##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5841147
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.541380828114754	32.0	25.0	33.0	18.0	34.0
2	29.761985616866003	31.0	28.0	33.0	25.0	33.0
3	32.040300475232	33.0	31.0	33.0	29.0	34.0
4	31.91176904125166	33.0	31.0	33.0	29.0	34.0
5	32.85406205322345	33.0	33.0	34.0	32.0	34.0
6	36.82258227707675	38.0	37.0	38.0	35.0	38.0
7	36.99188892181621	38.0	38.0	38.0	35.0	38.0
8	37.12661365995412	38.0	38.0	38.0	36.0	38.0
9	37.20461306657751	38.0	38.0	38.0	36.0	38.0
10-11	37.32317685208059	38.0	38.0	38.0	36.5	38.0
12-13	37.38152506690895	38.0	38.0	38.0	37.0	38.0
14-15	37.387283696164474	38.0	38.0	38.0	37.0	38.0
16-17	37.435740617382166	38.0	38.0	38.0	37.0	38.0
18-19	37.426733139912415	38.0	38.0	38.0	37.0	38.0
20-21	37.442185348042145	38.0	38.0	38.0	37.0	38.0
22-23	37.450160092156096	38.0	38.0	38.0	37.0	38.0
24-25	37.46371657647509	38.0	38.0	38.0	37.0	38.0
26-27	37.42346386850893	38.0	38.0	38.0	37.0	38.0
28-29	37.39285805987038	38.0	38.0	38.0	37.0	38.0
30-31	37.40928715972557	38.0	38.0	38.0	37.0	38.0
32-33	37.40370967488863	38.0	38.0	38.0	37.0	38.0
34-35	37.37528095206934	38.0	38.0	38.0	37.0	38.0
36-37	37.356034463456666	38.0	38.0	38.0	37.0	38.0
38-39	37.35374618388218	38.0	38.0	38.0	37.0	38.0
40-41	37.3665796255607	38.0	38.0	38.0	37.0	38.0
42-43	37.33509589862406	38.0	38.0	38.0	37.0	38.0
44-45	37.304767111217444	38.0	38.0	38.0	37.0	38.0
46-47	37.28890825325152	38.0	38.0	38.0	37.0	38.0
48-49	37.240057440057726	38.0	38.0	38.0	37.0	38.0
50-51	37.19981663383598	38.0	38.0	38.0	37.0	38.0
52-53	37.18725980025391	38.0	38.0	38.0	37.0	38.0
54-55	37.17159570070241	38.0	38.0	38.0	37.0	38.0
56-57	37.12593873500924	38.0	38.0	38.0	36.0	38.0
58-59	37.10488974268297	38.0	38.0	38.0	36.0	38.0
60-61	37.13053854920068	38.0	38.0	38.0	36.0	38.0
62-63	37.14043883618889	38.0	38.0	38.0	36.0	38.0
64-65	37.14828461224438	38.0	38.0	38.0	36.0	38.0
66-67	37.146479222105896	38.0	38.0	38.0	36.0	38.0
68-69	37.13961749487241	38.0	38.0	38.0	36.0	38.0
70-71	37.134026859130756	38.0	38.0	38.0	36.0	38.0
72-73	37.12912168425392	38.0	38.0	38.0	36.0	38.0
74-75	37.11373484262765	38.0	38.0	38.0	36.0	38.0
76-77	37.09635318685184	38.0	38.0	38.0	36.0	38.0
78-79	37.100131259982504	38.0	38.0	38.0	36.0	38.0
80-81	37.09634512979689	38.0	38.0	38.0	36.0	38.0
82-83	37.05309489321127	38.0	38.0	38.0	36.0	38.0
84-85	37.04097243995764	38.0	38.0	38.0	36.0	38.0
86-87	37.042399533791354	38.0	38.0	38.0	36.0	38.0
88-89	37.027560029421274	38.0	38.0	38.0	36.0	38.0
90-91	37.00574158965738	38.0	38.0	38.0	36.0	38.0
92-93	36.99986100146448	38.0	38.0	38.0	36.0	38.0
94-95	36.97328995135618	38.0	38.0	38.0	36.0	38.0
96-97	36.94864445428152	38.0	38.0	38.0	35.0	38.0
98-99	36.915647527542504	38.0	38.0	38.0	35.0	38.0
100	35.56064826982493	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	22.0
21	64.0
22	254.0
23	921.0
24	2317.0
25	4661.0
26	8344.0
27	13950.0
28	21765.0
29	32297.0
30	43718.0
31	57336.0
32	74696.0
33	100104.0
34	150599.0
35	275896.0
36	821048.0
37	4233148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.142604354932345	21.07925035956123	13.917540510451115	23.86060477505531
2	15.140501810524153	20.660828475307532	41.78768501159934	22.410984702568975
3	18.401237339958776	26.084294640721655	30.21412562445246	25.30034239486711
4	13.02519864677263	17.828553193405337	38.49589815151031	30.650350008311722
5	13.682437261872456	37.35262521303614	35.663161931436385	13.30177559365501
6	30.52978808202799	37.834421707587985	18.31252456717264	13.323265643211379
7	26.770803747962514	32.6982868262004	22.912366355443545	17.61854307039354
8	24.14767168160637	37.52701310204999	20.852599669208804	17.472715547134836
9	25.389054581232077	17.361915390932637	21.4096991566896	35.839330871145684
10-11	24.150881667590287	27.01052550124145	28.91504870533133	19.92354412583693
12-13	24.960714051538165	24.512197689940006	29.048036284654366	21.47905197386746
14-15	22.166879210538614	26.078482530913877	26.508586412908286	25.246051845639222
16-17	21.80611958575944	29.218927378475495	27.31875263539849	21.656200400366572
18-19	21.836884091429305	28.304218332461073	28.933401265196718	20.92549631091291
20-21	22.48982232870529	27.453428898739972	28.938449987677828	21.118298784876913
22-23	22.294774498377613	27.321446227030062	28.803104304157433	21.58067497043489
24-25	22.127465953490603	27.463256822143155	28.868340941077363	21.540936283288875
26-27	22.002956740609157	27.66591896249861	28.81811798588549	21.513006311006748
28-29	21.940241735816613	27.542627180928775	28.89941161270297	21.617719470551645
30-31	22.213694039732033	27.51627082059357	28.819976830738973	21.450058308935425
32-33	22.07395029220701	27.627648833401242	28.784123497955292	21.514277376436457
34-35	22.08329080198827	27.60121060359193	28.876401542412687	21.439097052007114
36-37	22.14669135420243	27.537893954868853	28.75750549805028	21.557909192878437
38-39	21.936479697708567	27.673384640592335	28.874370037748164	21.515765623950934
40-41	22.125740561698397	27.52014051382936	28.81340941991331	21.54070950455893
42-43	22.098212026632368	27.630012325236635	28.612687029195545	21.659088618935453
44-45	22.014825225503756	27.777168145797337	28.56211905633933	21.645887572359573
46-47	22.19933323639525	27.68068130967937	28.273311411742302	21.846674042183082
48-49	22.142187183241354	27.757549273566845	28.270850320982095	21.82941322220971
50-51	22.057908156502407	27.92582234506662	28.235650974227006	21.780618524203966
52-53	22.237943028662823	27.884087217506536	28.17285724458547	21.70511250924517
54-55	22.208759279297357	27.830099971786424	28.11983911358748	21.841301635328733
56-57	22.203042015972372	27.945458434303156	28.043363783186653	21.808135766537827
58-59	22.056594508503334	27.935971576294477	28.115674335491097	21.89175957971109
60-61	22.317441498890993	28.02885387710469	28.114727840318203	21.53897678368611
62-63	21.989389882748974	28.16546290838417	28.147197030492844	21.697950178374015
64-65	21.966083004875816	28.122209391821507	28.124920353566687	21.78678724973599
66-67	22.034108727226496	28.275888306294718	28.111441361960495	21.578561604518292
68-69	22.02783935473657	28.322510742526713	28.02844537833104	21.621204524405673
70-71	22.108942126539514	27.98713335953232	27.962440442087694	21.941484071840478
72-73	22.211023865794346	28.105450418003624	27.752265633216457	21.931260082985577
74-75	21.98200808468819	28.197542636037653	28.00342481936801	21.817024459906147
76-77	22.191066846807754	27.709839723413566	28.027963437247955	22.07112999253073
78-79	22.122650400445682	27.701774790929278	27.772547629303812	22.403027179321228
80-81	22.335964120937184	28.01295976493028	27.690708044436423	21.96036806969611
82-83	22.415659673050207	27.668929353241083	27.798700930057414	22.116710043651292
84-85	22.223481843367445	27.666769866796116	27.742203412502974	22.367544877333465
86-87	22.162607306847672	27.894428326883947	27.833824647549303	22.10913971871907
88-89	22.42942527472905	27.887251233319716	27.774786979851484	21.90853651209975
90-91	22.500138541240446	27.90756311543083	27.7276601300867	21.864638213242028
92-93	22.292096076763176	27.712695043547665	27.687851385368333	22.307357494320822
94-95	22.423147075255425	27.91281196771006	27.782304438553197	21.881736518481322
96-97	22.693803925932325	27.807499098148984	27.829202087947003	21.669494887971688
98-99	23.419975288927393	29.866282000570106	28.135639048188548	18.578103662313953
100	22.31380722597598	30.67262533752232	22.17169256497877	24.84187487152293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1191.0
1	986.0
2	976.0
3	1299.5
4	1700.0
5	2338.0
6	2917.0
7	3777.5
8	4847.5
9	5626.5
10	5955.0
11	6116.5
12	6836.5
13	8533.0
14	11739.0
15	15418.0
16	18100.5
17	19549.5
18	19642.0
19	18623.5
20	17350.0
21	16796.0
22	17571.0
23	20437.5
24	25819.0
25	33570.5
26	43752.0
27	55500.0
28	68103.0
29	81824.5
30	95592.0
31	109458.0
32	125222.5
33	141146.0
34	156611.0
35	170782.0
36	185743.0
37	199146.5
38	208572.0
39	217711.5
40	226446.5
41	232821.0
42	235160.5
43	239225.5
44	243631.5
45	247045.5
46	251767.5
47	252448.5
48	249298.0
49	244770.5
50	238470.5
51	229257.5
52	218270.5
53	205957.5
54	191211.0
55	176055.0
56	160313.0
57	143616.0
58	126505.0
59	108802.0
60	90729.5
61	73300.0
62	57662.5
63	44031.0
64	32836.0
65	24320.5
66	17926.5
67	13429.0
68	10182.0
69	7556.0
70	5608.5
71	4106.0
72	2921.0
73	2049.5
74	1378.5
75	884.5
76	569.0
77	352.5
78	232.5
79	159.5
80	98.5
81	61.0
82	44.0
83	30.0
84	18.5
85	11.5
86	9.5
87	7.0
88	5.5
89	7.0
90	6.5
91	4.5
92	3.5
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005084617798524845
3	0.003937582806938432
4	0.0
5	3.42398504951168E-5
6	3.252785797036096E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.653857154512101E-4
26-27	0.0017551432976043234
28-29	0.00724543411326806
30-31	0.0023060710019544165
32-33	0.004561432444412374
34-35	0.0026444106633663504
36-37	0.001011821272236394
38-39	1.7393860489063173E-5
40-41	5.2487955326451465E-5
42-43	0.0020592973800545077
44-45	4.8750623453995854E-4
46-47	3.2173568537610317E-4
48-49	0.0016668265919207563
50-51	4.5394599822089486E-4
52-53	0.0
54-55	0.0049704968794023995
56-57	0.0481917385670526
58-59	0.07300635854306448
60-61	0.03422138002316611
62-63	0.01442881354536115
64-65	0.0013650078798182154
66-67	0.0
68-69	0.0022657300741438288
70-71	0.0037519254400090202
72-73	0.013977801584461684
74-75	0.017137178307535476
76-77	0.022207841348313016
78-79	0.006153337496785569
80-81	0.008103062710282724
82-83	0.033613889909095675
84-85	0.01935679300943247
86-87	0.014535548866678438
88-89	0.007069417496089561
90-91	8.675764868986732E-5
92-93	0.0
94-95	0.0
96-97	5.6312863457298966E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	129.0
22-23	350.0
24-25	458.0
26-27	1301.0
28-29	3767.0
30-31	11387.0
32-33	14538.0
34-35	20141.0
36-37	31519.0
38-39	33633.0
40-41	33520.0
42-43	38257.0
44-45	45595.0
46-47	46003.0
48-49	43178.0
50-51	38695.0
52-53	36739.0
54-55	40560.0
56-57	42741.0
58-59	23344.0
60-61	43529.0
62-63	42562.0
64-65	45952.0
66-67	48937.0
68-69	49945.0
70-71	52618.0
72-73	45529.0
74-75	45604.0
76-77	48364.0
78-79	49720.0
80-81	49295.0
82-83	46280.0
84-85	45949.0
86-87	47818.0
88-89	49824.0
90-91	50480.0
92-93	49775.0
94-95	57711.0
96-97	197847.0
98-99	390372.0
100-101	3877151.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.34128182975633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.1634856982812	82.34003950182857
2	5.895428382755933	10.534102573018846
3	1.1783036515672067	3.1581347584709043
4	0.3692342797195937	1.3195145538254123
5	0.16307069117181544	0.7284472289077161
6	0.08673444892206286	0.4649380107296774
7	0.04502206394042414	0.28156302321411203
8	0.025359093804425453	0.18124911572227215
9	0.019543801419372214	0.15714614435696322
>10	0.052511171509967605	0.7067282924567211
>50	0.0010417824640337092	0.06579060286553022
>100	2.388596811507456E-4	0.04974331432775758
>500	2.6074762790946263E-5	0.01260288027552136
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.71199252475584E-5	0.0
3	0.0	0.0	0.0	1.71199252475584E-5	0.0
4	0.0	3.42398504951168E-5	0.0	1.71199252475584E-5	0.0
5	0.0	5.1359775742675196E-5	0.0	1.71199252475584E-5	0.0
6	6.84797009902336E-5	5.1359775742675196E-5	0.0	1.71199252475584E-5	0.0
7	6.84797009902336E-5	5.1359775742675196E-5	0.0	1.71199252475584E-5	0.0
8	8.5599626237792E-5	5.1359775742675196E-5	0.0	1.71199252475584E-5	0.0
9	1.0271955148535039E-4	5.1359775742675196E-5	0.0	1.71199252475584E-5	3.42398504951168E-5
10-11	1.0271955148535039E-4	5.1359775742675196E-5	0.0	6.84797009902336E-5	3.42398504951168E-5
12-13	1.0271955148535039E-4	5.1359775742675196E-5	0.0	6.84797009902336E-5	3.42398504951168E-5
14-15	1.0271955148535039E-4	1.0271955148535039E-4	0.0	7.70396636140128E-5	1.0271955148535039E-4
16-17	1.0271955148535039E-4	1.0271955148535039E-4	0.0	1.0271955148535039E-4	1.0271955148535039E-4
18-19	1.0271955148535039E-4	1.1127951410912959E-4	0.0	1.2839943935668798E-4	1.0271955148535039E-4
20-21	1.0271955148535039E-4	1.198394767329088E-4	0.0	1.369594019804672E-4	1.0271955148535039E-4
22-23	1.0271955148535039E-4	1.369594019804672E-4	0.0	1.455193646042464E-4	1.1127951410912959E-4
24-25	1.0271955148535039E-4	1.455193646042464E-4	0.0	1.7119925247558398E-4	1.198394767329088E-4
26-27	1.0271955148535039E-4	1.626392898518048E-4	0.0	2.482389160895968E-4	1.2839943935668798E-4
28-29	1.0271955148535039E-4	1.71199252475584E-4	0.0	5.478376079218688E-4	1.369594019804672E-4
30-31	1.0271955148535039E-4	1.71199252475584E-4	0.0	0.0016007130106467102	1.369594019804672E-4
32-33	1.1127951410912959E-4	1.71199252475584E-4	0.0	0.0038605431433244193	1.626392898518048E-4
34-35	1.455193646042464E-4	1.968791403469216E-4	0.0	0.007173248678726969	1.8831917772314238E-4
36-37	1.540793272280256E-4	2.0543910297070078E-4	0.0	0.012215066664132917	1.968791403469216E-4
38-39	1.626392898518048E-4	2.396789534658176E-4	0.0	0.0250207707493066	2.0543910297070078E-4
40-41	1.71199252475584E-4	2.396789534658176E-4	0.0	0.03826303292829302	2.0543910297070078E-4
42-43	1.71199252475584E-4	2.482389160895968E-4	0.0	0.04623235813103146	2.0543910297070078E-4
44-45	1.71199252475584E-4	3.3383854232738876E-4	0.0	0.056589912905804285	2.0543910297070078E-4
46-47	1.71199252475584E-4	3.7663835544628476E-4	0.0	0.06780346394295503	2.0543910297070078E-4
48-49	1.71199252475584E-4	3.7663835544628476E-4	0.0	0.07709102338975547	2.0543910297070078E-4
50-51	2.0543910297070078E-4	4.2799813118895994E-4	0.0	0.08798785580982639	2.2255902821825919E-4
52-53	2.0543910297070078E-4	4.4511805643651837E-4	0.0	0.11730572779627016	2.2255902821825919E-4
54-55	2.0543910297070078E-4	4.4511805643651837E-4	0.0	0.15693835474436785	2.311189908420384E-4
56-57	2.0543910297070078E-4	4.4511805643651837E-4	0.0	0.2048142256991649	2.396789534658176E-4
58-59	2.0543910297070078E-4	4.622379816840768E-4	0.0	0.4075569404433752	2.396789534658176E-4
60-61	2.1399906559447997E-4	4.793579069316352E-4	0.0	0.5907658204801215	2.396789534658176E-4
62-63	2.5679887871337596E-4	4.793579069316352E-4	0.0	0.597682270280135	2.5679887871337596E-4
64-65	2.5679887871337596E-4	5.991973836645439E-4	0.0	0.6035629646026714	2.5679887871337596E-4
66-67	2.6535884133715515E-4	5.991973836645439E-4	0.0	0.6093152594858511	2.5679887871337596E-4
68-69	2.739188039609344E-4	6.077573462883231E-4	0.0	0.614973394780169	2.5679887871337596E-4
70-71	2.824787665847136E-4	6.163173089121024E-4	0.0	0.6198953732888421	2.5679887871337596E-4
72-73	3.081586544560512E-4	6.163173089121024E-4	0.0	0.6246461525450395	2.739188039609344E-4
74-75	3.081586544560512E-4	6.334372341596608E-4	0.0	0.6277534189774714	2.739188039609344E-4
76-77	3.081586544560512E-4	6.676770846547775E-4	0.0	0.6310062047745075	2.739188039609344E-4
78-79	3.252785797036096E-4	6.84797009902336E-4	0.0	0.6330862756920859	2.739188039609344E-4
80-81	3.252785797036096E-4	6.84797009902336E-4	0.0	0.633813872515107	2.739188039609344E-4
82-83	3.252785797036096E-4	6.84797009902336E-4	0.0	0.6344216298613954	2.739188039609344E-4
84-85	3.252785797036096E-4	6.84797009902336E-4	0.0	0.6347554684037228	2.824787665847136E-4
86-87	3.252785797036096E-4	7.019169351498944E-4	0.0	0.6349437875814459	2.9103872920849277E-4
88	3.252785797036096E-4	7.532767108925695E-4	0.0	0.6349437875814459	2.9103872920849277E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6790	0.0	50.130226	1
GTATCAA	12370	0.0	36.885853	1
TCAACGC	14160	0.0	31.339376	4
ATCAACG	14365	0.0	30.923073	3
CAACGCA	14605	0.0	30.35511	5
TATCAAC	15200	0.0	29.453506	2
AACGCAG	15180	0.0	29.233566	6
ACGCAGA	17585	0.0	25.25965	7
CGCAGAG	17950	0.0	24.722103	8
GTGGTAT	3720	0.0	22.73105	1
TGGTATC	3465	0.0	22.545734	2
GCAGAGT	21105	0.0	21.22973	9
GAGTACT	11880	0.0	19.850918	12-13
CGAGACC	6110	0.0	19.628153	86-87
AGACCGA	6230	0.0	19.421412	88-89
GAGACCG	6115	0.0	19.24207	88-89
GTACATG	16140	0.0	19.148079	1
ACCGAGG	6490	0.0	18.942493	90-91
CAGAGTA	20765	0.0	18.890505	10-11
GACCGAG	6380	0.0	18.78294	90-91
>>END_MODULE