FastQCFastQC Report
Fri 27 May 2016
SRR2139618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979594
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78500.051.3960461
CTTATAC105400.049.5115471
TATACAC132950.041.55523
TTATACA131250.040.3678862
GTATCAA142950.036.182831
TACACAT175500.031.407325
TCAACGC168400.030.2136944
ATCAACG169650.029.8436053
CAACGCA173000.029.3375265
ACACATC185550.028.801216
TATCAAC178900.028.5364022
ACATCTC189600.028.3409848
ATACACA201800.028.1878134
AACGCAG180750.028.1260746
CACATCT200700.026.4597917
CATCTCC214850.024.7562319
ACGCAGA205550.024.732617
CGCAGAG212050.023.9348858
TGGTATC34200.021.8509272
GTGGTAT38000.021.433551
Produced by FastQC (version 0.11.5)