##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6641461
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.493100238034973	18.0	18.0	25.0	18.0	33.0
2	29.353326323831457	30.0	27.0	32.0	27.0	33.0
3	30.453951321855236	31.0	29.0	33.0	27.0	33.0
4	32.01339810622994	33.0	33.0	33.0	29.0	33.0
5	32.40725286198323	33.0	33.0	33.0	32.0	34.0
6	36.00657355362021	38.0	36.0	38.0	33.0	38.0
7	36.156455334150124	38.0	36.0	38.0	33.0	38.0
8	35.70756434465248	38.0	36.0	38.0	31.0	38.0
9	36.73957853550597	38.0	37.0	38.0	34.0	38.0
10-11	36.91859863364401	38.0	38.0	38.0	35.0	38.0
12-13	37.06776174700115	38.0	38.0	38.0	36.0	38.0
14-15	36.94397460137159	38.0	38.0	38.0	35.5	38.0
16-17	37.049019033613234	38.0	38.0	38.0	35.5	38.0
18-19	37.21044307871416	38.0	38.0	38.0	36.0	38.0
20-21	37.28467367274948	38.0	38.0	38.0	36.5	38.0
22-23	37.34860269119224	38.0	38.0	38.0	37.0	38.0
24-25	37.37752125465475	38.0	38.0	38.0	37.0	38.0
26-27	37.361979387146405	38.0	38.0	38.0	37.0	38.0
28-29	37.33674323136738	38.0	38.0	38.0	37.0	38.0
30-31	37.34857874678538	38.0	38.0	38.0	37.0	38.0
32-33	37.33789208564893	38.0	38.0	38.0	37.0	38.0
34-35	37.313402726519726	38.0	38.0	38.0	37.0	38.0
36-37	37.29423366629608	38.0	38.0	38.0	37.0	38.0
38-39	37.27961073275647	38.0	38.0	38.0	37.0	38.0
40-41	37.27008223372661	38.0	38.0	38.0	37.0	38.0
42-43	37.24113963461221	38.0	38.0	38.0	37.0	38.0
44-45	37.204471116682264	38.0	38.0	38.0	36.5	38.0
46-47	37.16918935526731	38.0	38.0	38.0	36.0	38.0
48-49	37.113341102481066	38.0	38.0	38.0	36.0	38.0
50-51	37.0530017360734	38.0	38.0	38.0	36.0	38.0
52-53	37.020330779301005	38.0	38.0	38.0	36.0	38.0
54-55	36.983886797129436	38.0	38.0	38.0	36.0	38.0
56-57	36.915189918844035	38.0	38.0	38.0	36.0	38.0
58-59	36.880277041940296	38.0	38.0	38.0	35.5	38.0
60-61	36.89726507768253	38.0	38.0	38.0	35.5	38.0
62-63	36.89821503948273	38.0	38.0	38.0	35.0	38.0
64-65	36.898461013750115	38.0	38.0	38.0	35.0	38.0
66-67	36.88771823771826	38.0	38.0	38.0	35.0	38.0
68-69	36.869338425245104	38.0	38.0	38.0	35.0	38.0
70-71	36.854703580815226	38.0	38.0	38.0	35.0	38.0
72-73	36.84297742313857	38.0	38.0	38.0	35.0	38.0
74-75	36.80973533655418	38.0	38.0	38.0	35.0	38.0
76-77	36.777747639164424	38.0	38.0	38.0	35.0	38.0
78-79	36.774279985920074	38.0	38.0	38.0	35.0	38.0
80-81	36.76779932630441	38.0	38.0	38.0	35.0	38.0
82-83	36.70062794456794	38.0	38.0	38.0	34.0	38.0
84-85	36.67119345428017	38.0	38.0	38.0	34.0	38.0
86-87	36.66466913556809	38.0	38.0	38.0	34.0	38.0
88-89	36.642272394386744	38.0	38.0	38.0	34.0	38.0
90-91	36.60643691814438	38.0	38.0	38.0	34.0	38.0
92-93	36.58529173611991	38.0	38.0	38.0	34.0	38.0
94-95	36.55782040761482	38.0	38.0	38.0	34.0	38.0
96-97	36.51143313214321	38.0	38.0	38.0	34.0	38.0
98-99	36.49064786165994	38.0	37.5	38.0	34.0	38.0
100	34.92753501969044	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	34.0
21	130.0
22	444.0
23	1284.0
24	3101.0
25	6319.0
26	11068.0
27	18875.0
28	30157.0
29	44477.0
30	61377.0
31	81465.0
32	108971.0
33	155683.0
34	248209.0
35	481232.0
36	1535112.0
37	3853512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.55822371011439	16.799074781889107	19.955488709487266	23.687212798509243
2	14.862732414411598	20.941290977528066	41.032367041083454	23.163609566976877
3	18.53903332402789	24.942894992737838	31.068737715082978	25.449333968151294
4	12.883400203660008	17.16144986773242	38.127137989668235	31.828011938939337
5	13.691871018100088	36.8482286798729	35.47901146419785	13.98088883782916
6	30.992569393731788	37.028540454268274	18.24577464258558	13.73311550941436
7	26.99738807470224	32.24849170988131	22.841736178229457	17.912384037187
8	24.553302353202106	36.685949070543366	20.877063043809187	17.883685532445345
9	25.659293941498717	16.531362602294884	21.224441429378267	36.58490202682813
10-11	24.56138039506669	26.36757936243245	28.682815422690883	20.388224819809977
12-13	25.19392946822996	24.033785638431063	28.915482602397276	21.856802290941708
14-15	22.29474809834764	25.37596019911884	26.629291356224183	25.70000034630934
16-17	21.92324098568071	28.448130614634344	27.33511496943218	22.29351343025277
18-19	21.943560309998055	27.61394669034419	29.103001583537115	21.33949141612064
20-21	22.70140559133257	26.744894519856864	29.06473266767233	21.488967221138232
22-23	22.49345322786622	26.600734912910728	28.940896323914604	21.96491553530845
24-25	22.23271581039138	26.68922800672172	29.054976212107313	22.023079970779584
26-27	22.10430116215245	26.85657518996889	29.01023998730064	22.028883660578018
28-29	22.04809636540599	26.85153257130743	29.09875837355954	22.00161268972704
30-31	22.316545535168057	26.765504257333646	28.957381106804924	21.960569100693377
32-33	22.06440463644668	26.965364259511208	28.908745001632415	22.061486102409695
34-35	22.24430709097224	26.908403822242267	28.924697980254688	21.92259110653081
36-37	22.22084539895385	26.812556079510475	28.912473211007413	22.054125310528264
38-39	22.064012705866105	26.99134911592185	29.014029393824302	21.930608784387744
40-41	22.2740263390357	26.825704867379425	28.89061577864632	22.00965301493855
42-43	22.192718492699655	26.92643769060366	28.70605121371428	22.174792602982407
44-45	22.22487303197002	27.01292626944086	28.59865479124834	22.163545907340776
46-47	22.262002718797863	27.02378365428028	28.383174657512257	22.3310389694096
48-49	22.260535469184116	27.07294771246382	28.365573534418242	22.30094328393382
50-51	22.22262608991775	27.283750657900473	28.233065908300066	22.26055734388171
52-53	22.414674781322827	27.15098374191615	28.125784770525364	22.308556706235656
54-55	22.391098290123658	27.085096677430816	28.152612983515418	22.371192048930116
56-57	22.29226204867652	27.175991049877616	28.17848990456577	22.3532569968801
58-59	22.290699000443777	27.128057501988977	28.226878762047164	22.35436473552008
60-61	22.268990042014273	27.114276205985927	28.285631253793454	22.33110249820635
62-63	22.26123500478085	27.175015819155668	28.32380779736502	22.239941378698457
64-65	22.171767503350154	27.07095801325635	28.372153334675826	22.38512114871767
66-67	22.234025541650958	27.16530305085263	28.204612787336636	22.396058620159774
68-69	22.213201687655765	27.117820924670994	28.332167312906293	22.33681007476695
70-71	22.144034090808468	26.84211076254134	28.335018133144995	22.6788370135052
72-73	22.3377892132041	26.916076058308747	28.30792271067318	22.438212017813978
74-75	22.201488441328735	27.184802586327855	28.20560449938505	22.408104472958364
76-77	22.393988179330453	27.094050259342197	28.002402873443767	22.50955868788359
78-79	22.331619855310606	27.133519082358852	27.938695994605585	22.596165067724957
80-81	22.352216559193934	27.183922742407923	27.894764388283328	22.569096310114816
82-83	22.447002543968463	27.188337446227013	27.927205367052643	22.437454642751877
84-85	22.471517677542305	27.219060750149993	27.852288280243286	22.457133292064412
86-87	22.425103040576015	27.25192763375527	27.918298114356976	22.40467121131174
88-89	22.493718919683406	27.259956756634608	27.83674275652201	22.40958156715998
90-91	22.52644811993796	27.284419217557847	27.846278538178264	22.34285412432592
92-93	22.51859281569094	27.32600915973776	27.824902336485042	22.33049568808626
94-95	22.41664663924365	27.37059500573772	27.8038030537034	22.408955301315228
96-97	22.590564531632705	27.4402122029419	27.855680157622864	22.11354310780253
98-99	23.580911498720294	29.38062703552365	28.080949805956333	18.95751165979972
100	30.052151176865493	40.400165515203945	29.547683307930555	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1767.0
1	1636.0
2	1917.0
3	2455.0
4	2892.5
5	3678.5
6	4672.5
7	6983.0
8	9056.0
9	9471.5
10	9222.5
11	8823.0
12	9405.0
13	11389.0
14	15822.0
15	21053.0
16	24289.5
17	25386.5
18	24931.0
19	23381.0
20	21557.0
21	20497.0
22	21280.5
23	24802.0
24	30942.0
25	39785.0
26	51231.0
27	63989.5
28	76306.0
29	90410.5
30	104038.0
31	116527.0
32	132180.0
33	149446.5
34	165372.0
35	180300.0
36	197196.0
37	211631.5
38	223629.5
39	234588.5
40	244448.5
41	254360.5
42	260865.0
43	266786.0
44	273907.5
45	279457.0
46	283045.0
47	285505.5
48	286348.0
49	284746.0
50	281153.5
51	275120.0
52	264533.5
53	250967.0
54	235462.0
55	218545.0
56	201171.0
57	182842.5
58	163393.0
59	141695.0
60	119308.5
61	97500.5
62	77811.5
63	60798.0
64	45910.5
65	34461.5
66	25794.0
67	19689.0
68	15080.0
69	11271.0
70	8451.0
71	6109.5
72	4312.0
73	2897.0
74	1951.0
75	1316.5
76	860.5
77	550.0
78	371.5
79	241.5
80	153.0
81	103.0
82	66.5
83	42.0
84	30.0
85	22.0
86	15.0
87	10.0
88	7.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0029963286692491304
3	0.002815645533415012
4	0.0
5	3.011385597235307E-5
6	1.656262078479419E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6564833100271152E-4
26-27	0.001506068212389655
28-29	0.0058459033438642465
30-31	0.0021043167909395206
32-33	0.004914388331034408
34-35	0.0023285278182467993
36-37	0.0010668996369035707
38-39	2.3017868080454816E-5
40-41	7.727722656361388E-5
42-43	0.0020003740777360932
44-45	4.474693371636708E-4
46-47	3.32971161129898E-4
48-49	0.0019371041493651381
50-51	6.219523828409315E-4
52-53	0.0
54-55	0.004645941880580414
56-57	0.04593217519896008
58-59	0.06949488630258965
60-61	0.031758959783593804
62-63	0.01388743873507898
64-65	0.0015886414710991768
66-67	0.0
68-69	0.002251675658474948
70-71	0.0035772675626128078
72-73	0.012675374359623001
74-75	0.015745115220278046
76-77	0.021300379984542164
78-79	0.0064021839011643026
80-81	0.008293117775705587
82-83	0.033397062209172754
84-85	0.019356548674953226
86-87	0.013734930104038725
88-89	0.006817797099631195
90-91	2.9565828761224296E-5
92-93	0.0
94-95	1.0093618309823614E-5
96-97	3.0792121158374965E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	232.0
22-23	485.0
24-25	628.0
26-27	1887.0
28-29	5320.0
30-31	15278.0
32-33	19519.0
34-35	27102.0
36-37	42604.0
38-39	46752.0
40-41	45612.0
42-43	51789.0
44-45	61613.0
46-47	61482.0
48-49	57601.0
50-51	51339.0
52-53	48021.0
54-55	52363.0
56-57	53051.0
58-59	55812.0
60-61	54084.0
62-63	52769.0
64-65	55762.0
66-67	58371.0
68-69	60130.0
70-71	58559.0
72-73	54568.0
74-75	55522.0
76-77	58012.0
78-79	60197.0
80-81	59549.0
82-83	55453.0
84-85	54829.0
86-87	56744.0
88-89	59673.0
90-91	60146.0
92-93	59214.0
94-95	68167.0
96-97	205283.0
98-99	1527592.0
100-101	3168289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.59987680022093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.23573199548972	79.92238882582873
2	6.362734194930936	11.147494631770055
3	1.3753922964098477	3.614525871524268
4	0.5023221969782395	1.7601345027724036
5	0.2062406380581801	0.9033327242547767
6	0.10665721056362955	0.5605895103137502
7	0.06594534457083064	0.40437628419670163
8	0.04118682068280575	0.2886368334085259
9	0.022288920535514196	0.1757256023628862
>10	0.07974298735418854	1.0537475949745227
>50	0.0014981117032447124	0.08910814886634469
>100	1.8376170712073724E-4	0.03186588057848548
>500	7.552101573840137E-5	0.048073589148551955
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.011385597235307E-5	0.0	3.011385597235307E-5	0.0
3	0.0	3.011385597235307E-5	0.0	3.011385597235307E-5	0.0
4	0.0	7.528463993088268E-5	0.0	3.011385597235307E-5	0.0
5	0.0	9.034156791705921E-5	0.0	4.5170783958529607E-5	0.0
6	0.0	9.034156791705921E-5	0.0	4.5170783958529607E-5	3.011385597235307E-5
7	0.0	9.034156791705921E-5	0.0	4.5170783958529607E-5	6.022771194470614E-5
8	0.0	1.2045542388941228E-4	0.0	4.5170783958529607E-5	6.022771194470614E-5
9	0.0	2.2585391979264803E-4	0.0	4.5170783958529607E-5	1.0539849590323575E-4
10-11	1.5056927986176536E-5	2.2585391979264803E-4	0.0	4.5170783958529607E-5	1.0539849590323575E-4
12-13	1.5056927986176536E-5	2.2585391979264803E-4	0.0	6.775617593779441E-5	1.0539849590323575E-4
14-15	1.5056927986176536E-5	3.8395166364750164E-4	0.0	7.528463993088268E-5	1.2798388788250057E-4
16-17	1.5056927986176536E-5	3.914801276405899E-4	0.0	7.528463993088268E-5	1.5056927986176536E-4
18-19	1.5056927986176536E-5	4.0653705562676643E-4	0.0	7.528463993088268E-5	1.5056927986176536E-4
20-21	1.5056927986176536E-5	4.291224476060313E-4	0.0	9.034156791705921E-5	1.5056927986176536E-4
22-23	1.5056927986176536E-5	4.5170783958529607E-4	0.0	1.0539849590323575E-4	1.5056927986176536E-4
24-25	1.5056927986176536E-5	5.119355515300023E-4	0.0	1.3551235187558882E-4	1.5056927986176536E-4
26-27	1.5056927986176536E-5	5.571063354885318E-4	0.0	1.9574006382029496E-4	1.5056927986176536E-4
28-29	1.5056927986176536E-5	5.571063354885318E-4	0.0	6.926186873641206E-4	1.656262078479419E-4
30-31	1.5056927986176536E-5	5.872201914608849E-4	0.0	0.0022284253419541274	1.7315467184103014E-4
32-33	1.5056927986176536E-5	5.872201914608849E-4	0.0	0.005864673450615761	1.9574006382029496E-4
34-35	2.2585391979264803E-5	7.001471513572089E-4	0.0	0.01081840275806784	2.2585391979264803E-4
36-37	3.011385597235307E-5	8.13074111253533E-4	0.0	0.019031956974527142	2.2585391979264803E-4
38-39	3.011385597235307E-5	8.13074111253533E-4	0.0	0.03775524692533767	2.2585391979264803E-4
40-41	3.011385597235307E-5	8.733018231982391E-4	0.0	0.0580745712426829	2.2585391979264803E-4
42-43	3.011385597235307E-5	9.109441431636805E-4	0.0	0.0710310577747878	2.333823837857363E-4
44-45	3.764231996544134E-5	0.0010765703510116223	0.0	0.08541795246557948	2.4091084777882457E-4
46-47	4.5170783958529607E-5	0.0012196111668802993	0.0	0.10075343361950029	2.4091084777882457E-4
48-49	4.5170783958529607E-5	0.0012271396308733877	0.0	0.11545652379800167	2.5596777576500113E-4
50-51	4.5170783958529607E-5	0.0015282781905969183	0.0	0.1308597611278603	2.5596777576500113E-4
52-53	4.5170783958529607E-5	0.0015358066545900066	0.0	0.17097894574702766	2.6349623975808936E-4
54-55	4.5170783958529607E-5	0.001550863582576183	0.0	0.2218337200203389	2.7102470375117764E-4
56-57	4.5170783958529607E-5	0.0015659205105623597	0.0	0.293060517858947	2.7102470375117764E-4
58-59	4.5170783958529607E-5	0.0015885059025416244	0.0	0.33921753060057114	2.7102470375117764E-4
60-61	4.5170783958529607E-5	0.001603562830527801	0.0	0.35751922656776874	2.7102470375117764E-4
62-63	4.5170783958529607E-5	0.0016110912945208893	0.0	0.37161251116283	2.860816317373542E-4
64-65	4.5170783958529607E-5	0.0017466036463964782	0.0	0.3784558849325472	2.860816317373542E-4
66-67	4.5170783958529607E-5	0.0017616605743826547	0.0	0.3851938602063612	2.860816317373542E-4
68-69	4.5170783958529607E-5	0.0017917744303550076	0.0	0.39159305460048627	2.860816317373542E-4
70-71	4.5170783958529607E-5	0.0018143598223342725	0.0	0.396855450931655	2.860816317373542E-4
72-73	4.5170783958529607E-5	0.0018218882863273607	0.0	0.40156826939132817	3.764231996544134E-4
74-75	4.5170783958529607E-5	0.0018444736783066256	0.0	0.4054981275957203	3.764231996544134E-4
76-77	4.5170783958529607E-5	0.0018745875342789787	0.0	0.40907414799243724	3.764231996544134E-4
78-79	4.5170783958529607E-5	0.001882115998272067	0.0	0.41137032951032915	3.764231996544134E-4
80-81	4.5170783958529607E-5	0.0018971729262582436	0.0	0.4121532897656103	3.8395166364750164E-4
82-83	4.5170783958529607E-5	0.00191222985424442	0.0	0.41271792456509193	3.914801276405899E-4
84-85	4.5170783958529607E-5	0.0019197583182375083	0.0	0.4129061361649191	3.914801276405899E-4
86-87	4.5170783958529607E-5	0.001979986030182215	0.0	0.4130567054447809	3.914801276405899E-4
88	4.5170783958529607E-5	0.0021531407020232447	0.0	0.4130717623727671	4.065370556267665E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8020	0.0	48.608482	1
GTATCAA	14550	0.0	34.746887	1
TCAACGC	18200	0.0	29.402225	4
ATCAACG	18345	0.0	29.12422	3
CAACGCA	18795	0.0	28.426483	5
TATCAAC	19350	0.0	27.830051	2
AACGCAG	19410	0.0	27.56932	6
ACGCAGA	22200	0.0	24.104527	7
TGGTATC	3895	0.0	23.205507	2
CGCAGAG	23170	0.0	23.131859	8
GTGGTAT	4085	0.0	22.643147	1
GCAGAGT	26940	0.0	20.035872	9
TACATGG	19880	0.0	19.588417	2
ACATGGG	19790	0.0	19.250511	3
GAGTACT	16135	0.0	19.16095	12-13
GTACATG	19760	0.0	19.087511	1
AGAGTAC	24295	0.0	18.279627	10-11
CAGAGTA	26245	0.0	18.007708	10-11
CATGGGG	13885	0.0	17.368614	4
GTACTTT	17780	0.0	17.257603	14-15
>>END_MODULE