##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139614_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5801198
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.464952928688177	18.0	18.0	25.0	18.0	33.0
2	29.332872451517773	30.0	27.0	32.0	27.0	33.0
3	30.486882881777177	31.0	29.0	33.0	27.0	33.0
4	32.03328502147315	33.0	33.0	33.0	30.0	33.0
5	32.430446090617835	33.0	33.0	33.0	32.0	34.0
6	36.02081656237212	38.0	36.0	38.0	33.0	38.0
7	36.157985126520416	38.0	36.0	38.0	33.0	38.0
8	35.72182762939655	38.0	36.0	38.0	31.0	38.0
9	36.738932199866305	38.0	37.0	38.0	34.0	38.0
10-11	36.91610706960873	38.0	38.0	38.0	35.0	38.0
12-13	37.06787442869559	38.0	38.0	38.0	36.0	38.0
14-15	36.9470713635356	38.0	38.0	38.0	35.5	38.0
16-17	37.053624785777004	38.0	38.0	38.0	35.5	38.0
18-19	37.21219298151864	38.0	38.0	38.0	36.0	38.0
20-21	37.2865515810105	38.0	38.0	38.0	36.5	38.0
22-23	37.34795692064175	38.0	38.0	38.0	37.0	38.0
24-25	37.375600975336965	38.0	38.0	38.0	37.0	38.0
26-27	37.36084264696797	38.0	38.0	38.0	37.0	38.0
28-29	37.3370814073111	38.0	38.0	38.0	37.0	38.0
30-31	37.34733974225839	38.0	38.0	38.0	37.0	38.0
32-33	37.337802100404176	38.0	38.0	38.0	37.0	38.0
34-35	37.31433055791303	38.0	38.0	38.0	37.0	38.0
36-37	37.29247905215786	38.0	38.0	38.0	37.0	38.0
38-39	37.273675849736485	38.0	38.0	38.0	37.0	38.0
40-41	37.26545096309495	38.0	38.0	38.0	37.0	38.0
42-43	37.2373200953326	38.0	38.0	38.0	37.0	38.0
44-45	37.19984918585855	38.0	38.0	38.0	36.0	38.0
46-47	37.16483377237982	38.0	38.0	38.0	36.0	38.0
48-49	37.11012861207737	38.0	38.0	38.0	36.0	38.0
50-51	37.04539425684284	38.0	38.0	38.0	36.0	38.0
52-53	37.01694740925923	38.0	38.0	38.0	36.0	38.0
54-55	36.98092856628667	38.0	38.0	38.0	36.0	38.0
56-57	36.91641396736941	38.0	38.0	38.0	36.0	38.0
58-59	36.87836604103642	38.0	38.0	38.0	35.5	38.0
60-61	36.89682404683491	38.0	38.0	38.0	35.5	38.0
62-63	36.89879859827276	38.0	38.0	38.0	35.0	38.0
64-65	36.89880390384116	38.0	38.0	38.0	35.0	38.0
66-67	36.89092947105599	38.0	38.0	38.0	35.0	38.0
68-69	36.87236956536484	38.0	38.0	38.0	35.0	38.0
70-71	36.85777936663112	38.0	38.0	38.0	35.0	38.0
72-73	36.84765454700185	38.0	38.0	38.0	35.0	38.0
74-75	36.817827656167594	38.0	38.0	38.0	35.0	38.0
76-77	36.788593851504565	38.0	38.0	38.0	35.0	38.0
78-79	36.78675726104479	38.0	38.0	38.0	35.0	38.0
80-81	36.78079212784742	38.0	38.0	38.0	35.0	38.0
82-83	36.7161481481193	38.0	38.0	38.0	34.5	38.0
84-85	36.68923571938308	38.0	38.0	38.0	34.0	38.0
86-87	36.68390129767107	38.0	38.0	38.0	34.0	38.0
88-89	36.65949771674035	38.0	38.0	38.0	34.0	38.0
90-91	36.6241205512626	38.0	38.0	38.0	34.0	38.0
92-93	36.60404943890863	38.0	38.0	38.0	34.0	38.0
94-95	36.58096906587268	38.0	38.0	38.0	34.0	38.0
96-97	36.53435526481712	38.0	38.0	38.0	34.0	38.0
98-99	36.50833371014065	38.0	37.5	38.0	34.0	38.0
100	34.95595464984892	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	8.0
20	19.0
21	106.0
22	411.0
23	1132.0
24	2717.0
25	5318.0
26	9936.0
27	16788.0
28	26737.0
29	39374.0
30	53534.0
31	71160.0
32	95080.0
33	134655.0
34	212423.0
35	412808.0
36	1324895.0
37	3394093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.810207822591124	16.91012787358749	20.92864267001402	23.351021633807363
2	14.703716913378898	20.902140881013572	41.632225902224484	22.761916303383043
3	18.5739606271007	25.08356251613933	31.14936513173659	25.193111725023382
4	12.84336442231415	17.542238689319	38.427614434122056	31.186782454244792
5	13.687175250211292	36.772031703112305	35.61078860104217	13.930004445634236
6	30.535753594131414	37.27805642735899	18.53199850444555	13.654191474064042
7	26.818270984717294	32.28702071537637	22.896822346005084	17.997885953901246
8	24.274072355399696	36.87910669485854	21.00360994401501	17.843211005726747
9	25.33805948357563	17.07955839466262	21.47864975475755	36.10373236700419
10-11	24.322174488786626	26.55845396071639	28.86784764112516	20.251523909371823
12-13	25.086145999498726	24.144745619784054	29.096016029792466	21.673092350924758
14-15	22.221323940330944	25.621518176073288	26.7719357277583	25.385222155837468
16-17	21.749119750782512	28.763619169695637	27.52677119450155	21.9604898850203
18-19	21.732450779994064	27.77293586600561	29.3760443963471	21.11856895765323
20-21	22.435130397698305	26.869921722853963	29.42945399236371	21.265493887084016
22-23	22.24261871287657	26.753848950526	29.29907757237342	21.70445476422401
24-25	22.01923222802415	26.88791469782607	29.390057665306752	21.702795408843027
26-27	21.89149050926038	27.066514493888143	29.35375308558529	21.688241911266186
28-29	21.86579182708176	27.010427818532655	29.41241307743505	21.711367276950533
30-31	22.106970736522342	26.907183173806814	29.341242409346822	21.644603680324014
32-33	21.895026484834247	27.08481341229241	29.28244733917994	21.737712763693402
34-35	22.065861704734992	27.003854187226306	29.308844676471672	21.62143943156703
36-37	22.005449200045167	26.976493790365414	29.257518613569612	21.76053839601981
38-39	21.839877434304054	27.05511687061265	29.38296573149957	21.722039963583725
40-41	22.12079464515091	26.90619874315752	29.281049119052792	21.691957492638778
42-43	21.97557128233673	27.0094220080824	29.12884322314571	21.886163486435155
44-45	21.989378129910694	27.139465258103172	29.00122842337535	21.869928188610785
46-47	22.142728863003256	27.054185048928826	28.73967392345198	22.063412164615936
48-49	22.05805136103685	27.198156915595735	28.691847999684956	22.051943723682463
50-51	22.050828659799198	27.357660226131514	28.594076762039737	21.997434352029554
52-53	22.18829155499014	27.30976724387597	28.51423904072739	21.9877021604065
54-55	22.134622519601688	27.23125736719637	28.535862336167362	22.098257777034576
56-57	22.17055683069694	27.30025113050129	28.49142723576532	22.03776480303645
58-59	22.087294661700177	27.284045528417007	28.586909132537073	22.04175067734574
60-61	22.104924987323262	27.32549062103418	28.60670968604102	21.962874705601532
62-63	22.08147337697027	27.402420708952523	28.645538604942466	21.87056730913474
64-65	22.002387404304944	27.29628458622723	28.693215041367687	22.00811296810014
66-67	22.021799978156963	27.357929586980845	28.622564641670518	21.997705793191678
68-69	22.06515862175797	27.260947773506288	28.672434911135376	22.001458693600366
70-71	22.046700884899636	27.03373510560744	28.641048274677473	22.27851573481545
72-73	22.169027288980477	27.056030464456445	28.636074072001517	22.138868174561562
74-75	22.054443090899063	27.28905100957963	28.541936191689594	22.11456970783172
76-77	22.18713434940252	27.20400653575654	28.324023287534324	22.28483582730662
78-79	22.144978841485724	27.246870494522845	28.248130566326473	22.36002009766496
80-81	22.15653782435629	27.34141555365172	28.17735082919165	22.324695792800338
82-83	22.246659594655352	27.345974420225737	28.197645116232184	22.209720868886723
84-85	22.282921330594547	27.35217303813401	28.147610004925163	22.217295626346285
86-87	22.203521590071453	27.399604736803507	28.228684634507772	22.16818903861727
88-89	22.292353926143036	27.43115124153499	28.12121978647413	22.155275045847848
90-91	22.287606465499458	27.48108642438857	28.070501094781363	22.160806015330607
92-93	22.358253677523074	27.452236041340296	28.068061496831447	22.121448784305187
94-95	22.198677848094782	27.50680251593513	28.106482277041923	22.188037358928167
96-97	22.308187572036225	27.588376389654268	28.12488577154123	21.978550266768277
98-99	23.377536969219346	29.523742050173666	28.318774376452854	18.779946604154134
100	29.720858973886987	40.43491712425556	29.844223901857447	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1653.0
1	1501.5
2	1601.0
3	1918.5
4	2247.0
5	2896.5
6	3675.5
7	5708.0
8	7668.5
9	8102.5
10	8000.0
11	7851.5
12	8517.0
13	10580.0
14	14735.0
15	19534.0
16	22656.0
17	23602.5
18	22976.5
19	21298.0
20	19515.0
21	18436.0
22	18765.0
23	21674.5
24	26898.5
25	34464.5
26	44663.5
27	55974.5
28	68109.5
29	82141.0
30	95685.5
31	108334.5
32	123274.0
33	138994.0
34	152616.0
35	166098.0
36	181244.5
37	194290.0
38	204629.0
39	213007.5
40	221220.5
41	228268.5
42	232605.0
43	238702.5
44	244365.5
45	247655.5
46	247863.0
47	245677.0
48	243763.5
49	241143.5
50	236729.0
51	230066.0
52	220545.5
53	208457.5
54	194456.5
55	179608.5
56	163616.5
57	147150.5
58	130393.5
59	111534.5
60	92869.0
61	75551.0
62	59915.0
63	46296.5
64	34519.0
65	25658.5
66	19199.5
67	14574.5
68	11069.5
69	8124.5
70	5918.5
71	4237.5
72	2952.0
73	2027.0
74	1388.5
75	919.0
76	603.5
77	374.0
78	230.0
79	150.0
80	93.5
81	57.5
82	38.5
83	33.5
84	27.5
85	18.0
86	10.5
87	5.5
88	3.0
89	2.0
90	2.0
91	2.5
92	2.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0030166182916011486
3	0.002620148458990712
4	0.0
5	1.723781880914942E-5
6	8.61890940457471E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.5516232824176773E-4
26-27	0.0016552740133704757
28-29	0.005709199426216833
30-31	0.0022013294475983886
32-33	0.0044292266674048715
34-35	0.002238284856103009
36-37	0.0010890614407516163
38-39	6.135994859088193E-5
40-41	9.704384167279586E-5
42-43	0.001900367969848442
44-45	5.10199469195633E-4
46-47	2.8004678407372136E-4
48-49	0.0015770217897921111
50-51	4.412733235934505E-4
52-53	0.0
54-55	0.004273631080383268
56-57	0.04242989449902202
58-59	0.06424046042917599
60-61	0.03000161922614771
62-63	0.012441600968610972
64-65	0.001343736711734103
66-67	0.0
68-69	0.0021845172341032927
70-71	0.003732175101616493
72-73	0.011758137543449359
74-75	0.014684089466729128
76-77	0.018724418615585516
78-79	0.005596293416169613
80-81	0.007175801004063403
82-83	0.030955797296790065
84-85	0.017685218194879903
86-87	0.013175879936072502
88-89	0.006257461665624123
90-91	6.673118088943545E-5
92-93	0.0
94-95	0.0
96-97	2.3134872373006325E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	220.0
22-23	461.0
24-25	592.0
26-27	1477.0
28-29	3714.0
30-31	11493.0
32-33	15167.0
34-35	21385.0
36-37	33493.0
38-39	36679.0
40-41	36198.0
42-43	41728.0
44-45	50934.0
46-47	50511.0
48-49	47401.0
50-51	41513.0
52-53	39304.0
54-55	42722.0
56-57	43824.0
58-59	46105.0
60-61	45119.0
62-63	44517.0
64-65	47747.0
66-67	50521.0
68-69	51122.0
70-71	52578.0
72-73	47009.0
74-75	46875.0
76-77	49183.0
78-79	50727.0
80-81	49956.0
82-83	47068.0
84-85	46338.0
86-87	48448.0
88-89	50420.0
90-91	50992.0
92-93	50511.0
94-95	58543.0
96-97	178711.0
98-99	1344893.0
100-101	2824952.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.0391876883117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.06219394909307	81.97142966031052
2	5.88232708649835	10.475152509975326
3	1.224275420336612	3.2702546680061486
4	0.3955828322747866	1.408894961967546
5	0.18764593973016808	0.8353921023292031
6	0.08316377083386996	0.44429007600868137
7	0.052595581079696974	0.3278147470731668
8	0.030789277826008016	0.2193161829710001
9	0.018686801948629737	0.14974718993985167
>10	0.061809856464414066	0.8130342801452026
>50	7.105659150334414E-4	0.041631782919601956
>100	2.1891799936068842E-4	0.04304183835375926
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.723781880914942E-5	0.0	0.0	0.0
3	0.0	1.723781880914942E-5	0.0	0.0	0.0
4	0.0	1.723781880914942E-5	0.0	1.723781880914942E-5	0.0
5	0.0	3.447563761829884E-5	0.0	3.447563761829884E-5	0.0
6	3.447563761829884E-5	3.447563761829884E-5	0.0	3.447563761829884E-5	1.723781880914942E-5
7	3.447563761829884E-5	5.1713456427448263E-5	0.0	3.447563761829884E-5	1.723781880914942E-5
8	3.447563761829884E-5	5.1713456427448263E-5	0.0	3.447563761829884E-5	1.723781880914942E-5
9	3.447563761829884E-5	1.0342691285489653E-4	0.0	3.447563761829884E-5	1.723781880914942E-5
10-11	3.447563761829884E-5	1.0342691285489653E-4	0.0	5.1713456427448263E-5	1.723781880914942E-5
12-13	3.447563761829884E-5	1.0342691285489653E-4	0.0	6.033236583202298E-5	1.723781880914942E-5
14-15	5.1713456427448263E-5	1.2066473166404594E-4	0.0	1.0342691285489653E-4	1.723781880914942E-5
16-17	8.61890940457471E-5	1.2066473166404594E-4	0.0	1.0342691285489653E-4	1.723781880914942E-5
18-19	8.61890940457471E-5	1.2066473166404594E-4	0.0	1.0342691285489653E-4	1.723781880914942E-5
20-21	8.61890940457471E-5	1.3790255047319537E-4	0.0	1.2066473166404594E-4	1.723781880914942E-5
22-23	8.61890940457471E-5	1.5514036928234478E-4	0.0	1.2066473166404594E-4	2.5856728213724132E-5
24-25	8.61890940457471E-5	1.896160069006436E-4	0.0	1.4652145987777008E-4	3.447563761829884E-5
26-27	8.61890940457471E-5	2.0685382570979305E-4	0.0	1.9823491630521832E-4	3.447563761829884E-5
28-29	8.61890940457471E-5	2.0685382570979305E-4	0.0	4.3956437963331023E-4	3.447563761829884E-5
30-31	8.61890940457471E-5	2.499483727326666E-4	0.0	0.0015772604210371722	3.447563761829884E-5
32-33	8.61890940457471E-5	2.585672821372413E-4	0.0	0.00400779287312724	5.1713456427448263E-5
34-35	8.61890940457471E-5	3.1028073856468957E-4	0.0	0.007619115913644044	5.1713456427448263E-5
36-37	8.61890940457471E-5	3.619941949921378E-4	0.0	0.013600639040418893	5.1713456427448263E-5
38-39	8.61890940457471E-5	3.792320138012872E-4	0.0	0.028020074474272384	5.1713456427448263E-5
40-41	8.61890940457471E-5	4.137076514195861E-4	0.0	0.0432152117545376	6.033236583202298E-5
42-43	8.61890940457471E-5	4.223265608241608E-4	0.0	0.052652917552546905	6.895127523659768E-5
44-45	8.61890940457471E-5	4.998967454653332E-4	0.0	0.06477972308478352	6.895127523659768E-5
46-47	8.61890940457471E-5	5.688480207019309E-4	0.0	0.07784598974211879	8.61890940457471E-5
48-49	8.61890940457471E-5	5.774669301065056E-4	0.0	0.09044683529160702	8.61890940457471E-5
50-51	8.61890940457471E-5	6.722749335568274E-4	0.0	0.10335796157965993	8.61890940457471E-5
52-53	8.61890940457471E-5	6.895127523659768E-4	0.0	0.14012622909957564	8.61890940457471E-5
54-55	8.61890940457471E-5	6.981316617705515E-4	0.0	0.1869958584416529	9.48080034503218E-5
56-57	8.61890940457471E-5	7.067505711751262E-4	0.0	0.2566107897024028	1.0342691285489653E-4
58-59	8.61890940457471E-5	7.239883899842756E-4	0.0	0.30103264877358094	1.0342691285489653E-4
60-61	9.48080034503218E-5	7.498451181979997E-4	0.0	0.31625364278205986	1.0342691285489653E-4
62-63	1.2066473166404594E-4	7.75701846411724E-4	0.0	0.32669976098040443	1.5514036928234478E-4
64-65	1.2066473166404594E-4	7.929396652208734E-4	0.0	0.33216414954290474	1.637592786869195E-4
66-67	1.2066473166404594E-4	8.274153028391722E-4	0.0	0.3381111970320613	1.809970974960689E-4
68-69	1.2066473166404594E-4	8.274153028391722E-4	0.0	0.3437996772390806	1.9823491630521832E-4
70-71	1.3790255047319537E-4	8.274153028391722E-4	0.0	0.3483763181329098	2.0685382570979305E-4
72-73	1.3790255047319537E-4	8.274153028391722E-4	0.0	0.35237549209663244	2.0685382570979305E-4
74-75	1.3790255047319537E-4	8.446531216483216E-4	0.0	0.35541796711644735	2.2409164451894247E-4
76-77	1.3790255047319537E-4	8.705098498620458E-4	0.0	0.3588482930594681	2.2409164451894247E-4
78-79	1.3790255047319537E-4	8.791287592666205E-4	0.0	0.3611323040516804	2.2409164451894247E-4
80-81	1.3790255047319537E-4	8.791287592666205E-4	0.0	0.36187353026047375	2.2409164451894247E-4
82-83	1.3790255047319537E-4	8.791287592666205E-4	0.0	0.36239066482474824	2.585672821372413E-4
84-85	1.4652145987777008E-4	8.791287592666205E-4	0.0	0.36258028083164895	2.585672821372413E-4
86-87	1.5514036928234478E-4	9.136043968849193E-4	0.0	0.36267508883509925	2.585672821372413E-4
88	1.5514036928234478E-4	9.82555672121517E-4	0.0	0.3626837077445038	2.585672821372413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7545	0.0	49.534935	1
GTATCAA	13480	0.0	34.791058	1
ATCAACG	16895	0.0	28.99187	3
TCAACGC	17010	0.0	28.79562	4
CAACGCA	17390	0.0	28.141937	5
TATCAAC	17690	0.0	27.881245	2
AACGCAG	18010	0.0	27.173141	6
ACGCAGA	20450	0.0	23.930967	7
TGGTATC	3570	0.0	23.581911	2
CGCAGAG	21265	0.0	23.033789	8
GTGGTAT	3880	0.0	22.903221	1
GCAGAGT	24585	0.0	19.888678	9
GAGTACT	14610	0.0	19.556778	12-13
TACATGG	17015	0.0	18.841757	2
CAGAGTA	23860	0.0	18.434795	10-11
GTACATG	16435	0.0	18.290758	1
AGAGTAC	22310	0.0	17.952692	10-11
ACATGGG	17180	0.0	17.819328	3
GTACTTT	16100	0.0	17.575281	14-15
AGTACTT	16020	0.0	16.40228	12-13
>>END_MODULE