FastQCFastQC Report
Fri 27 May 2016
SRR2139615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6641461
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80200.048.6084821
GTATCAA145500.034.7468871
TCAACGC182000.029.4022254
ATCAACG183450.029.124223
CAACGCA187950.028.4264835
TATCAAC193500.027.8300512
AACGCAG194100.027.569326
ACGCAGA222000.024.1045277
TGGTATC38950.023.2055072
CGCAGAG231700.023.1318598
GTGGTAT40850.022.6431471
GCAGAGT269400.020.0358729
TACATGG198800.019.5884172
ACATGGG197900.019.2505113
GAGTACT161350.019.1609512-13
GTACATG197600.019.0875111
AGAGTAC242950.018.27962710-11
CAGAGTA262450.018.00770810-11
CATGGGG138850.017.3686144
GTACTTT177800.017.25760314-15
Produced by FastQC (version 0.11.5)