##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139616_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6260350
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.225624286182082	32.0	25.0	33.0	18.0	33.0
2	29.579254674259428	31.0	28.0	33.0	25.0	33.0
3	32.00283945785779	33.0	31.0	33.0	29.0	34.0
4	31.893233445414396	33.0	31.0	33.0	29.0	34.0
5	32.86325636745549	33.0	33.0	34.0	32.0	34.0
6	36.842071928885765	38.0	37.0	38.0	35.0	38.0
7	37.01870917760189	38.0	38.0	38.0	35.0	38.0
8	37.13301764278355	38.0	38.0	38.0	36.0	38.0
9	37.21880981095306	38.0	38.0	38.0	36.0	38.0
10-11	37.33282020973268	38.0	38.0	38.0	36.5	38.0
12-13	37.38985288362472	38.0	38.0	38.0	37.0	38.0
14-15	37.395373341746065	38.0	38.0	38.0	37.0	38.0
16-17	37.44529874527782	38.0	38.0	38.0	37.0	38.0
18-19	37.43681335708067	38.0	38.0	38.0	37.0	38.0
20-21	37.451853194766606	38.0	38.0	38.0	37.0	38.0
22-23	37.46074097546297	38.0	38.0	38.0	37.0	38.0
24-25	37.474804727407744	38.0	38.0	38.0	37.0	38.0
26-27	37.43833041011917	38.0	38.0	38.0	37.0	38.0
28-29	37.408446530162436	38.0	38.0	38.0	37.0	38.0
30-31	37.424911311882255	38.0	38.0	38.0	37.0	38.0
32-33	37.4167783458481	38.0	38.0	38.0	37.0	38.0
34-35	37.38999139769633	38.0	38.0	38.0	37.0	38.0
36-37	37.37064657217529	38.0	38.0	38.0	37.0	38.0
38-39	37.37065580537358	38.0	38.0	38.0	37.0	38.0
40-41	37.37617344158254	38.0	38.0	38.0	37.0	38.0
42-43	37.34583582249428	38.0	38.0	38.0	37.0	38.0
44-45	37.315074715072754	38.0	38.0	38.0	37.0	38.0
46-47	37.29351516268031	38.0	38.0	38.0	37.0	38.0
48-49	37.24282770138424	38.0	38.0	38.0	37.0	38.0
50-51	37.20201999282252	38.0	38.0	38.0	37.0	38.0
52-53	37.19043134611982	38.0	38.0	38.0	37.0	38.0
54-55	37.17456025470449	38.0	38.0	38.0	37.0	38.0
56-57	37.1297522319053	38.0	38.0	38.0	36.0	38.0
58-59	37.112522925574645	38.0	38.0	38.0	36.0	38.0
60-61	37.13471995414683	38.0	38.0	38.0	36.0	38.0
62-63	37.14400106896494	38.0	38.0	38.0	36.0	38.0
64-65	37.1529168981409	38.0	38.0	38.0	36.0	38.0
66-67	37.154005798428244	38.0	38.0	38.0	36.0	38.0
68-69	37.144546605096295	38.0	38.0	38.0	36.0	38.0
70-71	37.14238131216574	38.0	38.0	38.0	36.0	38.0
72-73	37.14125646162546	38.0	38.0	38.0	36.0	38.0
74-75	37.11791894149045	38.0	38.0	38.0	36.0	38.0
76-77	37.09710570127278	38.0	38.0	38.0	36.0	38.0
78-79	37.09712965647981	38.0	38.0	38.0	36.0	38.0
80-81	37.08928523622936	38.0	38.0	38.0	36.0	38.0
82-83	37.0242769317593	38.0	38.0	38.0	36.0	38.0
84-85	37.03361729690103	38.0	38.0	38.0	36.0	38.0
86-87	37.03819573990447	38.0	38.0	38.0	36.0	38.0
88-89	37.02291044289579	38.0	38.0	38.0	36.0	38.0
90-91	37.010260392341976	38.0	38.0	38.0	36.0	38.0
92-93	37.000987864053954	38.0	38.0	38.0	36.0	38.0
94-95	36.949338317115966	38.0	38.0	38.0	35.5	38.0
96-97	36.868121747989946	38.0	38.0	38.0	35.0	38.0
98-99	36.86038280228031	38.0	38.0	38.0	35.0	38.0
100	35.54676391194075	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	11.0
21	70.0
22	308.0
23	881.0
24	2388.0
25	4711.0
26	8837.0
27	14421.0
28	23072.0
29	34436.0
30	46762.0
31	60731.0
32	78468.0
33	105126.0
34	158151.0
35	290599.0
36	890236.0
37	4541141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64847013345899	20.61827214133395	13.824370841885836	23.90888688332122
2	15.385648802283056	20.376164031031777	41.487228106552124	22.75095906013305
3	18.632477048714982	25.867976378039543	29.913482309487684	25.58606426375779
4	13.197792455693373	17.5526927408212	38.0647567628008	31.184758040684624
5	13.944594445004407	37.171214311283386	35.240762213340574	13.64342903037164
6	30.940056175970334	37.2203181686327	18.232974374505616	13.606651280891352
7	27.334318368781297	32.43801065435639	22.490899071138195	17.736771905724122
8	24.687629285902545	36.873018281725464	20.621770348303208	17.817582084068782
9	25.88847268922664	17.068135168161525	21.02157227631043	36.0218198663014
10-11	24.635835057145368	26.62283258923223	28.470876228964837	20.270456124657567
12-13	25.447946201090993	24.179630531839276	28.63585103069317	21.73657223637656
14-15	22.604343207648135	25.618495771003218	26.222104195452328	25.555056825896315
16-17	22.190644293050706	28.721852612074407	26.955218158729146	22.132284936145744
18-19	22.176978922903672	27.814107837421233	28.66870063175382	21.340212607921284
20-21	23.011319754101045	26.946509709206996	28.597294536439577	21.444876000252382
22-23	22.779476457922563	26.792358702887455	28.468429122972534	21.959735716217455
24-25	22.493284785482164	26.94924138124942	28.57102918909731	21.98644464417111
26-27	22.415360526610108	27.08381664509738	28.548962277716534	21.95186055057598
28-29	22.34229005805873	27.01519912980233	28.649720742851905	21.992790069287036
30-31	22.55127107007127	26.96841926236921	28.499861564130924	21.980448103428593
32-33	22.316721691352605	27.191688365836985	28.432492026933033	22.05909791587738
34-35	22.51944897806201	27.078453192959024	28.49549957355576	21.90659825542321
36-37	22.511371003343594	26.95176639172872	28.46711377208353	22.069748832844148
38-39	22.258570625474363	27.123109584427656	28.514840616780408	22.10347917331758
40-41	22.63277255410044	26.936501917464394	28.519183377110796	21.91154215132437
42-43	22.381857501672858	27.019899889922637	28.327054195308506	22.271188413096002
44-45	22.38136517731774	27.0990831304251	28.27556697612347	22.243984716133685
46-47	22.55246573017462	27.013095279621346	28.175826352555283	22.25861263764875
48-49	22.4480532529091	27.279273817826283	27.989608117623817	22.2830648116408
50-51	22.369265801490233	27.44537088249257	27.77543750392845	22.409925812088748
52-53	22.493233124311207	27.223624640735217	27.68445873789848	22.59868349705509
54-55	22.636080283530568	26.978770070968096	27.99030379593674	22.394845849564597
56-57	22.439971302868422	26.724353478380962	28.132923457407284	22.702751761343336
58-59	22.196834265420446	26.47594537227011	28.25004704239055	23.077173319918902
60-61	22.40110846695222	26.847351559932136	28.49188548484312	22.259654488272524
62-63	21.91597461591368	27.131044089344208	28.540291581577627	22.412689713164486
64-65	21.72919771333375	27.50949393154159	28.14297532397962	22.618333031145042
66-67	21.84307968444526	28.22870172027361	27.536069624419568	22.39214897086157
68-69	21.951248109579204	28.166990176211335	27.401862580806903	22.479899133402554
70-71	21.954956739977543	27.457941016544936	27.464771784495152	23.12233045898237
72-73	22.234766406280862	26.966098148719848	27.34949844397657	23.44963700102272
74-75	22.22445495119912	27.035329211424237	27.611487181267808	23.128728656108834
76-77	22.649469750986288	26.843883723136656	26.931175611931813	23.57547091394524
78-79	22.8510297793736	26.880785299229203	26.383458285786908	23.884726635610285
80-81	22.654598944210935	27.35394808307768	26.520124316583416	23.47132865612797
82-83	22.689047195099203	27.03470209452324	26.611815958827744	23.664434751549816
84-85	22.68532185874936	27.503558211245107	26.55310454357254	23.25801538643299
86-87	23.256810261427038	27.36143028530318	26.647498171890515	22.734261281379265
88-89	22.96715743592392	27.566503508103153	26.975960455864843	22.490378600108084
90-91	22.9427748977681	27.099470687080096	26.804013300363593	23.15374111478821
92-93	22.929276512091807	26.638368472871086	27.05012206065598	23.382232954381127
94-95	22.13845718815218	27.098857006948478	27.748877884945543	23.0138079199538
96-97	22.309327838101588	26.92708254214533	27.933133198781174	22.83045642097191
98-99	23.564042026638845	28.799496377067097	28.190403390951356	19.446058205342695
100	30.464682912864134	40.05943203492811	29.475885052207758	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1032.0
1	929.5
2	1072.5
3	1446.0
4	1883.0
5	2669.5
6	3330.5
7	4169.0
8	5258.5
9	6077.0
10	6388.0
11	6491.0
12	7357.5
13	9429.5
14	13280.0
15	17425.5
16	20282.5
17	21800.5
18	21645.5
19	20234.5
20	18299.5
21	16595.0
22	16245.0
23	18092.0
24	22306.0
25	28886.0
26	38407.5
27	49555.5
28	60725.5
29	73801.0
30	86759.0
31	99238.0
32	115192.5
33	132805.0
34	148630.5
35	164581.0
36	183386.5
37	198878.5
38	210208.0
39	219930.0
40	230740.5
41	239829.5
42	244790.5
43	252114.5
44	260450.5
45	267915.5
46	270751.0
47	270612.5
48	271269.0
49	270378.5
50	266515.0
51	260456.5
52	251450.0
53	239001.0
54	224243.5
55	207385.0
56	188155.0
57	168650.0
58	149257.5
59	127920.0
60	107129.5
61	86542.0
62	67992.0
63	52528.0
64	39094.5
65	28839.5
66	21222.5
67	16180.0
68	12491.0
69	9446.5
70	6965.0
71	4892.0
72	3497.5
73	2355.5
74	1482.0
75	999.0
76	672.0
77	440.0
78	293.0
79	179.5
80	100.5
81	63.5
82	43.5
83	31.5
84	26.0
85	21.0
86	18.5
87	19.0
88	15.5
89	7.5
90	3.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0050316675585230855
3	0.003881572116574952
4	0.0
5	4.792064341450558E-5
6	3.5141805170637425E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.11517247951195E-4
26-27	0.0021648702903186924
28-29	0.007767306209505825
30-31	0.002919827613377706
32-33	0.006117488818144457
34-35	0.0032975551282853504
36-37	0.0013896443059970907
38-39	4.8714703152896783E-5
40-41	8.978031491506497E-5
42-43	0.0025805136701478946
44-45	7.374409456876401E-4
46-47	4.8516600749614946E-4
48-49	0.0020685395815048923
50-51	4.4288043301441966E-4
52-53	0.0
54-55	0.005468513155704634
56-57	0.05039217233609954
58-59	0.07504025329592841
60-61	0.0352519575949619
62-63	0.014890628205440251
64-65	0.0016753845139034107
66-67	0.0
68-69	0.0023538804121349247
70-71	0.0037183327647269727
72-73	0.013879382497312354
74-75	0.016933448500480352
76-77	0.02274836853438949
78-79	0.006523997894596518
80-81	0.00845574139120683
82-83	0.03566642439325158
84-85	0.020283995411512896
86-87	0.01481951484096673
88-89	0.006915128390552123
90-91	6.039393757602087E-5
92-93	0.0
94-95	0.0
96-97	8.381129342511927E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	141.0
22-23	405.0
24-25	522.0
26-27	1448.0
28-29	4264.0
30-31	13379.0
32-33	17835.0
34-35	24889.0
36-37	38334.0
38-39	22677.0
40-41	41422.0
42-43	46910.0
44-45	56356.0
46-47	56179.0
48-49	52677.0
50-51	26615.0
52-53	3653.0
54-55	3668.0
56-57	3282.0
58-59	28256.0
60-61	52827.0
62-63	51073.0
64-65	54354.0
66-67	57779.0
68-69	59288.0
70-71	60045.0
72-73	53627.0
74-75	53646.0
76-77	56660.0
78-79	58576.0
80-81	57668.0
82-83	54007.0
84-85	53166.0
86-87	55358.0
88-89	57282.0
90-91	58036.0
92-93	56778.0
94-95	65906.0
96-97	214931.0
98-99	1533503.0
100-101	3052899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88523287897098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58846499829272	78.70799877525175
2	6.844642924969261	11.893967890187104
3	1.5303993841088852	3.9890732065840275
4	0.5035672111724464	1.7501021765172788
5	0.2167691111861036	0.9417017353186082
6	0.107251122192465	0.5591123236933977
7	0.06376472440607937	0.38781490506399274
8	0.04016837976078242	0.279203122390922
9	0.02357192010073759	0.18432465906213744
>10	0.07861491830351587	1.0457722357154773
>50	0.001966292006066737	0.11920199769345505
>100	7.9170896072247E-4	0.11899975089562807
>500	2.7304540256699615E-5	0.022727221626246075
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1947095609670386E-5	0.0	0.0	0.0
3	0.0	3.1947095609670386E-5	0.0	0.0	0.0
4	0.0	3.1947095609670386E-5	0.0	0.0	1.5973547804835193E-5
5	0.0	3.1947095609670386E-5	0.0	0.0	1.5973547804835193E-5
6	1.1181483463384635E-4	4.7920643414505575E-5	0.0	0.0	4.7920643414505575E-5
7	1.1181483463384635E-4	4.7920643414505575E-5	0.0	0.0	7.986773902417596E-5
8	1.1181483463384635E-4	6.389419121934077E-5	0.0	0.0	7.986773902417596E-5
9	1.2778838243868154E-4	1.5973547804835192E-4	0.0	0.0	7.986773902417596E-5
10-11	1.3577515634109914E-4	1.5973547804835192E-4	0.0	0.0	7.986773902417596E-5
12-13	1.5973547804835192E-4	1.7570902585318713E-4	0.0	0.0	7.986773902417596E-5
14-15	1.916825736580223E-4	2.3960321707252788E-4	0.0	7.986773902417596E-6	7.986773902417596E-5
16-17	2.635635387797807E-4	2.555767648773631E-4	0.0	1.5973547804835193E-5	7.986773902417596E-5
18-19	2.8752386048703344E-4	2.635635387797807E-4	0.0	4.7920643414505575E-5	8.785451292659356E-5
20-21	3.1148418219428624E-4	2.875238604870335E-4	0.0	6.389419121934077E-5	9.584128682901115E-5
22-23	3.1947095609670384E-4	3.2745772999912145E-4	0.0	1.1980160853626394E-4	1.1181483463384634E-4
24-25	3.3544450390153905E-4	3.3544450390153905E-4	0.0	1.5973547804835192E-4	1.3577515634109914E-4
26-27	3.5141805170637425E-4	3.3544450390153905E-4	0.0	2.3161644317011028E-4	1.4376193024351675E-4
28-29	3.5141805170637425E-4	3.3544450390153905E-4	0.0	6.309551382909901E-4	1.4376193024351675E-4
30-31	3.5141805170637425E-4	3.833651473160446E-4	0.0	0.0020126670234092343	1.4376193024351675E-4
32-33	3.5940482560879185E-4	3.833651473160446E-4	0.0	0.0050716014280351735	1.4376193024351675E-4
34-35	3.6739159951120945E-4	4.232990168281326E-4	0.0	0.009216737083389905	1.5973547804835192E-4
36-37	3.833651473160446E-4	4.632328863402206E-4	0.0	0.0194717547740941	1.7570902585318713E-4
38-39	4.073254690232974E-4	5.031667558523086E-4	0.0	0.193990751315821	1.916825736580223E-4
40-41	4.232990168281326E-4	5.590741731692318E-4	0.0	0.37253508190436635	1.996693475604399E-4
42-43	4.312857907305502E-4	6.069948165837373E-4	0.0	0.39431501433625915	2.076561214628575E-4
44-45	4.312857907305502E-4	6.788757817054957E-4	0.0	0.425104027730079	2.076561214628575E-4
46-47	4.632328863402206E-4	7.347831990224189E-4	0.0	0.45975863969266895	2.076561214628575E-4
48-49	4.7920643414505577E-4	7.427699729248365E-4	0.0	0.4927040820401415	2.076561214628575E-4
50-51	5.031667558523086E-4	9.344525465828587E-4	0.0	0.5256335508398093	2.236296692676927E-4
52-53	5.111535297547262E-4	9.584128682901115E-4	0.0	1.0714097454615157	2.236296692676927E-4
54-55	5.191403036571438E-4	9.823731899973643E-4	0.0	1.80713538380442	2.236296692676927E-4
56-57	5.271270775595614E-4	0.0010063335117046171	0.0	2.6152771011205447	2.236296692676927E-4
58-59	5.35113851461979E-4	0.0010223070595094523	0.0	3.4455901027897804	2.236296692676927E-4
60-61	5.590741731692318E-4	0.0010542541551191228	0.0	3.6620955697365165	2.236296692676927E-4
62-63	5.830344948764846E-4	0.0010862012507287932	0.0	3.6688443936840596	2.236296692676927E-4
64-65	6.069948165837373E-4	0.0011341218941432988	0.0	3.6747066857284336	2.236296692676927E-4
66-67	6.389419121934077E-4	0.0011580822158505516	0.0	3.6807446867986613	2.236296692676927E-4
68-69	6.549154599982429E-4	0.0011820425375578044	0.0	3.6866069788430362	2.236296692676927E-4
70-71	6.708890078030781E-4	0.0011980160853626394	0.0	3.691734487688388	2.236296692676927E-4
72-73	7.188096512175837E-4	0.0011980160853626394	0.0	3.695584112709353	2.475899909749455E-4
74-75	7.188096512175837E-4	0.001206002859265057	0.0	3.699018425487393	2.635635387797807E-4
76-77	7.267964251200013E-4	0.0012619102765819802	0.0	3.7025805266478713	2.715503126821983E-4
78-79	7.347831990224189E-4	0.0012938573721916506	0.0	3.7049206514012796	2.715503126821983E-4
80-81	7.347831990224189E-4	0.0012938573721916506	0.0	3.7058551039478624	2.9551063438945104E-4
82-83	7.347831990224189E-4	0.0012938573721916506	0.0	3.7064700855383483	3.1947095609670384E-4
84-85	7.347831990224189E-4	0.0012938573721916506	0.0	3.7067735829466404	3.3544450390153905E-4
86-87	7.507567468272541E-4	0.0012938573721916506	0.0	3.7068694242334694	3.3544450390153905E-4
88	7.507567468272541E-4	0.0013098309199964858	0.0	3.7068694242334694	3.3544450390153905E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	6975	0.0	59.163036	94
GGTATCA	8390	0.0	51.49564	1
TATCTCG	8525	0.0	44.15633	94
GTATCAA	14925	0.0	36.573425	1
TCAACGC	17515	0.0	30.453379	4
ATCAACG	17990	0.0	29.697046	3
CAACGCA	18075	0.0	29.50987	5
TATCAAC	18455	0.0	29.32182	2
AACGCAG	18705	0.0	28.607794	6
CCGTCTT	4340	0.0	28.195606	90-91
TATGCCG	4285	0.0	27.503984	86-87
GCCGTCT	4635	0.0	26.705223	90-91
TCTCGTA	4355	0.0	26.322475	82-83
ATGCCGT	4680	0.0	26.300238	88-89
CTATCTC	10645	0.0	26.277733	92-93
TGCCGTC	4605	0.0	26.186783	88-89
TGGTATC	3980	0.0	24.818708	2
CTCGTAT	4630	0.0	24.816895	82-83
ACGCAGA	21690	0.0	24.611359	7
TCGTATG	4870	0.0	24.539139	84-85
>>END_MODULE