##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139618_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5979594
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.306962981098717	32.0	25.0	33.0	18.0	33.0
2	29.569877486665483	31.0	28.0	33.0	18.0	33.0
3	31.993693050063264	33.0	31.0	33.0	29.0	34.0
4	31.860833026456312	33.0	31.0	33.0	29.0	34.0
5	32.84681351275689	33.0	33.0	34.0	32.0	34.0
6	36.81676214137615	38.0	37.0	38.0	35.0	38.0
7	36.98576374917762	38.0	38.0	38.0	35.0	38.0
8	37.12255531061139	38.0	38.0	38.0	36.0	38.0
9	37.206858860317276	38.0	38.0	38.0	36.0	38.0
10-11	37.31588357002164	38.0	38.0	38.0	36.5	38.0
12-13	37.3743026700475	38.0	38.0	38.0	37.0	38.0
14-15	37.38203965018361	38.0	38.0	38.0	37.0	38.0
16-17	37.43235025655588	38.0	38.0	38.0	37.0	38.0
18-19	37.42262551604674	38.0	38.0	38.0	37.0	38.0
20-21	37.441595692243766	38.0	38.0	38.0	37.0	38.0
22-23	37.45190815861055	38.0	38.0	38.0	37.0	38.0
24-25	37.46184018222453	38.0	38.0	38.0	37.0	38.0
26-27	37.41999373581778	38.0	38.0	38.0	37.0	38.0
28-29	37.38627520307109	38.0	38.0	38.0	37.0	38.0
30-31	37.39947117451082	38.0	38.0	38.0	37.0	38.0
32-33	37.38415289479826	38.0	38.0	38.0	37.0	38.0
34-35	37.345102139941375	38.0	38.0	38.0	37.0	38.0
36-37	37.3222908778084	38.0	38.0	38.0	37.0	38.0
38-39	37.28895096197573	38.0	38.0	38.0	37.0	38.0
40-41	37.35748416216329	38.0	38.0	38.0	37.0	38.0
42-43	37.333841604389605	38.0	38.0	38.0	37.0	38.0
44-45	37.30129742246875	38.0	38.0	38.0	37.0	38.0
46-47	37.28499886857658	38.0	38.0	38.0	37.0	38.0
48-49	37.233319561817844	38.0	38.0	38.0	37.0	38.0
50-51	37.191977653172245	38.0	38.0	38.0	37.0	38.0
52-53	37.17798633144096	38.0	38.0	38.0	37.0	38.0
54-55	37.157373583379	38.0	38.0	38.0	36.5	38.0
56-57	37.11120377217723	38.0	38.0	38.0	36.0	38.0
58-59	37.08096187183444	38.0	38.0	38.0	36.0	38.0
60-61	37.09718227379974	38.0	38.0	38.0	36.0	38.0
62-63	37.10491379413473	38.0	38.0	38.0	36.0	38.0
64-65	37.112659908859726	38.0	38.0	38.0	36.0	38.0
66-67	37.10780356303624	38.0	38.0	38.0	36.0	38.0
68-69	37.092619356431904	38.0	38.0	38.0	36.0	38.0
70-71	37.092133614451484	38.0	38.0	38.0	36.0	38.0
72-73	37.085489617665516	38.0	38.0	38.0	36.0	38.0
74-75	37.07460532704376	38.0	38.0	38.0	36.0	38.0
76-77	37.06464732676988	38.0	38.0	38.0	36.0	38.0
78-79	37.07768187438548	38.0	38.0	38.0	36.0	38.0
80-81	37.085895242137454	38.0	38.0	38.0	36.0	38.0
82-83	37.04690614983938	38.0	38.0	38.0	36.0	38.0
84-85	37.03941572507955	38.0	38.0	38.0	36.0	38.0
86-87	37.04546897615952	38.0	38.0	38.0	36.0	38.0
88-89	37.036740850184074	38.0	38.0	38.0	36.0	38.0
90-91	37.01829351146755	38.0	38.0	38.0	36.0	38.0
92-93	37.01524585612764	38.0	38.0	38.0	36.0	38.0
94-95	37.00218050654305	38.0	38.0	38.0	36.0	38.0
96-97	36.978222921083486	38.0	38.0	38.0	36.0	38.0
98-99	36.948209742889375	38.0	38.0	38.0	35.5	38.0
100	35.66068040576435	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	7.0
19	18.0
20	46.0
21	104.0
22	326.0
23	992.0
24	2304.0
25	4774.0
26	8859.0
27	14680.0
28	22937.0
29	33844.0
30	45623.0
31	59606.0
32	77436.0
33	103895.0
34	158415.0
35	297074.0
36	896922.0
37	4251726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.384832147466874	21.364276571285608	13.807158144850638	24.443733136396887
2	14.889184352683424	20.269947987222583	42.42006589400097	22.42080176609302
3	18.011294155109162	26.41354214887975	30.707351402554377	24.86781229345671
4	12.765314835756408	18.4030220111934	37.71655734486321	31.11510580818698
5	13.470379427098228	37.27291518454263	35.87631534850025	13.380390039858895
6	29.93131954506473	37.771474097829014	18.338373824498593	13.958832532607662
7	26.35946520783852	32.52538550276156	22.432258778773274	18.682890510626642
8	24.05701791793891	37.24426775463351	21.137371533920195	17.561342793507386
9	25.055848273310865	17.026373362472434	20.912774345549213	37.005004018667485
10-11	24.138118741841	26.66752960150806	28.868506791598225	20.325844865052712
12-13	25.15879840671457	23.98059968619943	28.729935510671794	22.130666396414206
14-15	22.263927952299102	25.55443563559666	25.898806842069877	26.282829570034355
16-17	22.085588085077347	29.112285884292476	26.62056152976272	22.181564500867452
18-19	22.191623712245345	27.755596784664643	28.355938546998345	21.696840956091666
20-21	22.702801327597758	26.87595262631361	28.300715341460403	22.12053070462823
22-23	22.446330465834908	27.455676532195866	28.14327072769201	21.954722274277216
24-25	22.462389627163247	27.753327837776652	28.25608179074306	21.528200744317036
26-27	22.41528392979587	27.98361609243587	28.252278920878922	21.34882105688934
28-29	22.19422211016599	27.56287703396779	28.400099444794456	21.84280141107176
30-31	22.8413096877709	27.3215814866818	28.35604202889723	21.481066796650065
32-33	22.255834837738462	27.598801404242128	28.522403945587715	21.622959812431695
34-35	22.337021564121624	26.98506730980447	28.757790069070666	21.920121057003236
36-37	21.993189675780247	27.609564000664804	28.57290857159218	21.824337751962766
38-39	21.938285225269908	26.831248612197683	29.100338992709084	22.13012716982333
40-41	22.13737558687181	26.732997420286104	29.160915422488838	21.968711570353253
42-43	22.52329650576648	26.612994804072542	28.955818690936486	21.90788999922449
44-45	21.926472522574144	27.219610190186405	28.87983204712079	21.97408524011866
46-47	22.674120230081876	26.6129629192299	28.255886251941696	22.457030598746528
48-49	22.40617803092431	26.7093973000734	28.257365061485363	22.62705960751693
50-51	22.01134100375859	26.93051910352544	28.70406382528793	22.35407606742804
52-53	22.18948493236878	27.00257470532823	29.097460913839583	21.710479448463403
54-55	22.14199153112498	27.091900767150754	28.44095759522793	22.325150106496334
56-57	22.605408941761944	27.638960993614862	27.68819333479738	22.06743672982581
58-59	21.80311269343083	28.1579123515934	28.576522280713494	21.462452674262273
60-61	22.364157193484665	28.927300262607652	27.36283989986511	21.345702644042575
62-63	21.750035103211943	29.49164600211951	27.407662500015345	21.350656394653207
64-65	21.722343187625352	29.329063674356554	27.45951856532742	21.489074572690665
66-67	21.868150808343493	29.204861441873252	27.389752590024575	21.537235159758676
68-69	21.989254131152766	28.74707137139706	27.607074935448132	21.65659956200204
70-71	22.158015572288363	28.103260960437087	27.878829076658366	21.85989439061618
72-73	22.413277283375827	27.725664744484256	27.826321402114235	22.03473657002568
74-75	22.229177796864693	27.736888204787665	27.92444322926464	22.109490769083003
76-77	22.4008979413053	27.6134616815015	27.77255390540418	22.213086471789023
78-79	22.369303968660446	27.56792068795389	27.78098841758645	22.28178692579922
80-81	22.381658470168205	27.65469635674093	27.699033312965625	22.26461186012524
82-83	22.453413280295152	27.539173293061182	27.81100604786279	22.196407378780876
84-85	22.451712207774303	27.558402054304647	27.758102180975282	22.23178355694577
86-87	22.402755055379306	27.58343289361435	27.792359884929674	22.221452166076666
88-89	22.47929677192365	27.574918638402675	27.74931832175213	22.19646626792154
90-91	22.519454625734397	27.566763348779055	27.764344354323416	22.149437671163135
92-93	22.566050366294913	27.55464302304318	27.719085447907517	22.160221162754393
94-95	22.374839787358315	27.576782444842923	27.807709141926267	22.240668625872495
96-97	22.53067730960246	27.663059232943443	27.829359914966727	21.97690354248737
98-99	23.54696054595256	29.618485240557916	28.017784017083592	18.81677019640593
100	30.03793851697571	40.609250842531615	29.35281064049267	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1273.0
1	1113.0
2	1184.5
3	1566.5
4	1977.0
5	2646.5
6	3347.0
7	4365.0
8	5647.5
9	6456.0
10	6597.0
11	6804.5
12	7662.5
13	9534.0
14	13232.5
15	17284.0
16	20146.0
17	21765.0
18	21760.5
19	20315.0
20	18570.5
21	17497.0
22	17828.0
23	20134.0
24	24867.0
25	32172.5
26	41896.5
27	53265.0
28	65146.5
29	78485.0
30	92283.5
31	105847.0
32	121887.0
33	139004.5
34	153817.5
35	169181.5
36	187145.5
37	201342.0
38	213322.5
39	223555.0
40	231707.5
41	238748.5
42	245974.5
43	271373.0
44	297403.5
45	302410.0
46	290951.0
47	272145.0
48	258256.0
49	249438.0
50	243249.5
51	235847.5
52	226435.5
53	214080.0
54	198909.0
55	183778.5
56	167223.5
57	149326.5
58	132722.0
59	114832.5
60	96085.0
61	77996.5
62	61894.5
63	48078.5
64	35915.5
65	26873.5
66	20302.0
67	15600.5
68	12024.0
69	9108.0
70	6786.0
71	4948.5
72	3569.0
73	2479.0
74	1706.5
75	1109.0
76	733.5
77	509.5
78	354.5
79	232.0
80	142.5
81	85.5
82	47.0
83	26.0
84	22.0
85	17.5
86	12.5
87	9.5
88	6.0
89	4.0
90	4.0
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004916721770742294
3	0.0036457324694619737
4	0.0
5	0.0
6	3.010237818821813E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.843386362767762E-4
26-27	0.0018066100852000663
28-29	0.0070118558098036625
30-31	0.002404456370845925
32-33	0.00497290021388511
34-35	0.0026214169087122327
36-37	0.0010334481420804836
38-39	3.412190477002902E-5
40-41	8.593023822354012E-5
42-43	0.002129149352903043
44-45	6.022470623435685E-4
46-47	3.789096759520392E-4
48-49	0.0018945308452302605
50-51	4.1270510883168295E-4
52-53	0.0
54-55	0.00497362022802318
56-57	0.05000672725945226
58-59	0.07514920062261186
60-61	0.03608745767319853
62-63	0.015266503874312016
64-65	0.0016789868687963343
66-67	0.0
68-69	0.0024896766635352937
70-71	0.004150075900608768
72-73	0.015336094302706766
74-75	0.018643600826904548
76-77	0.02429181911022537
78-79	0.006700319246207823
80-81	0.008894033759768665
82-83	0.036573745893717334
84-85	0.021118082387659286
86-87	0.015457968142008796
88-89	0.007475912171224773
90-91	6.669771815989889E-5
92-93	0.0
94-95	0.0
96-97	8.08124840972576E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	199.0
22-23	469.0
24-25	587.0
26-27	1761.0
28-29	4833.0
30-31	15124.0
32-33	18874.0
34-35	25869.0
36-37	39635.0
38-39	43437.0
40-41	41097.0
42-43	45992.0
44-45	53758.0
46-47	53768.0
48-49	49797.0
50-51	43750.0
52-53	40697.0
54-55	44666.0
56-57	45904.0
58-59	48123.0
60-61	52445.0
62-63	53632.0
64-65	62850.0
66-67	71935.0
68-69	71474.0
70-71	95195.0
72-73	52974.0
74-75	47305.0
76-77	49650.0
78-79	51618.0
80-81	50951.0
82-83	46733.0
84-85	46226.0
86-87	47922.0
88-89	49756.0
90-91	49773.0
92-93	48457.0
94-95	56225.0
96-97	173760.0
98-99	1336151.0
100-101	2846184.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.17638437616839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.35355766260976	80.55226414588742
2	6.426350540545513	11.333047107982773
3	1.311107463021598	3.4682614685356628
4	0.4420732363440585	1.5592167844116176
5	0.19666585317725543	0.8670641931712387
6	0.09427830202795597	0.4987871878769724
7	0.054625579635421403	0.337168027469274
8	0.035198889111355186	0.24829686207175872
9	0.02408516842652193	0.1911368762047577
>10	0.06056032857593112	0.799216097101593
>50	0.001085361666994493	0.06410404955709356
>100	3.847372846254452E-4	0.06600871321109754
>500	2.6877572991432287E-5	0.015428486518813771
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6723543437898962E-5
2	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
3	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
4	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
5	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
6	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
7	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
8	1.6723543437898962E-5	0.0	0.0	1.6723543437898962E-5	1.6723543437898962E-5
9	8.36177171894948E-5	3.3447086875797924E-5	0.0	5.017063031369688E-5	3.3447086875797924E-5
10-11	1.0870303234634325E-4	3.3447086875797924E-5	0.0	1.0034126062739377E-4	3.3447086875797924E-5
12-13	1.1706480406529273E-4	3.3447086875797924E-5	0.0	1.337883475031917E-4	3.3447086875797924E-5
14-15	1.1706480406529273E-4	7.525594547054532E-5	0.0	1.5051189094109066E-4	5.017063031369688E-5
16-17	1.254265757842422E-4	1.0034126062739377E-4	0.0	1.9232074953583805E-4	6.689417375159585E-5
18-19	1.5051189094109063E-4	1.1706480406529273E-4	0.0	2.0068252125478753E-4	9.197948890844428E-5
20-21	1.7559720609793908E-4	1.337883475031917E-4	0.0	2.0068252125478753E-4	1.0034126062739377E-4
22-23	2.09044292973737E-4	1.337883475031917E-4	0.0	2.2576783641163598E-4	1.254265757842422E-4
24-25	2.4249137984953494E-4	1.672354343789896E-4	0.0	2.508531515684844E-4	1.337883475031917E-4
26-27	2.675766950063834E-4	2.0068252125478753E-4	0.0	3.0102378188218127E-4	1.337883475031917E-4
28-29	2.675766950063834E-4	2.0068252125478753E-4	0.0	8.779860304896955E-4	1.337883475031917E-4
30-31	2.675766950063834E-4	2.508531515684844E-4	0.0	0.0025837874611553896	1.337883475031917E-4
32-33	2.7593846672533285E-4	2.675766950063834E-4	0.0	0.0060288374093625755	1.337883475031917E-4
34-35	2.9266201016323187E-4	3.428326404769287E-4	0.0	0.0104605764204058	1.337883475031917E-4
36-37	3.010237818821813E-4	4.0972681422852457E-4	0.0	0.017735317815891848	1.337883475031917E-4
38-39	3.010237818821813E-4	4.264503576664235E-4	0.0	0.034007325580967536	1.4215011922214118E-4
40-41	3.010237818821813E-4	5.518769334506657E-4	0.0	0.051056978115905524	1.5051189094109066E-4
42-43	3.010237818821813E-4	5.686004768885647E-4	0.0	0.0612081689827102	1.5051189094109066E-4
44-45	3.1774732532008023E-4	6.4385642235911E-4	0.0	0.07399331794098396	1.5051189094109066E-4
46-47	3.5119441219587816E-4	7.441976829865038E-4	0.0	0.08697078764879354	1.5887366266004014E-4
48-49	3.846414990716761E-4	7.525594547054533E-4	0.0	0.09875252400079337	1.672354343789896E-4
50-51	4.0136504250957506E-4	8.445389436138975E-4	0.0	0.11151258764391027	1.672354343789896E-4
52-53	4.0136504250957506E-4	8.52900715332847E-4	0.0	0.1445248623903228	1.7559720609793908E-4
54-55	4.0136504250957506E-4	8.52900715332847E-4	0.0	0.18452757829377714	1.8395897781688856E-4
56-57	4.0136504250957506E-4	8.52900715332847E-4	0.0	0.24113677283106513	1.8395897781688856E-4
58-59	4.0136504250957506E-4	8.69624258770746E-4	0.0	0.2784469982410177	1.8395897781688856E-4
60-61	4.0972681422852457E-4	8.69624258770746E-4	0.0	0.29195962133884007	1.9232074953583805E-4
62-63	4.18088585947474E-4	8.69624258770746E-4	0.0	0.30165927653282143	2.0068252125478753E-4
64-65	4.264503576664235E-4	0.0010034126062739377	0.0	0.307362004845145	2.0068252125478753E-4
66-67	4.431739011043225E-4	0.0010117743779928872	0.0	0.3124125149633905	2.0068252125478753E-4
68-69	4.5153567282327196E-4	0.001078668551744483	0.0	0.31728742787553804	2.0068252125478753E-4
70-71	4.5153567282327196E-4	0.0011204774103392304	0.0	0.32168571979970545	2.174060646926865E-4
72-73	4.5153567282327196E-4	0.0011372009537771294	0.0	0.3259418616046508	2.508531515684844E-4
74-75	4.598974445422214E-4	0.0011706480406529272	0.0	0.3291109730861326	2.508531515684844E-4
76-77	4.682592162611709E-4	0.0011957333558097758	0.0	0.33208776381807864	2.5921492328743394E-4
78-79	4.849827596990699E-4	0.0012040951275287253	0.0	0.33427854800844337	2.675766950063834E-4
80-81	4.849827596990699E-4	0.0012040951275287253	0.0	0.3351481722672141	2.675766950063834E-4
82-83	4.849827596990699E-4	0.0012124568992476748	0.0	0.33573349628754057	2.8430023844428236E-4
84-85	4.849827596990699E-4	0.001220818670966624	0.0	0.3359592641239522	2.8430023844428236E-4
86-87	4.849827596990699E-4	0.0012626275295613714	0.0	0.33605124361286065	2.8430023844428236E-4
88	4.849827596990699E-4	0.0013713305619077148	0.0	0.33605960538457963	2.8430023844428236E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7850	0.0	51.396046	1
CTTATAC	10540	0.0	49.511547	1
TATACAC	13295	0.0	41.5552	3
TTATACA	13125	0.0	40.367886	2
GTATCAA	14295	0.0	36.18283	1
TACACAT	17550	0.0	31.40732	5
TCAACGC	16840	0.0	30.213694	4
ATCAACG	16965	0.0	29.843605	3
CAACGCA	17300	0.0	29.337526	5
ACACATC	18555	0.0	28.80121	6
TATCAAC	17890	0.0	28.536402	2
ACATCTC	18960	0.0	28.340984	8
ATACACA	20180	0.0	28.187813	4
AACGCAG	18075	0.0	28.126074	6
CACATCT	20070	0.0	26.459791	7
CATCTCC	21485	0.0	24.756231	9
ACGCAGA	20555	0.0	24.73261	7
CGCAGAG	21205	0.0	23.934885	8
TGGTATC	3420	0.0	21.850927	2
GTGGTAT	3800	0.0	21.43355	1
>>END_MODULE