FastQCFastQC Report
Fri 27 May 2016
SRR2139617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5841147
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.050.1302261
GTATCAA123700.036.8858531
TCAACGC141600.031.3393764
ATCAACG143650.030.9230733
CAACGCA146050.030.355115
TATCAAC152000.029.4535062
AACGCAG151800.029.2335666
ACGCAGA175850.025.259657
CGCAGAG179500.024.7221038
GTGGTAT37200.022.731051
TGGTATC34650.022.5457342
GCAGAGT211050.021.229739
GAGTACT118800.019.85091812-13
CGAGACC61100.019.62815386-87
AGACCGA62300.019.42141288-89
GAGACCG61150.019.2420788-89
GTACATG161400.019.1480791
ACCGAGG64900.018.94249390-91
CAGAGTA207650.018.89050510-11
GACCGAG63800.018.7829490-91
Produced by FastQC (version 0.11.5)