FastQCFastQC Report
Fri 27 May 2016
SRR2139614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5801198
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75450.049.5349351
GTATCAA134800.034.7910581
ATCAACG168950.028.991873
TCAACGC170100.028.795624
CAACGCA173900.028.1419375
TATCAAC176900.027.8812452
AACGCAG180100.027.1731416
ACGCAGA204500.023.9309677
TGGTATC35700.023.5819112
CGCAGAG212650.023.0337898
GTGGTAT38800.022.9032211
GCAGAGT245850.019.8886789
GAGTACT146100.019.55677812-13
TACATGG170150.018.8417572
CAGAGTA238600.018.43479510-11
GTACATG164350.018.2907581
AGAGTAC223100.017.95269210-11
ACATGGG171800.017.8193283
GTACTTT161000.017.57528114-15
AGTACTT160200.016.4022812-13
Produced by FastQC (version 0.11.5)