FastQCFastQC Report
Fri 27 May 2016
SRR2139613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7242913
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96350.049.9962431
GTATCAA173800.035.67771
ATCAACG215050.029.6067833
TCAACGC215400.029.5758444
CAACGCA220950.028.813615
TATCAAC223950.028.6035672
AACGCAG228450.027.8487786
ACGCAGA260000.024.427
CGCAGAG267500.023.7832138
TGGTATC46750.022.5629222
GTGGTAT51200.021.6027871
GCAGAGT312550.020.5054459
GAGTACT178900.019.02199412-13
CAGAGTA306950.018.54955310-11
AGAGTAC285100.018.26386310-11
GTACATG231250.017.8392011
TACATGG230100.017.8170012
ACATGGG229150.017.2375243
GTACTTT199450.017.06238714-15
AGTACTT195800.016.6387212-13
Produced by FastQC (version 0.11.5)