FastQCFastQC Report
Fri 27 May 2016
SRR2139616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6260350
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT69750.059.16303694
GGTATCA83900.051.495641
TATCTCG85250.044.1563394
GTATCAA149250.036.5734251
TCAACGC175150.030.4533794
ATCAACG179900.029.6970463
CAACGCA180750.029.509875
TATCAAC184550.029.321822
AACGCAG187050.028.6077946
CCGTCTT43400.028.19560690-91
TATGCCG42850.027.50398486-87
GCCGTCT46350.026.70522390-91
TCTCGTA43550.026.32247582-83
ATGCCGT46800.026.30023888-89
CTATCTC106450.026.27773392-93
TGCCGTC46050.026.18678388-89
TGGTATC39800.024.8187082
CTCGTAT46300.024.81689582-83
ACGCAGA216900.024.6113597
TCGTATG48700.024.53913984-85
Produced by FastQC (version 0.11.5)