31331315050416286
>10	0.1475871741076236	1.917038780319201
>50	0.0035667083855722933	0.20585796548056673
>100	8.233926555961262E-4	0.10478095551206225
>500	3.2056859155593314E-5	0.01932321585353634
>1k	1.6027344631985182E-5	0.014173012407300224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.269150463903534E-5	0.0	7.527357805576477E-6	0.0
3	0.0	5.269150463903534E-5	0.0	7.527357805576477E-6	0.0
4	0.0	9.78556514724942E-5	0.0	1.5054715611152955E-5	7.527357805576477E-6
5	0.0	1.0538300927807068E-4	0.0	2.2582073416729434E-5	7.527357805576477E-6
6	7.527357805576477E-6	1.0538300927807068E-4	0.0	3.010943122230591E-5	2.2582073416729434E-5
7	7.527357805576477E-6	1.0538300927807068E-4	0.0	3.010943122230591E-5	2.2582073416729434E-5
8	7.527357805576477E-6	1.279650826948001E-4	0.0	3.763678902788239E-5	3.010943122230591E-5
9	1.5054715611152955E-5	2.709848810007532E-4	0.0	3.763678902788239E-5	3.763678902788239E-5
10-11	1.5054715611152955E-5	2.709848810007532E-4	0.0	4.516414683345887E-5	3.763678902788239E-5
12-13	1.5054715611152955E-5	2.709848810007532E-4	0.0	4.892782573624711E-5	3.763678902788239E-5
14-15	1.5054715611152955E-5	3.236763856397885E-4	0.0	7.150989915297653E-5	4.516414683345887E-5
16-17	3.763678902788239E-5	3.236763856397885E-4	0.0	9.032829366691772E-5	5.269150463903534E-5
18-19	3.763678902788239E-5	3.688405324732474E-4	0.0	1.2043772488922364E-4	5.269150463903534E-5
20-21	3.763678902788239E-5	4.25295716015071E-4	0.0	1.543108350143178E-4	6.021886244461182E-5
22-23	3.763678902788239E-5	4.892782573624711E-4	0.0	5.419697620015064E-4	7.903725695855301E-5
24-25	4.516414683345886E-5	5.494971198070828E-4	0.0	0.0016823644695463428	9.78556514724942E-5
26-27	5.269150463903534E-5	5.796065510293888E-4	0.0	0.004471250536512428	9.78556514724942E-5
28-29	5.269150463903534E-5	5.796065510293888E-4	0.0	0.01786618375153577	9.78556514724942E-5
30-31	5.269150463903534E-5	6.699348446963065E-4	0.0	0.024565532198498836	9.78556514724942E-5
32-33	5.269150463903534E-5	6.77462202501883E-4	0.0	0.026597918806004484	1.2043772488922364E-4
34-35	5.645518354182358E-5	7.790815328771654E-4	0.0	0.029435732698706814	1.354924405003766E-4
36-37	6.021886244461182E-5	8.882282210580243E-4	0.0	0.03370750825337146	1.4301979830595306E-4
38-39	6.021886244461182E-5	8.995192577663891E-4	0.0	0.0410241000403918	1.4301979830595306E-4
40-41	6.021886244461182E-5	0.0010161933037528244	0.0	0.05600730575239178	1.5054715611152955E-4
42-43	6.021886244461182E-5	0.0010500664138779186	0.0	0.06345938997991249	1.5807451391710605E-4
44-45	6.398254134740007E-5	0.0011893225332810834	0.0	0.07364766876976026	1.6936555062547074E-4
46-47	6.77462202501883E-5	0.001294705542559154	0.0	0.08478815832201345	1.7312922952825898E-4
48-49	6.77462202501883E-5	0.0013022329003647306	0.0	0.0952963498185982	1.957113029449884E-4
50-51	6.77462202501883E-5	0.001433961661962319	0.0	0.10550344700295991	1.957113029449884E-4
52-53	6.77462202501883E-5	0.0014452526986706838	0.0	0.11849943025428769	2.1452969745892962E-4
54-55	6.77462202501883E-5	0.001467834772087413	0.0	0.1449016377573472	2.333480919728708E-4
56-57	6.77462202501883E-5	0.0015054715611152955	0.0	0.17312546584935617	2.3711177087565904E-4
58-59	6.77462202501883E-5	0.001512998918920872	0.0	0.20186491795104716	2.4087544977844728E-4
60-61	6.77462202501883E-5	0.001546872029045966	0.0	0.22059674785022426	2.4463912868123553E-4
62-63	6.77462202501883E-5	0.0015807451391710602	0.0	0.2315565808151436	2.4840280758402374E-4
64-65	6.77462202501883E-5	0.001893130488102484	0.0	0.24192551619232522	2.4840280758402374E-4
66-67	6.77462202501883E-5	0.0019119488826164252	0.0	0.24800009394142541	2.4840280758402374E-4
68-69	6.77462202501883E-5	0.0019608767083526724	0.0	0.2533708637357043	2.4840280758402374E-4
70-71	6.77462202501883E-5	0.0019909861395749785	0.0	0.25866259627302446	2.559301653896002E-4
72-73	7.527357805576478E-5	0.0019985134973805547	0.0	0.2625015487538685	2.860395966119061E-4
74-75	7.527357805576478E-5	0.002062496038727955	0.0	0.26553131027061305	2.860395966119061E-4
76-77	7.527357805576478E-5	0.002164115369103237	0.0	0.2684519250991767	2.860395966119061E-4
78-79	7.527357805576478E-5	0.002190461121422755	0.0	0.27086820695476677	2.860395966119061E-4
80-81	7.527357805576478E-5	0.0021942248003255435	0.0	0.27208011156146455	2.898032755146944E-4
82-83	7.527357805576478E-5	0.0021979884792283316	0.0	0.2725468077454103	3.086216700286356E-4
84-85	8.280093586134126E-5	0.002235625268256214	0.0	0.27282531998421666	3.086216700286356E-4
86-87	8.656461476412949E-5	0.0022619710205757314	0.0	0.2729269393145919	3.123853489314238E-4
88-89	9.032829366691773E-5	0.0023748813876593787	0.0	0.2729570487458142	3.1614902783421204E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18715	0.0	51.857216	1
GTATCAA	35040	0.0	38.25484	1
TCAACGC	43775	0.0	30.072289	4
ATCAACG	44215	0.0	29.801886	3
CAACGCA	45020	0.0	29.269331	5
TATCAAC	45460	0.0	29.137457	2
AACGCAG	46485	0.0	28.374557	6
GTGGTAT	8780	0.0	25.485819	1
ACGCAGA	53345	0.0	24.693533	7
TGGTATC	8600	0.0	24.424194	2
CGCAGAG	54655	0.0	24.117353	8
GCAGAGT	61820	0.0	21.148779	9
GTACATG	41425	0.0	20.458168	1
GAGTACT	37255	0.0	19.871113	12-13
TACATGG	41620	0.0	19.847336	2
ACATGGG	41635	0.0	19.077715	3
AGAGTAC	56830	0.0	19.026752	10-11
CAGAGTA	60845	0.0	18.535618	10-11
GTACTTT	41000	0.0	18.051134	14-15
AGTACTT	40620	0.0	17.818682	12-13
>>END_MODULE